WO2007114931A3 - Nanoelectronic detection of biomolecules employing analyte amplification and reporters - Google Patents

Nanoelectronic detection of biomolecules employing analyte amplification and reporters Download PDF

Info

Publication number
WO2007114931A3
WO2007114931A3 PCT/US2007/008422 US2007008422W WO2007114931A3 WO 2007114931 A3 WO2007114931 A3 WO 2007114931A3 US 2007008422 W US2007008422 W US 2007008422W WO 2007114931 A3 WO2007114931 A3 WO 2007114931A3
Authority
WO
WIPO (PCT)
Prior art keywords
reporters
detection
biomolecules
nanoelectronic
analyte amplification
Prior art date
Application number
PCT/US2007/008422
Other languages
French (fr)
Other versions
WO2007114931A2 (en
Inventor
Eugene Tu
Christian Valcke
Original Assignee
Nanomix Inc
Eugene Tu
Christian Valcke
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US11/488,465 external-priority patent/US20070045756A1/en
Priority claimed from US11/588,845 external-priority patent/US20080021339A1/en
Application filed by Nanomix Inc, Eugene Tu, Christian Valcke filed Critical Nanomix Inc
Priority to JP2009504284A priority Critical patent/JP2009532064A/en
Priority to EP07754869A priority patent/EP2007379A4/en
Publication of WO2007114931A2 publication Critical patent/WO2007114931A2/en
Publication of WO2007114931A3 publication Critical patent/WO2007114931A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0645—Electrodes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5029—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures using swabs

Abstract

Methods of detection of biomolecules are described, including methods of amplification of analyte target species and target reporters by analyte-triggered action of an enzyme such a nuclease, polymerase, and the like. Amplified target species (e.g., amplicons and reporters) are detectable by several embodiments of nanoelectronic sensors having aspects of the invention, and by alternative convention biomolecule detection methods.
PCT/US2007/008422 2006-04-04 2007-04-03 Nanoelectronic detection of biomolecules employing analyte amplification and reporters WO2007114931A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2009504284A JP2009532064A (en) 2006-04-04 2007-04-03 Nanoelectronic detection of biomolecules using analyte amplification and reporter
EP07754869A EP2007379A4 (en) 2006-04-04 2007-04-03 Nanoelectronic detection of biomolecules employing analyte amplification and reporters

Applications Claiming Priority (10)

Application Number Priority Date Filing Date Title
US78902206P 2006-04-04 2006-04-04
US60/789,022 2006-04-04
US11/488,465 US20070045756A1 (en) 2002-09-04 2006-07-18 Nanoelectronic sensor with integral suspended micro-heater
US11/488,465 2006-07-18
US85021706P 2006-10-06 2006-10-06
US60/850,217 2006-10-06
US11/588,845 US20080021339A1 (en) 2005-10-27 2006-10-26 Anesthesia monitor, capacitance nanosensors and dynamic sensor sampling method
US11/588,845 2006-10-26
US90153807P 2007-02-14 2007-02-14
US60/901,538 2007-02-14

Publications (2)

Publication Number Publication Date
WO2007114931A2 WO2007114931A2 (en) 2007-10-11
WO2007114931A3 true WO2007114931A3 (en) 2008-12-24

Family

ID=38564139

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/008422 WO2007114931A2 (en) 2006-04-04 2007-04-03 Nanoelectronic detection of biomolecules employing analyte amplification and reporters

Country Status (3)

Country Link
EP (1) EP2007379A4 (en)
JP (1) JP2009532064A (en)
WO (1) WO2007114931A2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8154093B2 (en) 2002-01-16 2012-04-10 Nanomix, Inc. Nano-electronic sensors for chemical and biological analytes, including capacitance and bio-membrane devices
US8152991B2 (en) 2005-10-27 2012-04-10 Nanomix, Inc. Ammonia nanosensors, and environmental control system
US7714398B2 (en) 2002-09-05 2010-05-11 Nanomix, Inc. Nanoelectronic measurement system for physiologic gases and improved nanosensor for carbon dioxide
US7522040B2 (en) 2004-04-20 2009-04-21 Nanomix, Inc. Remotely communicating, battery-powered nanostructure sensor devices
US7948041B2 (en) 2005-05-19 2011-05-24 Nanomix, Inc. Sensor having a thin-film inhibition layer
JP2013501921A (en) 2009-08-07 2013-01-17 ナノミックス・インコーポレーテッド Biological detection based on magnetic carbon nanotubes
CN105144177B (en) 2013-04-24 2019-05-28 费森尤斯卡比德国有限公司 A kind of control device controlled for the infusion device to patient's dispensing drug

