WO2008108843A3 - Methods and kits for amplifying dna - Google Patents

Methods and kits for amplifying dna Download PDF

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Publication number
WO2008108843A3
WO2008108843A3 PCT/US2007/063103 US2007063103W WO2008108843A3 WO 2008108843 A3 WO2008108843 A3 WO 2008108843A3 US 2007063103 W US2007063103 W US 2007063103W WO 2008108843 A3 WO2008108843 A3 WO 2008108843A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
products
disclosed
nucleic acid
appearance
Prior art date
Application number
PCT/US2007/063103
Other languages
French (fr)
Other versions
WO2008108843A2 (en
Inventor
Michael M Becker
Wai-Chung Lam
Kristin W Livezey
Steven T Brentano
Daniel P Kolk
Astrid R W Schroder
Original Assignee
Gen Probe Inc
Michael M Becker
Wai-Chung Lam
Kristin W Livezey
Steven T Brentano
Daniel P Kolk
Astrid R W Schroder
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gen Probe Inc, Michael M Becker, Wai-Chung Lam, Kristin W Livezey, Steven T Brentano, Daniel P Kolk, Astrid R W Schroder filed Critical Gen Probe Inc
Priority to JP2009551981A priority Critical patent/JP2010520750A/en
Priority to PCT/US2007/063103 priority patent/WO2008108843A2/en
Priority to AU2007348215A priority patent/AU2007348215A1/en
Priority to EP07757746A priority patent/EP2118305A4/en
Priority to CA002678799A priority patent/CA2678799A1/en
Publication of WO2008108843A2 publication Critical patent/WO2008108843A2/en
Publication of WO2008108843A3 publication Critical patent/WO2008108843A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]

Abstract

Novel methods of synthesizing multiple copies of a target nucleic acid sequence which are autocatalytic are disclosed (/. e., able to cycle automatically without the need to modify reaction conditions such as temperature, pH, or ionic strength and using the product of one cycle in the next one). In particular, methods of nucleic acid amplification are disclosed which are robust and efficient, while reducing the appearance of side-products. In general, the methods use priming oligonucleotides that target only one sense of a target nucleic acid, a promoter oligonucleotide modified to prevent polymerase extension from its 3 '-terminus and, optionally, a means for terminating a primer extension reaction, to amplify RNA or DNA molecules in vitro, while reducing or substantially eliminating the formation of side-products. The disclosed methods minimizes or substantially eliminate the emergence of side-products, thus providing ahigh level of specificity. Furthermore, the appearance of side-products can complicate the analysis of the amplification reaction by various molecular detection techniques. The disclosed methods minimize or substantially eliminate this problem, thus providing enhanced levels of sensitivity.
PCT/US2007/063103 2007-03-01 2007-03-01 Methods and kits for amplifying dna WO2008108843A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
JP2009551981A JP2010520750A (en) 2007-03-01 2007-03-01 Method and kit for amplifying DNA
PCT/US2007/063103 WO2008108843A2 (en) 2007-03-01 2007-03-01 Methods and kits for amplifying dna
AU2007348215A AU2007348215A1 (en) 2007-03-01 2007-03-01 Methods and kits for amplifying DNA
EP07757746A EP2118305A4 (en) 2007-03-01 2007-03-01 Methods and kits for amplifying dna
CA002678799A CA2678799A1 (en) 2007-03-01 2007-03-01 Methods and kits for amplifying dna

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US2007/063103 WO2008108843A2 (en) 2007-03-01 2007-03-01 Methods and kits for amplifying dna

Publications (2)

Publication Number Publication Date
WO2008108843A2 WO2008108843A2 (en) 2008-09-12
WO2008108843A3 true WO2008108843A3 (en) 2008-10-30

Family

ID=39738944

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/063103 WO2008108843A2 (en) 2007-03-01 2007-03-01 Methods and kits for amplifying dna

Country Status (5)

