WO2009034186A3 - Process for cell cultivation - Google Patents
Process for cell cultivation Download PDFInfo
- Publication number
- WO2009034186A3 WO2009034186A3 PCT/EP2008/062212 EP2008062212W WO2009034186A3 WO 2009034186 A3 WO2009034186 A3 WO 2009034186A3 EP 2008062212 W EP2008062212 W EP 2008062212W WO 2009034186 A3 WO2009034186 A3 WO 2009034186A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- bio
- reactor
- cell culture
- inner surfaces
- provides
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/14—Scaffolds; Matrices
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/10—Rotating vessel
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/16—Vibrating; Shaking; Tilting
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
- C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/10—Mineral substrates
- C12N2533/18—Calcium salts, e.g. apatite, Mineral components from bones, teeth, shells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/30—Synthetic polymers
- C12N2533/40—Polyhydroxyacids, e.g. polymers of glycolic or lactic acid (PGA, PLA, PLGA); Bioresorbable polymers
Abstract
The present invention provides a process for the production of a structurally stable porous matrix material, the inner surfaces of which are at least partially colonized by adhering cells, the process involving the cultivation of mammalian cells under cell culture conditions in cell culture medium in the bio-reactor, which is agitated by a rocking movement. Accordingly, the bio-reactor used in the process of the invention is not provided with movable stirrers or static mixing elements, but rather provides essentially flat or curved inner surfaces, i.e. without elements projecting or protruding over the inner surface of the bio-reactor.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP07116301 | 2007-09-13 | ||
EP07116301.8 | 2007-09-13 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2009034186A2 WO2009034186A2 (en) | 2009-03-19 |
WO2009034186A3 true WO2009034186A3 (en) | 2010-03-11 |
Family
ID=40452607
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/EP2008/062212 WO2009034186A2 (en) | 2007-09-13 | 2008-09-12 | Process for cell cultivation |
Country Status (1)
Country | Link |
---|---|
WO (1) | WO2009034186A2 (en) |
Families Citing this family (21)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8399245B2 (en) | 2009-02-18 | 2013-03-19 | Terumo Bct, Inc. | Rotation system for cell growth chamber of a cell expansion system and method of use therefor |
WO2011090605A2 (en) * | 2009-12-29 | 2011-07-28 | Caridianbct, Inc. | Method of loading and distributing cells in a bioreactor of a cell expansion system |
US9534206B2 (en) | 2010-12-16 | 2017-01-03 | General Electric Company | Cell carrier, associated methods for making cell carrier and culturing cells using the same |
US9926523B2 (en) | 2010-12-16 | 2018-03-27 | General Electric Company | Cell carriers and methods for culturing cells |
US9518249B2 (en) | 2010-12-16 | 2016-12-13 | General Electric Company | Cell carrier, associated methods for making cell carrier and culturing cells using the same |
US9453196B2 (en) | 2010-12-16 | 2016-09-27 | General Electric Company | Cell carrier, methods of making and use |
US9453197B2 (en) | 2010-12-16 | 2016-09-27 | General Electric Company | Methods of making cell carrier |
US20120323325A1 (en) * | 2011-06-16 | 2012-12-20 | Fulton Judith A | Autologous in situ tissue engineering |
SG10201901708SA (en) * | 2012-10-23 | 2019-03-28 | Genzyme Corp | Perfusion culturing methods and uses thereof |
WO2014130864A2 (en) | 2013-02-22 | 2014-08-28 | Genzyme Corporation | Microcarrier perfusion culturing methods and uses thereof |
SG10201806083RA (en) | 2013-02-22 | 2018-08-30 | Genzyme Corp | Microcarrier perfusion culturing methods and uses thereof |
WO2015073913A1 (en) | 2013-11-16 | 2015-05-21 | Terumo Bct, Inc. | Expanding cells in a bioreactor |
WO2015148704A1 (en) | 2014-03-25 | 2015-10-01 | Terumo Bct, Inc. | Passive replacement of media |
TWI743024B (en) | 2014-06-06 | 2021-10-21 | 美商健臻公司 | Perfusion culturing methods and uses thereof |
