WO2012088339A3 - Nanopore-based single dna molecule characterization using speed bumps - Google Patents

Nanopore-based single dna molecule characterization using speed bumps Download PDF

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Publication number
WO2012088339A3
WO2012088339A3 PCT/US2011/066627 US2011066627W WO2012088339A3 WO 2012088339 A3 WO2012088339 A3 WO 2012088339A3 US 2011066627 W US2011066627 W US 2011066627W WO 2012088339 A3 WO2012088339 A3 WO 2012088339A3
Authority
WO
WIPO (PCT)
Prior art keywords
nanopore
dna
test dna
unknown
dna molecule
Prior art date
Application number
PCT/US2011/066627
Other languages
French (fr)
Other versions
WO2012088339A2 (en
Inventor
Randy Davis
Roger Chen
Original Assignee
Genia Technologies, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genia Technologies, Inc. filed Critical Genia Technologies, Inc.
Publication of WO2012088339A2 publication Critical patent/WO2012088339A2/en
Publication of WO2012088339A3 publication Critical patent/WO2012088339A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y10/00—Nanotechnology for information processing, storage or transmission, e.g. quantum computing or single electron logic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S977/00—Nanotechnology
    • Y10S977/902—Specified use of nanostructure
    • Y10S977/904—Specified use of nanostructure for medical, immunological, body treatment, or diagnosis
    • Y10S977/924—Specified use of nanostructure for medical, immunological, body treatment, or diagnosis using nanostructure as support of dna analysis

Abstract

The present invention relates to a method of using nanopore to obtain sequence information of an unknown structure (unknown DNA) in a ss test DNA. The method comprises using speed bump to stall the ss test DNA in the nanopore at random positions of the ss test DNA to obtain sequence information of each and every nucleotides of the unknown DNA, and to construct the whole sequence of the unknown DNA. The present invention also relates to a novel method of trapping a ss test DNA in a nanopore using two bulky structures formed under different conditions (e.g. different temperature), and the bulky structures are able to keep the ss test DNA trapped in a nanopore at a working temperature.
PCT/US2011/066627 2010-12-22 2011-12-21 Nanopore-based single dna molecule characterization using speed bumps WO2012088339A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201061426323P 2010-12-22 2010-12-22
US201061426322P 2010-12-22 2010-12-22
US61/426,323 2010-12-22
US61/426,322 2010-12-22

Publications (2)

Publication Number Publication Date
WO2012088339A2 WO2012088339A2 (en) 2012-06-28
WO2012088339A3 true WO2012088339A3 (en) 2012-11-01

Family

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Family Applications (2)

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PCT/US2011/066632 WO2012088341A2 (en) 2010-12-22 2011-12-21 Nanopore-based single dna molecule characterization, identification and isolation using speed bumps
PCT/US2011/066627 WO2012088339A2 (en) 2010-12-22 2011-12-21 Nanopore-based single dna molecule characterization using speed bumps

Family Applications Before (1)

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PCT/US2011/066632 WO2012088341A2 (en) 2010-12-22 2011-12-21 Nanopore-based single dna molecule characterization, identification and isolation using speed bumps

Country Status (3)

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US (6) US8845880B2 (en)
GB (1) GB2500360B (en)
WO (2) WO2012088341A2 (en)

