WO2007100356A3 - A microfluidic interface for highly parallel addressing of sensing arrays - Google Patents

A microfluidic interface for highly parallel addressing of sensing arrays Download PDF

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Publication number
WO2007100356A3
WO2007100356A3 PCT/US2006/038505 US2006038505W WO2007100356A3 WO 2007100356 A3 WO2007100356 A3 WO 2007100356A3 US 2006038505 W US2006038505 W US 2006038505W WO 2007100356 A3 WO2007100356 A3 WO 2007100356A3
Authority
WO
WIPO (PCT)
Prior art keywords
spotter
orifices
spot
orifice
proteins
Prior art date
Application number
PCT/US2006/038505
Other languages
French (fr)
Other versions
WO2007100356A2 (en
Inventor
Bruce K Gale
David Myszka
David A Chang-Yen
Sriram Natarajan
Josh Eckman
Original Assignee
Univ Utah Res Found
Bruce K Gale
David Myszka
David A Chang-Yen
Sriram Natarajan
Josh Eckman
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Utah Res Found, Bruce K Gale, David Myszka, David A Chang-Yen, Sriram Natarajan, Josh Eckman filed Critical Univ Utah Res Found
Publication of WO2007100356A2 publication Critical patent/WO2007100356A2/en
Publication of WO2007100356A3 publication Critical patent/WO2007100356A3/en

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
    • G01N33/553—Metal or metal coated
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1065—Multiple transfer devices
    • G01N35/1074—Multiple transfer devices arranged in a two-dimensional array
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/021—Adjust spacings in an array of wells, pipettes or holders, format transfer between arrays of different size or geometry
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0636—Integrated biosensor, microarrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0819—Microarrays; Biochips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0822—Slides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0877—Flow chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0289—Apparatus for withdrawing or distributing predetermined quantities of fluid
    • B01L3/0293—Apparatus for withdrawing or distributing predetermined quantities of fluid for liquids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
    • G01N2035/00099—Characterised by type of test elements
    • G01N2035/00158—Elements containing microarrays, i.e. "biochip"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N2035/1027—General features of the devices
    • G01N2035/1034—Transferring microquantities of liquid
    • G01N2035/1039—Micropipettes, e.g. microcapillary tubes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/2575—Volumetric liquid transfer

Abstract

Disclosed is a spotter device and methods for the formation of microassays, biochips, biosensors, and cell cultures. The spotter (10) may be used to deposit highly concentrated spots of protein or other materials on a microarray slide, wafer, or other surface. It may also be used to perform various chemistry steps on the same spots. The spotter increases the surface density of substances at each spot by directing a flow the desired substance (or a solution thereof) over the spot area until surface saturation is accomplished. The spotter may be loaded by well plate handling equipment. The spotter uses wells (22, 24), microfluidic conduits, and orifices to deposit proteins, other biomolecules, or chemicals on a spot on a separate surface. Each orifice is connect to two wells via microconduits (26, 28). When the spotter contacts a surface (30), a seal is formed between th orifices (27) and the surface. The same or different substances may be flowed across each orifice. Any numb of orifices may be incorporated into a spotter. The spotter is particularly useful for depositing proteins in high concentrations on a surface, since the spotter may be placed on a surface for an extended period of time.
PCT/US2006/038505 2005-09-30 2006-10-02 A microfluidic interface for highly parallel addressing of sensing arrays WO2007100356A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11/241,060 2005-09-30
US11/241,060 US8383059B2 (en) 2005-09-30 2005-09-30 Microfluidic interface for highly parallel addressing of sensing arrays

Publications (2)

Publication Number Publication Date
WO2007100356A2 WO2007100356A2 (en) 2007-09-07
WO2007100356A3 true WO2007100356A3 (en) 2007-12-13

Family

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PCT/US2006/038505 WO2007100356A2 (en) 2005-09-30 2006-10-02 A microfluidic interface for highly parallel addressing of sensing arrays

