WO2009133462A3 - Proteins - Google Patents

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Publication number
WO2009133462A3
WO2009133462A3 PCT/IB2009/005629 IB2009005629W WO2009133462A3 WO 2009133462 A3 WO2009133462 A3 WO 2009133462A3 IB 2009005629 W IB2009005629 W IB 2009005629W WO 2009133462 A3 WO2009133462 A3 WO 2009133462A3
Authority
WO
WIPO (PCT)
Prior art keywords
protein
interest
amino acid
signal sequence
secretion
Prior art date
Application number
PCT/IB2009/005629
Other languages
French (fr)
Other versions
WO2009133462A9 (en
WO2009133462A2 (en
Inventor
Patrick Maria Franciscus Derkx
Harm Mulder
Igor Nikolaev
Original Assignee
Danisco A/S
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB0807881A external-priority patent/GB0807881D0/en
Priority claimed from GB0807882A external-priority patent/GB0807882D0/en
Priority claimed from GB0811662A external-priority patent/GB0811662D0/en
Priority claimed from GB0817077A external-priority patent/GB0817077D0/en
Application filed by Danisco A/S filed Critical Danisco A/S
Priority to EP09738475.4A priority Critical patent/EP2279248B1/en
Publication of WO2009133462A2 publication Critical patent/WO2009133462A2/en
Publication of WO2009133462A3 publication Critical patent/WO2009133462A3/en
Priority to US12/915,204 priority patent/US8791232B2/en
Publication of WO2009133462A9 publication Critical patent/WO2009133462A9/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals

Abstract

An amino acid sequence is described. The amino acid comprises a signal sequence that is SEQ ID No. 1a (or a variant or homologue or derivative or fragment thereof) that is expressed as a fusion protein that comprisies a protein of interest. The signal sequence directs secretion of the protein of interest. The protein of interest may be a heterologous protein. Secretion of the fusion protein aids purification.
PCT/IB2009/005629 2008-04-30 2009-04-30 Proteins WO2009133462A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP09738475.4A EP2279248B1 (en) 2008-04-30 2009-04-30 Proteins
US12/915,204 US8791232B2 (en) 2008-04-30 2010-10-29 Proteins

Applications Claiming Priority (14)

Application Number Priority Date Filing Date Title
GB0807881A GB0807881D0 (en) 2008-04-30 2008-04-30 Oxidation process
GB0807882.6 2008-04-30
GB0807881.8 2008-04-30
GB0807882A GB0807882D0 (en) 2008-04-30 2008-04-30 Composition
GB0811662.6 2008-06-25
GB0811662A GB0811662D0 (en) 2008-06-25 2008-06-25 Composition
GB0817077A GB0817077D0 (en) 2008-09-17 2008-09-17 Proteins
GB0817077.1 2008-09-17
US9969808P 2008-09-24 2008-09-24
US9966708P 2008-09-24 2008-09-24
US9971508P 2008-09-24 2008-09-24
US61/099,715 2008-09-24
US61/099,667 2008-09-24
US61/099,698 2008-09-24

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US12/915,204 Continuation-In-Part US8791232B2 (en) 2008-04-30 2010-10-29 Proteins

Publications (3)

Publication Number Publication Date
WO2009133462A2 WO2009133462A2 (en) 2009-11-05
WO2009133462A3 true WO2009133462A3 (en) 2010-06-10
WO2009133462A9 WO2009133462A9 (en) 2011-02-17

Family

ID=41254811

Family Applications (3)

Application Number Title Priority Date Filing Date
PCT/IB2009/005623 WO2009133461A1 (en) 2008-04-30 2009-04-30 Composition
PCT/IB2009/005738 WO2009133464A2 (en) 2008-04-30 2009-04-30 Oxidation process
PCT/IB2009/005629 WO2009133462A2 (en) 2008-04-30 2009-04-30 Proteins

Family Applications Before (2)

Application Number Title Priority Date Filing Date
PCT/IB2009/005623 WO2009133461A1 (en) 2008-04-30 2009-04-30 Composition
PCT/IB2009/005738 WO2009133464A2 (en) 2008-04-30 2009-04-30 Oxidation process

Country Status (6)

