WO2007120192A3 - Methods and compositions for labeling nucleic acids - Google Patents

Methods and compositions for labeling nucleic acids Download PDF

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Publication number
WO2007120192A3
WO2007120192A3 PCT/US2006/042287 US2006042287W WO2007120192A3 WO 2007120192 A3 WO2007120192 A3 WO 2007120192A3 US 2006042287 W US2006042287 W US 2006042287W WO 2007120192 A3 WO2007120192 A3 WO 2007120192A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
nucleic acid
cellular proliferation
labeling
compositions
Prior art date
Application number
PCT/US2006/042287
Other languages
French (fr)
Other versions
WO2007120192A2 (en
Inventor
Adrian Salic
Timothy J Mitchison
Original Assignee
Harvard College
Adrian Salic
Timothy J Mitchison
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=37968573&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=WO2007120192(A3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Harvard College, Adrian Salic, Timothy J Mitchison filed Critical Harvard College
Priority to EP06851143.5A priority Critical patent/EP1937850B1/en
Publication of WO2007120192A2 publication Critical patent/WO2007120192A2/en
Publication of WO2007120192A3 publication Critical patent/WO2007120192A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5011—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing antineoplastic activity
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label

Abstract

The present invention relates to methods for the labeling of nucleic acid polymers in vitro and in vivo. In particular, the methods include a [3+2] cycloaddition between a nucleotide analogue incorporated into a nucleic acid polymer and a reagent attached to a label. Such methods do not require fixation and denaturation and therefore can be applied to the labeling of nucleic acid polymers in living cells and in organisms. Also provided are methods for measuring cellular proliferation. In these methods, the amount of label incorporated into the DNA is measured as an indication of cellular proliferation. The methods of the invention can be used in a wide variety of applications including clinical diagnosis of diseases and disorders in which cellular proliferation is involved, toxicity assays, and as a tool for the study of chromosomes' ultrastructures.
PCT/US2006/042287 2005-10-27 2006-10-27 Methods and compositions for labeling nucleic acids WO2007120192A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP06851143.5A EP1937850B1 (en) 2005-10-27 2006-10-27 Methods and compositions for labeling nucleic acids

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US73074505P 2005-10-27 2005-10-27
US60/730,745 2005-10-27

Publications (2)

Publication Number Publication Date
WO2007120192A2 WO2007120192A2 (en) 2007-10-25
WO2007120192A3 true WO2007120192A3 (en) 2008-01-17

Family

ID=37968573

Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/US2006/041885 WO2007050811A2 (en) 2005-10-27 2006-10-27 Methods and compositions for labeling nucleic acids
PCT/US2006/042287 WO2007120192A2 (en) 2005-10-27 2006-10-27 Methods and compositions for labeling nucleic acids

Family Applications Before (1)

Application Number Title Priority Date Filing Date
PCT/US2006/041885 WO2007050811A2 (en) 2005-10-27 2006-10-27 Methods and compositions for labeling nucleic acids

Country Status (4)

Country Link
US (8) US7910335B2 (en)
EP (3) EP1937849B1 (en)
JP (2) JP5430937B2 (en)
WO (2) WO2007050811A2 (en)

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Also Published As

Publication number Publication date
US20140065605A1 (en) 2014-03-06
US20140295414A1 (en) 2014-10-02
EP1937850A4 (en) 2013-03-13
JP2009513137A (en) 2009-04-02
US7910335B2 (en) 2011-03-22
US9790541B2 (en) 2017-10-17
US10550422B2 (en) 2020-02-04
US20110065907A1 (en) 2011-03-17
US8541570B2 (en) 2013-09-24
EP1937849A2 (en) 2008-07-02
EP1937850A2 (en) 2008-07-02
WO2007050811A3 (en) 2007-06-21
EP3591069A3 (en) 2020-03-04
WO2007120192A2 (en) 2007-10-25
US20100311063A1 (en) 2010-12-09
US20180087091A1 (en) 2018-03-29
JP5430937B2 (en) 2014-03-05
US20170137866A1 (en) 2017-05-18
WO2007050811A2 (en) 2007-05-03
US20070099222A1 (en) 2007-05-03
EP1937849A4 (en) 2013-03-13
EP1937849B1 (en) 2019-05-29
EP1937850B1 (en) 2019-05-29
EP3591069A2 (en) 2020-01-08
US20070207476A1 (en) 2007-09-06
US9512465B2 (en) 2016-12-06
US7767421B2 (en) 2010-08-03
JP2013027406A (en) 2013-02-07
US8859753B2 (en) 2014-10-14

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