WO2007133831A3 - High throughput genome sequencing on dna arrays - Google Patents

High throughput genome sequencing on dna arrays Download PDF

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Publication number
WO2007133831A3
WO2007133831A3 PCT/US2007/062825 US2007062825W WO2007133831A3 WO 2007133831 A3 WO2007133831 A3 WO 2007133831A3 US 2007062825 W US2007062825 W US 2007062825W WO 2007133831 A3 WO2007133831 A3 WO 2007133831A3
Authority
WO
WIPO (PCT)
Prior art keywords
adaptors
target
sequencing
sequences
methods
Prior art date
Application number
PCT/US2007/062825
Other languages
French (fr)
Other versions
WO2007133831A2 (en
Inventor
Radoje Drmanac
Matthew J Callow
Snezana Drmanac
Original Assignee
Complete Genomics Inc
Radoje Drmanac
Matthew J Callow
Snezana Drmanac
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Complete Genomics Inc, Radoje Drmanac, Matthew J Callow, Snezana Drmanac filed Critical Complete Genomics Inc
Priority to JP2008556583A priority Critical patent/JP5180845B2/en
Priority to EP07757499A priority patent/EP1994180A4/en
Priority to AU2007249635A priority patent/AU2007249635B2/en
Priority to CA002643700A priority patent/CA2643700A1/en
Priority to CN2007800147466A priority patent/CN101432439B/en
Publication of WO2007133831A2 publication Critical patent/WO2007133831A2/en
Publication of WO2007133831A3 publication Critical patent/WO2007133831A3/en
Priority to IL193599A priority patent/IL193599A/en
Priority to IL238895A priority patent/IL238895B/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation

Abstract

The present invention is directed to methods and compositions for acquiring nucleotide sequence information of target sequences using adaptors interspersed in target polynucleotides. The sequence information can be new. e.g. sequencing unknown nucleic acids, re-sequencing, or genotyping. The invention preferably includes methods for inserting a plurality of adaptors at spaced locations within a target polynucleotide or a fragment of a polynucleotide. Such adaptors may serve as platforms for interrogating adjacent sequences using various sequencing chemistries, such as those that identify nucleotides by primer extension, probe ligation, and the like. Encompassed in the invention are methods and compositions for the insertion of known adaptor sequences into target sequences, such that there is an interruption of contiguous target sequence with the adaptors. By sequencing both 'upstream' and 'downstream' of the adaptors, identification of entire target sequences may be accomplished.
PCT/US2007/062825 2005-10-07 2007-02-26 High throughput genome sequencing on dna arrays WO2007133831A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
JP2008556583A JP5180845B2 (en) 2006-02-24 2007-02-26 High-throughput genomic sequencing on DNA arrays
EP07757499A EP1994180A4 (en) 2006-02-24 2007-02-26 High throughput genome sequencing on dna arrays
AU2007249635A AU2007249635B2 (en) 2005-10-07 2007-02-26 High throughput genome sequencing on DNA arrays
CA002643700A CA2643700A1 (en) 2006-02-24 2007-02-26 High throughput genome sequencing on dna arrays
CN2007800147466A CN101432439B (en) 2006-02-24 2007-02-26 High throughput genome sequencing on DNA arrays
IL193599A IL193599A (en) 2006-02-24 2008-08-21 Method and system for determining sequence information for a target polynucleotide
IL238895A IL238895B (en) 2006-02-24 2015-05-19 High throughput genome sequencing on dna arrays

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US77641506P 2006-02-24 2006-02-24
US60/776,415 2006-02-24

Publications (2)

Publication Number Publication Date
WO2007133831A2 WO2007133831A2 (en) 2007-11-22
WO2007133831A3 true WO2007133831A3 (en) 2008-02-07

Family

ID=38694571

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/062825 WO2007133831A2 (en) 2005-10-07 2007-02-26 High throughput genome sequencing on dna arrays

Country Status (8)

Country Link
US (1) US8722326B2 (en)
EP (2) EP2495337A1 (en)
JP (2) JP5180845B2 (en)
CN (1) CN101432439B (en)
AU (1) AU2007249635B2 (en)
CA (1) CA2643700A1 (en)
IL (2) IL193599A (en)
WO (1) WO2007133831A2 (en)

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