WO2012095748A3 - Single tube quantitative polymerase chain reaction (pcr) - Google Patents

Single tube quantitative polymerase chain reaction (pcr) Download PDF

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Publication number
WO2012095748A3
WO2012095748A3 PCT/IB2012/000186 IB2012000186W WO2012095748A3 WO 2012095748 A3 WO2012095748 A3 WO 2012095748A3 IB 2012000186 W IB2012000186 W IB 2012000186W WO 2012095748 A3 WO2012095748 A3 WO 2012095748A3
Authority
WO
WIPO (PCT)
Prior art keywords
pcr
polymerase chain
chain reaction
single tube
quantitative polymerase
Prior art date
Application number
PCT/IB2012/000186
Other languages
French (fr)
Other versions
WO2012095748A2 (en
Inventor
Jay Lewington
Rohit MISTRY
Original Assignee
Smiths Detection - Watford Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Smiths Detection - Watford Limited filed Critical Smiths Detection - Watford Limited
Publication of WO2012095748A2 publication Critical patent/WO2012095748A2/en
Publication of WO2012095748A3 publication Critical patent/WO2012095748A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism

Abstract

Provided herein are systems and methods for quantitatively monitoring target amplicons produced by polymerase chain reaction (PCR). In particular, quantitative monitoring of target amplicon(s) in a single-tube PCR reaction without separate calibration reactions are provided.
PCT/IB2012/000186 2011-01-14 2012-01-13 Single tube quantitative polymerase chain reaction (pcr) WO2012095748A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161432859P 2011-01-14 2011-01-14
US61/432,859 2011-01-14

Publications (2)

Publication Number Publication Date
WO2012095748A2 WO2012095748A2 (en) 2012-07-19
WO2012095748A3 true WO2012095748A3 (en) 2012-09-07

Family

ID=45852612

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2012/000186 WO2012095748A2 (en) 2011-01-14 2012-01-13 Single tube quantitative polymerase chain reaction (pcr)

Country Status (1)

Country Link
WO (1) WO2012095748A2 (en)

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5789153A (en) * 1994-09-26 1998-08-04 Immuno Aktiengesellschaft Method of quantitating nucleic acid
US6090552A (en) * 1996-07-16 2000-07-18 Intergen Company Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US20030064401A1 (en) * 2001-08-31 2003-04-03 Centre For Addiction And Mental Health Involvement of the BDNF gene in mood disorders
WO2006044994A2 (en) * 2004-10-18 2006-04-27 Brandeis University Primers, probes and methods for nucleic acid amplification
WO2011019837A1 (en) * 2009-08-11 2011-02-17 Brandeis University Single probe, multiple temperature, nucleic acid detection methods, kits, and compositions
WO2012048207A2 (en) * 2010-10-07 2012-04-12 Brandeis University Compositions and methods for nucleic acid based diagnostic assays

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US5066584A (en) 1988-09-23 1991-11-19 Cetus Corporation Methods for generating single stranded dna by the polymerase chain reaction
US7198897B2 (en) 2001-12-19 2007-04-03 Brandeis University Late-PCR

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5789153A (en) * 1994-09-26 1998-08-04 Immuno Aktiengesellschaft Method of quantitating nucleic acid
US6090552A (en) * 1996-07-16 2000-07-18 Intergen Company Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US20030064401A1 (en) * 2001-08-31 2003-04-03 Centre For Addiction And Mental Health Involvement of the BDNF gene in mood disorders
WO2006044994A2 (en) * 2004-10-18 2006-04-27 Brandeis University Primers, probes and methods for nucleic acid amplification
WO2011019837A1 (en) * 2009-08-11 2011-02-17 Brandeis University Single probe, multiple temperature, nucleic acid detection methods, kits, and compositions
WO2012048207A2 (en) * 2010-10-07 2012-04-12 Brandeis University Compositions and methods for nucleic acid based diagnostic assays

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
ANONYMOUS: "Applied Biosystems 7500/7500 Fast Real-Time PCR System: Presence/Absence Experiments", 1 June 2010 (2010-06-01), pages 1 - 120, XP055027355, Retrieved from the Internet <URL:http://www3.appliedbiosystems.com/cms/groups/mcb_support/documents/generaldocuments/cms_050341.pdf> [retrieved on 20120515] *
KENNETH E PIERCE ET AL: "Linear-After-The-Exponential (LATE)-PCR: Primer design criteria for high yields of specific single-stranded DNA and improved real-time detection", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, NATIONAL ACADEMY OF SCIENCES, vol. 102, no. 24, 14 June 2005 (2005-06-14), pages 8609 - 8614, XP008102902, ISSN: 0027-8424, [retrieved on 20050607], DOI: 10.1073/PNAS.0501946102 *
MICHAEL T. SEIPP ET AL: "Unlabeled Oligonucleotides as Internal Temperature Controls for Genotyping by Amplicon Melting", THE JOURNAL OF MOLECULAR DIAGNOSTICS, vol. 9, no. 3, 1 July 2007 (2007-07-01), pages 284 - 289, XP055027457, ISSN: 1525-1578, DOI: 10.2353/jmoldx.2007.060136 *
PRYOR ROBERT J ET AL: "Real-time polymerase chain reaction and melting curve analysis", METHODS IN MOLECULAR BIOLOGY, HUMANA PRESS INC, NJ, US, vol. 336, no. 3, 1 January 2006 (2006-01-01), pages 19 - 32, XP002594646, ISSN: 1064-3745 *
REBRIKOV D V ET AL: "Real-time PCR: A review of approaches to data analysis", APPLIED BIOCHEMISTRY AND MICROBIOLOGY, KLUWER ACADEMIC PUBLISHERS-PLENUM PUBLISHERS, NE, vol. 42, no. 5, 1 September 2006 (2006-09-01), pages 455 - 463, XP019389693, ISSN: 1608-3024, DOI: 10.1134/S0003683806050024 *
SANCHEZ J AQUILES ET AL: "Two-temperature LATE-PCR endpoint genotyping", BMC BIOTECHNOLOGY, BIOMED CENTRAL LTD. LONDON, GB, vol. 6, no. 1, 4 December 2006 (2006-12-04), pages 44, XP021023580, ISSN: 1472-6750, DOI: 10.1186/1472-6750-6-44 *

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