Families Citing this family (52)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007146158A1 (en) * 2006-06-07 2007-12-21 The Trustees Of Columbia University In The City Of New York Dna sequencing by nanopore using modified nucleotides
JP2012529056A (en) * 2009-06-05 2012-11-15 アリゾナ・ボード・オブ・リージェンツ・アクティング・フォー・アンド・オン・ビハーフ・オブ・アリゾナ・ステイト・ユニバーシティ Integrated photoelectrochemical sensor for nitric oxide in gaseous samples
US8324914B2 (en) 2010-02-08 2012-12-04 Genia Technologies, Inc. Systems and methods for characterizing a molecule
US9678055B2 (en) 2010-02-08 2017-06-13 Genia Technologies, Inc. Methods for forming a nanopore in a lipid bilayer
US9605307B2 (en) 2010-02-08 2017-03-28 Genia Technologies, Inc. Systems and methods for forming a nanopore in a lipid bilayer
CN107083421A (en) 2010-12-17 2017-08-22 纽约哥伦比亚大学理事会 The DNA detected using the nucleotides through modification and nano-pore is sequenced in synthesis
WO2012088341A2 (en) * 2010-12-22 2012-06-28 Genia Technologies, Inc. Nanopore-based single dna molecule characterization, identification and isolation using speed bumps
US8962242B2 (en) 2011-01-24 2015-02-24 Genia Technologies, Inc. System for detecting electrical properties of a molecular complex
US9110478B2 (en) 2011-01-27 2015-08-18 Genia Technologies, Inc. Temperature regulation of measurement arrays
WO2013067208A1 (en) 2011-11-02 2013-05-10 Genia Technologies, Inc. Systems and methods for determining genetic data
US20130244340A1 (en) * 2012-01-20 2013-09-19 Genia Technologies, Inc. Nanopore Based Molecular Detection and Sequencing
EP2815235B1 (en) 2012-02-15 2020-04-15 Oxford Nanopore Technologies Limited Aptamer method
US9850534B2 (en) 2012-02-16 2017-12-26 Genia Technologies, Inc. Methods for creating bilayers for use with nanopore sensors
US8986629B2 (en) 2012-02-27 2015-03-24 Genia Technologies, Inc. Sensor circuit for controlling, detecting, and measuring a molecular complex
ES2906186T3 (en) 2012-04-09 2022-04-13 Univ Columbia Method for the preparation of nanopore and uses thereof
CA2876095A1 (en) 2012-06-15 2013-12-19 Genia Technologies, Inc. Chip set-up and high-accuracy nucleic acid sequencing
EP3674412A1 (en) 2012-06-20 2020-07-01 The Trustees of Columbia University in the City of New York Nucleic acid sequencing by nanopore detection of tag molecules
WO2014041337A1 (en) * 2012-09-14 2014-03-20 Oxford Nanopore Technologies Limited Sample preparation method
US9995728B2 (en) 2012-11-06 2018-06-12 Oxford Nanopore Technologies Ltd. Quadruplex method
US9605309B2 (en) 2012-11-09 2017-03-28 Genia Technologies, Inc. Nucleic acid sequencing using tags
US9759711B2 (en) 2013-02-05 2017-09-12 Genia Technologies, Inc. Nanopore arrays
GB201318465D0 (en) 2013-10-18 2013-12-04 Oxford Nanopore Tech Ltd Method
WO2014144898A1 (en) 2013-03-15 2014-09-18 The Trustees Of Columbia University In The City Of New York Method for detecting multiple predetermined compounds in a sample
CN105992634B (en) 2013-08-30 2019-06-14 华盛顿大学商业中心 Selective modification polymer subunits are to improve the analysis based on nano-pore
US9551697B2 (en) 2013-10-17 2017-01-24 Genia Technologies, Inc. Non-faradaic, capacitively coupled measurement in a nanopore cell array
US9322062B2 (en) 2013-10-23 2016-04-26 Genia Technologies, Inc. Process for biosensor well formation
CA2926138A1 (en) 2013-10-23 2015-04-30 Genia Technologies, Inc. High speed molecular sensing with nanopores
US10047392B2 (en) 2014-02-21 2018-08-14 Northeastern University Fluorescence-based analysis of biopolymers using nanopores
MA39774A (en) 2014-03-24 2021-05-12 Roche Sequencing Solutions Inc CHEMICAL PROCESSES TO PRODUCE LABEL NUCLEOTIDES
EP3143158A1 (en) * 2014-05-14 2017-03-22 Stratos Genomics, Inc. Translocation control for sensing by a nanopore
US10519499B2 (en) * 2014-07-31 2019-12-31 Illumina, Inc. Hybrid nanopore sensors
JP6824881B2 (en) 2014-10-17 2021-02-03 オックスフォード ナノポール テクノロジーズ リミテッド Methods for characterizing nanopore RNA
WO2016106690A1 (en) * 2014-12-31 2016-07-07 Coyote Bioscience Co., Ltd. Detection of nucleic acid molecules using nanopores and complexing moieties
RU2017127990A (en) 2015-02-02 2019-03-04 Ту Пор Гайс, Инк. DETECTION BY MEANS OF NANOPORA OF TARGET POLINUCLEOTIDES FROM THE SAMPLE BACKGROUND
GB201508003D0 (en) * 2015-05-11 2015-06-24 Oxford Nanopore Tech Ltd Apparatus and methods for measuring an electrical current
WO2017024017A1 (en) 2015-08-06 2017-02-09 Pacific Biosciences Of California, Inc. Systems and methods for selectively addressing sparsely arranged electronic measurement devices
CN108474760A (en) * 2015-11-23 2018-08-31 双孔人公司 Target modification for tracking and detecting
US10859562B2 (en) 2016-02-29 2020-12-08 Iridia, Inc. Methods, compositions, and devices for information storage
US10640822B2 (en) 2016-02-29 2020-05-05 Iridia, Inc. Systems and methods for writing, reading, and controlling data stored in a polymer
US10438662B2 (en) 2016-02-29 2019-10-08 Iridia, Inc. Methods, compositions, and devices for information storage
EP3526603B1 (en) 2016-10-12 2022-07-20 F. Hoffmann-La Roche AG Nanopore voltage methods
EP3526349B1 (en) 2016-10-13 2021-03-17 F. Hoffmann-La Roche AG Molecular detection and counting using nanopores
JP7277054B2 (en) 2017-05-31 2023-05-18 モレキュラー アッセンブリーズ， インコーポレイテッド Homopolymer-encoded nucleic acid memory
US11612873B2 (en) 2017-05-31 2023-03-28 Molecular Assemblies, Inc. Homopolymer encoded nucleic acid memory
US11174512B2 (en) * 2017-05-31 2021-11-16 Molecular Assemblies, Inc. Homopolymer encoded nucleic acid memory
LU100392B1 (en) * 2017-07-27 2019-02-12 Wisconsin Alumni Res Foundation Warf A method to determine the throughput speed of a pore
JP2021528094A (en) 2018-06-29 2021-10-21 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ Detection of microsatellite instability
CN110610053B (en) * 2019-09-20 2023-04-28 广东技术师范大学 Method for testing service life of deceleration strip
FR3110177A1 (en) * 2020-05-15 2021-11-19 Université De Bretagne Sud method and device for decoding data stored in a DNA-based storage system
US11837302B1 (en) 2020-08-07 2023-12-05 Iridia, Inc. Systems and methods for writing and reading data stored in a polymer using nano-channels
EP4294941A1 (en) 2021-02-18 2023-12-27 F. Hoffmann-La Roche AG Structure to prevent threading of nucleic acid templates through a nanopore during sequencing
WO2023092139A2 (en) * 2021-11-22 2023-05-25 The Trustees Of Columbia University In The City Of New York Systems and methods for isolation of desired nucleic acid strands

