WO2008087642A3 - Nucleic acid constructs and methods for specific silencing of h19 - Google Patents

Nucleic acid constructs and methods for specific silencing of h19 Download PDF

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Publication number
WO2008087642A3
WO2008087642A3 PCT/IL2008/000072 IL2008000072W WO2008087642A3 WO 2008087642 A3 WO2008087642 A3 WO 2008087642A3 IL 2008000072 W IL2008000072 W IL 2008000072W WO 2008087642 A3 WO2008087642 A3 WO 2008087642A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
methods
acid constructs
specific silencing
constructs
Prior art date
Application number
PCT/IL2008/000072
Other languages
French (fr)
Other versions
WO2008087642A2 (en
Inventor
Abraham Hochberg
Original Assignee
Yissum Res Dev Co
Abraham Hochberg
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Yissum Res Dev Co, Abraham Hochberg filed Critical Yissum Res Dev Co
Priority to US12/523,298 priority Critical patent/US20100086526A1/en
Priority to EP08702653A priority patent/EP2111450A2/en
Priority to CA002675967A priority patent/CA2675967A1/en
Publication of WO2008087642A2 publication Critical patent/WO2008087642A2/en
Publication of WO2008087642A3 publication Critical patent/WO2008087642A3/en
Priority to IL199867A priority patent/IL199867A0/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A61P19/02—Drugs for skeletal disorders for joint disorders, e.g. arthritis, arthrosis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/06—Immunosuppressants, e.g. drugs for graft rejection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.

Abstract

The present invention is directed to recombinant constructs and methods for treating pathological conditions associated with H19 expression, such as tumors characterized by up-regulated expression of H19 RNA. Specifically, the recombinant constructs of the invention comprise at least one nucleic acid sequence encoding a small interfering RNA (siRNA) molecule directed to H19, the nucleic acid sequence being operably linked to at least one H19-specific transcription-regulating sequence. Vectors comprising these constructs, pharmaceutical compositions comprising them and therapeutic methods of using same are also provided.
PCT/IL2008/000072 2007-01-16 2008-01-16 Nucleic acid constructs and methods for specific silencing of h19 WO2008087642A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
US12/523,298 US20100086526A1 (en) 2007-01-16 2008-01-16 Nucleic acid constructs and methods for specific silencing of h19
EP08702653A EP2111450A2 (en) 2007-01-16 2008-01-16 Nucleic acid constructs and methods for specific silencing of h19
CA002675967A CA2675967A1 (en) 2007-01-16 2008-01-16 Nucleic acid constructs and methods for specific silencing of h19
IL199867A IL199867A0 (en) 2007-01-16 2009-07-14 H19 silencing nucleic acid agents for treating rheumatoid arthritis

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US88042507P 2007-01-16 2007-01-16
US88043007P 2007-01-16 2007-01-16
US60/880,425 2007-01-16
US60/880,430 2007-01-16

Publications (2)

Publication Number Publication Date
WO2008087642A2 WO2008087642A2 (en) 2008-07-24
WO2008087642A3 true WO2008087642A3 (en) 2008-09-04

Family

ID=39434171

Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/IL2008/000071 WO2008087641A2 (en) 2007-01-16 2008-01-16 H19 silencing nucleic acid agents for treating rheumatoid arthritis
PCT/IL2008/000072 WO2008087642A2 (en) 2007-01-16 2008-01-16 Nucleic acid constructs and methods for specific silencing of h19

Family Applications Before (1)

Application Number Title Priority Date Filing Date
PCT/IL2008/000071 WO2008087641A2 (en) 2007-01-16 2008-01-16 H19 silencing nucleic acid agents for treating rheumatoid arthritis

Country Status (5)

Country Link
US (2) US20100086526A1 (en)
EP (2) EP2111450A2 (en)
AT (1) ATE548454T1 (en)
CA (2) CA2675967A1 (en)
WO (2) WO2008087641A2 (en)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
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CA2614531C (en) * 2005-07-07 2015-06-16 Avraham Hochberg Nucleic acid agents for downregulating h19, and methods of using same
HUE037173T2 (en) 2006-08-08 2018-08-28 Univ Bonn Rheinische Friedrich Wilhelms Structure and use of 5' phosphate oligonucleotides
WO2008087641A2 (en) * 2007-01-16 2008-07-24 Yissum Research Development Company Of The Hebrew University Of Jerusalem H19 silencing nucleic acid agents for treating rheumatoid arthritis
US20100196403A1 (en) * 2007-01-29 2010-08-05 Jacob Hochman Antibody conjugates for circumventing multi-drug resistance
EP2203187B1 (en) 2007-10-25 2012-08-29 Yissum Research Development Company of the Hebrew University of Jerusalem, Ltd. Constructs containing multiple expression cassettes for cancer therapy
EP2297323A1 (en) 2008-05-21 2011-03-23 Hartmann, Gunther 5' triphosphate oligonucleotide with blunt end and uses thereof
JP2012515532A (en) 2009-01-20 2012-07-12 ラモット アット テル アビブ ユニバーシティ， リミテッド MIR-21 promoter driven target cancer treatment
DE102010056610A1 (en) * 2010-12-31 2012-07-05 Volker A. Erdmann Pharmaceutical composition containing L-DNA
EP2508530A1 (en) 2011-03-28 2012-10-10 Rheinische Friedrich-Wilhelms-Universität Bonn Purification of triphosphorylated oligonucleotides using capture tags
EP2712870A1 (en) 2012-09-27 2014-04-02 Rheinische Friedrich-Wilhelms-Universität Bonn Novel RIG-I ligands and methods for producing them
WO2016162807A1 (en) * 2015-04-10 2016-10-13 Universita' Degli Studi Di Palermo Microrna inhibitor for the treatment of aberrant angiogenesis and pathologies accompanied thereby
BR112017023788A2 (en) 2015-05-05 2018-10-16 Yissum Res Dev Co Of Hebrew Univ Jerusalem Ltd nucleic acid - cationic polymer compositions and process for preparing said composition
WO2020206055A1 (en) * 2019-04-02 2020-10-08 Yale University Methods of treatment of diseases and disorders associated with increased tet level, increased h19 level, increased level of tgf signaling, or any combination thereof

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Also Published As

Publication number Publication date
ATE548454T1 (en) 2012-03-15
WO2008087642A2 (en) 2008-07-24
US20100105759A1 (en) 2010-04-29
CA2675964A1 (en) 2008-07-24
EP2111449B1 (en) 2012-03-07
EP2111450A2 (en) 2009-10-28
WO2008087641A2 (en) 2008-07-24
CA2675964C (en) 2015-12-22
CA2675967A1 (en) 2008-07-24
US7928083B2 (en) 2011-04-19
WO2008087641A3 (en) 2008-09-04
EP2111449A2 (en) 2009-10-28
US20100086526A1 (en) 2010-04-08

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