WO2011011463A3 - Manipulation of an alternative respiratory pathway in photo-autotrophs - Google Patents

Manipulation of an alternative respiratory pathway in photo-autotrophs Download PDF

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Publication number
WO2011011463A3
WO2011011463A3 PCT/US2010/042666 US2010042666W WO2011011463A3 WO 2011011463 A3 WO2011011463 A3 WO 2011011463A3 US 2010042666 W US2010042666 W US 2010042666W WO 2011011463 A3 WO2011011463 A3 WO 2011011463A3
Authority
WO
WIPO (PCT)
Prior art keywords
algal cell
methods
aox gene
seq
amino acid
Prior art date
Application number
PCT/US2010/042666
Other languages
French (fr)
Other versions
WO2011011463A2 (en
Inventor
Shaun Bailey
Bertrand Vick
Jeff Moseley
Original Assignee
Aurora Biofuels, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aurora Biofuels, Inc. filed Critical Aurora Biofuels, Inc.
Publication of WO2011011463A2 publication Critical patent/WO2011011463A2/en
Publication of WO2011011463A3 publication Critical patent/WO2011011463A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6463—Glycerides obtained from glyceride producing microorganisms, e.g. single cell oil

Abstract

Exemplary methods for increasing TAG production in an algal cell during imbalanced growth conditions are provided. Some methods comprise knocking out an AOX gene, wherein the AOX gene produces an amino acid sequence having substantial similarity to the amino acid sequence of SEQ. ID. NO. 2. In further methods, the algal cell may be of genus Nannochloropsis. The AOX gene may be replaced by a construct having a nucleotide sequence having substantial similarity to SEQ ID. NOS. 3 through 5 (inclusive), wherein each of the sequences are next to or in close proximity to one another in a linear fashion. In some methods, the AOX gene may be replaced via homologous recombination. As a result, lipid production by the selected recombinant algal cell may be increased over that produced by a wild-type algal cell.
PCT/US2010/042666 2009-07-20 2010-07-20 Manipulation of an alternative respiratory pathway in photo-autotrophs WO2011011463A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US22702609P 2009-07-20 2009-07-20
US61/227,026 2009-07-20

Publications (2)

Publication Number Publication Date
WO2011011463A2 WO2011011463A2 (en) 2011-01-27
WO2011011463A3 true WO2011011463A3 (en) 2011-10-06

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2010/042666 WO2011011463A2 (en) 2009-07-20 2010-07-20 Manipulation of an alternative respiratory pathway in photo-autotrophs

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US (1) US8709765B2 (en)
WO (1) WO2011011463A2 (en)

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WO2011011463A2 (en) 2011-01-27
US20110059495A1 (en) 2011-03-10
US8709765B2 (en) 2014-04-29

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