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050186576A1 (en) * 2004-02-19 2005-08-25 Intel Corporation Polymer sequencing using selectively labeled monomers and data integration
US6952651B2 (en) * 2002-06-17 2005-10-04 Intel Corporation Methods and apparatus for nucleic acid sequencing by signal stretching and data integration
US20090099786A1 (en) * 2007-10-01 2009-04-16 Nabsys, Inc. Biopolymer sequencing by hybridization of probes to form ternary complexes and variable range alignment
US20100243449A1 (en) * 2009-03-27 2010-09-30 Oliver John S Devices and methods for analyzing biomolecules and probes bound thereto

Family Cites Families (192)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2656508A (en) 1949-08-27 1953-10-20 Wallace H Coulter Means for counting particles suspended in a fluid
US4121192A (en) 1974-01-31 1978-10-17 Gte Sylvania Incorporated System and method for determining position and velocity of an intruder from an array of sensors
US4859945A (en) 1988-05-03 1989-08-22 Elscint Ltd. Optimized signal to noise ratio
US5198543A (en) 1989-03-24 1993-03-30 Consejo Superior Investigaciones Cientificas PHI29 DNA polymerase
US5302509A (en) 1989-08-14 1994-04-12 Beckman Instruments, Inc. Method for sequencing polynucleotides
EP0450060A1 (en) 1989-10-26 1991-10-09 Sri International Dna sequencing
JPH04236295A (en) 1991-01-18 1992-08-25 Sharp Corp Ferromagnetic liquid crystal composition and liquid crystal element
DE69305160T2 (en) 1992-04-22 1997-04-30 Ecole Polytech LIPID MEMBRANES FOR BIOSENSOR APPLICATIONS
GB9208733D0 (en) 1992-04-22 1992-06-10 Medical Res Council Dna sequencing method
GB9315847D0 (en) 1993-07-30 1993-09-15 Isis Innovation Tag reagent and assay method
US5457342A (en) 1994-03-30 1995-10-10 Herbst, Ii; Gerhardt G. Integrated circuit cooling apparatus
FR2722294B1 (en) 1994-07-07 1996-10-04 Lyon Ecole Centrale PROCESS FOR THE QUALITATIVE AND / OR QUANTITATIVE ANALYSIS OF BIOLOGICAL SUBSTANCES PRESENT IN A CONDUCTIVE LIQUID MEDIUM AND BIOCHEMICAL AFFINITY SENSORS USED FOR THE IMPLEMENTATION OF THIS PROCESS
JP2986381B2 (en) 1994-08-16 1999-12-06 インターナショナル・ビジネス・マシーンズ・コーポレイション Electronic chip temperature control device and method
US5912155A (en) 1994-09-30 1999-06-15 Life Technologies, Inc. Cloned DNA polymerases from Thermotoga neapolitana
US6014213A (en) 1994-12-12 2000-01-11 Visible Genetics Inc. High dynamic range apparatus for separation and detection of polynucleotide fragments
US6362002B1 (en) 1995-03-17 2002-03-26 President And Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US5795782A (en) 1995-03-17 1998-08-18 President & Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US20120160687A1 (en) 1995-03-17 2012-06-28 President And Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US5780231A (en) 1995-11-17 1998-07-14 Lynx Therapeutics, Inc. DNA extension and analysis with rolling primers
US6261797B1 (en) 1996-01-29 2001-07-17 Stratagene Primer-mediated polynucleotide synthesis and manipulation techniques
WO1997046704A1 (en) 1996-06-06 1997-12-11 Lynx Therapeutics, Inc. Sequencing by ligation of encoded adaptors
US5981733A (en) 1996-09-16 1999-11-09 Incyte Pharmaceuticals, Inc. Apparatus for the chemical synthesis of molecular arrays
US6699719B2 (en) 1996-11-29 2004-03-02 Proteomic Systems, Inc. Biosensor arrays and methods
JPH10187253A (en) 1996-12-27 1998-07-14 Ando Electric Co Ltd Temperature control device for optical semiconductor element
US6046005A (en) 1997-01-15 2000-04-04 Incyte Pharmaceuticals, Inc. Nucleic acid sequencing with solid phase capturable terminators comprising a cleavable linking group
US5804386A (en) 1997-01-15 1998-09-08 Incyte Pharmaceuticals, Inc. Sets of labeled energy transfer fluorescent primers and their use in multi component analysis
US5876936A (en) 1997-01-15 1999-03-02 Incyte Pharmaceuticals, Inc. Nucleic acid sequencing with solid phase capturable terminators
AU743025B2 (en) 1997-03-12 2002-01-17 Applera Corporation DNA polymerases having improved labeled nucleotide incorporation properties
US6002263A (en) 1997-06-06 1999-12-14 Cascade Microtech, Inc. Probe station having inner and outer shielding
WO1999005167A1 (en) 1997-07-25 1999-02-04 University Of Massachusetts Designed protein pores as components for biosensors
SE9703958D0 (en) 1997-10-29 1997-10-29 Pacesetter Ab Method and device for determination of concentration