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US (2) US8383059B2 (en)
WO (1) WO2007100356A2 (en)

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9976192B2 (en) 2006-03-10 2018-05-22 Ldip, Llc Waveguide-based detection system with scanning light source
US7951583B2 (en) 2006-03-10 2011-05-31 Plc Diagnostics, Inc. Optical scanning system
US9528939B2 (en) 2006-03-10 2016-12-27 Indx Lifecare, Inc. Waveguide-based optical scanning systems
US9423397B2 (en) 2006-03-10 2016-08-23 Indx Lifecare, Inc. Waveguide-based detection system with scanning light source
US8288157B2 (en) 2007-09-12 2012-10-16 Plc Diagnostics, Inc. Waveguide-based optical scanning systems
WO2008154225A2 (en) * 2007-06-06 2008-12-18 Bayer Healthcare Llc Microdeposition system for a biosensor
GB2461026B (en) 2008-06-16 2011-03-09 Plc Diagnostics Inc System and method for nucleic acids sequencing by phased synthesis
US9682372B2 (en) 2008-06-19 2017-06-20 Carterra, Inc. Tip overlay for continuous flow spotting apparatus
WO2010034010A2 (en) * 2008-09-22 2010-03-25 Wasatch Microfluidics Microassay with internal referencing
KR101602414B1 (en) * 2009-03-16 2016-03-11 삼성전자주식회사 Apparatus for fluidic control
CN102460254B (en) 2009-04-29 2015-05-06 Plc诊断股份有限公司 Waveguide-based detection system with scanning light source
NL2002868C2 (en) * 2009-05-11 2010-11-15 Ibis Technologies B V Method for the determination of competing interactants binding to a compound, use, combination and apparatus.
US20130025690A1 (en) * 2011-07-29 2013-01-31 Intermolecular, Inc. No-Contact Wet Processing Tool with Liquid Barrier
TWI432727B (en) * 2011-04-28 2014-04-01 Ind Tech Res Inst Apparatus and method for manufacturing microarray biochip
US10161007B2 (en) 2012-08-13 2018-12-25 The Regents Of The University Of California Methods and systems for detecting biological components
US10300450B2 (en) 2012-09-14 2019-05-28 Carterra, Inc. Method and device for depositing a substance on a submerged surface
WO2014127250A1 (en) * 2013-02-14 2014-08-21 Cfd Research Corporation Cell culture device with an array of microfluidic networks
WO2014138132A2 (en) * 2013-03-04 2014-09-12 Verax Biomedical Incorporated Multi-analyte assay
US10018566B2 (en) 2014-02-28 2018-07-10 Ldip, Llc Partially encapsulated waveguide based sensing chips, systems and methods of use
US9682396B2 (en) 2014-03-24 2017-06-20 Carterra, Inc. Dual flow cell fluid delivery systems
EP3160654A4 (en) 2014-06-27 2017-11-15 The Regents of The University of California Pcr-activated sorting (pas)
EP3209419A4 (en) * 2014-10-22 2018-10-03 The Regents of The University of California High definition microdroplet printer
CA2974306A1 (en) 2015-02-04 2016-08-11 The Regents Of The University Of California Sequencing of nucleic acids via barcoding in discrete entities
US11181479B2 (en) 2015-02-27 2021-11-23 Ldip, Llc Waveguide-based detection system with scanning light source
TWI552808B (en) * 2015-06-09 2016-10-11 緯創資通股份有限公司 Pipette
GB201516992D0 (en) * 2015-09-25 2015-11-11 Ge Healthcare Bio Sciences Ab Method and system for evaluation of an interaction between an analyte and a ligand using a biosensor
WO2017210199A1 (en) * 2016-05-31 2017-12-07 Oregon State University Fluidic devices for chromatographic separation and methods of making and using the same
WO2018031691A1 (en) 2016-08-10 2018-02-15 The Regents Of The University Of California Combined multiple-displacement amplification and pcr in an emulsion microdroplet
WO2018119301A1 (en) 2016-12-21 2018-06-28 The Regents Of The University Of California Single cell genomic sequencing using hydrogel based droplets
US10753954B2 (en) 2017-07-11 2020-08-25 International Business Machines Corporation Vacuum-driven microfluidic probes
US10501739B2 (en) 2017-10-18 2019-12-10 Mission Bio, Inc. Method, systems and apparatus for single cell analysis
US11207455B2 (en) 2018-05-14 2021-12-28 Oregon State University Membrane device for blood separation and methods of making and using the same
CA3138806A1 (en) 2019-05-22 2020-11-26 Dalia Dhingra Method and apparatus for simultaneous targeted sequencing of dna, rna and protein
WO2021003255A1 (en) 2019-07-01 2021-01-07 Mission Bio Method and apparatus to normalize quantitative readouts in single-cell experiments
WO2023194510A1 (en) * 2022-04-05 2023-10-12 Vysens B.V. Method and device for depositing a compound in a gradient on a sensor surface