Country Link
EP (1) EP2118305A4 (en)
JP (1) JP2010520750A (en)
AU (1) AU2007348215A1 (en)
CA (1) CA2678799A1 (en)
WO (1) WO2008108843A2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006026388A2 (en) * 2004-08-27 2006-03-09 Gen-Probe Incorporated Single-primer nucleic acid amplification methods
CA2766391C (en) 2009-07-01 2021-04-20 Gen-Probe Incorporated Methods and compositions for multiplex nucleic acid amplification
CN102725424B (en) * 2010-01-25 2014-07-09 Rd生物科技公司 Self-folding amplification of target nucleic acid
JP6464086B2 (en) 2012-08-30 2019-02-06 ジェン−プローブ・インコーポレーテッド Multiphase nucleic acid amplification
US11098303B2 (en) * 2014-07-30 2021-08-24 President And Fellows Of Harvard College Systems and methods for determining nucleic acids
WO2018218150A1 (en) 2017-05-26 2018-11-29 President And Fellows Of Harvard College Systems and methods for high-throughput image-based screening
CN109020974B (en) * 2018-11-02 2021-01-05 哈尔滨商业大学 Synthesis method and application of 5 '-thenoyl ester-3' -deoxyadenosine

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5169766A (en) * 1991-06-14 1992-12-08 Life Technologies, Inc. Amplification of nucleic acid molecules
US5409818A (en) * 1988-02-24 1995-04-25 Cangene Corporation Nucleic acid amplification process
US5554516A (en) * 1992-05-06 1996-09-10 Gen-Probe Incorporated Nucleic acid sequence amplification method, composition and kit
US20030194723A1 (en) * 2001-11-02 2003-10-16 Cunningham Melissa M. Compositions, methods and kits for determining the presence of Mycoplasma pneumoniae in a test sample
US20040175729A1 (en) * 2002-11-08 2004-09-09 Sysmex Corporation Primer for nucleic acid amplification to detect carcinoembryonic antigen and test method using such primer
US20060046265A1 (en) * 2004-08-27 2006-03-02 Gen-Probe Incorporated Single-primer nucleic acid amplification methods
US20070202523A1 (en) * 2004-08-27 2007-08-30 Becker Michael M Methods and kits for amplifying dna
US20070281317A1 (en) * 2006-06-06 2007-12-06 Gen-Probe Incorporated Tagged oligonucleotides and their use in nucleic acid amplification methods

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2708288B1 (en) * 1993-07-26 1995-09-01 Bio Merieux Method for amplification of nucleic acids by transcription using displacement, reagents and necessary for the implementation of this method.

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5409818A (en) * 1988-02-24 1995-04-25 Cangene Corporation Nucleic acid amplification process
US5169766A (en) * 1991-06-14 1992-12-08 Life Technologies, Inc. Amplification of nucleic acid molecules
US5554516A (en) * 1992-05-06 1996-09-10 Gen-Probe Incorporated Nucleic acid sequence amplification method, composition and kit
US20030194723A1 (en) * 2001-11-02 2003-10-16 Cunningham Melissa M. Compositions, methods and kits for determining the presence of Mycoplasma pneumoniae in a test sample
US20040175729A1 (en) * 2002-11-08 2004-09-09 Sysmex Corporation Primer for nucleic acid amplification to detect carcinoembryonic antigen and test method using such primer
US20060046265A1 (en) * 2004-08-27 2006-03-02 Gen-Probe Incorporated Single-primer nucleic acid amplification methods
WO2006026388A2 (en) * 2004-08-27 2006-03-09 Gen-Probe Incorporated Single-primer nucleic acid amplification methods
US20070202523A1 (en) * 2004-08-27 2007-08-30 Becker Michael M Methods and kits for amplifying dna
US20070299254A1 (en) * 2004-08-27 2007-12-27 Gen-Probe Incorporated Kits for Performing Amplification Reactions
US20070281317A1 (en) * 2006-06-06 2007-12-06 Gen-Probe Incorporated Tagged oligonucleotides and their use in nucleic acid amplification methods

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP2118305A4 *

Also Published As

Publication number Publication date
JP2010520750A (en) 2010-06-17
WO2008108843A2 (en) 2008-09-12
AU2007348215A1 (en) 2008-09-12
CA2678799A1 (en) 2008-09-12
EP2118305A2 (en) 2009-11-18
EP2118305A4 (en) 2010-11-10

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