TWI776235B (en) | 2014-06-09 | 2022-09-01 | 美商健臻公司 | Seed train processes and uses thereof |
CA3225136A1 (en) | 2014-12-22 | 2016-06-30 | Genzyme Corporation | Methods of culturing a mammalian cell |
WO2017004592A1 (en) | 2015-07-02 | 2017-01-05 | Terumo Bct, Inc. | Cell growth with mechanical stimuli |
US11685883B2 (en) | 2016-06-07 | 2023-06-27 | Terumo Bct, Inc. | Methods and systems for coating a cell growth surface |
US11104874B2 (en) | 2016-06-07 | 2021-08-31 | Terumo Bct, Inc. | Coating a bioreactor |
CN112877210B (en) * | 2021-02-25 | 2022-07-26 | 上海揽微赛尔生物科技有限公司 | Stem cell culture perfusion device with controllable pH value |
WO2023152488A1 (en) * | 2022-02-08 | 2023-08-17 | Ivy Farm Technologies Limited | Bioreactor |
Citations (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH037575A (en) * | 1989-03-30 | 1991-01-14 | Shimadzu Corp | Apparatus for cell culture |
US5026650A (en) * | 1988-06-30 | 1991-06-25 | The United States Of Amercia As Represented By The Administrator Of The National Aeronautics And Space Administration | Horizontally rotated cell culture system with a coaxial tubular oxygenator |
DE4229325A1 (en) * | 1992-09-02 | 1994-03-03 | Heraeus Sepatech | Culture vessel for cell cultures |
US5380662A (en) * | 1990-06-01 | 1995-01-10 | Robbins Scientific Corporation | Hybridization incubator with rotisserie mechanism |
US5492826A (en) * | 1993-12-10 | 1996-02-20 | William Beaumont Hospital | Apparatus and method for seeding endothelial cells |
US5686301A (en) * | 1993-09-02 | 1997-11-11 | Heraeus Instruments Gmbh | Culture vessel for cell cultures |
DE19915610A1 (en) * | 1999-04-07 | 2000-10-19 | Augustinus Bader | Process for colonization of substrates with biological cells and colonization devices that can be used for this |
EP1260580A1 (en) * | 2001-05-21 | 2002-11-27 | Core Biotech Co., Ltd. | Cell culture tube and multiple roller tube cell culture system using the same |
US20030036192A1 (en) * | 2001-02-15 | 2003-02-20 | Vijay Singh | Disposable perfusion bioreactor for cell culture |
WO2007045619A1 (en) * | 2005-10-17 | 2007-04-26 | Hannover Med Hochschule | Tissue implant and process for its production |
-
2008
- 2008-09-12 WO PCT/EP2008/062212 patent/WO2009034186A2/en active Application Filing
Patent Citations (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5026650A (en) * | 1988-06-30 | 1991-06-25 | The United States Of Amercia As Represented By The Administrator Of The National Aeronautics And Space Administration | Horizontally rotated cell culture system with a coaxial tubular oxygenator |
JPH037575A (en) * | 1989-03-30 | 1991-01-14 | Shimadzu Corp | Apparatus for cell culture |
US5380662A (en) * | 1990-06-01 | 1995-01-10 | Robbins Scientific Corporation | Hybridization incubator with rotisserie mechanism |
DE4229325A1 (en) * | 1992-09-02 | 1994-03-03 | Heraeus Sepatech | Culture vessel for cell cultures |
US5686301A (en) * | 1993-09-02 | 1997-11-11 | Heraeus Instruments Gmbh | Culture vessel for cell cultures |
US5492826A (en) * | 1993-12-10 | 1996-02-20 | William Beaumont Hospital | Apparatus and method for seeding endothelial cells |
DE19915610A1 (en) * | 1999-04-07 | 2000-10-19 | Augustinus Bader | Process for colonization of substrates with biological cells and colonization devices that can be used for this |
US20030036192A1 (en) * | 2001-02-15 | 2003-02-20 | Vijay Singh | Disposable perfusion bioreactor for cell culture |
EP1260580A1 (en) * | 2001-05-21 | 2002-11-27 | Core Biotech Co., Ltd. | Cell culture tube and multiple roller tube cell culture system using the same |
WO2007045619A1 (en) * | 2005-10-17 | 2007-04-26 | Hannover Med Hochschule | Tissue implant and process for its production |
Non-Patent Citations (1)
Title |
---|
C. SCHWARZ, V. KLEIN ; CHAMBERS HARRAP: "Concise Dictionary", 1999, CHAMBERS HARRAP, EDINGBURGH, XP002562803, 48971 * |
Also Published As
Publication number | Publication date |
---|---|
WO2009034186A2 (en) | 2009-03-19 |
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