WO2011056598A2 - Membrane filter system and apparatus - Google Patents

Membrane filter system and apparatus Download PDF

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Publication number
WO2011056598A2
WO2011056598A2 PCT/US2010/054138 US2010054138W WO2011056598A2 WO 2011056598 A2 WO2011056598 A2 WO 2011056598A2 US 2010054138 W US2010054138 W US 2010054138W WO 2011056598 A2 WO2011056598 A2 WO 2011056598A2
Authority
WO
WIPO (PCT)
Prior art keywords
membrane
filter
frame
void
area
Prior art date
Application number
PCT/US2010/054138
Other languages
French (fr)
Other versions
WO2011056598A3 (en
Inventor
Triantafyllos P. Tafas
Original Assignee
Ikonisys, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ikonisys, Inc. filed Critical Ikonisys, Inc.
Publication of WO2011056598A2 publication Critical patent/WO2011056598A2/en
Publication of WO2011056598A3 publication Critical patent/WO2011056598A3/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4005—Concentrating samples by transferring a selected component through a membrane
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D63/00—Apparatus in general for separation processes using semi-permeable membranes
    • B01D63/08—Flat membrane modules
    • B01D63/087—Single membrane modules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D69/00—Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or properties; Manufacturing processes specially adapted therefor
    • B01D69/10—Supported membranes; Membrane supports
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/34—Microscope slides, e.g. mounting specimens on microscope slides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2313/00—Details relating to membrane modules or apparatus
    • B01D2313/02—Specific tightening or locking mechanisms
    • B01D2313/025—Specific membrane holders