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5759773A (en) * 1993-01-15 1998-06-02 The Public Health Research Institute Of The City Of New York, Inc. Sensitive nucleic acid sandwich hybridization assay
US20040033525A1 (en) * 1996-12-10 2004-02-19 Monforte Joseph A. Releasable nonvolatile mass-label molecules
US20060014172A1 (en) * 2004-05-03 2006-01-19 Nanosphere, Inc. Aptamer-nanoparticle conjugates and method of use for target analyte detection
US20060035215A9 (en) * 1999-10-29 2006-02-16 Sorge Joseph A Methods for detection of a target nucleic acid by capture
US20060040294A1 (en) * 1996-01-24 2006-02-23 Prudent James R Cleavage of nucleic acids

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030036065A1 (en) * 2001-08-17 2003-02-20 Robert Gellibolian Method and system for array signal generation and amplification
AU2003231058A1 (en) * 2002-04-22 2003-11-03 Wisconsin Alumni Research Foundation Single nucleotide polymorphism analysis using surface invasive cleavage reactions
US20040214176A1 (en) * 2003-04-22 2004-10-28 Osborne James C. Multiplexed DNA assays using structure-specific endonucleases
JP2007528213A (en) * 2003-11-06 2007-10-11 ユニバーシティ・オブ・ネバダ・リノ Improved method for detection and measurement of specific nucleic acid sequences
WO2006071895A2 (en) * 2004-12-28 2006-07-06 Nanomix, Inc. Nanoelectronic devices for dna detection, and recognition of polynucleotide sequences
EP1781771A2 (en) * 2004-08-24 2007-05-09 Nanomix, Inc. Nanotube sensor devices for dna detection

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5759773A (en) * 1993-01-15 1998-06-02 The Public Health Research Institute Of The City Of New York, Inc. Sensitive nucleic acid sandwich hybridization assay
US20060040294A1 (en) * 1996-01-24 2006-02-23 Prudent James R Cleavage of nucleic acids
US20040033525A1 (en) * 1996-12-10 2004-02-19 Monforte Joseph A. Releasable nonvolatile mass-label molecules
US20060035215A9 (en) * 1999-10-29 2006-02-16 Sorge Joseph A Methods for detection of a target nucleic acid by capture
US20060014172A1 (en) * 2004-05-03 2006-01-19 Nanosphere, Inc. Aptamer-nanoparticle conjugates and method of use for target analyte detection

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
See also references of EP2007379A4 *
STAR ET AL.: "Label-free detection of DNA hybridization using carbon nanotube network field-effect transistors", PROC. NATL. ACAD. SCI. USA, vol. 103, no. 4, 24 January 2006 (2006-01-24), pages 921 - 926, XP003023787 *

Also Published As

Publication number Publication date
JP2009532064A (en) 2009-09-10
EP2007379A4 (en) 2009-12-09
EP2007379A2 (en) 2008-12-31
WO2007114931A2 (en) 2007-10-11

Similar Documents

Publication Publication Date Title
WO2007114931A3 (en) Nanoelectronic detection of biomolecules employing analyte amplification and reporters
WO2010019414A3 (en) Detecting nucleic acid
WO2007044427A3 (en) Detection of nucleic acids from whole blood
WO2008005321A3 (en) Systems and methods for real-time pcr
WO2007058898A3 (en) Detection of nucleic acids through amplification of surrogate nucleic acids
WO2007056250A3 (en) Heterogeneous assay of analytes in solution using polymers
WO2007098279A3 (en) Analyte detection using barcoded polymers
WO2008104794A3 (en) Nucleic acid detection
EP1943353A4 (en) Assay for a health state
WO2013006793A3 (en) Direct amplification and detection of viral and bacterial pathogens
WO2006113466A8 (en) Methods and nucleic acids for analyses of cellular proliferative disorders
WO2009018003A3 (en) Detection assays and use thereof
WO2009017861A3 (en) Analyte detection using autocatalytic chain reactions
WO2008134470A3 (en) Methods for nucleic acid amplification
WO2009017878A3 (en) High specificity and high sensitivity detection based on steric hindrance & enzyme-related signal amplification
WO2009014787A3 (en) Multianalyte assay
WO2007011867A3 (en) Fluid processing device and method
WO2006117161A3 (en) New labelling strategies for the sensitive detection of analytes
WO2006117676A3 (en) Method for the detection and characterization of microorganisms on a filter
WO2008085538A3 (en) New assay platforms and detection methodology using surface enhanced raman scattering (sers) upon specific biochemical interactions
WO2011100749A3 (en) Methods and materials for detecting viral or microbial infections
WO2010065781A3 (en) Lateral flow strip assay with immobilized conjugate
GB0500996D0 (en) Detection method and materials therefor
WO2006135904A3 (en) Method for producing improved results for applications which directly or indirectly utilize gene expression assay results
WO2007030737A3 (en) Film thin waveguides, methods of fabrication thereof, and detection systems

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 07754869

Country of ref document: EP

Kind code of ref document: A2

WWE Wipo information: entry into national phase

Ref document number: 2009504284

Country of ref document: JP

NENP Non-entry into the national phase

Ref country code: DE

WWE Wipo information: entry into national phase

Ref document number: 2007754869

Country of ref document: EP