WO2011049669A3 - Genetic fingerprinting and identification method - Google Patents

Genetic fingerprinting and identification method Download PDF

Info

Publication number
WO2011049669A3
WO2011049669A3 PCT/US2010/046004 US2010046004W WO2011049669A3 WO 2011049669 A3 WO2011049669 A3 WO 2011049669A3 US 2010046004 W US2010046004 W US 2010046004W WO 2011049669 A3 WO2011049669 A3 WO 2011049669A3
Authority
WO
WIPO (PCT)
Prior art keywords
organism
sample
nucleic acid
labeled
mix
Prior art date
Application number
PCT/US2010/046004
Other languages
French (fr)
Other versions
WO2011049669A9 (en
WO2011049669A2 (en
Inventor
Frank R. Burns
Xuan PENG
Original Assignee
E.I. Dupont De Nemours And Company
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by E.I. Dupont De Nemours And Company filed Critical E.I. Dupont De Nemours And Company
Priority to BR112012003640A priority Critical patent/BR112012003640A2/en
Priority to JP2012525685A priority patent/JP2013502222A/en
Priority to EP10813092A priority patent/EP2467501A2/en
Priority to AU2010308516A priority patent/AU2010308516A1/en
Priority to MX2012002101A priority patent/MX2012002101A/en
Priority to CA2769825A priority patent/CA2769825A1/en
Publication of WO2011049669A2 publication Critical patent/WO2011049669A2/en
Publication of WO2011049669A9 publication Critical patent/WO2011049669A9/en
Publication of WO2011049669A3 publication Critical patent/WO2011049669A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
    • G16B20/20—Allele or variant detection, e.g. single nucleotide polymorphism [SNP] detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Theoretical Computer Science (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Evolutionary Biology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Medical Informatics (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present disclosure provides methods for molecular fingerprinting for the characterization and identification of organisms. More specifically, in one aspect the present invention provides a method of identifying an organism in a sample comprising: (a) providing a sample comprising said organism, said organism comprising at least one nucleic acid; (b) combining said sample or the at least one nucleic acid therefrom with an amplification mix comprising at least one labeled oligonucleotide primer; (c) generating at least one labeled amplification product from the at least one nucleic acid of said organism using a nucleotide amplification technique employing said at least one labeled oligonucleotide primer; (d) combining said at least one labeled amplification product with products of a DNA sequencing reaction to create a separation mix; and (e) separating said separation mix on the basis of oligonucleotide length in a fluorescent DNA sequencing instrument to generate a sequence embedded fingerprint pattern for said organism.
PCT/US2010/046004 2009-08-21 2010-08-19 Genetic fingerprinting and identification method WO2011049669A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
BR112012003640A BR112012003640A2 (en) 2009-08-21 2010-08-19 organism identification method in an isolated polynucleotide sample and primer comprising isolated polynucleotide
JP2012525685A JP2013502222A (en) 2009-08-21 2010-08-19 Gene fingerprinting and identification method
EP10813092A EP2467501A2 (en) 2009-08-21 2010-08-19 Genetic fingerprinting and identification method
AU2010308516A AU2010308516A1 (en) 2009-08-21 2010-08-19 Genetic fingerprinting and identification method
MX2012002101A MX2012002101A (en) 2009-08-21 2010-08-19 Genetic fingerprinting and identification method.
CA2769825A CA2769825A1 (en) 2009-08-21 2010-08-19 Genetic fingerprinting and identification method

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US23599909P 2009-08-21 2009-08-21
US61/235,999 2009-08-21

Publications (3)

Publication Number Publication Date
WO2011049669A2 WO2011049669A2 (en) 2011-04-28
WO2011049669A9 WO2011049669A9 (en) 2011-06-16
WO2011049669A3 true WO2011049669A3 (en) 2011-08-04

Family

ID=43605665

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2010/046004 WO2011049669A2 (en) 2009-08-21 2010-08-19 Genetic fingerprinting and identification method

Country Status (8)