Definitions

  • a common method for performing separation is to pass a fluid or fluid-like sample containing the desired target through a filter element having one or more pass- through apertures of a select dimension. By appropriate selection of the filter, one may preferentially obtain targeted objects on the filter while the residual fluid and other residues pass through the filter and are removed.
  • An apparatus may be employed that faci litates the inputting of the fluid sample, the passing of the sample through the filter, and the removal of the residual fluid.
  • the design of the filter element and the collection apparatus may provide for the safe removal of the filtered material from the filter, and its subsequent mounting on a substrate suitable for further necessary processing and microscopic examination.
  • the filter element and collection apparatus should not introduce any artifacts into the collected sample.
  • the distribution of the collected targeted cells should be uniform across the surface area of the filter.
  • a membrane fi lter and membrane filter apparatus for facilitating the preparation of a microscope slide containing a filtered sample.
  • the filter material having defined tillering characteristics (such as porosity, charge, lipophilicity, etc. depending on the substrate desired to be preferentially filtered out) is mounted in a transport frame. After filtration, the loaded filter material is removed from the frame and adhesively bonded to a microscope slide.
  • the frame comprises upper and lower frame portions, that are configured to articulate with one another so as to connect to one another at least one portion of the same.
  • the upper and lower frame portions are configured to press-fit with one another.
  • at least the upper or lower frame portion have an adhesive area associated therewith that allows for fixed articulation between both frame portions.
  • the frame may be of any shape, including rectangular, and may comprise multiple portions and typically defines a void between frame portions.
  • Such frame embodiments are designed to house there-between a membrane that my span between portions of the frame.
  • Such membrane may comprise delimiters that mark a selected area for placement in the void between frame portions.
  • Such membrane may further comprise a data recordation area which may be interrogated for information pertaining to the attributes of the slide and/or sample, and/or interrogated for storage of information onto the data recordation area.
  • the membrane may further comprise a filtration demarcation area designed to instruct a user to where sample should be placed for filtration (and where the fi ltered sample exists after application of filtration).
  • the filtration demarcation area may be fabricated identical to the rest of the membrane, or may have special fi ltration characteristics unique to the membrane.
  • the membrane having the filtration demarcation area and the data recordation area is placed between the upper and lower frame portions so as to course between frame elements.
  • the framed delimited membrane may be applied to a suction apparatus to allow for quicker filtration through the membrane.
  • Such suction apparatus may comprise a vessel with a vacuum conduit running there through and an upper sealed area surrounding a void of approximately the geometry of the filtration demarcation area of the membrane.
  • the frame and upper portion of the suction apparatus are configured to allow the framed membrane to lie on top of the vessel such that the filtration demarcation area is positioned over a portion of the vacuum conduit.
  • the framed membrane may be removed from the frame after filtration, and the membrane affixed to a more solid substrate for review under a microscope.
  • the membrane itself may be selected to be sufficient turgidity such that the membrane itsel f may be used as the slide itself.
  • the microscope slide may comprise any substrate, but often preferentially glass or clear polymer.
  • the microscope slide may be manufactured to have portions thereof that have activatable adhesive thereon such that the membrane adheres to the slide when applied.
  • the slide has adhesive portions that are remote from the portion over which the filtration demarcation area of the membrane will reside.
  • Fig. 1 is a perspective drawing of the filter transport frame. A double sided adhesive is applied to the molded filter membrane tension/transportation frame.
  • Fig. 2 portrays the mounting of the filter membrane.
  • the molded filter membrane tension'transportation frame is applied to the preprinted (barcode and deposition area) filter membrane.
  • the membrane may be cut from a continuous roll.
  • Fig. 3 displays the completed filter element.
  • the complete assembly comprises a molded filter transportation frame applied to the preprinted filter membrane.
  • Fig. 4 illustrates the mounting of the fi lter element in a suitable collection apparatus.
  • Fig. 5 illustrates the removal of the membrane after filtration and placement into an alignment fixture for transfer of the membrane to the sl ide.
  • the membrane is then trimmed from the frame, just inside the frame edges, using a heated knife edge.
  • a membrane filter for separating and collecting structures contained in a fluid sample, such as cells, on the basis of their size.
  • the design of membrane filter facilitates its safe insertion and removal from the collection apparatus and minimizes the risk of introduction of artifacts into the collected sample.
  • the advantages of the embodiments include 1 ) the elimination of the need for manual membrane handling thereby insuring sample integrity; and 2) the flatness of the filter element and the subsequent slide is suitable for automated microscope examination.
  • the cell distribution on the membrane surface is optimized for imaging; and (2) the filter is suitable for IF and FISH processing and staining protocols and is compatible with auto-staining systems;
  • the fi lter material is manufactured as a strip or ribbon of suitable material such as TEM i.e., track etched membrane.
  • the strip may comprise fi lter sections having porosity properties that permit the penetration of fluids and structures having less than a specified dimension. Structures larger than the specified dimension wi l l be captured by the filter material.
  • non-filtering sections may be placed adjacent to the filtering sections. The non-filtering sections may provide an area for inclusion of data media such as textual or bar-code identification markings, or other magnetically, optically, or electronically readable and/or writable media.
  • a single use filter element is manufactured by adhesively mounting a section of the filter material into a transportation frame.
  • Other non-adhesive mounting methods such as snap fit. may alternatively be employed.
  • the transportation frame may be rectangular and substantially planar or, alternatively, it may be non-rectangular and substantially planar.
  • the filter material may be stretched to insure that flatness is maintained when mounted in the rigid transportation frame.
  • the transport frame may be designed for one use. or mav allow for new membrane to be added to the frame so as to course across the framed void .
  • the frame has a void in each of two parallel sections allowing new membrane to be added bv removing the filtered membrane from the frame through one void, and adding new membrane through the other void.
  • the transportation frame containing the filter element, may be designed to be loaded into a collection apparatus.
  • the collection apparatus may comprise an input reservoir, a filter element holder, and a discharge receptacle.
  • the apparatus may be configured so that sample fluid loaded into a an input reservoir, containing the targeted cells, is made to pass through the filter material, and then to a discharge receptacle. The process may be effectuated by applying a positive pressure to the input reservoir and/or a negative pressure to the discharge receptacle.
  • the transportation frame containing the filter element charged with the collected cells, is removed from the collection apparatus.
  • the transportation frame may be brought into planar contact with an adhesively coated microscope slide using a guiding template.
  • the filter element adheres to the surface of the microscope slide.
  • the filter element material may be cut to release it from the transport frame.
  • FIG. 1 there is shown a frame that may find use in the present disclosure.
  • Frame 10 comprises two parts an upper frame portion 20 and a lower frame portion 30.
  • Upper frame portion 20 is shown to have adhesive portions thereon 20 which may be used to affix upper frame portion 20 to lower frame portion 30.
  • the frame defines a void 25.
  • membrane 10 is designed to have a delimited area set forth by delimiters 70 such that the delimited membrane may fit within void 25 of frame 10 of Fig. /.
  • Membrane 10, as illustrated, comprises a filtration demarcation area 60 and a data recordation area 50.
  • FIG. 5 there is shown a adjoined frame with a fixed membrane 80 there between, coursing through void 25 between parallel legs of the frame.
  • a suction support apparatus such as shown in Fig. 4A as 120.
  • Apparatus 120 as shown comprise suction support structure 1 10 having a top portion 125 and bottom portion 130. Top portion has gasket 1 15 which defines a void area through which vacuum conduit 90 terminates. Vacuum conduit 90 terminates on other end in vacuum port 120 to which a vacuum may be affixed to allow for the formation of a vacuum throughout v acuum conduit 90.
  • Framed membrane 80 is placed such that vacuum conduit 90 is positioned beneath filtration demarcation area 60.
  • Fig 48 a top view of the framed membrane 80 sitting on the top gasketed portion of suction support structure 1 10.
  • framed membrane 80 may be excised from frame 20 and then placed on a substrate, such as microscope slide 120.
  • microscope slide 1 20 has adhesive portions 130 which allow for fixation of membrane 40 thereon with portion 120. which lacks such adhesive, providing a corresponding area against which filtration demarcation area 60 of membrane 40 may rest.

