WO2012068392A3 - Low cost, portable sensor for molecular assays - Google Patents

Low cost, portable sensor for molecular assays Download PDF

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Publication number
WO2012068392A3
WO2012068392A3 PCT/US2011/061227 US2011061227W WO2012068392A3 WO 2012068392 A3 WO2012068392 A3 WO 2012068392A3 US 2011061227 W US2011061227 W US 2011061227W WO 2012068392 A3 WO2012068392 A3 WO 2012068392A3
Authority
WO
WIPO (PCT)
Prior art keywords
sensor
label
low cost
measurements
portable sensor
Prior art date
Application number
PCT/US2011/061227
Other languages
French (fr)
Other versions
WO2012068392A2 (en
Inventor
Alex H. Pai
Steven A. Chapman
Seyed Ali Hajimiri
Original Assignee
California Institute Of Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by California Institute Of Technology filed Critical California Institute Of Technology
Publication of WO2012068392A2 publication Critical patent/WO2012068392A2/en
Publication of WO2012068392A3 publication Critical patent/WO2012068392A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
    • G01N27/3278—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction involving nanosized elements, e.g. nanogaps or nanoparticles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/72—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating magnetic variables
    • G01N27/74—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating magnetic variables of fluids
    • G01N27/745—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating magnetic variables of fluids for detecting magnetic beads used in biochemical assays
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings

Abstract

An integrated sensor that is capable of discriminating the distance of a label from the sensor without using an optical signal. The label is attached to a single probe molecule or a group of probe molecules that interacts with a single or group of target molecules. As a consequence of this interaction, the probe molecule and/or the target molecule undergo a conformal change. This conformal change leads to perturbations in the distance of the label from the sensor. Thus, measurements and properties such as the concentration and the identity of one or more target molecules can be discerned from signals generated by the sensor (or by a plurality of sensors in a sensor array) and subjected to analysis using general purpose programmable computers programmed with suitable software that controls the analytical process, and such measurements and properties can be provided as a result of the analysis.
PCT/US2011/061227 2010-11-17 2011-11-17 Low cost, portable sensor for molecular assays WO2012068392A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US41460510P 2010-11-17 2010-11-17
US61/414,605 2010-11-17

Publications (2)

Publication Number Publication Date
WO2012068392A2 WO2012068392A2 (en) 2012-05-24
WO2012068392A3 true WO2012068392A3 (en) 2012-09-27

Family

ID=46084654

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2011/061227 WO2012068392A2 (en) 2010-11-17 2011-11-17 Low cost, portable sensor for molecular assays

Country Status (1)

Country Link
WO (1) WO2012068392A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20210204840A1 (en) * 2016-04-25 2021-07-08 Eccrine Systems, Inc. Eab biosensors for detecting sweat analytes

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030077649A1 (en) * 2001-10-23 2003-04-24 Cho Yoon-Kyoung Method and sensor for detecting the binding of biomolecules by shear stress measurement
US20070020641A1 (en) * 2003-03-25 2007-01-25 The Regents Of The University Of California Reagentless, reusable, bioelectronic detectors employing aptamers
US20090142847A1 (en) * 2005-06-17 2009-06-04 University Of Maryland Biotechnology Institute Metal enhanced fluorescence-based sensing methods
US20100267169A1 (en) * 2009-04-17 2010-10-21 California Institute Of Technology Electromagnetic molecular sensors and methods of using same

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030077649A1 (en) * 2001-10-23 2003-04-24 Cho Yoon-Kyoung Method and sensor for detecting the binding of biomolecules by shear stress measurement
US20070020641A1 (en) * 2003-03-25 2007-01-25 The Regents Of The University Of California Reagentless, reusable, bioelectronic detectors employing aptamers
US20090142847A1 (en) * 2005-06-17 2009-06-04 University Of Maryland Biotechnology Institute Metal enhanced fluorescence-based sensing methods
US20100267169A1 (en) * 2009-04-17 2010-10-21 California Institute Of Technology Electromagnetic molecular sensors and methods of using same

Also Published As

Publication number Publication date
WO2012068392A2 (en) 2012-05-24

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