Country Link
US (2) US8481267B2 (en)
EP (1) EP2467501A2 (en)
JP (1) JP2013502222A (en)
AU (1) AU2010308516A1 (en)
BR (1) BR112012003640A2 (en)
CA (1) CA2769825A1 (en)
MX (1) MX2012002101A (en)
WO (1) WO2011049669A2 (en)

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4879214A (en) * 1988-11-15 1989-11-07 E. I. Du Pont De Nemours And Company Differentiation of nucleic acid segments on the basis of nucleotide differences
US5126239A (en) * 1990-03-14 1992-06-30 E. I. Du Pont De Nemours And Company Process for detecting polymorphisms on the basis of nucleotide differences
EP0721987A1 (en) * 1995-01-16 1996-07-17 Keygene N.V. Amplification of simple sequence repeats
US6263286B1 (en) * 1998-08-13 2001-07-17 U.S. Genomics, Inc. Methods of analyzing polymers using a spatial network of fluorophores and fluorescence resonance energy transfer

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
BENECKE M: "Random amplified polymorphic DNA (RAPD) typing of necrophageous insects (Diptera, Coleoptera) in criminal forensic studies: validation and use in practice.", FORENSIC SCIENCE INTERNATIONAL 21 DEC 1998 LNKD- PUBMED:9924784, vol. 98, no. 3, 21 December 1998 (1998-12-21), pages 157 - 168, XP002632236, ISSN: 0379-0738 *
HEALY M ET AL: "Use of the Diversi Lab System for species and strain differentiation of Fusarium species isolates.", JOURNAL OF CLINICAL MICROBIOLOGY OCT 2005 LNKD- PUBMED:16207996, vol. 43, no. 10, October 2005 (2005-10-01), pages 5278 - 5280, XP002632237, ISSN: 0095-1137 *
HEALY MIMI ET AL: "Microbial DNA typing by automated repetitive-sequence-based PCR.", JOURNAL OF CLINICAL MICROBIOLOGY JAN 2005 LNKD- PUBMED:15634972, vol. 43, no. 1, January 2005 (2005-01-01), pages 199 - 207, XP002632235, ISSN: 0095-1137 *
HILTON A C ET AL: "Optimization of RAPD for fingerprinting Salmonella.", LETTERS IN APPLIED MICROBIOLOGY APR 1997 LNKD- PUBMED:9134770, vol. 24, no. 4, April 1997 (1997-04-01), pages 243 - 248, XP002632241, ISSN: 0266-8254 *
HILTON A C ET AL: "Random amplification of polymorphic DNA (RAPD) of Salmonella: strain differentiation and characterization of amplified sequences.", THE JOURNAL OF APPLIED BACTERIOLOGY DEC 1996 LNKD- PUBMED:8972084, vol. 81, no. 6, December 1996 (1996-12-01), pages 575 - 584, XP002632242, ISSN: 0021-8847 *
KUSKE C R ET AL: "Small-scale DNA sample preparation method for field PCR detection of microbial cells and spores in soil", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 64, no. 7, 1 July 1998 (1998-07-01), pages 2463 - 2472, XP002226433, ISSN: 0099-2240 *
POUNDER JUNE I ET AL: "Clinical evaluation of repetitive sequence-based polymerase chain reaction using the Diversi-Lab System for strain typing of vancomycin-resistant enterococci.", DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE MAR 2006 LNKD- PUBMED:16423495, vol. 54, no. 3, March 2006 (2006-03-01), pages 183 - 187, XP002632239, ISSN: 0732-8893 *
POUNDER JUNE I ET AL: "Repetitive-sequence-PCR-based DNA fingerprinting using the Diversilab system for identification of commonly encountered dermatophytes.", JOURNAL OF CLINICAL MICROBIOLOGY MAY 2005 LNKD- PUBMED:15872233, vol. 43, no. 5, May 2005 (2005-05-01), pages 2141 - 2147, XP002632238, ISSN: 0095-1137 *
SHUTT CHERYL K ET AL: "Clinical evaluation of the DiversiLab microbial typing system using repetitive-sequence-based PCR for characterization of Staphylococcus aureus strains.", JOURNAL OF CLINICAL MICROBIOLOGY MAR 2005 LNKD- PUBMED:15750081, vol. 43, no. 3, March 2005 (2005-03-01), pages 1187 - 1192, XP002632240, ISSN: 0095-1137 *

