WO2010122531A3 - Method of producing a lipolytic enzyme - Google Patents

Method of producing a lipolytic enzyme Download PDF

Info

Publication number
WO2010122531A3
WO2010122531A3 PCT/IB2010/051802 IB2010051802W WO2010122531A3 WO 2010122531 A3 WO2010122531 A3 WO 2010122531A3 IB 2010051802 W IB2010051802 W IB 2010051802W WO 2010122531 A3 WO2010122531 A3 WO 2010122531A3
Authority
WO
WIPO (PCT)
Prior art keywords
lipolytic enzyme
producing
relates
present
trichoderma
Prior art date
Application number
PCT/IB2010/051802
Other languages
French (fr)
Other versions
WO2010122531A2 (en
Inventor
Susan M Madrid
Cherry Lin
Masoud Rajabi Zargahi
Rikke Høegh Lorentsen
Mai Faurschou Isaksen
Michael Ward
Original Assignee
Danisco A/S
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=40862772&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=WO2010122531(A3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority to ES10723329T priority Critical patent/ES2616057T5/en
Priority to US13/265,712 priority patent/US8940519B2/en
Priority to CA2759405A priority patent/CA2759405C/en
Priority to NZ595833A priority patent/NZ595833A/en
Priority to EP10723329.8A priority patent/EP2421963B2/en
Application filed by Danisco A/S filed Critical Danisco A/S
Priority to AU2010240473A priority patent/AU2010240473B2/en
Priority to BRPI1006628A priority patent/BRPI1006628B8/en
Priority to DK10723329.8T priority patent/DK2421963T4/en
Priority to CN201080018179.3A priority patent/CN102439143B/en
Publication of WO2010122531A2 publication Critical patent/WO2010122531A2/en
Publication of WO2010122531A3 publication Critical patent/WO2010122531A3/en
Priority to US14/536,016 priority patent/US9382523B2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/06—Enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/189—Enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/14—Yeasts or derivatives thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01003—Triacylglycerol lipase (3.1.1.3)

Abstract

The present invention relates to a method for the production of a lipolytic enzyme in Trichoderma reesei and the lipolytic enzyme obtainable therefrom. In addition, the present invention relates to the use of Trichoderma to express a lipolytic enzyme.
PCT/IB2010/051802 2009-04-24 2010-04-23 Method WO2010122531A2 (en)

Priority Applications (10)

Application Number Priority Date Filing Date Title
CN201080018179.3A CN102439143B (en) 2009-04-24 2010-04-23 For the production of the method for lipolytic enzyme
US13/265,712 US8940519B2 (en) 2009-04-24 2010-04-23 Method of producing a lipolytic enzyme
CA2759405A CA2759405C (en) 2009-04-24 2010-04-23 Method
NZ595833A NZ595833A (en) 2009-04-24 2010-04-23 Method of producing a lipolytic enzyme
EP10723329.8A EP2421963B2 (en) 2009-04-24 2010-04-23 Method of producing a lipolytic enzyme
ES10723329T ES2616057T5 (en) 2009-04-24 2010-04-23 Production method of a lipolytic enzyme
AU2010240473A AU2010240473B2 (en) 2009-04-24 2010-04-23 Method of producing a lipolytic enzyme
BRPI1006628A BRPI1006628B8 (en) 2009-04-24 2010-04-23 method to produce a lipolytic enzyme
DK10723329.8T DK2421963T4 (en) 2009-04-24 2010-04-23 PROCEDURE FOR THE PRODUCTION OF A LIPOLYTIC ENZYME
US14/536,016 US9382523B2 (en) 2009-04-24 2014-11-07 Method of producing a lipolytic enzyme

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US17227209P 2009-04-24 2009-04-24
US61/172,272 2009-04-24
GBGB0908770.1A GB0908770D0 (en) 2009-04-24 2009-05-20 Method
GB0908770.1 2009-05-20

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US13/265,712 A-371-Of-International US8940519B2 (en) 2009-04-24 2010-04-23 Method of producing a lipolytic enzyme
US14/536,016 Continuation US9382523B2 (en) 2009-04-24 2014-11-07 Method of producing a lipolytic enzyme

