WO2008005998A3 - Centimeter-scale, integrated diagnostics incubator for biological culturing - Google Patents

Centimeter-scale, integrated diagnostics incubator for biological culturing Download PDF

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Publication number
WO2008005998A3
WO2008005998A3 PCT/US2007/072777 US2007072777W WO2008005998A3 WO 2008005998 A3 WO2008005998 A3 WO 2008005998A3 US 2007072777 W US2007072777 W US 2007072777W WO 2008005998 A3 WO2008005998 A3 WO 2008005998A3
Authority
WO
WIPO (PCT)
Prior art keywords
chamber
incubators
enclosure
disposed
culturing
Prior art date
Application number
PCT/US2007/072777
Other languages
French (fr)
Other versions
WO2008005998A2 (en
Inventor
Jelena Vukasinovic
Ari Glezer
Original Assignee
Georgia Tech Res Inst
Jelena Vukasinovic
Ari Glezer
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US11/483,126 external-priority patent/US7855070B2/en
Application filed by Georgia Tech Res Inst, Jelena Vukasinovic, Ari Glezer filed Critical Georgia Tech Res Inst
Priority to US12/529,881 priority Critical patent/US20100151571A1/en
Publication of WO2008005998A2 publication Critical patent/WO2008005998A2/en
Publication of WO2008005998A3 publication Critical patent/WO2008005998A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/22—Transparent or translucent parts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10—Perfusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L1/00—Enclosures; Chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip

Abstract

Disclosed are portable, disposable, centimeter-scale, integrated diagnostics incubators for use in biological culturing. An exemplary incubator comprises an optically accessible enclosure having a plurality of fluidic ports. A heating element is disposed within the enclosure that is coupled to an external heater controller. An autoclavable microfluidic perfusion chamber is disposed within the enclosure that comprises a cell culture life support chamber, an inlet port disposed in the perfusion chamber, a collection chamber in communication with the culture chamber, an outlet port coupled to the collection pool, and a perfusing substrate. An optically transparent, gas permeable membrane is attachable to the top of the perfusion chamber. The incubators have optical accessibility, forced flow fluidic control, temperature control, are portable and modular, and are inexpensively manufactured. The incubators permit in-the- field drug testing and culturing of biological tissues.
PCT/US2007/072777 2006-07-07 2007-07-03 Centimeter-scale, integrated diagnostics incubator for biological culturing WO2008005998A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US12/529,881 US20100151571A1 (en) 2006-07-07 2007-07-03 Centimeter-scale, integrated diagnostics incubator for biological culturing

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US11/483,126 US7855070B2 (en) 2005-07-08 2006-07-07 Centimeter-scale, integrated diagnostics incubator for biological culturing
US11/483,126 2006-07-07
US85122206P 2006-10-12 2006-10-12
US60/851,222 2006-10-12

Publications (2)

Publication Number Publication Date
WO2008005998A2 WO2008005998A2 (en) 2008-01-10
WO2008005998A3 true WO2008005998A3 (en) 2008-10-09

Family

ID=38895452

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/072777 WO2008005998A2 (en) 2006-07-07 2007-07-03 Centimeter-scale, integrated diagnostics incubator for biological culturing

Country Status (1)

Country Link
WO (1) WO2008005998A2 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3252139A1 (en) 2001-09-06 2017-12-06 Rapid Micro Biosystems, Inc. Rapid detection of replicating cells
JP5160428B2 (en) 2005-09-26 2013-03-13 ラピッド マイクロ バイオシステムズ インコーポレイテッド Cassette containing growth medium
WO2010024779A1 (en) * 2008-08-27 2010-03-04 Agency For Science, Technology And Research Microfluidic continuous flow device for culturing biological material
US10384203B2 (en) 2008-09-24 2019-08-20 First Light Biosciences, Inc. Kits and devices for detecting analytes
DE102008056037B4 (en) * 2008-11-05 2012-10-31 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Microfluidic bioreactor
GB0820779D0 (en) 2008-11-13 2008-12-17 Artelis S A Cell culture device and method of culturing cells
WO2012033439A1 (en) * 2010-09-10 2012-03-15 Gradientech Ab Microfluidic capsule
MX368123B (en) 2011-11-07 2019-09-19 Rapid Micro Biosystems Inc Cassette for sterility testing.
MX2014012439A (en) * 2012-04-16 2015-01-16 Rapid Micro Biosystems Inc Cell culturing device.
CN110108622B (en) * 2019-06-17 2024-02-02 辽宁中医药大学 Isolated microvascular endothelial barrier function detection method and device
CN111205980B (en) * 2020-01-20 2024-01-26 河海大学常州校区 Cell culture device for single cell in-situ real-time microscopic observation and operation method thereof

