WO2009049007A3 - Compositions, methods and systems for rapid identification of pathogenic nucleic acids - Google Patents

Compositions, methods and systems for rapid identification of pathogenic nucleic acids Download PDF

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Publication number
WO2009049007A3
WO2009049007A3 PCT/US2008/079285 US2008079285W WO2009049007A3 WO 2009049007 A3 WO2009049007 A3 WO 2009049007A3 US 2008079285 W US2008079285 W US 2008079285W WO 2009049007 A3 WO2009049007 A3 WO 2009049007A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
compositions
systems
nucleic acids
rapid identification
Prior art date
Application number
PCT/US2008/079285
Other languages
French (fr)
Other versions
WO2009049007A2 (en
Inventor
Sharat Singh
Original Assignee
Magellan Biosciences Inc
Sharat Singh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Magellan Biosciences Inc, Sharat Singh filed Critical Magellan Biosciences Inc
Publication of WO2009049007A2 publication Critical patent/WO2009049007A2/en
Publication of WO2009049007A3 publication Critical patent/WO2009049007A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Abstract

Assays, methods, kits and the like are disclosed to detect the presence and type of pathogen present in a sample. In certain examples, the assays may use a universal probe effective to hybridize to a conserved region of a nucleic acid template in the sample, and a species specific probe effective to hybridize to a non-conserved region of a nucleic acid template in the sample. Methods using the assay and kits using the probes are also described.
PCT/US2008/079285 2007-10-10 2008-10-09 Compositions, methods and systems for rapid identification of pathogenic nucleic acids WO2009049007A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US97903007P 2007-10-10 2007-10-10
US60/979,030 2007-10-10

Publications (2)

Publication Number Publication Date
WO2009049007A2 WO2009049007A2 (en) 2009-04-16
WO2009049007A3 true WO2009049007A3 (en) 2009-05-28

Family

ID=40549829

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2008/079285 WO2009049007A2 (en) 2007-10-10 2008-10-09 Compositions, methods and systems for rapid identification of pathogenic nucleic acids

Country Status (1)

Country Link
WO (1) WO2009049007A2 (en)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2348042A1 (en) 2001-06-04 2002-12-04 Ann Huletsky Sequences for detection and identification of methicillin-resistant staphylococcus aureus
US11834720B2 (en) 2005-10-11 2023-12-05 Geneohm Sciences, Inc. Sequences for detection and identification of methicillin-resistant Staphylococcus aureus (MRSA) of MREJ types xi to xx
EP2272980A1 (en) * 2009-07-09 2011-01-12 Bioesplora SRL Molecular probes for target nucleic acid detection and quantification
BR112012009023A2 (en) 2009-10-22 2019-09-24 Biotools Biotechnologial & Medical Laboratories S A method for detection. differentiation and identification of taxon-specific eubacteria in a biological sample by sequencing and kit analysis techniques
WO2013096733A1 (en) * 2011-12-21 2013-06-27 Wake Forest University Health Sciences Compositions and methods for detecting and identifying bacteria
BR112014025384B1 (en) * 2012-04-13 2022-01-25 Becton, Dickinson And Company System and methods for performing automated assay and reflective testing on samples from the respective specimens
US20150152483A1 (en) * 2012-04-19 2015-06-04 Universidad De Chile Fast and reliable wine lactic bacteria identification method
WO2015053293A1 (en) * 2013-10-07 2015-04-16 三井化学株式会社 Pcr primer set for bacterial dna amplification, kit for detecting and/or identifying bacterial species, and method for detecting and/or identifying bacterial species
WO2016193846A2 (en) * 2015-05-29 2016-12-08 Koninklijke Philips N.V. Degenerate primer sets
RU2670278C1 (en) * 2017-12-26 2018-10-22 ООО "НекстБио" Set of synthetic ologonucleotides for quantitative determination of dna of enterobacteria, staphylococci and streptococci in mucoius membranes of vagina
CN110964840B (en) * 2018-09-30 2023-07-25 深圳华大因源医药科技有限公司 Primer group, kit and library construction method for detecting 5 blood stream infection pathogens
CN112980981B (en) * 2021-04-26 2022-08-30 北京大学第一医院 Primer and probe for skin infectious granulomatous pathogen, implementation method and detection system
CN114540544A (en) * 2021-12-24 2022-05-27 廊坊诺道中科医学检验实验室有限公司 Primer-probe combination for detecting respiratory viruses, kit and application thereof

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20010051340A1 (en) * 1999-04-30 2001-12-13 Sharat Singh Kits employing generalized target-binding e-tag probes
US20020045178A1 (en) * 2000-06-13 2002-04-18 The Trustees Of Boston University Use of nucleotide analogs in the analysis of oligonucleotide mixtures and in highly multiplexed nucleic acid sequencing
US6403319B1 (en) * 1999-08-13 2002-06-11 Yale University Analysis of sequence tags with hairpin primers
US20040005555A1 (en) * 2000-08-31 2004-01-08 Rothman Richard E. Molecular diagnosis of bactermia
US20040191823A1 (en) * 2001-10-16 2004-09-30 Carmen Virgos Universal e-tag primer and probe compositions and methods
US20070105129A1 (en) * 1994-09-12 2007-05-10 Bergeron Michel G Specific and universal probes and amplification primers to rapidly detect and identify common bacterial pathogens and antibiotic resistance genes from clinical specimens for routine diagnosis in microbiology laboratories

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070105129A1 (en) * 1994-09-12 2007-05-10 Bergeron Michel G Specific and universal probes and amplification primers to rapidly detect and identify common bacterial pathogens and antibiotic resistance genes from clinical specimens for routine diagnosis in microbiology laboratories
US20010051340A1 (en) * 1999-04-30 2001-12-13 Sharat Singh Kits employing generalized target-binding e-tag probes
US6403319B1 (en) * 1999-08-13 2002-06-11 Yale University Analysis of sequence tags with hairpin primers
US20020045178A1 (en) * 2000-06-13 2002-04-18 The Trustees Of Boston University Use of nucleotide analogs in the analysis of oligonucleotide mixtures and in highly multiplexed nucleic acid sequencing
US20040005555A1 (en) * 2000-08-31 2004-01-08 Rothman Richard E. Molecular diagnosis of bactermia
US20040191823A1 (en) * 2001-10-16 2004-09-30 Carmen Virgos Universal e-tag primer and probe compositions and methods

Also Published As

Publication number Publication date
WO2009049007A2 (en) 2009-04-16

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