WO2011154961A1 - Advance method of preparation of bacterial formulation using potash mobilizing bacteria that mobilize potash and make it available to crop plant - Google Patents

Advance method of preparation of bacterial formulation using potash mobilizing bacteria that mobilize potash and make it available to crop plant Download PDF

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Publication number
WO2011154961A1
WO2011154961A1 PCT/IN2010/000389 IN2010000389W WO2011154961A1 WO 2011154961 A1 WO2011154961 A1 WO 2011154961A1 IN 2010000389 W IN2010000389 W IN 2010000389W WO 2011154961 A1 WO2011154961 A1 WO 2011154961A1
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Prior art keywords
potash
mobilizing
pac
culture
product according
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PCT/IN2010/000389
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French (fr)
Inventor
Chetan S. Patel
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Patel, Babubhai C.
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Priority to PCT/IN2010/000389 priority Critical patent/WO2011154961A1/en
Publication of WO2011154961A1 publication Critical patent/WO2011154961A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00—Other organic fertilisers
    • C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like

Definitions

  • the present invention relates generally to a material and advanced method of making a bacterial based product that fix atmospheric nitrogen from air and makes available to plant.
  • the invention relates to the method for increasing the rate of conversion of atmospheric nitrogen into ammonia in a microorganism of the genus Rhizobium, by increasing the intracellular level of an activator protein which is capable of activating the transcription of DNA of the microorganism encoding one or more proteins capable of effecting such conversion in the microorganism.
  • the invention relates to the formulation of an organic agricultural fertilizer.
  • This product meets the standards of the U.S. Department of Agriculture (USD A).
  • USD A U.S. Department of Agriculture
  • the material used is municipal sludge from treated municipal plants.
  • the raw material meets the guidelines of the United States Environmental Protection Agency (USEPA).
  • USEPA United States Environmental Protection Agency
  • the sludge is treated with various additives to eliminate pathogens.
  • Globally acceptable additives are added to the base material to produce the fertilizer.
  • the macronutrient materials include phosphates, potash, and nitrates.
  • liquid ammonia is used to add nitrogen and eliminate pathogens..
  • the invention relates to Fertilizer compositions comprising: A) an effective quantity of an inorganic or organic fertilizer; and B) a quantity of beneficial microorganisms sufficient to further enhance plant growth when the fertilizer composition is applied to soil and/or ⁇ control pathogens in the soil, and methods for their use.
  • the invention relates to to an improved fertilizing composition of the type containing nitrogen, phosphorus and potassium, with the improvement being the addition of 1-methyl- 4-(l-methylethenyl)cyclohexene (i.e., limonene) as an insecticide and fungicide.
  • 1-methyl- 4-(l-methylethenyl)cyclohexene i.e., limonene
  • Xylitol or D-xylulose is produced through direct fermentation frqm glucose by culturing a microorganism belonging to the genus Gluconobacter, Acetobacter or Frateuria, and having an ability to produce xylitol or D-xylulose in a suitable medium to accumulate xylitol or D-xylulose in the medium, and collecting xylitol or D-xylulose from the medium.
  • the invention provides novel methods for improving plant quality and yield in the presence of pathogens.
  • the method increases the levels of pathogenesis-related proteins, such as PR1 , phenylalanine ammonia lyase, or plant cell wall proteins such as hydroxyproline-rich glycoproteins, in a plant by contacting the plant with a plant systemic inducer and a reactive oxygen species wherein the amount of the reactive oxygen species is sufficient to increase the amount of the pathogenesis-related protein above the level induced by the plant systemic inducer in the absence of the reactive oxygen species.
  • a preferred reactive oxygen species is peracetic acid; a preferred plant systemic inducer is salicylic acid.
  • Potassium fulfills numerous vital functions in various processes in plants, animals and humans. For adequate nutrient supply of potassium, soil reserves are essentially required, which commonly contain more potassium than any other nutrient, including nitrogen.
  • K potassium
