WO2009147486A3 - Micro-fluidic device for measurements of cell migration in response to chemical stimuli - Google Patents

Micro-fluidic device for measurements of cell migration in response to chemical stimuli Download PDF

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Publication number
WO2009147486A3
WO2009147486A3 PCT/IB2009/005712 IB2009005712W WO2009147486A3 WO 2009147486 A3 WO2009147486 A3 WO 2009147486A3 IB 2009005712 W IB2009005712 W IB 2009005712W WO 2009147486 A3 WO2009147486 A3 WO 2009147486A3
Authority
WO
WIPO (PCT)
Prior art keywords
site
micro
response
cells
measurements
Prior art date
Application number
PCT/IB2009/005712
Other languages
French (fr)
Other versions
WO2009147486A2 (en
Inventor
Marco Pizzi
Emilio Hirsch
Carlotta Costa
Elisa Vitiello
Original Assignee
Techfab S.R.L.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Techfab S.R.L. filed Critical Techfab S.R.L.
Publication of WO2009147486A2 publication Critical patent/WO2009147486A2/en
Publication of WO2009147486A3 publication Critical patent/WO2009147486A3/en

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5029—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on cell motility
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0887—Laminated structure

Abstract

The invention relates to a device for making measurements of the migration of cells in response to exposure to a gradient of concentration of a chemoat tractive substance (chemotaxis). In particular, the invention relates to a device comprising a first site designed to receive a specimen of cells, and at least one second site designed to receive a substance potentially capable of influencing chemotaxis, wherein the first and second sites are fluidically connected together by a containing duct, and are arranged along an axis A directed between the first site and the second site with a non-uniform density, a plurality of obstacle elements that create along the duct a gradient in the free section of passage at the disposal of the cells, which is defined by a corresponding plurality of micro- channels directed parallel to the axis A and having a pre-set transverse dimension d.
PCT/IB2009/005712 2008-05-26 2009-05-25 Micro-fluidic device for measurements of cell migration in response to chemical stimuli WO2009147486A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ITTO2008A000396 2008-05-26
ITTO20080396 ITTO20080396A1 (en) 2008-05-26 2008-05-26 MICROFLUID DEVICE FOR CELL MIGRATION MEASUREMENTS IN RESPONSE TO CHEMICAL STIMULI

Publications (2)

Publication Number Publication Date
WO2009147486A2 WO2009147486A2 (en) 2009-12-10
WO2009147486A3 true WO2009147486A3 (en) 2010-01-28

Family

ID=40303125

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2009/005712 WO2009147486A2 (en) 2008-05-26 2009-05-25 Micro-fluidic device for measurements of cell migration in response to chemical stimuli

Country Status (2)

Country Link
IT (1) ITTO20080396A1 (en)
WO (1) WO2009147486A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101267274B1 (en) 2011-08-25 2013-05-23 전남대학교산학협력단 An apparatus and a method for analyzing chemotaxis of cells or cell-based microrobots
WO2015009688A1 (en) 2013-07-17 2015-01-22 The Johns Hopkins University Microfluidic chip for analysis of cell motility and methods for using same
WO2017075297A1 (en) * 2015-10-28 2017-05-04 The Broad Institute Inc. High-throughput dynamic reagent delivery system
US20220126291A1 (en) * 2020-10-23 2022-04-28 Applied Materials, Inc. High throughput compact microfluidic cell counter

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5744366A (en) * 1992-05-01 1998-04-28 Trustees Of The University Of Pennsylvania Mesoscale devices and methods for analysis of motile cells
US20030022362A1 (en) * 2000-11-08 2003-01-30 Gregory Kirk Cell motility and chemotaxis test device and methods of using same
WO2004037374A2 (en) * 2002-10-23 2004-05-06 The Trustees Of Princeton University Method for continuous particle separation using obstacle arrays asymmetrically aligned to fields
JP2006007007A (en) * 2004-06-22 2006-01-12 Sharp Corp Microreactor
US20070026469A1 (en) * 2005-07-29 2007-02-01 Martin Fuchs Devices and methods for enrichment and alteration of circulating tumor cells and other particles

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5744366A (en) * 1992-05-01 1998-04-28 Trustees Of The University Of Pennsylvania Mesoscale devices and methods for analysis of motile cells
US20030022362A1 (en) * 2000-11-08 2003-01-30 Gregory Kirk Cell motility and chemotaxis test device and methods of using same
WO2004037374A2 (en) * 2002-10-23 2004-05-06 The Trustees Of Princeton University Method for continuous particle separation using obstacle arrays asymmetrically aligned to fields
JP2006007007A (en) * 2004-06-22 2006-01-12 Sharp Corp Microreactor
US20070026469A1 (en) * 2005-07-29 2007-02-01 Martin Fuchs Devices and methods for enrichment and alteration of circulating tumor cells and other particles

Also Published As

Publication number Publication date
ITTO20080396A1 (en) 2009-11-27
WO2009147486A2 (en) 2009-12-10

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