WO2008070352A3 - Efficient arrays of amplified polynucleotides - Google Patents

Efficient arrays of amplified polynucleotides Download PDF

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Publication number
WO2008070352A3
WO2008070352A3 PCT/US2007/082896 US2007082896W WO2008070352A3 WO 2008070352 A3 WO2008070352 A3 WO 2008070352A3 US 2007082896 W US2007082896 W US 2007082896W WO 2008070352 A3 WO2008070352 A3 WO 2008070352A3
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WO
WIPO (PCT)
Prior art keywords
polynucleotides
amplified polynucleotides
arrays
analysis
efficient arrays
Prior art date
Application number
PCT/US2007/082896
Other languages
French (fr)
Other versions
WO2008070352A2 (en
Inventor
Radoje Drmanac
Matthew Callow
Original Assignee
Complete Genomics Inc
Radoje Drmanac
Matthew Callow
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Complete Genomics Inc, Radoje Drmanac, Matthew Callow filed Critical Complete Genomics Inc
Publication of WO2008070352A2 publication Critical patent/WO2008070352A2/en
Publication of WO2008070352A3 publication Critical patent/WO2008070352A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation

Abstract

The present invention is related generally to analysis of polynucleotides, particularly polynucleotides derived from genomic DNA. The invention provides methods, compositions and systems for such analysis. Encompassed by the invention are arrays of polynucleotides in which the polynucleotides have undergone multiple rounds of amplification in order to increase the strength of signals associated with single polynucleotide molecules.
PCT/US2007/082896 2006-10-27 2007-10-29 Efficient arrays of amplified polynucleotides WO2008070352A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US86315706P 2006-10-27 2006-10-27
US60/863,157 2006-10-27

Publications (2)

Publication Number Publication Date
WO2008070352A2 WO2008070352A2 (en) 2008-06-12
WO2008070352A3 true WO2008070352A3 (en) 2008-10-30

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PCT/US2007/082896 WO2008070352A2 (en) 2006-10-27 2007-10-29 Efficient arrays of amplified polynucleotides

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US (6) US7910302B2 (en)
WO (1) WO2008070352A2 (en)

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