  • the rich sources of this nutrient in human diet are milk, fruit * juice, root vegetables and bananas.
  • Potash has three main uses: fertilizer, livestock feed supplements and industrial processes. 95% of world's potash is used in fertilizers, while the rest is used for feed supplements and industrial production.
  • Potash is a key ingredient in fertilizers that enhances water retention of plants, increases crop yields and plants' disease resistance.
  • the present invention has been made with the foregoing background in mind and its object resides in providing a material and method of rationally producing a high quality of potash mobilizing bio-fertilizer at the highest economical level using raw material which can be easily procured at the most inexpensive cost.
  • the inventor has invented a composition and method for improving crop yields, involving potash mobilizing bacteria from pure culture of bacteria i.e. Frateuria aurentia , which mobilizes the immobilized potash and produces plant growth promoting substances which benefit the plant in a multifaceted way.
  • the subject invention concerns materials and methods of preparing potash mobilizing bacteria from pure culture of bacteria i.e. Frateuria aurentia. This is rich pota'sh immobilizers. This self-evolving formulation will bring you a new consortium every time.
  • Step-1 Nucleus culture (Maintain culture) Pure culture of bacteria ⁇ Frateuria aurentia) is inoculated aseptically on plate having 20 ml PAC-010 Agar media; such two to three plates are generally inoculated. Such inoculated plate is maintained in BOD incubator at 27 ⁇ 1 °C for 6 to 7 days with 12/12 hr lighting cycle.
  • Composition for one liter of PAC-010 agar media is 5 gm Dextrose, 1 gm MgSO 4 7H 2 O, 5 gm Yeast extract, 5 gm Peptone, 20 gm Agar agar and 1000 ml distilled water)
  • Step-2 Starter culture (In 100 ml flask)
  • Culture grown on plate is inoculated aseptically in a flask (250 ml capacity) having 100 ml PAC-010 broth media with the help of inoculating loop, and allow to grow at 27 ⁇ 1 °C for at least 3 to 4 days.
  • flask is put on shaker with agitation of media at 150 RPM.
  • Composition for one liter of PAC-010 agar media is 5 gm Dextrose, 1 gm MgSO 4 7H 2 O, 5 gm Yeast extract, 5 gm Peptone and 1000 ml distilled water
  • Step-3 Growth culture (In 1000 ml flasks).
  • step-2 50 ml culture from this grown culture is inoculated aseptically in 500 ml PAC-010 broth media in a 1000 ml capacity flask for further growth at 27 ⁇ 1 °C for at least 3 to 4 days on shaker with 150 RPM, such two flasks is prepared.
  • Composition for one liter of PAC-010 agar media is 5 gm Dextrose, 1 gm MgS0 4 7H 2 0, 5 gm Yeast extract, 5 gm Peptone and 1000 ml distilled water
  • Step-4 Seed Fermentor (In small fermentor of 10 lit capacities)
  • One (1) lit grown culture in step-3 is inoculated aseptically in small fermenter having 10 litter PAC-010 broth media, and allow for further growth at 27 ⁇ 1 °C for at least 3 to 4 days with agitation by motor at 100 RPM.
  • Composition for one liter of PAC-010 agar media is 5 gm Dextrose, 1 gm MgSO 4 7H 2 O, 5 gm Yeast extract, 5 gm Peptone and .1000 ml distilled water
  • Step-5 Production Fermentor (In Big fermentor of 200 lit capacities)
  • Ten (10) lit culture grown in step-4 is inoculated aseptically in Big fermenter having 200 litter PAC-010 media (Composition for one liter of PAC-010 agar media is 5 gm Dextrose, 1 gm MgSO 4 7H 2 O, 5 gm Yeast extract, 5 gm Peptone and 1000 ml distilled water), and allowed for further growth at 27 ⁇ 1 °C for at least 3 to 4 days with agitation by motor at 100 RPM Step-6 Bacterial Separation
  • Final output from big fermentor is centrifuge by using advance centrifugation technology. By this separated bacteria are use for formulation of product whereas remaining liquid is throughout.
  • Recovered bacteria form centrifuge is mix in a set of ration with suitable carrier composed of zero TDS water etc and then homogenized it for equivalently of formulation.
  • Material prepared as above is then packed in 100 ml, 250 ml and 500 ml packing by using bottle or pouch packing machine.
  • Step-09 Distrubution to farmer, retailer, distributor etc
  • the final material prepared has to be used in following form:

Abstract

The subject invention concerns materials and methods of preparing improved potash mobilizing bacterial based product containing Frateuria aurentia producing plant growth promoting substances which benefit the plant in a multifaceted way. During their growth, they mobilize potash and make it available to crops. It also increases efficiency of chemical fertilizer.