Country Link
US (2) US8802398B2 (en)
EP (2) EP2281034B1 (en)
CN (1) CN102083996A (en)
DK (1) DK2281034T3 (en)
HK (1) HK1153507A1 (en)
WO (3) WO2009133461A1 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5860480B2 (en) 2011-01-11 2016-02-16 キャプシュゲル・ベルジウム・エヌ・ヴィ New hard capsule containing pullulan
AU2012268427B2 (en) 2011-06-06 2016-02-04 Dermaforce Holdings, LLC Skin treatments containing pyrroloquinoline quinone (PQQ) esters and methods of preparation and use thereof
CN105026548B (en) * 2012-06-04 2019-06-18 盐水港精糖株式会社 D-Glucose diacid produces the manufacturing method of bacterium and D-Glucose diacid
EP2803372A1 (en) 2013-05-16 2014-11-19 Universiteit Twente Process for the preparation of an object supporting a lipid bilayer
MX2016011467A (en) * 2014-03-11 2016-11-16 Du Pont Oxidized poly alpha-1,3-glucan as detergent builder.
BR112016021298A2 (en) * 2014-03-21 2017-10-03 Synthetic Genomics Inc COMPOSITIONS AND METHODS FOR PRODUCING CHEMICAL PRODUCTS AND DERIVATIVES THEREOF
AU2015388744B2 (en) 2015-03-27 2021-03-18 Erber Aktiengesellschaft Use of a trichothecene-transforming alcohol dehydrogenase, method for transforming trichothecenes and trichothecene-transforming additive
CN107056778B (en) * 2016-12-22 2019-05-21 上海宣创生物科技有限公司 Pyrroloquinoline quinone beet alkali salt
WO2018189587A1 (en) 2017-04-14 2018-10-18 Capsugel Belgium Nv Process for making pullulan
CN110678170A (en) 2017-04-14 2020-01-10 比利时胶囊公司 Pullulan polysaccharide capsule
EP3814433A1 (en) * 2018-06-28 2021-05-05 Basf Se Aqueous dispersions and emulsion paints comprising ethylhexyl acrylate-butyl acrylate-styrene copolymers

Family Cites Families (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL154598B (en) 1970-11-10 1977-09-15 Organon Nv PROCEDURE FOR DETERMINING AND DETERMINING LOW MOLECULAR COMPOUNDS AND PROTEINS THAT CAN SPECIFICALLY BIND THESE COMPOUNDS AND TEST PACKAGING.
US3817837A (en) 1971-05-14 1974-06-18 Syva Corp Enzyme amplification assay
US3939350A (en) 1974-04-29 1976-02-17 Board Of Trustees Of The Leland Stanford Junior University Fluorescent immunoassay employing total reflection for activation
US3996345A (en) 1974-08-12 1976-12-07 Syva Company Fluorescence quenching with immunological pairs in immunoassays
US4275149A (en) 1978-11-24 1981-06-23 Syva Company Macromolecular environment control in specific receptor assays
US4277437A (en) 1978-04-05 1981-07-07 Syva Company Kit for carrying out chemically induced fluorescence immunoassay
US4366241A (en) 1980-08-07 1982-12-28 Syva Company Concentrating zone method in heterogeneous immunoassays
NL8300698A (en) 1983-02-24 1984-09-17 Univ Leiden METHOD FOR BUILDING FOREIGN DNA INTO THE NAME OF DIABIC LOBAL PLANTS; AGROBACTERIUM TUMEFACIENS BACTERIA AND METHOD FOR PRODUCTION THEREOF; PLANTS AND PLANT CELLS WITH CHANGED GENETIC PROPERTIES; PROCESS FOR PREPARING CHEMICAL AND / OR PHARMACEUTICAL PRODUCTS.
US4675394A (en) 1984-08-17 1987-06-23 National Starch And Chemical Corporation Polysaccharide derivatives containing aldehyde groups, their preparation from the corresponding acetals and use as paper additives
DE3546807C2 (en) 1985-03-30 1991-03-28 Marc Genf/Geneve Ch Ballivet
US4663448A (en) 1985-10-23 1987-05-05 National Starch And Chemical Corporation Aldehyde-containing heterpolysaccharides, a process for their preparation, and the use thereof
DK122686D0 (en) 1986-03-17 1986-03-17 Novo Industri As PREPARATION OF PROTEINS
KR100225087B1 (en) 1990-03-23 1999-10-15 한스 발터라벤 The expression of phytase in plants
EP0531372B2 (en) 1990-05-09 2004-04-14 Novozymes A/S A cellulase preparation comprising an endoglucanase enzyme
DE4112440C1 (en) 1991-04-16 1992-10-22 Diagen Institut Fuer Molekularbiologische Diagnostik Gmbh, 4000 Duesseldorf, De
TW293036B (en) 1992-11-27 1996-12-11 Takeda Pharm Industry Co Ltd
AU666443B2 (en) 1992-12-10 1996-02-08 Dsm N.V. Production of heterologous proteins in filamentous fungi
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5741665A (en) 1994-05-10 1998-04-21 University Of Hawaii Light-regulated promoters for production of heterologous proteins in filamentous fungi
GB2306473B (en) * 1995-10-26 1998-12-23 Nippon Paint Co Ltd Glucoxide derivatives for enzyme modification, lipid-coated enzymes, method of producing such enzymes and antifouling paint composition
DE69735199T2 (en) 1996-03-27 2006-09-21 Novozymes A/S ALKALINE PHOSPHATASE DEFICIENT, FILAMENTOUS MUSHROOMS
FI105690B (en) 1997-11-04 2000-09-29 Valtion Teknillinen Process for the preparation of oxidized starch
US6265570B1 (en) 1998-11-05 2001-07-24 National Starch & Chemical Investment Holding Corporation Cold water soluble starch aldehydes and the method of preparation thereof
DE10119274A1 (en) 2001-04-20 2002-10-31 Juelich Enzyme Products Gmbh Enzymatic process for the enantioselective reduction of keto compounds
US6710175B2 (en) 2001-08-31 2004-03-23 Kevin Ray Anderson Compositions suitable as additives in the paper industry, preparation; use; and, paper comprising such
JP2003159079A (en) * 2001-11-29 2003-06-03 Fujisawa Pharmaceut Co Ltd Alcohol/aldehyde dehydrogenase, method for producing the same and use thereof
WO2003087234A1 (en) * 2002-04-12 2003-10-23 Biolocus Aps Antifouling composition comprising an enzyme in the absence of its substrate
US20030228672A1 (en) * 2002-06-06 2003-12-11 Archer-Daniels-Midland Company Sorbitol dehydrogenases of ketogulonigenium species, genes and methods of use thereof
US20060198819A1 (en) 2003-08-08 2006-09-07 Novo Nordisk Healthcare A/G Use of galactose oxidase for selective chemical conjugation of protractor molecules to proteins of therapeutic interest

