WO2009009139A3 - Rna ligase polypeptides and methods of selection and use thereof - Google Patents

Rna ligase polypeptides and methods of selection and use thereof Download PDF

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Publication number
WO2009009139A3
WO2009009139A3 PCT/US2008/008550 US2008008550W WO2009009139A3 WO 2009009139 A3 WO2009009139 A3 WO 2009009139A3 US 2008008550 W US2008008550 W US 2008008550W WO 2009009139 A3 WO2009009139 A3 WO 2009009139A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
selection
rna ligase
polypeptides
ligase polypeptides
Prior art date
Application number
PCT/US2008/008550
Other languages
French (fr)
Other versions
WO2009009139A2 (en
Inventor
Burchard Seelig
Jack W Szostak
Anthony D Keefe
Glen S Cho
Original Assignee
Gen Hospital Corp
Burchard Seelig
Jack W Szostak
Anthony D Keefe
Glen S Cho
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gen Hospital Corp, Burchard Seelig, Jack W Szostak, Anthony D Keefe, Glen S Cho filed Critical Gen Hospital Corp
Publication of WO2009009139A2 publication Critical patent/WO2009009139A2/en
Publication of WO2009009139A3 publication Critical patent/WO2009009139A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)

Abstract

The present invention features polypeptides capable of catalyzing a ligation reaction between a first RNA molecule comprising, e.g., a hydroxyl group at the 3' position and a second RNA molecule comprising, e.g., a triphosphate group at the 5' position. The invention further features methods of selecting, optimizing, and using such polypeptides.
PCT/US2008/008550 2007-07-11 2008-07-11 Rna ligase polypeptides and methods of selection and use thereof WO2009009139A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US95908407P 2007-07-11 2007-07-11
US60/959,084 2007-07-11

Publications (2)

Publication Number Publication Date
WO2009009139A2 WO2009009139A2 (en) 2009-01-15
WO2009009139A3 true WO2009009139A3 (en) 2009-05-28

Family

ID=40229362

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2008/008550 WO2009009139A2 (en) 2007-07-11 2008-07-11 Rna ligase polypeptides and methods of selection and use thereof

Country Status (1)

Country Link
WO (1) WO2009009139A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013028902A2 (en) * 2011-08-23 2013-02-28 University Of Medicine And Dentistry Of New Jersey Methods of isolating rna and mapping of polyadenylation isoforms

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5763594A (en) * 1994-09-02 1998-06-09 Andrew C. Hiatt 3' protected nucleotides for enzyme catalyzed template-independent creation of phosphodiester bonds
US6696473B2 (en) * 2001-12-21 2004-02-24 X-Ceptor Therapeutics, Inc. Heterocyclic modulators of nuclear receptors
US20050113292A1 (en) * 2003-07-18 2005-05-26 Vanderbilt University Compositions of protein mimetics and methods of using same against HIV-1, SARS-coV and the like
US20050266439A1 (en) * 2002-09-20 2005-12-01 Sigridur Hjorleifsdottir Thermostable RNA ligase from thermus phage
US20060281113A1 (en) * 2005-05-18 2006-12-14 George Church Accessible polynucleotide libraries and methods of use thereof

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5763594A (en) * 1994-09-02 1998-06-09 Andrew C. Hiatt 3' protected nucleotides for enzyme catalyzed template-independent creation of phosphodiester bonds
US6696473B2 (en) * 2001-12-21 2004-02-24 X-Ceptor Therapeutics, Inc. Heterocyclic modulators of nuclear receptors
US20050266439A1 (en) * 2002-09-20 2005-12-01 Sigridur Hjorleifsdottir Thermostable RNA ligase from thermus phage
US20050113292A1 (en) * 2003-07-18 2005-05-26 Vanderbilt University Compositions of protein mimetics and methods of using same against HIV-1, SARS-coV and the like
US20060281113A1 (en) * 2005-05-18 2006-12-14 George Church Accessible polynucleotide libraries and methods of use thereof

Also Published As

Publication number Publication date
WO2009009139A2 (en) 2009-01-15

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