WO2011011463A3 - Manipulation of an alternative respiratory pathway in photo-autotrophs - Google Patents
Manipulation of an alternative respiratory pathway in photo-autotrophs Download PDFInfo
- Publication number
- WO2011011463A3 WO2011011463A3 PCT/US2010/042666 US2010042666W WO2011011463A3 WO 2011011463 A3 WO2011011463 A3 WO 2011011463A3 US 2010042666 W US2010042666 W US 2010042666W WO 2011011463 A3 WO2011011463 A3 WO 2011011463A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- algal cell
- methods
- aox gene
- seq
- amino acid
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6436—Fatty acid esters
- C12P7/6445—Glycerides
- C12P7/6463—Glycerides obtained from glyceride producing microorganisms, e.g. single cell oil
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Cell Biology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Botany (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Exemplary methods for increasing TAG production in an algal cell during imbalanced growth conditions are provided. Some methods comprise knocking out an AOX gene, wherein the AOX gene produces an amino acid sequence having substantial similarity to the amino acid sequence of SEQ. ID. NO. 2. In further methods, the algal cell may be of genus Nannochloropsis. The AOX gene may be replaced by a construct having a nucleotide sequence having substantial similarity to SEQ ID. NOS. 3 through 5 (inclusive), wherein each of the sequences are next to or in close proximity to one another in a linear fashion. In some methods, the AOX gene may be replaced via homologous recombination. As a result, lipid production by the selected recombinant algal cell may be increased over that produced by a wild-type algal cell.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US22702609P | 2009-07-20 | 2009-07-20 | |
US61/227,026 | 2009-07-20 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2011011463A2 WO2011011463A2 (en) | 2011-01-27 |
WO2011011463A3 true WO2011011463A3 (en) | 2011-10-06 |
Family
ID=43499638
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2010/042666 WO2011011463A2 (en) | 2009-07-20 | 2010-07-20 | Manipulation of an alternative respiratory pathway in photo-autotrophs |
Country Status (2)
Country | Link |
---|---|
US (1) | US8709765B2 (en) |
WO (1) | WO2011011463A2 (en) |
Families Citing this family (14)
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EP2297326A4 (en) * | 2008-06-06 | 2011-11-16 | Aurora Biofuels Inc | Vcp-based vectors for algal cell transformation |
US20100022393A1 (en) * | 2008-07-24 | 2010-01-28 | Bertrand Vick | Glyphosate applications in aquaculture |
US8314228B2 (en) | 2009-02-13 | 2012-11-20 | Aurora Algae, Inc. | Bidirectional promoters in Nannochloropsis |
US9029137B2 (en) | 2009-06-08 | 2015-05-12 | Aurora Algae, Inc. | ACP promoter |
US8865468B2 (en) | 2009-10-19 | 2014-10-21 | Aurora Algae, Inc. | Homologous recombination in an algal nuclear genome |
US8809046B2 (en) | 2011-04-28 | 2014-08-19 | Aurora Algae, Inc. | Algal elongases |
WO2011011463A2 (en) | 2009-07-20 | 2011-01-27 | Aurora Biofuels, Inc. | Manipulation of an alternative respiratory pathway in photo-autotrophs |
US8722359B2 (en) | 2011-01-21 | 2014-05-13 | Aurora Algae, Inc. | Genes for enhanced lipid metabolism for accumulation of lipids |
JP2014519810A (en) | 2011-04-28 | 2014-08-21 | オーロラ アルギー,インコーポレイテッド | Algal desaturase |
WO2013188462A2 (en) | 2012-06-12 | 2013-12-19 | Synthetic Genomics, Inc. | Regulatory elements and uses thereof |
WO2014089348A1 (en) | 2012-12-07 | 2014-06-12 | Synthetic Genomics, Inc. | Nannochloropsis spliced leader sequences and uses therefor |
US10123986B2 (en) | 2012-12-24 | 2018-11-13 | Qualitas Health, Ltd. | Eicosapentaenoic acid (EPA) formulations |
US9629820B2 (en) | 2012-12-24 | 2017-04-25 | Qualitas Health, Ltd. | Eicosapentaenoic acid (EPA) formulations |
CN113736797B (en) * | 2020-05-27 | 2023-08-15 | 中国科学院青岛生物能源与过程研究所 | Culture method for improving yield of microalgae Triglyceride (TAG) and application thereof |
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Also Published As
Publication number | Publication date |
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US20110059495A1 (en) | 2011-03-10 |
US8709765B2 (en) | 2014-04-29 |
WO2011011463A2 (en) | 2011-01-27 |
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