WO2008157688A3 - Synthesis and use of anti-reverse phosphorothioate analogs of the messenger rna cap - Google Patents

Synthesis and use of anti-reverse phosphorothioate analogs of the messenger rna cap Download PDF

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Publication number
WO2008157688A3
WO2008157688A3 PCT/US2008/067494 US2008067494W WO2008157688A3 WO 2008157688 A3 WO2008157688 A3 WO 2008157688A3 US 2008067494 W US2008067494 W US 2008067494W WO 2008157688 A3 WO2008157688 A3 WO 2008157688A3
Authority
WO
WIPO (PCT)
Prior art keywords
analogs
arcas
cap
reverse
synthesis
Prior art date
Application number
PCT/US2008/067494
Other languages
French (fr)
Other versions
WO2008157688A2 (en
Inventor
Jacek Jemielity
Ewa M Grudzien-Nogalska
Joanna Kowalska
Edward Darzynkiewicz
Robert E Rhoads
Original Assignee
Univ Louisiana State
Jacek Jemielity
Ewa M Grudzien-Nogalska
Joanna Kowalska
Edward Darzynkiewicz
Robert E Rhoads
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to US12/280,282 priority Critical patent/US8153773B2/en
Application filed by Univ Louisiana State, Jacek Jemielity, Ewa M Grudzien-Nogalska, Joanna Kowalska, Edward Darzynkiewicz, Robert E Rhoads filed Critical Univ Louisiana State
Priority to DK08771474.7T priority patent/DK2167523T3/en
Priority to PL08771474T priority patent/PL2167523T3/en
Priority to SI200831256T priority patent/SI2167523T1/en
Priority to ES08771474.7T priority patent/ES2500515T3/en
Priority to CA2692906A priority patent/CA2692906C/en
Priority to EA201070030A priority patent/EA017740B1/en
Priority to AU2008265683A priority patent/AU2008265683B2/en
Priority to EP08771474.7A priority patent/EP2167523B1/en
Priority to CN200880102959.9A priority patent/CN101855231B/en
Publication of WO2008157688A2 publication Critical patent/WO2008157688A2/en
Publication of WO2008157688A3 publication Critical patent/WO2008157688A3/en
Priority to HRP20140771AT priority patent/HRP20140771T1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C07H19/207—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids the phosphoric or polyphosphoric acids being esterified by a further hydroxylic compound, e.g. flavine adenine dinucleotide or nicotinamide-adenine dinucleotide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Abstract

New RNA cap analogs are disclosed containing one or more phosphorothioates groups. The analogs also contain modifications at the 2'-O position of 7-methylguanosine that prevent them from being incorporated in the reverse orientation during in vitro synthesis of mRNA and that hence are 'anti-reverse cap analogs' (ARCAs). The ARCA modification ensures that the S atom is precisely positioned within the active sites of cap-binding proteins in both the translational and decapping machinery. The new S-ARCA analogs are resistant to in vivo decapping enzymes. Some S-ARCAs have a higher affinity for eIF4E than the corresponding analogs not containing a phosphorothioate group. When mRNAs containing the various S-ARCAs are introduced into cultured cells, some are translated as much as five¬ fold more efficiently than mRNAs synthesized with the conventional analog m7GpppG.
PCT/US2008/067494 2007-06-19 2008-06-19 Synthesis and use of anti-reverse phosphorothioate analogs of the messenger rna cap WO2008157688A2 (en)

Priority Applications (11)

Application Number Priority Date Filing Date Title
CA2692906A CA2692906C (en) 2007-06-19 2008-06-19 Synthesis and use of anti-reverse phosphorothioate analogs of the messenger rna cap
DK08771474.7T DK2167523T3 (en) 2007-06-19 2008-06-19 SYNTHESIS AND USE OF ANTI-REVERSE PHOSPHOROTHIOAT ANALOGS OF THE MESSENGER RNA CAPPEN
PL08771474T PL2167523T3 (en) 2007-06-19 2008-06-19 Synthesis and use of anti-reverse phosphorothioate analogs of the messenger rna cap
SI200831256T SI2167523T1 (en) 2007-06-19 2008-06-19 Synthesis and use of anti-reverse phosphorothioate analogs of the messenger rna cap
ES08771474.7T ES2500515T3 (en) 2007-06-19 2008-06-19 Synthesis and use of anti-reverse phosphorothioate analogues of the messenger RNA cap
US12/280,282 US8153773B2 (en) 2007-06-19 2008-06-19 Synthesis and use of anti-reverse phosphorothioate analogs of the messenger RNA cap
EA201070030A EA017740B1 (en) 2007-06-19 2008-06-19 Synthesis and use of anti-reverse phosphorothioate analogs of the messenger rna cap
CN200880102959.9A CN101855231B (en) 2007-06-19 2008-06-19 Synthesis and use of anti-reverse phosphorothioate analogs of the messenger RNA cap
EP08771474.7A EP2167523B1 (en) 2007-06-19 2008-06-19 Synthesis and use of anti-reverse phosphorothioate analogs of the messenger rna cap
AU2008265683A AU2008265683B2 (en) 2007-06-19 2008-06-19 Synthesis and use of anti-reverse phosphorothioate analogs of the messenger RNA cap
HRP20140771AT HRP20140771T1 (en) 2007-06-19 2014-08-18 Synthesis and use of anti-reverse phosphorothioate analogs of the messenger rna cap

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US94484207P 2007-06-19 2007-06-19
US60/944,842 2007-06-19

Publications (2)

Publication Number Publication Date
WO2008157688A2 WO2008157688A2 (en) 2008-12-24
WO2008157688A3 true WO2008157688A3 (en) 2009-02-26

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2008/067494 WO2008157688A2 (en) 2007-06-19 2008-06-19 Synthesis and use of anti-reverse phosphorothioate analogs of the messenger rna cap

Country Status (15)

Country Link
US (1) US8153773B2 (en)
EP (1) EP2167523B1 (en)
CN (3) CN104072561B (en)
AU (1) AU2008265683B2 (en)
CA (1) CA2692906C (en)
CY (1) CY1115525T1 (en)
DK (1) DK2167523T3 (en)
EA (1) EA017740B1 (en)
ES (1) ES2500515T3 (en)
HK (1) HK1200461A1 (en)
HR (1) HRP20140771T1 (en)
PL (1) PL2167523T3 (en)
PT (1) PT2167523E (en)
SI (1) SI2167523T1 (en)
WO (1) WO2008157688A2 (en)

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