Abstract

In embodiments there is disclosed, a filter apparatus comprising a substantially planar frame defining a void and a filter material in the form of a sheet fastened to said substantially plane course through said void, wherein said filter material comprises at least one data recordation area and a filtration demarcation area.

Description

MEMBRANE FILTER SYSTEM AND APPARATUS
BACKGROUND
[ 0000 1] In the field of microscopy, it is sometimes necessary to separate and collect targeted objects such as biological structures (e.g. cells) on the basis of a physicochemical property (e.g. . size or charge). A common method for performing separation is to pass a fluid or fluid-like sample containing the desired target through a filter element having one or more pass- through apertures of a select dimension. By appropriate selection of the filter, one may preferentially obtain targeted objects on the filter while the residual fluid and other residues pass through the filter and are removed.
[ 0000 2] An apparatus may be employed that faci litates the inputting of the fluid sample, the passing of the sample through the filter, and the removal of the residual fluid. The design of the filter element and the collection apparatus may provide for the safe removal of the filtered material from the filter, and its subsequent mounting on a substrate suitable for further necessary processing and microscopic examination. Advantageously, the filter element and collection apparatus should not introduce any artifacts into the collected sample. Ideally, the distribution of the collected targeted cells should be uniform across the surface area of the filter.
BRIEF SUMMARY OF EMBODIMENTS
[ 0000 3] In embodiments, a membrane fi lter and membrane filter apparatus is disclosed for facilitating the preparation of a microscope slide containing a filtered sample. The filter material having defined tillering characteristics (such as porosity, charge, lipophilicity, etc. depending on the substrate desired to be preferentially filtered out) is mounted in a transport frame. After filtration, the loaded filter material is removed from the frame and adhesively bonded to a microscope slide.
[ 0000 4] In one embodiment, the frame comprises upper and lower frame portions, that are configured to articulate with one another so as to connect to one another at least one portion of the same. In one embodiment, the upper and lower frame portions are configured to press-fit with one another. In another embodiment, at least the upper or lower frame portion have an adhesive area associated therewith that allows for fixed articulation between both frame portions. The frame may be of any shape, including rectangular, and may comprise multiple portions and typically defines a void between frame portions.
|00051 Such frame embodiments are designed to house there-between a membrane that my span between portions of the frame. Such membrane may comprise delimiters that mark a selected area for placement in the void between frame portions. Such membrane may further comprise a data recordation area which may be interrogated for information pertaining to the attributes of the slide and/or sample, and/or interrogated for storage of information onto the data recordation area. The membrane may further comprise a filtration demarcation area designed to instruct a user to where sample should be placed for filtration (and where the fi ltered sample exists after application of filtration). The filtration demarcation area may be fabricated identical to the rest of the membrane, or may have special fi ltration characteristics unique to the membrane.
[0006] In an embodiment, the membrane having the filtration demarcation area and the data recordation area is placed between the upper and lower frame portions so as to course between frame elements. The framed delimited membrane may be applied to a suction apparatus to allow for quicker filtration through the membrane. Such suction apparatus may comprise a vessel with a vacuum conduit running there through and an upper sealed area surrounding a void of approximately the geometry of the filtration demarcation area of the membrane. Advantageously, the frame and upper portion of the suction apparatus are configured to allow the framed membrane to lie on top of the vessel such that the filtration demarcation area is positioned over a portion of the vacuum conduit.
|0007| The framed membrane may be removed from the frame after filtration, and the membrane affixed to a more solid substrate for review under a microscope. Of course, the membrane itself may be selected to be sufficient turgidity such that the membrane itsel f may be used as the slide itself. The microscope slide may comprise any substrate, but often preferentially glass or clear polymer. The microscope slide may be manufactured to have portions thereof that have activatable adhesive thereon such that the membrane adheres to the slide when applied. In an advantageous embodiment the slide has adhesive portions that are remote from the portion over which the filtration demarcation area of the membrane will reside. DESCRIPTION OF FIGURES
[ 0000 8] Certain embodiments can be best understood with the aid of the following figures:
Fig. 1 is a perspective drawing of the filter transport frame. A double sided adhesive is applied to the molded filter membrane tension/transportation frame.
Fig. 2 portrays the mounting of the filter membrane. Using an appropriate alignment fixture, the molded filter membrane tension'transportation frame is applied to the preprinted (barcode and deposition area) filter membrane. The membrane may be cut from a continuous roll.
Fig. 3 displays the completed filter element. The complete assembly comprises a molded filter transportation frame applied to the preprinted filter membrane.
Fig. 4 illustrates the mounting of the fi lter element in a suitable collection apparatus.
