WO2010034017A3 - Systems and methods for signal normalization using raman scattering - Google Patents

Systems and methods for signal normalization using raman scattering Download PDF

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Publication number
WO2010034017A3
WO2010034017A3 PCT/US2009/057914 US2009057914W WO2010034017A3 WO 2010034017 A3 WO2010034017 A3 WO 2010034017A3 US 2009057914 W US2009057914 W US 2009057914W WO 2010034017 A3 WO2010034017 A3 WO 2010034017A3
Authority
WO
WIPO (PCT)
Prior art keywords
normalization
detection
capillary
systems
methods
Prior art date
Application number
PCT/US2009/057914
Other languages
French (fr)
Other versions
WO2010034017A2 (en
Inventor
Dmitry Sagatelyan
Original Assignee
Life Technologies Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Life Technologies Corporation filed Critical Life Technologies Corporation
Publication of WO2010034017A2 publication Critical patent/WO2010034017A2/en
Publication of WO2010034017A3 publication Critical patent/WO2010034017A3/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J3/44—Raman spectrometry; Scattering spectrometry ; Fluorescence spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/27—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection ; circuits for computing concentration
    • G01N21/274—Calibration, base line adjustment, drift correction
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/65—Raman scattering

Abstract

Systems and methods for normalizing signals from detection zones of a biological analysis device are disclosed. In certain embodiments, signals from a plurality of detection zones in a capillary electrophoresis device are normalized based on detection and analysis of a Raman line resulting from Raman scattering of excitation light from the buffer solution in the detection zones. Such normalization can account for systematic variations that, if not corrected, do not allow quantitative analysis of results from different capillaries. In certain embodiments, such normalization can be achieved using the same excitation light and detection zone as used for detecting analyte samples such as dye-labeled DNA fragments. Further, such normalization can be incorporated into an existing calibration file, such that capillary-to-capillary systematic variations can be corrected with minimum or no modifications to existing hardware and/or data collection software.
PCT/US2009/057914 2008-09-22 2009-09-22 Systems and methods for signal normalization using raman scattering WO2010034017A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US9892508P 2008-09-22 2008-09-22
US61/098,925 2008-09-22

Publications (2)

Publication Number Publication Date
WO2010034017A2 WO2010034017A2 (en) 2010-03-25
WO2010034017A3 true WO2010034017A3 (en) 2010-06-17

Family

ID=42040207

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2009/057914 WO2010034017A2 (en) 2008-09-22 2009-09-22 Systems and methods for signal normalization using raman scattering

Country Status (1)

Country Link
WO (1) WO2010034017A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102411024B (en) * 2011-06-15 2014-07-09 公安部第一研究所 Capillary array electrophoresis detection method based on spatial correction and spectral correction
CN102331415B (en) * 2011-06-15 2014-06-04 公安部第一研究所 Method for positioning capillary tube array by using raman spectral imaging
CN103308491A (en) * 2013-05-29 2013-09-18 浙江大学 Multi-camera synchronously-tracked photoluminescence solar battery detecting device

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5455673A (en) * 1994-05-27 1995-10-03 Eastman Chemical Company Apparatus and method for measuring and applying a convolution function to produce a standard Raman spectrum
US6774992B1 (en) * 1997-03-10 2004-08-10 Alberta Research Council Inc. Determination of the property of a solution or solid using raman ratios
US20060055919A1 (en) * 2004-09-15 2006-03-16 Yuan-Hsiang Lee Apparatus and method for producing a calibrated raman spectrum
US20070071649A1 (en) * 2001-09-10 2007-03-29 Marcus R Kenneth Capillary-channeled polymer fibers as stationary phase media for spectroscopic analysis

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5455673A (en) * 1994-05-27 1995-10-03 Eastman Chemical Company Apparatus and method for measuring and applying a convolution function to produce a standard Raman spectrum
US6774992B1 (en) * 1997-03-10 2004-08-10 Alberta Research Council Inc. Determination of the property of a solution or solid using raman ratios
US20070071649A1 (en) * 2001-09-10 2007-03-29 Marcus R Kenneth Capillary-channeled polymer fibers as stationary phase media for spectroscopic analysis
US20060055919A1 (en) * 2004-09-15 2006-03-16 Yuan-Hsiang Lee Apparatus and method for producing a calibrated raman spectrum

Also Published As

Publication number Publication date
WO2010034017A2 (en) 2010-03-25

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