WO2007136874A3 - Genomic library construction - Google Patents

Genomic library construction Download PDF

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Publication number
WO2007136874A3
WO2007136874A3 PCT/US2007/012229 US2007012229W WO2007136874A3 WO 2007136874 A3 WO2007136874 A3 WO 2007136874A3 US 2007012229 W US2007012229 W US 2007012229W WO 2007136874 A3 WO2007136874 A3 WO 2007136874A3
Authority
WO
WIPO (PCT)
Prior art keywords
genomic library
library construction
compositions
methods
single cell
Prior art date
Application number
PCT/US2007/012229
Other languages
French (fr)
Other versions
WO2007136874A2 (en
Inventor
George M Church
Kun Zhang
Original Assignee
Harvard College
George M Church
Kun Zhang
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Harvard College, George M Church, Kun Zhang filed Critical Harvard College
Publication of WO2007136874A2 publication Critical patent/WO2007136874A2/en
Priority to US12/265,184 priority Critical patent/US7985546B2/en
Publication of WO2007136874A3 publication Critical patent/WO2007136874A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Abstract

Compositions and methods for amplifying nucleic acid sequences from a single cell are provided. Compositions and methods for constructing a genomic library from a single cell are also provided.
PCT/US2007/012229 2006-05-18 2007-05-18 Genomic library construction WO2007136874A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US12/265,184 US7985546B2 (en) 2006-05-18 2008-11-05 Genomic library construction

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US80134006P 2006-05-18 2006-05-18
US60/801,340 2006-05-18

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US12/265,184 Continuation US7985546B2 (en) 2006-05-18 2008-11-05 Genomic library construction

Publications (2)

Publication Number Publication Date
WO2007136874A2 WO2007136874A2 (en) 2007-11-29
WO2007136874A3 true WO2007136874A3 (en) 2008-11-27

Family

ID=38723917

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/012229 WO2007136874A2 (en) 2006-05-18 2007-05-18 Genomic library construction

Country Status (2)

Country Link
US (1) US7985546B2 (en)
WO (1) WO2007136874A2 (en)

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US9411930B2 (en) 2013-02-01 2016-08-09 The Regents Of The University Of California Methods for genome assembly and haplotype phasing
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WO2016036553A1 (en) * 2014-09-05 2016-03-10 University Of Florida Research Foundation, Inc. Multiplexed pcr assay for high throughput genotyping
WO2016134034A1 (en) 2015-02-17 2016-08-25 Dovetail Genomics Llc Nucleic acid sequence assembly
US10697010B2 (en) 2015-02-19 2020-06-30 Becton, Dickinson And Company High-throughput single-cell analysis combining proteomic and genomic information
WO2016138496A1 (en) 2015-02-27 2016-09-01 Cellular Research, Inc. Spatially addressable molecular barcoding
US11807896B2 (en) 2015-03-26 2023-11-07 Dovetail Genomics, Llc Physical linkage preservation in DNA storage
WO2016160844A2 (en) 2015-03-30 2016-10-06 Cellular Research, Inc. Methods and compositions for combinatorial barcoding
WO2016172373A1 (en) 2015-04-23 2016-10-27 Cellular Research, Inc. Methods and compositions for whole transcriptome amplification
US11124823B2 (en) 2015-06-01 2021-09-21 Becton, Dickinson And Company Methods for RNA quantification
ES2745694T3 (en) 2015-09-11 2020-03-03 Cellular Res Inc Methods and compositions for nucleic acid library normalization
CN108368542B (en) 2015-10-19 2022-04-08 多弗泰尔基因组学有限责任公司 Methods for genome assembly, haplotype phasing, and target-independent nucleic acid detection
EP3420108A4 (en) 2016-02-23 2019-11-06 Dovetail Genomics LLC Generation of phased read-sets for genome assembly and haplotype phasing
ES2956757T3 (en) 2016-05-02 2023-12-27 Becton Dickinson Co Accurate molecular barcode coding
IL262946B2 (en) 2016-05-13 2023-03-01 Dovetail Genomics Llc Recovering long-range linkage information from preserved samples
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
US11397882B2 (en) 2016-05-26 2022-07-26 Becton, Dickinson And Company Molecular label counting adjustment methods
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
KR102363716B1 (en) 2016-09-26 2022-02-18 셀룰러 리서치, 인크. Determination of protein expression using reagents having barcoded oligonucleotide sequences
EP3539035B1 (en) 2016-11-08 2024-04-17 Becton, Dickinson and Company Methods for expression profile classification
SG11201903158RA (en) 2016-11-08 2019-05-30 Cellular Res Inc Methods for cell label classification
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US11319583B2 (en) 2017-02-01 2022-05-03 Becton, Dickinson And Company Selective amplification using blocking oligonucleotides
JP2020522262A (en) 2017-06-05 2020-07-30 ベクトン・ディキンソン・アンド・カンパニーＢｅｃｔｏｎ， Ｄｉｃｋｉｎｓｏｎ Ａｎｄ Ｃｏｍｐａｎｙ Sample index addition for single cells
CN109811045B (en) * 2017-11-22 2022-05-31 深圳华大智造科技股份有限公司 Construction method and application of high-throughput single-cell full-length transcriptome sequencing library
EP3728636A1 (en) 2017-12-19 2020-10-28 Becton, Dickinson and Company Particles associated with oligonucleotides
EP4234717A3 (en) 2018-05-03 2023-11-01 Becton, Dickinson and Company High throughput multiomics sample analysis
CN112243461A (en) 2018-05-03 2021-01-19 贝克顿迪金森公司 Molecular barcoding at opposite transcript ends
CN112805389A (en) 2018-10-01 2021-05-14 贝克顿迪金森公司 Determination of 5' transcript sequences
WO2020097315A1 (en) 2018-11-08 2020-05-14 Cellular Research, Inc. Whole transcriptome analysis of single cells using random priming
US11492660B2 (en) 2018-12-13 2022-11-08 Becton, Dickinson And Company Selective extension in single cell whole transcriptome analysis
US11371076B2 (en) 2019-01-16 2022-06-28 Becton, Dickinson And Company Polymerase chain reaction normalization through primer titration
EP3914728B1 (en) 2019-01-23 2023-04-05 Becton, Dickinson and Company Oligonucleotides associated with antibodies
EP4004231A1 (en) 2019-07-22 2022-06-01 Becton, Dickinson and Company Single cell chromatin immunoprecipitation sequencing assay
US11773436B2 (en) 2019-11-08 2023-10-03 Becton, Dickinson And Company Using random priming to obtain full-length V(D)J information for immune repertoire sequencing
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US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
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US6617137B2 (en) * 2001-10-15 2003-09-09 Molecular Staging Inc. Method of amplifying whole genomes without subjecting the genome to denaturing conditions

Also Published As

Publication number Publication date
US20090137407A1 (en) 2009-05-28
US7985546B2 (en) 2011-07-26
WO2007136874A2 (en) 2007-11-29

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