WO2008151023A3 - Methods and compositions for multiple displacement amplification of nucleic acids - Google Patents

Methods and compositions for multiple displacement amplification of nucleic acids Download PDF

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Publication number
WO2008151023A3
WO2008151023A3 PCT/US2008/065332 US2008065332W WO2008151023A3 WO 2008151023 A3 WO2008151023 A3 WO 2008151023A3 US 2008065332 W US2008065332 W US 2008065332W WO 2008151023 A3 WO2008151023 A3 WO 2008151023A3
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WO
WIPO (PCT)
Prior art keywords
displacement amplification
multiple displacement
methods
nucleic acid
primers
Prior art date
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PCT/US2008/065332
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French (fr)
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WO2008151023A2 (en
Inventor
David A Duncan
Mark W Eshoo
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Ibis Biosciences Inc
David A Duncan
Mark W Eshoo
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Ibis Biosciences Inc, David A Duncan, Mark W Eshoo filed Critical Ibis Biosciences Inc
Priority to US12/602,641 priority Critical patent/US9598724B2/en
Publication of WO2008151023A2 publication Critical patent/WO2008151023A2/en
Publication of WO2008151023A3 publication Critical patent/WO2008151023A3/en
Priority to US15/463,846 priority patent/US20170253920A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6846—Common amplification features

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Disclosed are methods for multiple displacement amplification of a nucleic acid sequence in a sample. The nucleic acid is contacted with a reaction mixture that includes a set of oligonucleotide primers and a plurality of polymerase enzymes. The reaction mixture is subjected to conditions under which the nucleic acid sequence is amplified to produce an amplification product in a multiple displacement amplification reaction. Also disclosed are kits containing a set of oligonucleotide primers with random sequences having lengths of 6 to 8 nucleobases. At least some of the individual members of the primers have one or more ribose modifications that stabilize or lock the ribose ring in a 3' -endo conformation. At least some of the primers have one or more universal nucleobases.
PCT/US2008/065332 2007-06-01 2008-05-30 Methods and compositions for multiple displacement amplification of nucleic acids WO2008151023A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US12/602,641 US9598724B2 (en) 2007-06-01 2008-05-30 Methods and compositions for multiple displacement amplification of nucleic acids
US15/463,846 US20170253920A1 (en) 2007-06-01 2017-03-20 Methods and compositions for multiple displacement amplification of nucleic acids

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US94160307P 2007-06-01 2007-06-01
US60/941,603 2007-06-01

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US12/602,641 A-371-Of-International US9598724B2 (en) 2007-06-01 2008-05-30 Methods and compositions for multiple displacement amplification of nucleic acids
US15/463,846 Division US20170253920A1 (en) 2007-06-01 2017-03-20 Methods and compositions for multiple displacement amplification of nucleic acids

Publications (2)

Publication Number Publication Date
WO2008151023A2 WO2008151023A2 (en) 2008-12-11
WO2008151023A3 true WO2008151023A3 (en) 2009-02-19

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US (2) US9598724B2 (en)
WO (1) WO2008151023A2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2006251866B2 (en) * 2005-05-26 2007-11-29 Human Genetic Signatures Pty Ltd Isothermal strand displacement amplification using primers containing a non-regular base
US9598724B2 (en) 2007-06-01 2017-03-21 Ibis Biosciences, Inc. Methods and compositions for multiple displacement amplification of nucleic acids
GB0901593D0 (en) 2009-01-30 2009-03-11 Touchlight Genetics Ltd Production of closed linear DNA
EP2957641B1 (en) 2009-10-15 2017-05-17 Ibis Biosciences, Inc. Multiple displacement amplification
US9428801B2 (en) * 2010-12-29 2016-08-30 Ibis Biosciences, Inc. Rapid whole genome amplification
US20150167065A1 (en) * 2013-12-13 2015-06-18 General Electric Company Isothermal amplification of nucleic acids within a porous matrix
WO2016011177A1 (en) * 2014-07-18 2016-01-21 Fluidigm Corporation Reagents and kit compositions for single-cell whole genome amplification

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