EP0921196A1 (en) 1997-12-02 1999-06-09 Roche Diagnostics GmbH Modified DNA-polymerase from carboxydothermus hydrogenoformans and its use for coupled reverse transcription and polymerase chain reaction
DE19810879A1 (en) 1998-03-13 1999-09-16 Roche Diagnostics Gmbh New chimeric polymerase with 5'-3'-polymerase activity, and optionally proofreading activity, used for polymerase chain reactions and sequencing
WO1999049082A2 (en) 1998-03-23 1999-09-30 Invitrogen Corporation Modified nucleotides and methods useful for nucleic acid sequencing
US6485703B1 (en) 1998-07-31 2002-11-26 The Texas A&M University System Compositions and methods for analyte detection
US6210896B1 (en) 1998-08-13 2001-04-03 Us Genomics Molecular motors
US6217731B1 (en) 1998-10-21 2001-04-17 Spectrumedix Corporation Method and apparatus for monitoring and displaying the status of a parallel capillary electrophoresis device
US6267872B1 (en) 1998-11-06 2001-07-31 The Regents Of The University Of California Miniature support for thin films containing single channels or nanopores and methods for using same
CA2317743A1 (en) 1998-12-11 2000-06-22 Paul Mansky Sensor array-based system and method for rapid materials characterization
AU2180200A (en) 1998-12-14 2000-07-03 Li-Cor Inc. A heterogeneous assay for pyrophosphate detection
US6082115A (en) 1998-12-18 2000-07-04 National Semiconductor Corporation Temperature regulator circuit and precision voltage reference for integrated circuit
NO986133D0 (en) 1998-12-23 1998-12-23 Preben Lexow Method of DNA Sequencing
US20030054360A1 (en) 1999-01-19 2003-03-20 Larry Gold Method and apparatus for the automated generation of nucleic acid ligands
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US6399335B1 (en) 1999-11-16 2002-06-04 Advanced Research And Technology Institute, Inc. γ-phosphoester nucleoside triphosphates
US6383749B2 (en) 1999-12-02 2002-05-07 Clontech Laboratories, Inc. Methods of labeling nucleic acids for use in array based hybridization assays
EP1255772A2 (en) 2000-02-11 2002-11-13 The Texas A & M University System Biosensor compositions and methods of use
US6616895B2 (en) 2000-03-23 2003-09-09 Advanced Research Corporation Solid state membrane channel device for the measurement and characterization of atomic and molecular sized samples
US6413792B1 (en) 2000-04-24 2002-07-02 Eagle Research Development, Llc Ultra-fast nucleic acid sequencing device and a method for making and using the same
US6936702B2 (en) 2000-06-07 2005-08-30 Li-Cor, Inc. Charge-switch nucleotides
ATE384731T1 (en) 2000-08-03 2008-02-15 Hoffmann La Roche NUCLEIC ACID BINDING COMPOUNDS WITH PYRAZOLO 3, 4-D PYRIMIDINE ANALOGUES OF PURINE-2,6-DIAMINE AND THEIR USE
US7153672B1 (en) 2000-08-30 2006-12-26 University Of Rochester Method of performing reverse transcription reaction using reverse transcriptase encoded by non-LTR retrotransposable element
US6627748B1 (en) 2000-09-11 2003-09-30 The Trustees Of Columbia University In The City Of New York Combinatorial fluorescence energy transfer tags and their applications for multiplex genetic analyses
WO2002022883A1 (en) 2000-09-11 2002-03-21 The Trustees Of Columbia University In The City Of New York Combinatorial fluorescence energy transfer tags and uses thereof
US20060057565A1 (en) 2000-09-11 2006-03-16 Jingyue Ju Combinatorial fluorescence energy transfer tags and uses thereof
ATE356222T1 (en) 2000-10-06 2007-03-15 Univ Columbia MASSIVE PARALLEL METHOD FOR DECODING DNA AND RNA
US20030027140A1 (en) 2001-03-30 2003-02-06 Jingyue Ju High-fidelity DNA sequencing using solid phase capturable dideoxynucleotides and mass spectrometry
US6800933B1 (en) 2001-04-23 2004-10-05 Advanced Micro Devices, Inc. Integrated circuit cooling device
US6686997B1 (en) 2001-08-27 2004-02-03 Raytheon Company Apparatus and a method for pulse detection and characterization
US7052839B2 (en) 2001-08-29 2006-05-30 Amersham Biosciences Corp Terminal-phosphate-labeled nucleotides and methods of use
US7033762B2 (en) 2001-08-29 2006-04-25 Amersham Biosciences Corp Single nucleotide amplification and detection by polymerase
US7727722B2 (en) 2001-08-29 2010-06-01 General Electric Company Ligation amplification
US7256019B2 (en) 2001-08-29 2007-08-14 Ge Healthcare Bio-Sciences Corp. Terminal phosphate blocked nucleoside polyphosphates
US7041812B2 (en) 2001-08-29 2006-05-09 Amersham Biosciences Corp Labeled nucleoside polyphosphates
US7223541B2 (en) 2001-08-29 2007-05-29 Ge Healthcare Bio-Sciences Corp. Terminal-phosphate-labeled nucleotides and methods of use