Description

TITLE- Advance Method Of Preparation Of Bacterial Formulation Using Potash Mobilizing Bacteria That Mobilize Potash And Make It Available To Crop Plant.
PREAMBLE OF INVENTION- This invention in particular describes the nature of the invention and the manner in which it is to be performed.
FIELD OF INVENTION- The present invention relates generally to a material and advanced method of making a bacterial based product that fix atmospheric nitrogen from air and makes available to plant.
PRIOR ART
In the existing system as given in WIPO Patent Application No. PCT/BR2002/000013, wherein, the invention relates to the method for increasing the rate of conversion of atmospheric nitrogen into ammonia in a microorganism of the genus Rhizobium, by increasing the intracellular level of an activator protein which is capable of activating the transcription of DNA of the microorganism encoding one or more proteins capable of effecting such conversion in the microorganism.
[i] In the existing system as given in European Patent Application No. EP0341736, wherein, the invention relates to a fertilizer of bacterial biomass and potassium magnesium fertilizer for fertilizing alpine pasture and ski slopes, and revitalising forest plantations..
In the existing system as given in United States Patent Application No. 20050056064, wherein, .the invention relates to the formulation of an organic agricultural fertilizer. This product meets the standards of the U.S. Department of Agriculture (USD A). The material used is municipal sludge from treated municipal plants. The raw material meets the guidelines of the United States Environmental Protection Agency (USEPA). The sludge is treated with various additives to eliminate pathogens. Globally acceptable additives are added to the base material to produce the fertilizer. The macronutrient materials include phosphates, potash, and nitrates. In addition, liquid ammonia is used to add nitrogen and eliminate pathogens..
In the existing system as given in United States Patent Application No. 6228806, wherein, the invention relates to Fertilizer compositions comprising: A) an effective quantity of an inorganic or organic fertilizer; and B) a quantity of beneficial microorganisms sufficient to further enhance plant growth when the fertilizer composition is applied to soil and/or · control pathogens in the soil, and methods for their use..
In the existing system as given in United States Patent Application No. 5728648, wherein, the invention relates to to an improved fertilizing composition of the type containing nitrogen, phosphorus and potassium, with the improvement being the addition of 1-methyl- 4-(l-methylethenyl)cyclohexene (i.e., limonene) as an insecticide and fungicide.
In .the existing system as given in United States Patent Application No. 6221634 , wherein the invention relates to Xylitol or D-xylulose is produced through direct fermentation frqm glucose by culturing a microorganism belonging to the genus Gluconobacter, Acetobacter or Frateuria, and having an ability to produce xylitol or D-xylulose in a suitable medium to accumulate xylitol or D-xylulose in the medium, and collecting xylitol or D-xylulose from the medium.
In the existing system as given in United States Patent Application No. 7691630, wherein, the invention provides novel methods for improving plant quality and yield in the presence of pathogens. The method increases the levels of pathogenesis-related proteins, such as PR1 , phenylalanine ammonia lyase, or plant cell wall proteins such as hydroxyproline-rich glycoproteins, in a plant by contacting the plant with a plant systemic inducer and a reactive oxygen species wherein the amount of the reactive oxygen species is sufficient to increase the amount of the pathogenesis-related protein above the level induced by the plant systemic inducer in the absence of the reactive oxygen species. A preferred reactive oxygen species is peracetic acid; a preferred plant systemic inducer is salicylic acid.
PARENT CASE DATE:
CROSS-REFERENCE TO RELATED APPLICATIONS
This application is a divisional of application number 339/MUM/2010, filed February, 02, 2010, now pending.
BACKGROUND OF THE INVENTION
Potassium fulfills numerous vital functions in various processes in plants, animals and humans. For adequate nutrient supply of potassium, soil reserves are essentially required, which commonly contain more potassium than any other nutrient, including nitrogen.
For an adult human being, approximately 2 grams of potassium (K) is required per day, even though a typical person will take in 2.8-4.5 grams/day. There is no health risks associated with potassium. The rich sources of this nutrient in human diet are milk, fruit * juice, root vegetables and bananas.
Nitrogen; phosphorus, and potassium are three of the most essential nutrients that a plant needs to grow. Potash plays an important role in helping plants to absorb potassium required to thrive.