Fig. 5 illustrates the removal of the membrane after filtration and placement into an alignment fixture for transfer of the membrane to the sl ide. The membrane is then trimmed from the frame, just inside the frame edges, using a heated knife edge.
DETAILED DESCRIPTION
[ 0000 9] In embodiments, there is presented a membrane filter for separating and collecting structures contained in a fluid sample, such as cells, on the basis of their size. The design of membrane filter facilitates its safe insertion and removal from the collection apparatus and minimizes the risk of introduction of artifacts into the collected sample.
[ 0001 0] The advantages of the embodiments include 1 ) the elimination of the need for manual membrane handling thereby insuring sample integrity; and 2) the flatness of the filter element and the subsequent slide is suitable for automated microscope examination. In biological applications, ( 1 ) the cell distribution on the membrane surface is optimized for imaging; and (2) the filter is suitable for IF and FISH processing and staining protocols and is compatible with auto-staining systems;
[0001 1 ] In an embodiment, the fi lter material is manufactured as a strip or ribbon of suitable material such as TEM i.e., track etched membrane. The strip may comprise fi lter sections having porosity properties that permit the penetration of fluids and structures having less than a specified dimension. Structures larger than the specified dimension wi l l be captured by the filter material. In an embodiment, non-filtering sections may be placed adjacent to the filtering sections. The non-filtering sections may provide an area for inclusion of data media such as textual or bar-code identification markings, or other magnetically, optically, or electronically readable and/or writable media.
[00012] A single use filter element is manufactured by adhesively mounting a section of the filter material into a transportation frame. Other non-adhesive mounting methods, such as snap fit. may alternatively be employed. The transportation frame may be rectangular and substantially planar or, alternatively, it may be non-rectangular and substantially planar. The filter material may be stretched to insure that flatness is maintained when mounted in the rigid transportation frame. The transport frame may be designed for one use. or mav allow for new membrane to be added to the frame so as to course across the framed void . In one embodiment, the frame has a void in each of two parallel sections allowing new membrane to be added bv removing the filtered membrane from the frame through one void, and adding new membrane through the other void.
[00013] The transportation frame, containing the filter element, may be designed to be loaded into a collection apparatus. The collection apparatus may comprise an input reservoir, a filter element holder, and a discharge receptacle. The apparatus may be configured so that sample fluid loaded into a an input reservoir, containing the targeted cells, is made to pass through the filter material, and then to a discharge receptacle. The process may be effectuated by applying a positive pressure to the input reservoir and/or a negative pressure to the discharge receptacle. [00014] Following filtration, the transportation frame, containing the filter element charged with the collected cells, is removed from the collection apparatus. The transportation frame may be brought into planar contact with an adhesively coated microscope slide using a guiding template. The filter element adheres to the surface of the microscope slide. The filter element material may be cut to release it from the transport frame.
|00015] Now turning to Fig. 1. there is shown a frame that may find use in the present disclosure. Frame 10, as shown, comprises two parts an upper frame portion 20 and a lower frame portion 30. Upper frame portion 20 is shown to have adhesive portions thereon 20 which may be used to affix upper frame portion 20 to lower frame portion 30. The frame defines a void 25.
[00016] As shown in Fig. 2, membrane 10 is designed to have a delimited area set forth by delimiters 70 such that the delimited membrane may fit within void 25 of frame 10 of Fig. /. Membrane 10, as illustrated, comprises a filtration demarcation area 60 and a data recordation area 50.
[00017] Turning now to Fig. 5, there is shown a adjoined frame with a fixed membrane 80 there between, coursing through void 25 between parallel legs of the frame. As shown in Fig. 4 A, such framed membrane 80 can then be applied to a suction support apparatus, such as shown in Fig. 4A as 120. Apparatus 120 as shown comprise suction support structure 1 10 having a top portion 125 and bottom portion 130. Top portion has gasket 1 15 which defines a void area through which vacuum conduit 90 terminates. Vacuum conduit 90 terminates on other end in vacuum port 120 to which a vacuum may be affixed to allow for the formation of a vacuum throughout v acuum conduit 90. Framed membrane 80 is placed such that vacuum conduit 90 is positioned beneath filtration demarcation area 60. Fig 48 a top view of the framed membrane 80 sitting on the top gasketed portion of suction support structure 1 10.
[00018] As shown in Fig. 5, framed membrane 80 may be excised from frame 20 and then placed on a substrate, such as microscope slide 120. As shown, microscope slide 1 20 has adhesive portions 130 which allow for fixation of membrane 40 thereon with portion 120. which lacks such adhesive, providing a corresponding area against which filtration demarcation area 60 of membrane 40 may rest. STATEMENT REGARDING PREFERRED EMBODIMENTS
[ 0001 9] While the invention has been described with respect to preferred embodiments, those skilled in the art will readily appreciate that various changes and/or modifications can be made to the invention without departing from the spirit or scope of the invention as defined by the appended claims. All documents cited herein arc incorporated
Figure imgf000007_0001
reference herein where appropriate for teachings of additional or alternative detai ls, features and/or technical background.