EP1436385A4 (en) 2001-09-14 2005-12-14 Invitrogen Corp Dna polymerases and mutants thereof
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
CN100575485C (en) 2002-01-23 2009-12-30 犹他大学研究基金会 Use the directed karyomit(e) mutagenesis of Zinc finger nuclease
US20030166282A1 (en) 2002-02-01 2003-09-04 David Brown High potency siRNAS for reducing the expression of target genes
US7948041B2 (en) 2005-05-19 2011-05-24 Nanomix, Inc. Sensor having a thin-film inhibition layer
US7074597B2 (en) 2002-07-12 2006-07-11 The Trustees Of Columbia University In The City Of New York Multiplex genotyping using solid phase capturable dideoxynucleotides and mass spectrometry
DE10238843B8 (en) 2002-08-20 2008-01-03 Infineon Technologies Ag Semiconductor component
US7373199B2 (en) 2002-08-27 2008-05-13 University Of Florida Research Foundation, Inc. Optimization of multi-dimensional time series processing for seizure warning and prediction
US7355216B2 (en) 2002-12-09 2008-04-08 The Regents Of The University Of California Fluidic nanotubes and devices
WO2004055160A2 (en) 2002-12-13 2004-07-01 The Trustees Of Columbia University In The City Of New York Biomolecular coupling methods using 1,3-dipolar cycloaddition chemistry
AU2004211920B2 (en) 2003-02-05 2009-05-14 Global Life Sciences Solutions Usa Llc Solid phase sequencing
EP1592779A4 (en) 2003-02-05 2007-12-12 Ge Healthcare Bio Sciences Terminal-phosphate-labeled nucleotides with new linkers
WO2004085609A2 (en) 2003-02-28 2004-10-07 Brown University Nanopores, methods for using same, methods for making same and methods for characterizing biomolecules using same
WO2004092331A2 (en) 2003-04-08 2004-10-28 Li-Cor, Inc. Composition and method for nucleic acid sequencing
GB0308851D0 (en) 2003-04-16 2003-05-21 Lingvitae As Method
US7521225B2 (en) 2003-06-13 2009-04-21 North Carolina State University Nanotube structures having a surfactant bilayer inner wall coating
US6880345B1 (en) 2003-11-04 2005-04-19 Intel Corporation Cooling system for an electronic component
US7019346B2 (en) 2003-12-23 2006-03-28 Intel Corporation Capacitor having an anodic metal oxide substrate
JP4107269B2 (en) 2004-02-23 2008-06-25 ソニー株式会社 Solid-state imaging device
US7622026B2 (en) 2004-03-02 2009-11-24 Panasonic Corporation Biosensor
WO2005084367A2 (en) 2004-03-03 2005-09-15 The Trustees Of Columbia University In The City Of New York Photocleavable fluorescent nucleotides for dna sequencing on chip constructed by site-specific coupling chemistry
US7279337B2 (en) 2004-03-10 2007-10-09 Agilent Technologies, Inc. Method and apparatus for sequencing polymers through tunneling conductance variation detection
WO2006028508A2 (en) 2004-03-23 2006-03-16 President And Fellows Of Harvard College Methods and apparatus for characterizing polynucleotides
US20050221351A1 (en) 2004-04-06 2005-10-06 Affymetrix, Inc. Methods and devices for microarray image analysis
US20050239134A1 (en) 2004-04-21 2005-10-27 Board Of Regents, The University Of Texas System Combinatorial selection of phosphorothioate single-stranded DNA aptamers for TGF-beta-1 protein
JP2006004079A (en) 2004-06-16 2006-01-05 Sony Corp Storage device
US6880346B1 (en) 2004-07-08 2005-04-19 Giga-Byte Technology Co., Ltd. Two stage radiation thermoelectric cooling apparatus
AT501510B1 (en) 2004-07-19 2009-05-15 Univ Wien Tech DECENTRALIZED ERROR TOLERANT CLOCK GENERATION IN VLSI CHIPS
DE112005001781B4 (en) 2004-07-23 2017-12-14 Electronic Biosciences, Inc. Method and device for measuring a time-variable current flowing through an ion channel with a capacitive measuring electrode
CA2577079C (en) 2004-08-13 2014-05-20 President And Fellows Of Harvard College An ultra high-throughput opti-nanopore dna readout platform
GB0422733D0 (en) 2004-10-13 2004-11-17 Lingvitae As Method
US20060105461A1 (en) 2004-10-22 2006-05-18 May Tom-Moy Nanopore analysis system
EP1842061A4 (en) 2004-12-21 2009-05-13 Texas A & M Univ Sys High temperature ion channels and pores
GB0505971D0 (en) 2005-03-23 2005-04-27 Isis Innovation Delivery of molecules to a lipid bilayer
US7215032B2 (en) 2005-06-14 2007-05-08 Cubic Wafer, Inc. Triaxial through-chip connection
US9169510B2 (en) 2005-06-21 2015-10-27 The Trustees Of Columbia University In The City Of New York Pyrosequencing methods and related compositions
US7645866B2 (en) 2005-06-28 2010-01-12 Life Technologies Corporation Methods of producing and sequencing modified polynucleotides
JP4524652B2 (en) 2005-07-06 2010-08-18 ソニー株式会社 AD converter and semiconductor device