Potash has three main uses: fertilizer, livestock feed supplements and industrial processes. 95% of world's potash is used in fertilizers, while the rest is used for feed supplements and industrial production.
Potash is a key ingredient in fertilizers that enhances water retention of plants, increases crop yields and plants' disease resistance.
As is well known by any expert in the art, there have been hitherto made a number of proposals as to a method of producing organic fertilizer. To practice the conventional methods various kinds of raw materials and processes were employed but each of them has drawbacks in terms of procurement of raw material and cost. OBJECT OF THE INVENTION
Hence, the present invention has been made with the foregoing background in mind and its object resides in providing a material and method of rationally producing a high quality of potash mobilizing bio-fertilizer at the highest economical level using raw material which can be easily procured at the most inexpensive cost.
STATEMENT OF INVENTION-
The inventor has invented a composition and method for improving crop yields, involving potash mobilizing bacteria from pure culture of bacteria i.e. Frateuria aurentia , which mobilizes the immobilized potash and produces plant growth promoting substances which benefit the plant in a multifaceted way.
DETAILED DESCRIPTION OF INVENTION-
The subject invention concerns materials and methods of preparing potash mobilizing bacteria from pure culture of bacteria i.e. Frateuria aurentia. This is rich pota'sh immobilizers. This self-evolving formulation will bring you a new consortium every time.
It mobilizes the immobilized potash and produces plant growth promoting substances which benefit the plant in a multifaceted way.
Material and Method used in preparing the above said innovative combination is as under -
Step-1 Nucleus culture (Maintain culture) Pure culture of bacteria {Frateuria aurentia) is inoculated aseptically on plate having 20 ml PAC-010 Agar media; such two to three plates are generally inoculated. Such inoculated plate is maintained in BOD incubator at 27 ± 1 °C for 6 to 7 days with 12/12 hr lighting cycle. (Composition for one liter of PAC-010 agar media is 5 gm Dextrose, 1 gm MgSO47H2O, 5 gm Yeast extract, 5 gm Peptone, 20 gm Agar agar and 1000 ml distilled water)
Step-2 Starter culture (In 100 ml flask)
Culture grown on plate is inoculated aseptically in a flask (250 ml capacity) having 100 ml PAC-010 broth media with the help of inoculating loop, and allow to grow at 27 ± 1 °C for at least 3 to 4 days. For superior growth, flask is put on shaker with agitation of media at 150 RPM. (Composition for one liter of PAC-010 agar media is 5 gm Dextrose, 1 gm MgSO47H2O, 5 gm Yeast extract, 5 gm Peptone and 1000 ml distilled water)
Step-3 Growth culture (In 1000 ml flasks
After sufficient growth in 250 ml flask (step-2), 50 ml culture from this grown culture is inoculated aseptically in 500 ml PAC-010 broth media in a 1000 ml capacity flask for further growth at 27 ± 1 °C for at least 3 to 4 days on shaker with 150 RPM, such two flasks is prepared. (Composition for one liter of PAC-010 agar media is 5 gm Dextrose, 1 gm MgS047H20, 5 gm Yeast extract, 5 gm Peptone and 1000 ml distilled water)
Step-4 Seed Fermentor (In small fermentor of 10 lit capacities)
One (1) lit grown culture in step-3 is inoculated aseptically in small fermenter having 10 litter PAC-010 broth media, and allow for further growth at 27 ± 1 °C for at least 3 to 4 days with agitation by motor at 100 RPM. (Composition for one liter of PAC-010 agar media is 5 gm Dextrose, 1 gm MgSO47H2O, 5 gm Yeast extract, 5 gm Peptone and .1000 ml distilled water)
Step-5 Production Fermentor (In Big fermentor of 200 lit capacities)
Ten (10) lit culture grown in step-4 is inoculated aseptically in Big fermenter having 200 litter PAC-010 media (Composition for one liter of PAC-010 agar media is 5 gm Dextrose, 1 gm MgSO47H2O, 5 gm Yeast extract, 5 gm Peptone and 1000 ml distilled water), and allowed for further growth at 27 ± 1 °C for at least 3 to 4 days with agitation by motor at 100 RPM Step-6 Bacterial Separation
Final output from big fermentor is centrifuge by using advance centrifugation technology. By this separated bacteria are use for formulation of product whereas remaining liquid is throughout.
Step-7 Formulation
Recovered bacteria form centrifuge is mix in a set of ration with suitable carrier composed of zero TDS water etc and then homogenized it for equivalently of formulation.
Step-8 Packging
Material prepared as above is then packed in 100 ml, 250 ml and 500 ml packing by using bottle or pouch packing machine.
Step-09 - Distrubution to farmer, retailer, distributor etc
The final material prepared has to be used in following form:
Dose in one Acre: In case of Seed treatment add 100 ml per seed required per hector
For soil application add 100 ml to 250 ml per hector

