WO2009009127A3 - Method and system for transferring and/or concentrating a sample - Google Patents

Method and system for transferring and/or concentrating a sample Download PDF

Info

Publication number
WO2009009127A3
WO2009009127A3 PCT/US2008/008530 US2008008530W WO2009009127A3 WO 2009009127 A3 WO2009009127 A3 WO 2009009127A3 US 2008008530 W US2008008530 W US 2008008530W WO 2009009127 A3 WO2009009127 A3 WO 2009009127A3
Authority
WO
WIPO (PCT)
Prior art keywords
sample
membrane
concentrating
chamber
transferring
Prior art date
Application number
PCT/US2008/008530
Other languages
French (fr)
Other versions
WO2009009127A2 (en
Inventor
Richard Allen
Randall E Burton
Bryan Crane
Jeffrey R Krogmeier
Jonathan W Larson
Vyacheslav Papkov
Nicaulas Sabourin
Qun Zhong
Yi Zhou
Original Assignee
Us Genomics Inc
Richard Allen
Randall E Burton
Bryan Crane
Jeffrey R Krogmeier
Jonathan W Larson
Vyacheslav Papkov
Nicaulas Sabourin
Qun Zhong
Yi Zhou
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Us Genomics Inc, Richard Allen, Randall E Burton, Bryan Crane, Jeffrey R Krogmeier, Jonathan W Larson, Vyacheslav Papkov, Nicaulas Sabourin, Qun Zhong, Yi Zhou filed Critical Us Genomics Inc
Priority to US12/668,579 priority Critical patent/US20100294665A1/en
Publication of WO2009009127A2 publication Critical patent/WO2009009127A2/en
Publication of WO2009009127A3 publication Critical patent/WO2009009127A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N2001/4038—Concentrating samples electric methods, e.g. electromigration, electrophoresis, ionisation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
    • G01N2001/4088—Concentrating samples by other techniques involving separation of suspended solids filtration

Abstract

A system for transferring and/or concentrating a sample is provided. The system includes a chamber with a membrane positioned within the chamber. An electrode assembly is configured to create an electric field across the membrane to move a charged sample through the chamber such that the sample collects and may concentrate on the membrane. The system may include a plurality of membranes. The system may also include a plurality of microchannels outwardly extending from the channel, where the membrane extends along the plurality of microchannels. Aspects of the invention are also directed to a pipette which may be used to transfer and concentrate a sample on a membrane. Certain embodiments are directed to methods and systems for concentrating a nucleic acid sample.
PCT/US2008/008530 2007-07-12 2008-07-11 Method and system for transferring and/or concentrating a sample WO2009009127A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US12/668,579 US20100294665A1 (en) 2007-07-12 2008-07-11 Method and system for transferring and/or concentrating a sample

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US95930207P 2007-07-12 2007-07-12
US60/959,302 2007-07-12

Publications (2)

Publication Number Publication Date
WO2009009127A2 WO2009009127A2 (en) 2009-01-15
WO2009009127A3 true WO2009009127A3 (en) 2009-04-09

Family

ID=40229358

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2008/008530 WO2009009127A2 (en) 2007-07-12 2008-07-11 Method and system for transferring and/or concentrating a sample

Country Status (2)

Country Link
US (1) US20100294665A1 (en)
WO (1) WO2009009127A2 (en)

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SG194070A1 (en) * 2011-04-22 2013-11-29 Sony Corp Method for electrophoresing nucleic acids, method for concentrating and purifying nucleic acids, cartridge for nucleic acid electrophoresis, and method for producing cartridge for nucleic acid electrophoresis
US9028776B2 (en) 2012-04-18 2015-05-12 Toxic Report Llc Device for stretching a polymer in a fluid sample
US8685708B2 (en) 2012-04-18 2014-04-01 Pathogenetix, Inc. Device for preparing a sample
US8956815B2 (en) 2012-04-18 2015-02-17 Toxic Report Llc Intercalation methods and devices
CN106211762B (en) 2014-04-04 2020-08-14 麻省理工学院 Active transport of charged molecules into, in and/or from a charged matrix
CN112226360A (en) * 2020-08-14 2021-01-15 南京原码科技合伙企业(有限合伙) System and method for automatically detecting pathogens in breath
CN111912697B (en) * 2020-08-14 2023-03-07 南京原码科技合伙企业(有限合伙) Rapid concentration device and method for pathogenic microorganisms

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Also Published As

Publication number Publication date
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WO2009009127A2 (en) 2009-01-15

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