Publications (2)

Publication Number Publication Date
WO2010122531A2 WO2010122531A2 (en) 2010-10-28
WO2010122531A3 true WO2010122531A3 (en) 2010-12-29

Family

ID=40862772

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2010/051802 WO2010122531A2 (en) 2009-04-24 2010-04-23 Method

Country Status (11)

Country Link
US (2) US8940519B2 (en)
EP (1) EP2421963B2 (en)
CN (1) CN102439143B (en)
AU (1) AU2010240473B2 (en)
BR (1) BRPI1006628B8 (en)
CA (1) CA2759405C (en)
DK (1) DK2421963T4 (en)
ES (1) ES2616057T5 (en)
GB (1) GB0908770D0 (en)
NZ (1) NZ595833A (en)
WO (1) WO2010122531A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102604913A (en) * 2012-04-05 2012-07-25 湖南尤特尔生化有限公司 Preparation method and application of thermomyces lanuginosus lipase

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0908770D0 (en) * 2009-04-24 2009-07-01 Danisco Method
US8759044B2 (en) 2011-03-23 2014-06-24 Butamax Advanced Biofuels Llc In situ expression of lipase for enzymatic production of alcohol esters during fermentation
US8765425B2 (en) 2011-03-23 2014-07-01 Butamax Advanced Biofuels Llc In situ expression of lipase for enzymatic production of alcohol esters during fermentation
EP2758514B2 (en) 2011-09-22 2020-03-18 Danisco US Inc. Endogenous dnase activity to reduce dna content
AP2015008957A0 (en) * 2013-06-27 2015-12-31 Sasol Tech Pty Ltd Production of biomass for use in the treatment of fischer-tropsch reaction water
CN106434597B (en) * 2016-11-08 2019-10-01 湖南尤特尔生化有限公司 A kind of chaetomium thermophilum phosphatidase and its preparation method and application

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4797361A (en) * 1983-10-24 1989-01-10 Lehigh University Microorganism and process
WO1998045453A1 (en) * 1997-04-09 1998-10-15 Danisco A/S Lipase and use of same for improving doughs and baked products
WO2004101759A2 (en) * 2003-05-12 2004-11-25 Genencor International, Inc. Novel lipolytic enzyme lip2
WO2006077258A1 (en) * 2005-01-24 2006-07-27 Dsm Ip Assets B.V. Method for producing a compound of interest in a filamentous fungal cell
WO2008150376A1 (en) * 2007-05-21 2008-12-11 Danisco Us, Inc., Genencor Division Use of an aspartic protease (nsp24) signal sequence for heterologous protein expression
US20090269805A1 (en) * 2003-05-12 2009-10-29 Jones Brian E Novel Lipolytic Enzyme ELIP

