WO2007138568A3 - A device for microscopy flow-through experiments on non-adherent live cells - Google Patents

A device for microscopy flow-through experiments on non-adherent live cells Download PDF

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Publication number
WO2007138568A3
WO2007138568A3 PCT/IL2007/000570 IL2007000570W WO2007138568A3 WO 2007138568 A3 WO2007138568 A3 WO 2007138568A3 IL 2007000570 W IL2007000570 W IL 2007000570W WO 2007138568 A3 WO2007138568 A3 WO 2007138568A3
Authority
WO
WIPO (PCT)
Prior art keywords
experiments
interest
flow
cells
live cells
Prior art date
Application number
PCT/IL2007/000570
Other languages
French (fr)
Other versions
WO2007138568A2 (en
Inventor
Cher Ashtamker
Robert Fluhr
Original Assignee
Yeda Res & Dev
Cher Ashtamker
Robert Fluhr
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Yeda Res & Dev, Cher Ashtamker, Robert Fluhr filed Critical Yeda Res & Dev
Publication of WO2007138568A2 publication Critical patent/WO2007138568A2/en
Publication of WO2007138568A3 publication Critical patent/WO2007138568A3/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/34—Microscope slides, e.g. mounting specimens on microscope slides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0822—Slides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0877—Flow chambers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/08—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis

Abstract

A device used in microscopic applications and particularly to those used for examining specimens containing non-adherent live cells or other particles of interest suspended in a liquid medium, in flow-through experiments, includes an open mesh to be located between a slide plate and coverslip for receiving a specimen of the cells of interest. The open mesh is constructed and dimensioned to make up a multitude of small mesh openings defining a two-dimensional array of interconnecting physical traps for receiving the specimen and is effective in restricting the movement of the cells of interest therein without unduly affecting the viability of the cells of interest, or restricting the flow of the surrounding medium, thus allowing the conducting of flow-through experiments.
PCT/IL2007/000570 2006-05-25 2007-05-10 A device for microscopy flow-through experiments on non-adherent live cells WO2007138568A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US80811106P 2006-05-25 2006-05-25
US60/808,111 2006-05-25

Publications (2)

Publication Number Publication Date
WO2007138568A2 WO2007138568A2 (en) 2007-12-06
WO2007138568A3 true WO2007138568A3 (en) 2009-04-23

Family

ID=38779079

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IL2007/000570 WO2007138568A2 (en) 2006-05-25 2007-05-10 A device for microscopy flow-through experiments on non-adherent live cells

Country Status (1)

Country Link
WO (1) WO2007138568A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201121959D0 (en) * 2011-12-21 2012-02-01 Univ Leeds Assay of functional cell viability
CN103105670A (en) * 2013-02-06 2013-05-15 李宏 Cell slide glass for microscopic observation
KR20170099737A (en) * 2016-02-23 2017-09-01 노을 주식회사 Contact-type staining patch and staining method using the same
CN109351377A (en) * 2018-11-28 2019-02-19 浙江警察学院 The micro-fluidic chip of impurity can unidirectionally be intercepted by setting crossed array in a kind of

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3768914A (en) * 1972-09-18 1973-10-30 T Kinney Electron microscopy tissue grid staining and storing rack and method
US5257128A (en) * 1988-06-22 1993-10-26 Board Of Regents, The University Of Texas System Freezing/perfusion microscope stage
US5792653A (en) * 1991-09-28 1998-08-11 Kernforschungszenlrum Karlsruhe Gmbh Substrate for cell structures

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3768914A (en) * 1972-09-18 1973-10-30 T Kinney Electron microscopy tissue grid staining and storing rack and method
US5257128A (en) * 1988-06-22 1993-10-26 Board Of Regents, The University Of Texas System Freezing/perfusion microscope stage
US5792653A (en) * 1991-09-28 1998-08-11 Kernforschungszenlrum Karlsruhe Gmbh Substrate for cell structures

Also Published As

Publication number Publication date
WO2007138568A2 (en) 2007-12-06

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