WO2011104695A3 - Detection of kras mutation in exon 2 by allele specific real time quantitative pcr (as-qpcr) - Google Patents

Detection of kras mutation in exon 2 by allele specific real time quantitative pcr (as-qpcr) Download PDF

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Publication number
WO2011104695A3
WO2011104695A3 PCT/IB2011/050819 IB2011050819W WO2011104695A3 WO 2011104695 A3 WO2011104695 A3 WO 2011104695A3 IB 2011050819 W IB2011050819 W IB 2011050819W WO 2011104695 A3 WO2011104695 A3 WO 2011104695A3
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WO
WIPO (PCT)
Prior art keywords
allele
qpcr
exon
detection
real time
Prior art date
Application number
PCT/IB2011/050819
Other languages
French (fr)
Other versions
WO2011104695A2 (en
Inventor
Luca Morandi
Original Assignee
GAMMAGENETICS Sàrl
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by GAMMAGENETICS Sàrl filed Critical GAMMAGENETICS Sàrl
Publication of WO2011104695A2 publication Critical patent/WO2011104695A2/en
Publication of WO2011104695A3 publication Critical patent/WO2011104695A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/106—Pharmacogenomics, i.e. genetic variability in individual responses to drugs and drug metabolism

Abstract

A method for conducting an allele specific polymerase chain reaction which comprises subjecting DNA which may contain either or both of first and second alleles to a polymerase chain reaction utilizing a primer pair in which one of the primers is complementary to said first allele, but which primer forms a mismatch with said second allele at the 3'-terminal nucleotide of the primer, and utilizing a DNA polymerase wherein said first allele is specifically amplified but no amplification of said second allele occurs.
PCT/IB2011/050819 2010-02-26 2011-02-25 Detection of kras mutation in exon 2 by allele specific real time quantitative pcr (as-qpcr) WO2011104695A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IB2010050843 2010-02-26
IBPCT/IB2010/050843 2010-02-26

Publications (2)

Publication Number Publication Date
WO2011104695A2 WO2011104695A2 (en) 2011-09-01
WO2011104695A3 true WO2011104695A3 (en) 2012-03-01

Family

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Family Applications (1)

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PCT/IB2011/050819 WO2011104695A2 (en) 2010-02-26 2011-02-25 Detection of kras mutation in exon 2 by allele specific real time quantitative pcr (as-qpcr)

Country Status (1)

Country Link
WO (1) WO2011104695A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102367478B (en) * 2011-10-25 2013-07-31 浙江大学 ARMS-qPCR (Allele Refractory Mutation System-quantitative Polymerase Chain Reaction) detection kit for KRAS (Kirsten Rat Sarcoma Viral Oncogene Homolog) gene mutation subtype and detection method
CN103930571A (en) * 2011-11-17 2014-07-16 瑞尼克斯有限公司 System and methods for selective molecular analysis
CN102796816B (en) * 2012-07-06 2014-04-16 陕西北美基因股份有限公司 Method for performing in-vitro accurate test on KRAS gene mutation
WO2014052613A2 (en) * 2012-09-26 2014-04-03 Insight Genetics, Inc. Methods and compositions relating to next generation sequencing for genetic testing in alk related cancers
CN104805207A (en) * 2015-04-29 2015-07-29 苏州工业园区为真生物医药科技有限公司 Kit for detecting kras gene mutation, and detection method of kit
JP6754202B2 (en) * 2016-03-15 2020-09-09 アークレイ株式会社 K-ras gene amplification forward primer set, K-ras gene amplification kit, K-ras gene amplification method, polymorphism analysis method, and drug efficacy determination method

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5639611A (en) * 1988-12-12 1997-06-17 City Of Hope Allele specific polymerase chain reaction
WO2000056916A2 (en) * 1999-03-18 2000-09-28 Exiqon A/S Detection of mutations in genes by specific lna primers
EP1247815A2 (en) * 2001-03-25 2002-10-09 Exiqon A/S Modified oligonucleotides and uses thereof
WO2003072814A2 (en) * 2002-02-26 2003-09-04 Roche Diagnostics Gmbh Improved method for allele-specific pcr
WO2008070370A2 (en) * 2006-11-02 2008-06-12 University Of Utah Research Foundation Oligonucleotides for use in allele-specific pcr

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5639611A (en) * 1988-12-12 1997-06-17 City Of Hope Allele specific polymerase chain reaction
WO2000056916A2 (en) * 1999-03-18 2000-09-28 Exiqon A/S Detection of mutations in genes by specific lna primers
EP1247815A2 (en) * 2001-03-25 2002-10-09 Exiqon A/S Modified oligonucleotides and uses thereof
WO2003072814A2 (en) * 2002-02-26 2003-09-04 Roche Diagnostics Gmbh Improved method for allele-specific pcr
WO2008070370A2 (en) * 2006-11-02 2008-06-12 University Of Utah Research Foundation Oligonucleotides for use in allele-specific pcr

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
JOHNSON M P ET AL: "LOCKED NUCLEIC ACID (LNA) SINGLE NUCLEOTIDE POLYMORPHISM (SNP) GENOTYPE ANALYSIS AND VALIDATION USING REAL-TIME PCR", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 32, no. 6, 26 March 2004 (2004-03-26), pages E55 - 01, XP009069933, ISSN: 0305-1048 *
LATORRA D ET AL: "ENHANCED ALLELE-SPECIFIC PCR DISCRIMINATION IN SNP GENOTYPING USING 3' LOCKED NUCLEIC ACID (LNA) PRIMERS", HUMAN MUTATION, JOHN WILEY & SONS, INC, US, vol. 22, no. 1, 1 July 2003 (2003-07-01), pages 79 - 85, XP009043978, ISSN: 1059-7794, DOI: 10.1002/HUMU.10228 *
STRAND ET AL: "Real-time detection and quantification of mitochondrial mutations with oligonucleotide primers containing locked nucleic acid", CLINICA CHIMICA ACTA, ELSEVIER BV, AMSTERDAM, NL, vol. 390, no. 1-2, 16 January 2008 (2008-01-16), pages 126 - 133, XP022499213, ISSN: 0009-8981, DOI: 10.1016/J.CCA.2008.01.010 *

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Publication number Publication date
WO2011104695A2 (en) 2011-09-01

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