Family Cites Families (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL154598B (en) 1970-11-10 1977-09-15 Organon Nv PROCEDURE FOR DETERMINING AND DETERMINING LOW MOLECULAR COMPOUNDS AND PROTEINS THAT CAN SPECIFICALLY BIND THESE COMPOUNDS AND TEST PACKAGING.
US3817837A (en) 1971-05-14 1974-06-18 Syva Corp Enzyme amplification assay
US3939350A (en) 1974-04-29 1976-02-17 Board Of Trustees Of The Leland Stanford Junior University Fluorescent immunoassay employing total reflection for activation
US3996345A (en) 1974-08-12 1976-12-07 Syva Company Fluorescence quenching with immunological pairs in immunoassays
US4275149A (en) 1978-11-24 1981-06-23 Syva Company Macromolecular environment control in specific receptor assays
US4277437A (en) 1978-04-05 1981-07-07 Syva Company Kit for carrying out chemically induced fluorescence immunoassay
US4366241A (en) 1980-08-07 1982-12-28 Syva Company Concentrating zone method in heterogeneous immunoassays
US4816567A (en) 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
EP0590689B2 (en) 1985-03-30 2006-08-16 KAUFFMAN, Stuart A. Method for obtaining DNA, RNA, peptides, polypeptides or proteins by means of a DNA-recombinant technique
US5780292A (en) 1987-04-29 1998-07-14 Alko Group Ltd. Production of phytate degrading enzymes in trichoderma
AU639570B2 (en) 1990-05-09 1993-07-29 Novozymes A/S A cellulase preparation comprising an endoglucanase enzyme
DE4112440C1 (en) 1991-04-16 1992-10-22 Diagen Institut Fuer Molekularbiologische Diagnostik Gmbh, 4000 Duesseldorf, De
EP0673429B1 (en) 1992-12-10 2002-06-12 Dsm N.V. Production of heterologous proteins in filamentous fungi
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5741665A (en) 1994-05-10 1998-04-21 University Of Hawaii Light-regulated promoters for production of heterologous proteins in filamentous fungi
GB9821198D0 (en) 1998-09-30 1998-11-25 Danisco Enzyme
CN1181197C (en) † 1999-01-25 2004-12-22 诺维信公司 Recovery of a protein at high pH
MXPA00011569A (en) 1999-03-26 2004-12-03 Diversa Corp Exonuclease-mediated nucleic acid reassembly in directed evolution.
DK1862626T3 (en) 2003-05-29 2011-12-12 Genencor Int New Trichoderma Genes
US7740307B2 (en) 2006-06-14 2010-06-22 Guido Benvenuto Motor vehicle component and methods for its manufacture
US20080026376A1 (en) 2006-07-11 2008-01-31 Huaming Wang KEX2 cleavage regions of recombinant fusion proteins
CN103451164A (en) * 2006-07-14 2013-12-18 诺维信股份有限公司 Methods for producing secreted polypeptides having biological activity
EP2132308B1 (en) 2007-03-14 2011-11-16 Danisco US Inc. Trichoderma reese a-amylase enhances saccharification of corn starch
DK2152892T3 (en) 2007-05-21 2013-07-22 Danisco Us Inc Genencor Div Method of introducing nucleic acids into fungal cells
FI120835B (en) † 2007-07-10 2010-03-31 Valtion Teknillinen New esters and their use
GB0908770D0 (en) * 2009-04-24 2009-07-01 Danisco Method

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4797361A (en) * 1983-10-24 1989-01-10 Lehigh University Microorganism and process
WO1998045453A1 (en) * 1997-04-09 1998-10-15 Danisco A/S Lipase and use of same for improving doughs and baked products
WO2004101759A2 (en) * 2003-05-12 2004-11-25 Genencor International, Inc. Novel lipolytic enzyme lip2
US20090269805A1 (en) * 2003-05-12 2009-10-29 Jones Brian E Novel Lipolytic Enzyme ELIP
WO2006077258A1 (en) * 2005-01-24 2006-07-27 Dsm Ip Assets B.V. Method for producing a compound of interest in a filamentous fungal cell
WO2008150376A1 (en) * 2007-05-21 2008-12-11 Danisco Us, Inc., Genencor Division Use of an aspartic protease (nsp24) signal sequence for heterologous protein expression

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
BRADNER J RON ET AL: "The application of PCR for the isolation of a lipase gene from the genomic DNA of an Antarctic microfungus.", CURRENT GENETICS, vol. 44, no. 4, December 2003 (2003-12-01), pages 224 - 230, XP002602992, ISSN: 0172-8083 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102604913A (en) * 2012-04-05 2012-07-25 湖南尤特尔生化有限公司 Preparation method and application of thermomyces lanuginosus lipase