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
WO2007041621A2 (en) 2005-10-03 2007-04-12 Xingsheng Sean Ling Hybridization assisted nanopore sequencing
US7397232B2 (en) 2005-10-21 2008-07-08 The University Of Akron Coulter counter having a plurality of channels
WO2007053719A2 (en) 2005-10-31 2007-05-10 The Trustees Of Columbia University In The City Of New York Chemically cleavable 3'-o-allyl-dntp-allyl-fluorophore fluorescent nucleotide analogues and related methods
WO2007053702A2 (en) 2005-10-31 2007-05-10 The Trustees Of Columbia University In The City Of New York Synthesis of four color 3'-o-allyl modified photocleavable fluorescent nucleotides and related methods
CA2630544A1 (en) 2005-11-21 2007-05-31 The Trustees Of Columbia University In The City Of New York Multiplex digital immuno-sensing using a library of photocleavable mass tags
US7871777B2 (en) 2005-12-12 2011-01-18 The United States Of America As Represented By The Department Of Health And Human Services Probe for nucleic acid sequencing and methods of use
CA2633476C (en) 2005-12-22 2015-04-21 Pacific Biosciences Of California, Inc. Active surface coupled polymerases
EP3056575B1 (en) 2005-12-22 2017-10-11 Pacific Biosciences Of California, Inc. Polymerases for nucleotide analogue incorporation
US8002712B2 (en) 2006-01-26 2011-08-23 Meka Vikas V Breath and breath condensate analysis system and associated methods
US7368668B2 (en) 2006-02-03 2008-05-06 Freescale Semiconductor Inc. Ground shields for semiconductors
EP1991110B1 (en) 2006-03-09 2018-11-07 DexCom, Inc. Systems and methods for processing analyte sensor data
US20070298511A1 (en) 2006-04-27 2007-12-27 The Texas A&M University System Nanopore sensor system
US7777505B2 (en) 2006-05-05 2010-08-17 University Of Utah Research Foundation Nanopore platforms for ion channel recordings and single molecule detection and analysis
US7446017B2 (en) 2006-05-31 2008-11-04 Freescale Semiconductor, Inc. Methods and apparatus for RF shielding in vertically-integrated semiconductor devices
WO2007146158A1 (en) 2006-06-07 2007-12-21 The Trustees Of Columbia University In The City Of New York Dna sequencing by nanopore using modified nucleotides
BRPI0603825A (en) 2006-09-19 2008-05-06 Syngenta Protecao De Cultivos sprout growth regulator composition in tobacco plantations, method of controlling sprout growth in tobacco plantations and use of an effective amount of flumetralin, at least one solvent and at least one surfactant
WO2008051530A2 (en) 2006-10-23 2008-05-02 Pacific Biosciences Of California, Inc. Polymerase enzymes and reagents for enhanced nucleic acid sequencing
DE112007002932B4 (en) 2006-12-01 2015-08-06 The Trustees Of Columbia University In The City Of New York Four-color DNA sequencing by synthesis using cleavable, reversible, fluorescent nucleotide terminators
US7902345B2 (en) 2006-12-05 2011-03-08 Sequenom, Inc. Detection and quantification of biomolecules using mass spectrometry
CN101669026B (en) 2006-12-14 2014-05-07 生命技术公司 Methods and apparatus for measuring analytes using large scale FET arrays
US8003319B2 (en) 2007-02-02 2011-08-23 International Business Machines Corporation Systems and methods for controlling position of charged polymer inside nanopore
US20100196203A1 (en) 2007-02-20 2010-08-05 Gurdial Singh Sanghera Formation of Lipid Bilayers
CN101680873B (en) 2007-04-04 2015-11-25 加利福尼亚大学董事会 Use composition, equipment, the system and method for nano-pore
EP2158476B8 (en) 2007-05-08 2019-10-09 Trustees of Boston University Chemical functionalization of solid-state nanopores and nanopore arrays and applications thereof
WO2008157696A2 (en) 2007-06-19 2008-12-24 Stratos Genomics Inc. High throughput nucleic acid sequencing by expansion
US20090073293A1 (en) 2007-06-27 2009-03-19 Yoel Yaffe CMOS image sensors with increased dynamic range and methods of operating the same
JP4929090B2 (en) 2007-07-26 2012-05-09 パナソニック株式会社 Solid-state imaging device and driving method thereof
US8273532B2 (en) 2007-10-02 2012-09-25 President And Fellows Of Harvard College Capture, recapture, and trapping of molecules with a nanopore
DK2725107T3 (en) 2007-10-19 2019-01-02 Univ Columbia DNA Sequencing with Non-Fluorescent Nucleotide Reversible Terminators and ddNTPs Modified by Split Label and Nucleic Acid comprising Inosine with Reversible Terminators
EP2207900B1 (en) 2007-10-19 2015-04-29 The Trustees of Columbia University in the City of New York Design and synthesis of cleavable fluorescent nucleotides as reversible terminators for dna sequencing by synthesis