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6274091B1 (en) * 1995-09-22 2001-08-14 Berlex Laboratories, Inc. Apparatus and process for multiple chemical reactions
US20050047962A1 (en) * 2001-12-11 2005-03-03 Astrazeneca Ab Ffe array dispenser

Family Cites Families (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5807522A (en) 1994-06-17 1998-09-15 The Board Of Trustees Of The Leland Stanford Junior University Methods for fabricating microarrays of biological samples
US5811306A (en) * 1995-09-04 1998-09-22 Fuji Photo Film Co., Ltd. Liquid spotting method
US6699719B2 (en) 1996-11-29 2004-03-02 Proteomic Systems, Inc. Biosensor arrays and methods
US6090251A (en) * 1997-06-06 2000-07-18 Caliper Technologies, Inc. Microfabricated structures for facilitating fluid introduction into microfluidic devices
EP0996500A1 (en) 1997-07-22 2000-05-03 Rapigene, Inc. Apparatus and methods for arraying solution onto a solid support
WO1999014368A2 (en) * 1997-09-15 1999-03-25 Whitehead Institute For Biomedical Research Methods and apparatus for processing a sample of biomolecular analyte using a microfabricated device
US6242246B1 (en) 1997-12-15 2001-06-05 Somalogic, Inc. Nucleic acid ligand diagnostic Biochip
JP3394181B2 (en) 1998-03-30 2003-04-07 日立ソフトウエアエンジニアリング株式会社 Sample addition method and sample addition device
EP0955084B1 (en) * 1998-04-27 2006-07-26 Corning Incorporated Method of depositing an array of biological samples using a redrawn capillary reservoir
US6884626B1 (en) * 1998-04-27 2005-04-26 Corning Incorporated Redrawn capillary imaging reservoir
US6762061B1 (en) * 1998-07-03 2004-07-13 Corning Incorporated Redrawn capillary imaging reservoir
US6461812B2 (en) * 1998-09-09 2002-10-08 Agilent Technologies, Inc. Method and multiple reservoir apparatus for fabrication of biomolecular arrays
US6627157B1 (en) * 1999-03-04 2003-09-30 Ut-Battelle, Llc Dual manifold system and method for fluid transfer
US6673620B1 (en) * 1999-04-20 2004-01-06 Cytologix Corporation Fluid exchange in a chamber on a microscope slide
US6485690B1 (en) * 1999-05-27 2002-11-26 Orchid Biosciences, Inc. Multiple fluid sample processor and system
KR100379411B1 (en) 1999-06-28 2003-04-10 엘지전자 주식회사 biochip and method for patterning and measuring biomaterial of the same
CA2391758C (en) * 1999-08-13 2010-02-16 Cartesian Technologies, Inc. Apparatus for liquid sample handling
KR100339379B1 (en) 1999-10-29 2002-06-03 구자홍 biochip and apparatus and method for measuring biomaterial of the same
US20040014102A1 (en) 2000-02-22 2004-01-22 Shiping Chen High density parallel printing of microarrays
CN1404415A (en) 2000-02-22 2003-03-19 基因谱公司 Microarray fabrication techniques and apparatus
DE10010208C2 (en) * 2000-02-25 2002-02-07 Inst Physikalische Hochtech Ev Microdosing device for the defined delivery of small, closed liquid volumes
JP3502803B2 (en) 2000-03-06 2004-03-02 日立ソフトウエアエンジニアリング株式会社 Microarray, method for producing microarray, and method for correcting spot amount error between pins in microarray