Also Published As

Publication number Publication date
JP2013502222A (en) 2013-01-24
US20110045478A1 (en) 2011-02-24
BR112012003640A2 (en) 2017-07-18
US8697852B2 (en) 2014-04-15
WO2011049669A9 (en) 2011-06-16
WO2011049669A2 (en) 2011-04-28
AU2010308516A1 (en) 2012-02-02
US8481267B2 (en) 2013-07-09
CA2769825A1 (en) 2011-04-28
EP2467501A2 (en) 2012-06-27
US20130274458A1 (en) 2013-10-17
MX2012002101A (en) 2012-04-11

Similar Documents

Publication Publication Date Title
WO2008011565A3 (en) Method for identifying novel genes
AU2014204426B2 (en) Integrated nucleic acid analysis
WO2007123744A3 (en) Systems and devices for sequence by synthesis analysis
WO2012162267A3 (en) Nucleic acid encoding reactions
WO2006033020A3 (en) Methods and apparatus for the detection and validation of micrornas
WO2005089508A3 (en) Dna sequence detection by limited primer extension
WO2010026488A3 (en) Methods and kits for nucleic acid sequencing
WO2008115185A3 (en) Use of microarrays for genomic representation selection
WO2010068884A3 (en) Methods and systems for direct sequencing of single dna molecules
WO2004039953A3 (en) Array oligomer synthesis and use.
UA92720C2 (en) Method for simulteneous identification of mycobacteria of tuberculosis complex and identification of mutations of dna of mycobacteria, leading to resistance of microorganisms towards rifampicin and isoniazid, on biological microchips, set of primers, biochip, and set of oligonucleotide probes, used in a method
HK1130844A1 (en) Methods and devices for detection and identification of encoded beads and biological molecules
AU2010315303A8 (en) Quantitative Nuclease Protection Sequencing (qNPS)
NZ610129A (en) Systems and methods for automated reusable parallel biological reactions
WO2008051530A3 (en) Polymerase enzymes and reagents for enhanced nucleic acid sequencing
EP2044191A4 (en) Systems and methods for monitoring the amplification and dissociation behavior of dna molecules
WO2007041342A3 (en) Labeled nucleotide analogs and uses therefor
WO2005113822A3 (en) Dna profiling and snp detection utilizing microarrays
WO2008122002A3 (en) Organism identification panel
WO2011140187A3 (en) Detecting chromosomal rearrangement
WO2009116863A3 (en) Expression-linked gene discovery
WO2010075414A3 (en) Method for detection of xmrv
AU2009355009A8 (en) THD primer target detection
WO2004027024A3 (en) Isolation of genetic molecules from a complex biological construct for use in genetic expression analysis
ATE493517T1 (en) CONTROL GENES FOR NORMALIZING GENE EXPRESSION ANALYSIS DATA

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 10813092

Country of ref document: EP

Kind code of ref document: A1

WWE Wipo information: entry into national phase

Ref document number: 2010308516

Country of ref document: AU

WWE Wipo information: entry into national phase

Ref document number: 2769825

Country of ref document: CA

ENP Entry into the national phase

Ref document number: 2010308516

Country of ref document: AU

Date of ref document: 20100819

Kind code of ref document: A

WWE Wipo information: entry into national phase

Ref document number: MX/A/2012/002101

Country of ref document: MX

WWE Wipo information: entry into national phase

Ref document number: 2012525685

Country of ref document: JP

NENP Non-entry into the national phase

Ref country code: DE

WWE Wipo information: entry into national phase

Ref document number: 2010813092

Country of ref document: EP

REG Reference to national code

Ref country code: BR

Ref legal event code: B01A

Ref document number: 112012003640

Country of ref document: BR

ENP Entry into the national phase

Ref document number: 112012003640

Country of ref document: BR

Kind code of ref document: A2

Effective date: 20120217