WO2009055617A1 (en) 2007-10-23 2009-04-30 Stratos Genomics Inc. High throughput nucleic acid sequencing by spacing
GB0724736D0 (en) 2007-12-19 2008-01-30 Oxford Nanolabs Ltd Formation of layers of amphiphilic molecules
WO2009091847A2 (en) 2008-01-14 2009-07-23 Life Technologies Corporation Compositions, methods and systems for single molecule sequencing
US7989928B2 (en) 2008-02-05 2011-08-02 Advanced Semiconductor Engineering Inc. Semiconductor device packages with electromagnetic interference shielding
US8022511B2 (en) 2008-02-05 2011-09-20 Advanced Semiconductor Engineering, Inc. Semiconductor device packages with electromagnetic interference shielding
WO2009102470A2 (en) 2008-02-12 2009-08-20 Pacific Biosciences Of California, Inc. Compositions and methods for use in analytical reactions
US8652781B2 (en) 2008-02-12 2014-02-18 Pacific Biosciences Of California, Inc. Cognate sampling kinetics
US20090215050A1 (en) 2008-02-22 2009-08-27 Robert Delmar Jenison Systems and methods for point-of-care amplification and detection of polynucleotides
US7973146B2 (en) 2008-03-26 2011-07-05 Pacific Biosciences Of California, Inc. Engineered fluorescent dye labeled nucleotide analogs for DNA sequencing
US8420366B2 (en) 2008-03-31 2013-04-16 Pacific Biosciences Of California, Inc. Generation of modified polymerases for improved accuracy in single molecule sequencing
EP2942404B1 (en) 2008-03-31 2016-11-23 Pacific Biosciences of California, Inc. Generation of modified polymerases for improved accuracy in single molecule sequencing
US9127259B2 (en) 2008-03-31 2015-09-08 Pacific Biosciences Of California, Inc. Enzymes resistant to photodamage
US8133672B2 (en) 2008-03-31 2012-03-13 Pacific Biosciences Of California, Inc. Two slow-step polymerase enzyme systems and methods
ATE530497T1 (en) 2008-03-31 2011-11-15 Sony Deutschland Gmbh METHOD FOR PRODUCING A MEMBRANE WITH A CONICAL PORE
US8999676B2 (en) 2008-03-31 2015-04-07 Pacific Biosciences Of California, Inc. Recombinant polymerases for improved single molecule sequencing
US8058414B2 (en) 2008-04-29 2011-11-15 Life Technologies Corporation Unnatural polymerase substrates that can sustain enzymatic synthesis of double stranded nucleic acids from a nucleic acid template and methods of use
US8940142B2 (en) 2008-05-05 2015-01-27 The Regents Of The University Of California Functionalized nanopipette biosensor
US20100122907A1 (en) 2008-05-06 2010-05-20 Government of the United States of America, Single molecule mass or size spectrometry in solution using a solitary nanopore
US7906371B2 (en) 2008-05-28 2011-03-15 Stats Chippac, Ltd. Semiconductor device and method of forming holes in substrate to interconnect top shield and ground shield
US20100025238A1 (en) 2008-07-31 2010-02-04 Medtronic Minimed, Inc. Analyte sensor apparatuses having improved electrode configurations and methods for making and using them
CA2735979A1 (en) 2008-09-05 2010-03-11 Pacific Biosciences Of California, Inc. Engineering polymerases and reaction conditions for modified incorporation properties
US8481264B2 (en) 2008-09-19 2013-07-09 Pacific Biosciences Of California, Inc. Immobilized nucleic acid complexes for sequence analysis
US8921046B2 (en) 2008-09-19 2014-12-30 Pacific Biosciences Of California, Inc. Nucleic acid sequence analysis
US8063469B2 (en) 2008-09-30 2011-11-22 Infineon Technologies Ag On-chip radio frequency shield with interconnect metallization
US20100301398A1 (en) 2009-05-29 2010-12-02 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
CA2741996A1 (en) 2008-10-29 2010-05-14 Trustees Of Boston University Sequence preserved dna conversion
US8486630B2 (en) 2008-11-07 2013-07-16 Industrial Technology Research Institute Methods for accurate sequence data and modified base position determination
US20100148126A1 (en) 2008-11-26 2010-06-17 Board Of Regents, The University Of Texas System Genomic sequencing using modified protein pores and ionic liquids
US9156037B2 (en) 2009-01-15 2015-10-13 Children's Medical Center Corporation Microfluidic device and uses thereof
GB0905140D0 (en) 2009-03-25 2009-05-06 Isis Innovation Method
WO2010117470A2 (en) * 2009-04-10 2010-10-14 Pacific Biosciences Of California, Inc. Nanopore sequencing devices and methods
US8926904B2 (en) 2009-05-12 2015-01-06 Daniel Wai-Cheong So Method and apparatus for the analysis and identification of molecules
