WO2007115123A3 - Mineralized three-dimensional bone constructs - Google Patents
Mineralized three-dimensional bone constructs Download PDFInfo
- Publication number
- WO2007115123A3 WO2007115123A3 PCT/US2007/065542 US2007065542W WO2007115123A3 WO 2007115123 A3 WO2007115123 A3 WO 2007115123A3 US 2007065542 W US2007065542 W US 2007065542W WO 2007115123 A3 WO2007115123 A3 WO 2007115123A3
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- WO
- WIPO (PCT)
- Prior art keywords
- bone
- constructs
- bone constructs
- mineralized
- gravity vector
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3839—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by the site of application in the body
- A61L27/3843—Connective tissue
- A61L27/3847—Bones
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
- A61L27/3821—Bone-forming cells, e.g. osteoblasts, osteocytes, osteoprogenitor cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3886—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells comprising two or more cell types
- A61L27/3891—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells comprising two or more cell types as distinct cell layers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3895—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells using specific culture conditions, e.g. stimulating differentiation of stem cells, pulsatile flow conditions
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0654—Osteocytes, Osteoblasts, Odontocytes; Bones, Teeth
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/02—Materials or treatment for tissue regeneration for reconstruction of bones; weight-bearing implants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/42—Organic phosphate, e.g. beta glycerophosphate
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/20—Cytokines; Chemokines
- C12N2501/22—Colony stimulating factors (G-CSF, GM-CSF)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/30—Hormones
- C12N2501/38—Hormones with nuclear receptors
- C12N2501/39—Steroid hormones
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/60—Transcription factors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/11—Coculture with; Conditioned medium produced by blood or immune system cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2525/00—Culture process characterised by gravity, e.g. microgravity
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Cell Biology (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Epidemiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Dermatology (AREA)
- Medicinal Chemistry (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Transplantation (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Botany (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Orthopedic Medicine & Surgery (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Developmental Biology & Embryology (AREA)
- Urology & Nephrology (AREA)
- Vascular Medicine (AREA)
- Rheumatology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Materials For Medical Uses (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The present disclosure provides ex vivo-derived mineralized three-dimensional bone constructs. The bone constructs are obtained by culturing osteoblasts and osteoclast precursors under randomized gravity vector conditions. Preferably, the randomized gravity vector conditions are obtained using a low shear stress rotating bioreactor, such as a High Aspect Ratio Vessel (HARV) culture system. The bone constructs of the disclosure have utility in physiological studies of bone formation and bone function, in drug discovery, and in orthopedics.
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AT07759733T ATE531792T1 (en) | 2006-03-30 | 2007-03-29 | THREE-DIMENSIONAL MINERALIZED BONE CONSTRUCTS |
ES07759733T ES2376222T3 (en) | 2006-03-30 | 2007-03-29 | MINERALIZED THREE-DIMENSIONAL BONE CONSTRUCTIONS. |
EP07759733A EP2013730B1 (en) | 2006-03-30 | 2007-03-29 | Mineralized three-dimensional bone constructs |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US78743106P | 2006-03-30 | 2006-03-30 | |