Claims

CLAIMS I claim,
1. A material and method of preparing an improved potash mobilizing bacterial based product containing Frateuria aurentia, thereby producing plant growth promoting substances which benefit the plant in a multifaceted way and increasing the efficiency of fertilizer, the method comprising steps of nucleus culture, starter culture, growth culture, seed fermentor, production fermentor, bacterial separation, formulation and packing the final output.
.
2. A material and method of preparing improved potash mobilizing bacterial product according to claim 1, by inoculating aseptically pure culture of bacteria namely Frateuria aurentia on two to three plate having 20 ml PAC-010 Agar media and maintained in BOD incubator at 27 ± 1 °C for 6 to 7 days with 12/12 hr lighting cycle, wherein composition for one liter of PAC-010 agar media contains 5 gm Dextrose, 1 gm MgSO47H2O, 5 gm Yeast extract, 5 gm Peptone, 20 gm Agar agar and 1000 ml distilled water.
3. A material and method of preparing improved potash mobilizing bacterial product according to claim 1, where in culture grown on plate is inoculated aseptically in a flask of 250 ml capacity having 100 ml PAC-010 broth media with the help Of inoculating loop, and allowing to grow at 27 ± 1 °C for at least 3 to 4 days, wherein for superior growth, flask is put on shaker with agitation of media at 150 RPM and wherein composition for one liter of PAC-010 broth media is 5 gm Dextrose, 1 gm MgS047H20, 5 gm Yeast extract, 5 gm Peptone and 1000 ml distilled water.
4. A material and method of preparing improved potash mobilizing bacterial product according to claim 1 , wherein after sufficient growth in 250 ml flask, 50 ml culture from this grown culture is inoculated aseptically in 500 ml PAC-010 broth media in a 1000 ml capacity flask for further growth at 27 ± 1 °C for at least 3 to 4 days on shaker with 150 RPM, such two flasks is prepared, wherein composition for one liter of PAC-010 broth media contain 5 gm Dextrose, 1 gm MgS047H20, 5 gm Yeast extract, 5 gm Peptone and 1000 ml distilled water.
5. A material and method of preparing improved potash mobilizing bacterial product according to claim 1, One (1) lit grown culture according to method cited in claim' 4 is inoculated aseptically in small fermenter having 10 litter PAC-010 broth media, and allow for further growth at 27 ± 1 °C for at least 3 to 4 days with agitation by motor at 100 RPM, wherein composition for one liter of PAC-010 broth media contains 5 gm Dextrose, 1 gm MgSO47H2O, 5 gm Yeast extract, 5 gm Peptone and 1000 ml distilled water.
6. A material and method of preparing improved potash mobilizing bacterial product according to claim 1 , wherein ten (10) lit culture grown is inoculated aseptically in Big fermenter having 200 litter PAC-010 media, wherein composition for for one liter of PAC-010 agar media is 5 gm Dextrose, 1 gm MgS047H20, 5 gm Yeast extract, 5 gm Peptone and 1000 ml distilled water, and allow for further growth at 27 ± 1 °C for at least 3 to 4 days with agitation by motor at 100 RPM.
7. A material and method of preparing improved potash mobilizing bacterial product according to claim 1, wherein the final output from big fermentor is centrifuge by using advance centrifugation technology and thereby this separated bacteria are use for formulation of product whereas remaining liquid is throughout.
8. A material and method of preparing improved potash mobilizing bacterial product according to claim 1, Recovered bacteria form centrifuge is mix in a set of ration with suitable carrier composed of zero TDS water etc and then homogenized it for equivalently of formulation.
9. A material and method of preparing improved potash mobilizing bacterial product according to claim 1, further comprising of packing the final output in 100 ml, 250 ml and 500 ml packing by using bottle or pouch packing machine for distribution to farmer, retailer, distributor, etc.
10. A material and method of preparing improved potash mobilizing bacterial product according to claim 1, wherein the final material prepared has to be used in following form dose in one Acre: In case of Seed treatment add 100 ml per seed required per hector; for soil application add 100 ml to 250 ml per hector.
PCT/IN2010/000389 2010-06-09 2010-06-09 Advance method of preparation of bacterial formulation using potash mobilizing bacteria that mobilize potash and make it available to crop plant WO2011154961A1 (en)

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