WO2001074490A2 (en) * 2000-04-03 2001-10-11 Ecole Polytechnique Federale De Lausanne (Epfl) A device for dispensing accurately-controlled small doses of liquid
CA2311622A1 (en) * 2000-06-15 2001-12-15 Moussa Hoummady Sub-nanoliter liquid drop dispensing system and method therefor
US6656740B1 (en) * 2000-10-31 2003-12-02 Agilent Technologies, Inc. Pressure variation in array fabrication
US7166258B2 (en) 2001-01-31 2007-01-23 Agilent Technologies, Inc. Automation-optimized microarray package
US20020164824A1 (en) * 2001-02-16 2002-11-07 Jianming Xiao Method and apparatus based on bundled capillaries for high throughput screening
WO2002102514A1 (en) * 2001-06-19 2002-12-27 B.C. Cancer Agency Microvolume liquid dispenser suitable for microarrays and methods related thereto
US7402286B2 (en) * 2001-06-27 2008-07-22 The Regents Of The University Of California Capillary pins for high-efficiency microarray printing device
EP1323474A1 (en) * 2001-12-31 2003-07-02 Corning Incorporated Device and process for simultaneous transfer of liquids
US20040109793A1 (en) * 2002-02-07 2004-06-10 Mcneely Michael R Three-dimensional microfluidics incorporating passive fluid control structures
US20050266582A1 (en) * 2002-12-16 2005-12-01 Modlin Douglas N Microfluidic system with integrated permeable membrane
US7025935B2 (en) * 2003-04-11 2006-04-11 Illumina, Inc. Apparatus and methods for reformatting liquid samples
US20060233673A1 (en) * 2003-09-19 2006-10-19 Beard Nigel P High density plate filler
US20050249641A1 (en) * 2004-04-08 2005-11-10 Boehringer Ingelheim Microparts Gmbh Microstructured platform and method for manipulating a liquid
WO2005121746A1 (en) * 2004-06-09 2005-12-22 The University Of British Columbia Reagent delivery apparatus and methods
EP1776592A1 (en) * 2004-07-22 2007-04-25 Amgen, Inc Method and apparatus for forming a dilution by fluid dispersion
FR2917165B1 (en) * 2007-06-08 2009-10-02 Novacyt Soc Par Actions Simpli DEVICE FOR DEPOSITING CELLS BY DECANTATION ON A PLATE OF ANALYSIS
US9682372B2 (en) * 2008-06-19 2017-06-20 Carterra, Inc. Tip overlay for continuous flow spotting apparatus
WO2010034010A2 (en) * 2008-09-22 2010-03-25 Wasatch Microfluidics Microassay with internal referencing
US10300450B2 (en) * 2012-09-14 2019-05-28 Carterra, Inc. Method and device for depositing a substance on a submerged surface

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6274091B1 (en) * 1995-09-22 2001-08-14 Berlex Laboratories, Inc. Apparatus and process for multiple chemical reactions
US20050047962A1 (en) * 2001-12-11 2005-03-03 Astrazeneca Ab Ffe array dispenser

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Publication number Publication date
US8383059B2 (en) 2013-02-26
WO2007100356A2 (en) 2007-09-07
US20070231880A1 (en) 2007-10-04
US20140235510A1 (en) 2014-08-21
US8999726B2 (en) 2015-04-07

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