WO2011038241A1 (en) 2009-09-25 2011-03-31 President And Fellows Of Harvard College Nucleic acid amplification and sequencing by synthesis with fluorogenic nucleotides
AU2010301128B2 (en) 2009-09-30 2014-09-18 Quantapore, Inc. Ultrafast sequencing of biological polymers using a labeled nanopore
CN102741430B (en) 2009-12-01 2016-07-13 牛津楠路珀尔科技有限公司 Biochemical analyzer, for first module carrying out biochemical analysis and associated method
US8748091B2 (en) * 2009-12-18 2014-06-10 The Board Of Trustees Of The University Of Illinois Characterizing stretched polynucleotides in a synthetic nanopassage
JP5858931B2 (en) 2010-02-08 2016-02-10 ジニア・テクノロジーズ・インコーポレーテッドＧｅｎｉａ Ｔｅｃｈｎｏｌｏｇｉｅｓ Ｉｎｃｏｒｐｏｒａｔｅｄ Systems and methods for manipulating molecules in nanopores
US9605307B2 (en) 2010-02-08 2017-03-28 Genia Technologies, Inc. Systems and methods for forming a nanopore in a lipid bilayer
US8324914B2 (en) 2010-02-08 2012-12-04 Genia Technologies, Inc. Systems and methods for characterizing a molecule
US20110287414A1 (en) 2010-02-08 2011-11-24 Genia Technologies, Inc. Systems and methods for identifying a portion of a molecule
US20120052188A1 (en) 2010-02-08 2012-03-01 Genia Technologies, Inc. Systems and methods for assembling a lipid bilayer on a substantially planar solid surface
US20110192723A1 (en) 2010-02-08 2011-08-11 Genia Technologies, Inc. Systems and methods for manipulating a molecule in a nanopore
EP3933402A1 (en) 2010-02-23 2022-01-05 University of Washington Method of making unsupported artificial mycolic acid membranes
US8652779B2 (en) 2010-04-09 2014-02-18 Pacific Biosciences Of California, Inc. Nanopore sequencing using charge blockade labels
EP3190193A1 (en) 2010-07-14 2017-07-12 The Curators Of The University Of Missouri Nanopore-facilitated single molecule detection of nucleic acids
CN107083421A (en) 2010-12-17 2017-08-22 纽约哥伦比亚大学理事会 The DNA detected using the nucleotides through modification and nano-pore is sequenced in synthesis
WO2012088341A2 (en) 2010-12-22 2012-06-28 Genia Technologies, Inc. Nanopore-based single dna molecule characterization, identification and isolation using speed bumps
US8962242B2 (en) 2011-01-24 2015-02-24 Genia Technologies, Inc. System for detecting electrical properties of a molecular complex
US9110478B2 (en) 2011-01-27 2015-08-18 Genia Technologies, Inc. Temperature regulation of measurement arrays
WO2012121756A1 (en) 2011-03-04 2012-09-13 Quantapore, Inc. Apparatus and methods for performing optical nanopore detection or sequencing
US20130244340A1 (en) 2012-01-20 2013-09-19 Genia Technologies, Inc. Nanopore Based Molecular Detection and Sequencing
US8541849B2 (en) 2012-02-14 2013-09-24 Genia Technologies, Inc. Noise shielding techniques for ultra low current measurements in biochemical applications
US8986629B2 (en) 2012-02-27 2015-03-24 Genia Technologies, Inc. Sensor circuit for controlling, detecting, and measuring a molecular complex
US10029915B2 (en) 2012-04-04 2018-07-24 International Business Machines Corporation Functionally switchable self-assembled coating compound for controlling translocation of molecule through nanopores
ES2906186T3 (en) 2012-04-09 2022-04-13 Univ Columbia Method for the preparation of nanopore and uses thereof
CA2876095A1 (en) 2012-06-15 2013-12-19 Genia Technologies, Inc. Chip set-up and high-accuracy nucleic acid sequencing
EP3674412A1 (en) 2012-06-20 2020-07-01 The Trustees of Columbia University in the City of New York Nucleic acid sequencing by nanopore detection of tag molecules
US9605309B2 (en) 2012-11-09 2017-03-28 Genia Technologies, Inc. Nucleic acid sequencing using tags

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6952651B2 (en) * 2002-06-17 2005-10-04 Intel Corporation Methods and apparatus for nucleic acid sequencing by signal stretching and data integration
US20050186576A1 (en) * 2004-02-19 2005-08-25 Intel Corporation Polymer sequencing using selectively labeled monomers and data integration
US20090099786A1 (en) * 2007-10-01 2009-04-16 Nabsys, Inc. Biopolymer sequencing by hybridization of probes to form ternary complexes and variable range alignment
US20100243449A1 (en) * 2009-03-27 2010-09-30 Oliver John S Devices and methods for analyzing biomolecules and probes bound thereto

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
DERRINGTON, I. M. ET AL.: "Nanopore DNA sequencing with MspA", PROC NATL ACAD SCI USA, vol. 107, no. 37, 14 September 2010 (2010-09-14), pages 16060 - 16065 *

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