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BROCKMEIER U ET AL: "Systematic Screening of All Signal Peptides from Bacillus subtilis: A Powerful Strategy in Optimizing Heterologous Protein Secretion in Gram-positive Bacteria", JOURNAL OF MOLECULAR BIOLOGY, LONDON, GB, vol. 362, no. 3, 22 September 2006 (2006-09-22), pages 393 - 402, XP024951379, ISSN: 0022-2836, [retrieved on 20060922] *
SHIBATA T ET AL: "Purification and molecular characterization of a quinoprotein alcohol dehydrogenase from Pseudogluconobacter saccharoketogenes IFO 14464", JOURNAL OF BIOSCIENCE AND BIOENGINEERING 2001,, vol. 92, no. 6, 1 January 2001 (2001-01-01), pages 524 - 531, XP002551504 *
TAN N S ET AL: "Engineering a Novel Secretion Signal for Cross-Host Recombinant Protein Expression", PROTEIN ENGINEERING, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 15, no. 4, 1 January 2002 (2002-01-01), pages 337 - 345, XP003010339, ISSN: 0269-2139 *

Also Published As

Publication number Publication date
US20110236935A1 (en) 2011-09-29
US8802398B2 (en) 2014-08-12
WO2009133461A1 (en) 2009-11-05
WO2009133462A9 (en) 2011-02-17
US20110256571A1 (en) 2011-10-20
CN102083996A (en) 2011-06-01
WO2009133462A2 (en) 2009-11-05
EP2279248B1 (en) 2015-04-01
EP2279248A2 (en) 2011-02-02
EP2281034B1 (en) 2015-10-21
WO2009133464A2 (en) 2009-11-05
WO2009133464A9 (en) 2011-01-20
HK1153507A1 (en) 2012-03-30
EP2281034A2 (en) 2011-02-09
WO2009133464A3 (en) 2010-03-25
DK2281034T3 (en) 2016-01-25
US8791232B2 (en) 2014-07-29

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