WO2009147486A3 - Micro-fluidic device for measurements of cell migration in response to chemical stimuli - Google Patents
Micro-fluidic device for measurements of cell migration in response to chemical stimuli Download PDFInfo
- Publication number
- WO2009147486A3 WO2009147486A3 PCT/IB2009/005712 IB2009005712W WO2009147486A3 WO 2009147486 A3 WO2009147486 A3 WO 2009147486A3 IB 2009005712 W IB2009005712 W IB 2009005712W WO 2009147486 A3 WO2009147486 A3 WO 2009147486A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- site
- micro
- response
- cells
- measurements
- Prior art date
Links
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
- G01N33/5029—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on cell motility
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0829—Multi-well plates; Microtitration plates
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0887—Laminated structure
Abstract
The invention relates to a device for making measurements of the migration of cells in response to exposure to a gradient of concentration of a chemoat tractive substance (chemotaxis). In particular, the invention relates to a device comprising a first site designed to receive a specimen of cells, and at least one second site designed to receive a substance potentially capable of influencing chemotaxis, wherein the first and second sites are fluidically connected together by a containing duct, and are arranged along an axis A directed between the first site and the second site with a non-uniform density, a plurality of obstacle elements that create along the duct a gradient in the free section of passage at the disposal of the cells, which is defined by a corresponding plurality of micro- channels directed parallel to the axis A and having a pre-set transverse dimension d.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ITTO2008A000396 | 2008-05-26 | ||
ITTO20080396 ITTO20080396A1 (en) | 2008-05-26 | 2008-05-26 | MICROFLUID DEVICE FOR CELL MIGRATION MEASUREMENTS IN RESPONSE TO CHEMICAL STIMULI |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2009147486A2 WO2009147486A2 (en) | 2009-12-10 |
WO2009147486A3 true WO2009147486A3 (en) | 2010-01-28 |
Family
ID=40303125
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/IB2009/005712 WO2009147486A2 (en) | 2008-05-26 | 2009-05-25 | Micro-fluidic device for measurements of cell migration in response to chemical stimuli |
Country Status (2)
Country | Link |
---|---|
IT (1) | ITTO20080396A1 (en) |
WO (1) | WO2009147486A2 (en) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR101267274B1 (en) | 2011-08-25 | 2013-05-23 | 전남대학교산학협력단 | An apparatus and a method for analyzing chemotaxis of cells or cell-based microrobots |
WO2015009688A1 (en) | 2013-07-17 | 2015-01-22 | The Johns Hopkins University | Microfluidic chip for analysis of cell motility and methods for using same |
WO2017075297A1 (en) * | 2015-10-28 | 2017-05-04 | The Broad Institute Inc. | High-throughput dynamic reagent delivery system |
US20220126291A1 (en) * | 2020-10-23 | 2022-04-28 | Applied Materials, Inc. | High throughput compact microfluidic cell counter |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5744366A (en) * | 1992-05-01 | 1998-04-28 | Trustees Of The University Of Pennsylvania | Mesoscale devices and methods for analysis of motile cells |
US20030022362A1 (en) * | 2000-11-08 | 2003-01-30 | Gregory Kirk | Cell motility and chemotaxis test device and methods of using same |
WO2004037374A2 (en) * | 2002-10-23 | 2004-05-06 | The Trustees Of Princeton University | Method for continuous particle separation using obstacle arrays asymmetrically aligned to fields |
JP2006007007A (en) * | 2004-06-22 | 2006-01-12 | Sharp Corp | Microreactor |
US20070026469A1 (en) * | 2005-07-29 | 2007-02-01 | Martin Fuchs | Devices and methods for enrichment and alteration of circulating tumor cells and other particles |
-
2008
- 2008-05-26 IT ITTO20080396 patent/ITTO20080396A1/en unknown
-
2009
- 2009-05-25 WO PCT/IB2009/005712 patent/WO2009147486A2/en active Application Filing
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5744366A (en) * | 1992-05-01 | 1998-04-28 | Trustees Of The University Of Pennsylvania | Mesoscale devices and methods for analysis of motile cells |
US20030022362A1 (en) * | 2000-11-08 | 2003-01-30 | Gregory Kirk | Cell motility and chemotaxis test device and methods of using same |
WO2004037374A2 (en) * | 2002-10-23 | 2004-05-06 | The Trustees Of Princeton University | Method for continuous particle separation using obstacle arrays asymmetrically aligned to fields |
JP2006007007A (en) * | 2004-06-22 | 2006-01-12 | Sharp Corp | Microreactor |
US20070026469A1 (en) * | 2005-07-29 | 2007-02-01 | Martin Fuchs | Devices and methods for enrichment and alteration of circulating tumor cells and other particles |
Also Published As
Publication number | Publication date |
---|---|
ITTO20080396A1 (en) | 2009-11-27 |
WO2009147486A2 (en) | 2009-12-10 |
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