US60/787,431 | 2006-03-30 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2007115123A2 WO2007115123A2 (en) | 2007-10-11 |
WO2007115123A3 true WO2007115123A3 (en) | 2009-04-09 |
Family
ID=38564225
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2007/065542 WO2007115123A2 (en) | 2006-03-30 | 2007-03-29 | Mineralized three-dimensional bone constructs |
Country Status (5)
Country | Link |
---|---|
US (3) | US8076136B2 (en) |
EP (1) | EP2013730B1 (en) |
AT (1) | ATE531792T1 (en) |
ES (1) | ES2376222T3 (en) |
WO (1) | WO2007115123A2 (en) |
Families Citing this family (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2007115123A2 (en) * | 2006-03-30 | 2007-10-11 | Mark Clarke | Mineralized three-dimensional bone constructs |
WO2009064956A1 (en) * | 2007-11-14 | 2009-05-22 | Osteosphere, Llc | A novel tissue culture platform for screening of potential bone remodeling agents |
WO2009064957A1 (en) | 2007-11-14 | 2009-05-22 | Osteosphere, Llc | Development of a human colloidal bone graft material |
WO2009064917A2 (en) * | 2007-11-14 | 2009-05-22 | Osteosphere, Llc | Generation of an hla-negative osteogenic precursor cell line |
US20110082549A1 (en) * | 2008-02-01 | 2011-04-07 | Osteosphere, Llc | Mineralized three-dimensional bone constructs formed from osteoblasts |
ES2775977T3 (en) | 2008-04-10 | 2020-07-28 | Bonus Therapeutics Ltd | Prosthetic bone-like implants |
US8278101B2 (en) * | 2009-12-07 | 2012-10-02 | Synthecon, Inc. | Stem cell bioprocessing and cell expansion |
IL204116A (en) * | 2010-02-23 | 2013-10-31 | Sheltagen Medical Ltd | Three-dimensional bone implant and method for producing same |
US9110282B2 (en) * | 2011-03-30 | 2015-08-18 | The Regents Of The University Of California | Nanometer-scale optical imaging by the modulation tracking (MT) method |
WO2015155777A1 (en) | 2014-04-10 | 2015-10-15 | Bonus Therapeutics Ltd. | Bone repair compositions |
US20200308524A1 (en) * | 2017-10-10 | 2020-10-01 | Temple University-of The Commonwealth SAystem of Higher Education | Device and Method for Enhanced Air Bubble Removal from Rotary Bioreactor Cell Cultures and Culture Chambers |
KR102648746B1 (en) * | 2020-05-13 | 2024-03-18 | 한국기초과학지원연구원 | Bone tissue mimetics and method for preparing the same |
WO2023085837A1 (en) * | 2021-11-11 | 2023-05-19 | 한국기초과학지원연구원 | Method and device for screening for osteoporosis drug by using bone tissue mimetic image analysis |
KR102531759B1 (en) * | 2021-11-11 | 2023-05-12 | 한국기초과학지원연구원 | Method and Apparatus for Screening Osteoporosis Drug Based on Image Analysis of Bone Tissue Mimetics |
Citations (1)
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WO2005012512A1 (en) * | 2003-08-01 | 2005-02-10 | Norimasa Nakamura | Scaffold-free self-organized 3d synthetic tissue |
Family Cites Families (27)
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US5026650A (en) | 1988-06-30 | 1991-06-25 | The United States Of Amercia As Represented By The Administrator Of The National Aeronautics And Space Administration | Horizontally rotated cell culture system with a coaxial tubular oxygenator |
US5496722A (en) | 1988-06-30 | 1996-03-05 | The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration | Method for producing non-neoplastic, three dimensional, mammalian tissue and cell aggregates under microgravity culture conditions and the products produced therefrom |
US5962324A (en) | 1988-06-30 | 1999-10-05 | The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Adminstration | Three dimensional optic tissue culture and process |
US5155034A (en) | 1988-06-30 | 1992-10-13 | The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration | Three-dimensional cell to tissue assembly process |
US5851816A (en) | 1988-06-30 | 1998-12-22 | The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration | Cultured high-fidelity three-dimensional human urogenital tract carcinomas and process |
