WO2009053875A3 - Barrier layer over electrodes of electrically active loc devices - Google Patents

Barrier layer over electrodes of electrically active loc devices Download PDF

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Publication number
WO2009053875A3
WO2009053875A3 PCT/IB2008/054272 IB2008054272W WO2009053875A3 WO 2009053875 A3 WO2009053875 A3 WO 2009053875A3 IB 2008054272 W IB2008054272 W IB 2008054272W WO 2009053875 A3 WO2009053875 A3 WO 2009053875A3
Authority
WO
WIPO (PCT)
Prior art keywords
layer
cells
electric field
barrier layer
layer over
Prior art date
Application number
PCT/IB2008/054272
Other languages
French (fr)
Other versions
WO2009053875A2 (en
Inventor
Stefano Cattaneo
Murray Fulton Gillies
Hoog Thomas Jan De
Ralph Kurt
Roel Penterman
Original Assignee
Koninkl Philips Electronics Nv
Stefano Cattaneo
Murray Fulton Gillies
Hoog Thomas Jan De
Ralph Kurt
Roel Penterman
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Koninkl Philips Electronics Nv, Stefano Cattaneo, Murray Fulton Gillies, Hoog Thomas Jan De, Ralph Kurt, Roel Penterman filed Critical Koninkl Philips Electronics Nv
Publication of WO2009053875A2 publication Critical patent/WO2009053875A2/en
Publication of WO2009053875A3 publication Critical patent/WO2009053875A3/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C5/00—Separating dispersed particles from liquids by electrostatic effect
    • B03C5/02—Separators
    • B03C5/022—Non-uniform field separators
    • B03C5/026—Non-uniform field separators using open-gradient differential dielectric separation, i.e. using electrodes of special shapes for non-uniform field creation, e.g. Fluid Integrated Circuit [FIC]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5029—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on cell motility

Abstract

A barrier layer, e.g. an electrically permeable layer such as a hydrogel layer (30) is provided on electrodes (50) of a dielectrophoretic device for the purpose of preserving the viability of cells and in particular preventing electric field-induced cell damage and lysis. The barrier layer provides a physical barrier that keeps the cells at a certain distance from the surface of the electrodes, where the electric field has the highest intensity. In contrast to an insulating layer, the hydrogel layer is essentially permeable to the electric field. The electric field on the surface of the layer is therefore still sufficient for the dielectrophoretic manipulation of the cells such as trapping the cells in a field cage. The device can be integrated into Lab-on-chip (LOC) microsystems.
PCT/IB2008/054272 2007-10-22 2008-10-17 Barrier layer over electrodes of electrically active loc devices WO2009053875A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
EP07118964A EP2053396A1 (en) 2007-10-22 2007-10-22 Device and method for the monitoring of the movement of cells
EP07118964.1 2007-10-22
EP07119255A EP2052784A1 (en) 2007-10-22 2007-10-25 Barrier layer over electrodes of electrically active loc devices
EP07119255.3 2007-10-25

Publications (2)

Publication Number Publication Date
WO2009053875A2 WO2009053875A2 (en) 2009-04-30
WO2009053875A3 true WO2009053875A3 (en) 2009-08-13

Family

ID=39410488

Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/IB2008/054272 WO2009053875A2 (en) 2007-10-22 2008-10-17 Barrier layer over electrodes of electrically active loc devices
PCT/IB2008/054293 WO2009053880A1 (en) 2007-10-22 2008-10-20 Device and method for the monitoring of the movement of cells

Family Applications After (1)

Application Number Title Priority Date Filing Date
PCT/IB2008/054293 WO2009053880A1 (en) 2007-10-22 2008-10-20 Device and method for the monitoring of the movement of cells

Country Status (2)

Country Link
EP (2) EP2053396A1 (en)
WO (2) WO2009053875A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9889445B2 (en) 2013-10-22 2018-02-13 Berkeley Lights, Inc. Micro-fluidic devices for assaying biological activity
CN109142717B (en) * 2013-10-22 2021-11-16 伯克利之光生命科技公司 Microfluidic device for determining biological activity
TWI522082B (en) * 2013-12-24 2016-02-21 明達醫學科技股份有限公司 Cell mobility characteristics sensing apparatus and operating method thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020155586A1 (en) * 1994-07-07 2002-10-24 Nanogen, Inc. Integrated portable biological detection system
US20030146100A1 (en) * 2002-02-06 2003-08-07 Nanogen, Inc. Dielectrophoretic separation and immunoassay methods on active electronic matrix devices
WO2007107947A1 (en) * 2006-03-21 2007-09-27 Koninklijke Philips Electronics N.V. Microelectronic device with heating electrodes

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6942776B2 (en) * 1999-05-18 2005-09-13 Silicon Biosystems S.R.L. Method and apparatus for the manipulation of particles by means of dielectrophoresis
US7033819B2 (en) * 2000-11-08 2006-04-25 Surface Logix, Inc. System for monitoring cell motility in real-time
CA2472029C (en) * 2001-11-26 2014-04-15 Keck Graduate Institute Method, apparatus and article for microfluidic control via electrowetting, for chemical, biochemical and biological assays and the like
US6972184B2 (en) * 2003-12-23 2005-12-06 Millipore Corporation Cell motility assay
DE102006002462A1 (en) * 2006-01-18 2007-07-19 Evotec Technologies Gmbh Electric field cage and associated operating method

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020155586A1 (en) * 1994-07-07 2002-10-24 Nanogen, Inc. Integrated portable biological detection system
US20030146100A1 (en) * 2002-02-06 2003-08-07 Nanogen, Inc. Dielectrophoretic separation and immunoassay methods on active electronic matrix devices
WO2007107947A1 (en) * 2006-03-21 2007-09-27 Koninklijke Philips Electronics N.V. Microelectronic device with heating electrodes

Also Published As

Publication number Publication date
WO2009053875A2 (en) 2009-04-30
WO2009053880A1 (en) 2009-04-30
EP2053396A1 (en) 2009-04-29
EP2052784A1 (en) 2009-04-29

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