Claims

What is claimed is:
1. A filter apparatus comprising: a substantially planar frame defining a void: a filter material, in the form of a sheet, fastened to said substantially planar frame coursing through said void; wherein said filter material comprises a data recordation area and a filtration demarcation area.
2. A filter, in accordance with claim 1 . wherein said filter material permits the passage of structures having a size less than a predefined value.
3. A filter, in accordance with claim 1 , wherein said filter material is fastened to said substantially planar frame by adhesive material .
4. A method for manufacture of a filter comprising: obtain a substantially planar frame defining a rectangular void: obtain filter material in the form of a sheet; co-planarly fasten said filter material to said substantially planar frame.
PCT/US2010/054138 2009-10-26 2010-10-26 Membrane filter system and apparatus WO2011056598A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US25493309P 2009-10-26 2009-10-26
US61/254,933 2009-10-26

Publications (2)

Publication Number Publication Date
WO2011056598A2 true WO2011056598A2 (en) 2011-05-12
WO2011056598A3 WO2011056598A3 (en) 2011-10-06

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107202803A (en) * 2017-04-24 2017-09-26 天津大学 A kind of objective table being imaged for computer demixing scan

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5252293A (en) * 1989-01-17 1993-10-12 Vladimir Drbal Analytical slide with porous filter membrane
JPH0985067A (en) * 1995-09-27 1997-03-31 Sapporo Breweries Ltd Membrane filter made of synthetic resin
JPH1066847A (en) * 1996-08-29 1998-03-10 Teijin Ltd Porous filter membrane and membrane module using the same
EP1037034A1 (en) * 1999-03-05 2000-09-20 The Automation Partnership (Cambridge) Limited Membrane filter assembly

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5252293A (en) * 1989-01-17 1993-10-12 Vladimir Drbal Analytical slide with porous filter membrane
JPH0985067A (en) * 1995-09-27 1997-03-31 Sapporo Breweries Ltd Membrane filter made of synthetic resin
JPH1066847A (en) * 1996-08-29 1998-03-10 Teijin Ltd Porous filter membrane and membrane module using the same
EP1037034A1 (en) * 1999-03-05 2000-09-20 The Automation Partnership (Cambridge) Limited Membrane filter assembly

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107202803A (en) * 2017-04-24 2017-09-26 天津大学 A kind of objective table being imaged for computer demixing scan

Also Published As

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