US5308764A (en) | 1988-06-30 | 1994-05-03 | The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration | Multi-cellular, three-dimensional living mammalian tissue |
US4988623A (en) | 1988-06-30 | 1991-01-29 | The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration | Rotating bio-reactor cell culture apparatus |
US5153151A (en) | 1991-05-06 | 1992-10-06 | Corning Incorporated | High index fluorine-free phosphate glasses |
US5858783A (en) | 1993-05-25 | 1999-01-12 | The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration | Production of normal mammalian organ culture using a medium containing mem-alpha, leibovitz L-15, glucose galactose fructose |
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US5972703A (en) * | 1994-08-12 | 1999-10-26 | The Regents Of The University Of Michigan | Bone precursor cells: compositions and methods |
US5827729A (en) | 1996-04-23 | 1998-10-27 | Advanced Tissue Sciences | Diffusion gradient bioreactor and extracorporeal liver device using a three-dimensional liver tissue |
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KR100563476B1 (en) | 1998-07-03 | 2006-03-27 | 이진용 | Bone regeneration material |
US6107081A (en) | 1999-02-05 | 2000-08-22 | The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration | Uni-directional cell stretching device |
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US7291345B2 (en) * | 2002-12-12 | 2007-11-06 | Osteotech, Inc. | Formable and settable polymer bone composite and method of production thereof |
US7494811B2 (en) | 2003-05-01 | 2009-02-24 | Lifenet Health | In vitro growth of tissues suitable to the formation of bone and bone forming tissue formed thereby |
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WO2007115123A2 (en) * | 2006-03-30 | 2007-10-11 | Mark Clarke | Mineralized three-dimensional bone constructs |
WO2009064957A1 (en) * | 2007-11-14 | 2009-05-22 | Osteosphere, Llc | Development of a human colloidal bone graft material |
-
2007
- 2007-03-29 WO PCT/US2007/065542 patent/WO2007115123A2/en active Application Filing
- 2007-03-29 EP EP07759733A patent/EP2013730B1/en not_active Not-in-force
- 2007-03-29 US US11/693,662 patent/US8076136B2/en active Active
- 2007-03-29 ES ES07759733T patent/ES2376222T3/en active Active
- 2007-03-29 AT AT07759733T patent/ATE531792T1/en active
-
2010
- 2010-05-17 US US12/781,482 patent/US8557576B2/en active Active
-
2011
- 2011-11-11 US US13/294,878 patent/US20120058557A1/en not_active Abandoned
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2005012512A1 (en) * | 2003-08-01 | 2005-02-10 | Norimasa Nakamura | Scaffold-free self-organized 3d synthetic tissue |
Non-Patent Citations (5)
Title |
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FACER, S.R. ET AL.: "Rotary culture enhances pre-osteoblast aggregation and mineralization.", J. DENT. RES., vol. 84, no. 6, 2005, pages 542 - 547, XP008131896 * |
GLANTSCHNIG ET AL.: "M-CSF, TNFa and RANK ligand promote osteoclast survival by signaling through mTOR/S6 kinase.", CELL DEATH AND DIFFERENTIATION., vol. 10, 2003, pages 1165 - 1177, XP008130560 * |
MARLOVITS ET AL.: "Chondrogenesis of Aged Human Articular Cartilage in a Scaffold-Free Bioreactor.", TISSUE ENGINEERING, vol. 9, no. 6, 2003, pages 1215 - 1226, XP002534525 * |
NAKAGAWA ET AL.: "Osteoclastogenesis on Tissue-Engineered Bone. pg 93-100", TISSUE ENGINEERING, vol. 10, no. 1/2, 2004, XP008131897 * |
YU ET AL.: "Bioreactor-based bone tissue engineering: the influence of dynamic flow on osteoblast phenotypic expression and matrix mineralization.", PNAS, vol. 101, no. 31, 2004, pages 11203 - 11208, XP008130559 * |
Also Published As
Publication number | Publication date |
---|---|
WO2007115123A2 (en) | 2007-10-11 |
US20120058557A1 (en) | 2012-03-08 |
ATE531792T1 (en) | 2011-11-15 |
US8076136B2 (en) | 2011-12-13 |
US20100227358A1 (en) | 2010-09-09 |
ES2376222T3 (en) | 2012-03-12 |
EP2013730A2 (en) | 2009-01-14 |
US8557576B2 (en) | 2013-10-15 |
US20090061479A1 (en) | 2009-03-05 |
EP2013730A4 (en) | 2010-05-26 |
EP2013730B1 (en) | 2011-11-02 |
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