WO2007120166A3 - Miniaturized in vitro protein expression array - Google Patents

Miniaturized in vitro protein expression array Download PDF

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Publication number
WO2007120166A3
WO2007120166A3 PCT/US2006/026752 US2006026752W WO2007120166A3 WO 2007120166 A3 WO2007120166 A3 WO 2007120166A3 US 2006026752 W US2006026752 W US 2006026752W WO 2007120166 A3 WO2007120166 A3 WO 2007120166A3
Authority
WO
WIPO (PCT)
Prior art keywords
array
functions
detection
miniaturized
sensor
Prior art date
Application number
PCT/US2006/026752
Other languages
French (fr)
Other versions
WO2007120166A2 (en
Inventor
Zhonghui Hugh Fan
Shuguang Jin
Qian Mei
Original Assignee
Univ Florida
Zhonghui Hugh Fan
Shuguang Jin
Qian Mei
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Florida, Zhonghui Hugh Fan, Shuguang Jin, Qian Mei filed Critical Univ Florida
Publication of WO2007120166A2 publication Critical patent/WO2007120166A2/en
Publication of WO2007120166A3 publication Critical patent/WO2007120166A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/08—Ergonomic or safety aspects of handling devices
    • B01L2200/082—Handling hazardous material
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B01L2200/142—Preventing evaporation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0654—Lenses; Optical fibres
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0663—Whole sensors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0867—Multiple inlets and one sample wells, e.g. mixing, dilution
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0472—Diffusion

Abstract

Detection of inhibition of biological synthesis of pre-characterized proteins indicates presence of a toxic analyte. The sensor array comprises an array of units; each unit is for expression of one protein and thus functions as one sensor. The sensor array expresses a group of pre-characterized proteins in different expression system, thus the response pattern (or signature) of an analyte due to different inhibitory effects is registered and used as a tool for detection and identification. New agents are identified by comparing the response pattern with signatures of known agents in a pre-collected database. The protein synthesis array device also functions as surrogate cells, which can be used for systematic and quantitative studies of the interplay among a large number of the constituents in a biological cell, for studying molecular responses to toxins, and for search for the therapeutic targets.
PCT/US2006/026752 2005-07-11 2006-07-11 Miniaturized in vitro protein expression array WO2007120166A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US69804405P 2005-07-11 2005-07-11
US60/698,044 2005-07-11

Publications (2)

Publication Number Publication Date
WO2007120166A2 WO2007120166A2 (en) 2007-10-25
WO2007120166A3 true WO2007120166A3 (en) 2008-12-04

Family

ID=38609939

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/026752 WO2007120166A2 (en) 2005-07-11 2006-07-11 Miniaturized in vitro protein expression array

Country Status (1)

Country Link
WO (1) WO2007120166A2 (en)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106290852A (en) * 2015-05-11 2017-01-04 宁波大学 Can the AIDS diagnosis device of Dual Drive CGCM of rapid disassembling
CN106290869A (en) * 2015-05-11 2017-01-04 宁波大学 The controller used in syphilis diagnosis micro fluidic device of the Dual Drive CGCM of easily-disassembled framework
CN106290851A (en) * 2015-05-11 2017-01-04 宁波大学 A kind of easily-disassembled cholera diagnosis micro fluidic device of Dual Drive CGCM
CN106290929A (en) * 2015-05-11 2017-01-04 宁波大学 The hypotype swine flue detection device of the Dual Drive CGCM that can disassemble rapidly
CN106290853A (en) * 2015-05-11 2017-01-04 宁波大学 Take the AIDS diagnosis device of the Dual Drive CGCM of easily-disassembled framework

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX2022001468A (en) 2019-08-09 2022-07-19 Nutcracker Therapeutics Inc Methods and apparatuses for manufacturing for removing material from a therapeutic composition.

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020177614A1 (en) * 2001-03-23 2002-11-28 Merril Carl R. Methods for treating nuerodegenerative diseases including alzheimer's
US20030157643A1 (en) * 2000-08-24 2003-08-21 Almond Brian D Synthetic nucleic acids from aquatic species
US6635226B1 (en) * 1994-10-19 2003-10-21 Agilent Technologies, Inc. Microanalytical device and use thereof for conducting chemical processes

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6635226B1 (en) * 1994-10-19 2003-10-21 Agilent Technologies, Inc. Microanalytical device and use thereof for conducting chemical processes
US20030157643A1 (en) * 2000-08-24 2003-08-21 Almond Brian D Synthetic nucleic acids from aquatic species
US20020177614A1 (en) * 2001-03-23 2002-11-28 Merril Carl R. Methods for treating nuerodegenerative diseases including alzheimer's

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106290852A (en) * 2015-05-11 2017-01-04 宁波大学 Can the AIDS diagnosis device of Dual Drive CGCM of rapid disassembling
CN106290869A (en) * 2015-05-11 2017-01-04 宁波大学 The controller used in syphilis diagnosis micro fluidic device of the Dual Drive CGCM of easily-disassembled framework
CN106290851A (en) * 2015-05-11 2017-01-04 宁波大学 A kind of easily-disassembled cholera diagnosis micro fluidic device of Dual Drive CGCM
CN106290929A (en) * 2015-05-11 2017-01-04 宁波大学 The hypotype swine flue detection device of the Dual Drive CGCM that can disassemble rapidly
CN106290853A (en) * 2015-05-11 2017-01-04 宁波大学 Take the AIDS diagnosis device of the Dual Drive CGCM of easily-disassembled framework

Also Published As

Publication number Publication date
WO2007120166A2 (en) 2007-10-25

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