Citations (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5879876A (en) * 1997-03-24 1999-03-09 Lifenet Continuous-multi-step dilution process and apparatus, for the removal of cryoprotectants from cryopreserved tissues
US6057150A (en) * 1997-09-19 2000-05-02 Bio-Rad Laboratories, Inc. Biaxial strain system for cultured cells
US6197575B1 (en) * 1998-03-18 2001-03-06 Massachusetts Institute Of Technology Vascularized perfused microtissue/micro-organ arrays
US6368838B1 (en) * 1993-10-04 2002-04-09 President And Fellows Of Havard College Adhering cells to cytophilic islands separated by cytophobic regions to form patterns and manipulate cells
US20030004394A1 (en) * 2001-03-13 2003-01-02 Carpay Wilhelmus Marinus Incubator system provided with a temperature control system
US6503751B2 (en) * 1996-02-09 2003-01-07 Thermo Forma Inc. Controlled atmosphere incubator
US20040132175A1 (en) * 2000-07-19 2004-07-08 Jerome Vetillard Cell culture chamber and bioreactor for extracorporeal culture of animal cells
US20050089993A1 (en) * 2002-05-01 2005-04-28 Paolo Boccazzi Apparatus and methods for simultaneous operation of miniaturized reactors
US20050112759A1 (en) * 2003-06-20 2005-05-26 Milica Radisic Application of electrical stimulation for functional tissue engineering in vitro and in vivo
US20050186671A1 (en) * 2000-10-02 2005-08-25 Cannon Thomas F. Automated bioculture and bioculture experiments system
US20050221269A1 (en) * 2004-04-05 2005-10-06 Organ Recovery Systems Apparatus and method for perfusing an organ or tissue for isolating cells from the organ or tissue
US20060073539A1 (en) * 2001-08-06 2006-04-06 Wikswo John P Apparatus and methods for using biological material to discriminate an agent

Patent Citations (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6368838B1 (en) * 1993-10-04 2002-04-09 President And Fellows Of Havard College Adhering cells to cytophilic islands separated by cytophobic regions to form patterns and manipulate cells
US6503751B2 (en) * 1996-02-09 2003-01-07 Thermo Forma Inc. Controlled atmosphere incubator
US5879876A (en) * 1997-03-24 1999-03-09 Lifenet Continuous-multi-step dilution process and apparatus, for the removal of cryoprotectants from cryopreserved tissues
US6057150A (en) * 1997-09-19 2000-05-02 Bio-Rad Laboratories, Inc. Biaxial strain system for cultured cells
US6197575B1 (en) * 1998-03-18 2001-03-06 Massachusetts Institute Of Technology Vascularized perfused microtissue/micro-organ arrays
US20040132175A1 (en) * 2000-07-19 2004-07-08 Jerome Vetillard Cell culture chamber and bioreactor for extracorporeal culture of animal cells
US20050186671A1 (en) * 2000-10-02 2005-08-25 Cannon Thomas F. Automated bioculture and bioculture experiments system
US20030004394A1 (en) * 2001-03-13 2003-01-02 Carpay Wilhelmus Marinus Incubator system provided with a temperature control system
US20060073539A1 (en) * 2001-08-06 2006-04-06 Wikswo John P Apparatus and methods for using biological material to discriminate an agent
US20050089993A1 (en) * 2002-05-01 2005-04-28 Paolo Boccazzi Apparatus and methods for simultaneous operation of miniaturized reactors
US20050112759A1 (en) * 2003-06-20 2005-05-26 Milica Radisic Application of electrical stimulation for functional tissue engineering in vitro and in vivo
US20050221269A1 (en) * 2004-04-05 2005-10-06 Organ Recovery Systems Apparatus and method for perfusing an organ or tissue for isolating cells from the organ or tissue

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Publication number Publication date
WO2008005998A2 (en) 2008-01-10

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