Also Published As

Publication number Publication date
BRPI1006628B1 (en) 2019-04-16
CA2759405A1 (en) 2010-10-28
CN102439143A (en) 2012-05-02
EP2421963B1 (en) 2016-11-30
AU2010240473B2 (en) 2014-01-30
DK2421963T3 (en) 2017-03-06
US9382523B2 (en) 2016-07-05
AU2010240473A1 (en) 2011-11-10
US20120196002A1 (en) 2012-08-02
ES2616057T3 (en) 2017-06-09
GB0908770D0 (en) 2009-07-01
CA2759405C (en) 2020-07-21
EP2421963B2 (en) 2022-12-28
US20150064309A1 (en) 2015-03-05
US8940519B2 (en) 2015-01-27
CN102439143B (en) 2016-03-09
WO2010122531A2 (en) 2010-10-28
BRPI1006628B8 (en) 2019-05-14
EP2421963A2 (en) 2012-02-29
BRPI1006628A2 (en) 2015-08-25
ES2616057T5 (en) 2023-05-23
NZ595833A (en) 2013-10-25
DK2421963T4 (en) 2023-03-27

Similar Documents

Publication Publication Date Title
WO2011056644A3 (en) Anti-glp-1r antibodies and their uses
WO2011061032A3 (en) Cells, nucleic acids, enzymes, and use thereof, and methods for the production of sophorolipids
WO2013021279A3 (en) Highly galactosylated antibodies
HK1202308A1 (en) Water soluble compositions incorporating enzymes, and method of making same
MX2012007710A (en) Alpha-amylases.
EP2694269A4 (en) Lignin compositions, methods of producing the compositions, methods of using lignin compositions, and products produced thereby
WO2012138981A3 (en) New intermediates and processes for preparing ticagrelor
PL2748300T3 (en) Enzyme producing bacillus strains
WO2013087073A3 (en) Substrate with a structured surface and methods for the production thereof, and methods for determining the wetting properties thereof
WO2012106407A3 (en) Diagnostic and therapeutic methods and products related to anxiety disorders
WO2011060126A3 (en) Low-carbon fertilizers and processes for producing the same
WO2012061603A3 (en) Compositions comprising and methods for forming functionalized carbon-based nanostructures
EP2520288A4 (en) Production method of solid preparation and the preparation produced by the method
WO2010122531A3 (en) Method of producing a lipolytic enzyme
WO2010123728A3 (en) Method of making and using activated carbon-containing coated substrates and the products made therefrom
WO2011022548A3 (en) Ketoreductase polypeptides for the preparation of phenylephrine
WO2011133504A3 (en) Nanozymes, methods of making nanozymes, and methods of using nanozymes
EP2599848A4 (en) Water-and-oil repellant composition, functional textile product, and production method for functional textile product
WO2010110599A3 (en) Novel αlpha-neoagarobiose hydrolase, and method for obtaining a monosaccharide using same
EP2513241A4 (en) Adhesive, method of producing the same and uses thereof
WO2010009879A3 (en) Methods for producing paricalcitol
TWI563089B (en) Thermostable xylanase, production method and use thereof, and method for producing lignocellulosic glycation product
MX344909B (en) Methods for producing polyetherols.
WO2014048776A3 (en) Water-emulsible isocyanates having improved gloss
WO2012136898A3 (en) Novel cutinases, their production and uses

Legal Events

Date Code Title Description
WWE Wipo information: entry into national phase

Ref document number: 201080018179.3

Country of ref document: CN

121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 10723329

Country of ref document: EP

Kind code of ref document: A2

WWE Wipo information: entry into national phase

Ref document number: 595833

Country of ref document: NZ

WWE Wipo information: entry into national phase

Ref document number: 2759405

Country of ref document: CA

WWE Wipo information: entry into national phase

Ref document number: 8209/DELNP/2011

Country of ref document: IN

NENP Non-entry into the national phase

Ref country code: DE

ENP Entry into the national phase

Ref document number: 2010240473

Country of ref document: AU

Date of ref document: 20100423

Kind code of ref document: A

REEP Request for entry into the european phase

Ref document number: 2010723329

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 2010723329

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 13265712

Country of ref document: US

REG Reference to national code

Ref country code: BR

Ref legal event code: B01A

Ref document number: PI1006628

Country of ref document: BR

ENP Entry into the national phase

Ref document number: PI1006628

Country of ref document: BR

Kind code of ref document: A2

Effective date: 20111021