WO2012176977A3 - Ifret용 탐침을 이용한 단백질 검출 또는 이미지화 현미경 장치 및 이를 이용한 단백질 검출 또는 이미징방법 - Google Patents

Ifret용 탐침을 이용한 단백질 검출 또는 이미지화 현미경 장치 및 이를 이용한 단백질 검출 또는 이미징방법 Download PDF

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Publication number
WO2012176977A3
WO2012176977A3 PCT/KR2012/003126 KR2012003126W WO2012176977A3 WO 2012176977 A3 WO2012176977 A3 WO 2012176977A3 KR 2012003126 W KR2012003126 W KR 2012003126W WO 2012176977 A3 WO2012176977 A3 WO 2012176977A3
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WO
WIPO (PCT)
Prior art keywords
light
detecting
ifret
probe
target protein
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PCT/KR2012/003126
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English (en)
French (fr)
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WO2012176977A2 (ko
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정상전
강효진
김주환
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한국생명공학연구원
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Priority to JP2014516889A priority Critical patent/JP2014520275A/ja
Priority to US14/128,560 priority patent/US20140170769A1/en
Priority to EP12802701.8A priority patent/EP2725356A4/en
Publication of WO2012176977A2 publication Critical patent/WO2012176977A2/ko
Publication of WO2012176977A3 publication Critical patent/WO2012176977A3/ko

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels

Abstract

본 발명에 따른 고유 형광 공명 에너지 전이(iFRET)용 탐침을 이용한 표적 단백질 검출 또는 이미지화 현미경 장치는, 표적 단백질 내 아미노산을 여기하는 파장대의 제1 광 및 iFRET용 탐침의 형광분자를 여기하는 파장대의 제2 광을 조사하는 광 조사부; 상기 광 조사부에서 조사되는 광을 iFRET용 탐침이 도입된 시료 상에 입사하게 하는 대물 렌즈; 및 표적단백질 내 아미노산을 여기하는 파장대의 제1 광을 시료 상에 조사하여 iFRET 용 탐침에서 생성되는 제1 발광 신호와, iFRET용 탐침의 형광분자를 여기하는 파장대의 제2 광을 조사하여 iFRET 용 탐침에서 생성되는 제2 발광 신호를 검출하는 인식부를 포함하며, 여기서 iFRET용 탐침은 표적 단백질에 특이적인 결합 부위(binding site) 또는 상기 결합부위를 갖는 분자; 및 표적 단백질의 고유 형광(intrinsic fluorescence)에 대한 받게(acceptor)기능을 갖는 형광 분자가 직접 또는 링커를 통해 결합된 것이다.
PCT/KR2012/003126 2011-06-23 2012-04-23 Ifret용 탐침을 이용한 단백질 검출 또는 이미지화 현미경 장치 및 이를 이용한 단백질 검출 또는 이미징방법 WO2012176977A2 (ko)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP2014516889A JP2014520275A (ja) 2011-06-23 2012-04-23 ｉＦＲＥＴ用探針を利用してタンパク質を検出又はイメージ化する顕微鏡装置及びこれを利用したタンパク質の検出又はイメージング方法
US14/128,560 US20140170769A1 (en) 2011-06-23 2012-04-23 Microscope apparatus for detecting or imaging protein using probe for intrinsic fluorescence resonance energy transfer and method for detecting or imaging protein using the same
EP12802701.8A EP2725356A4 (en) 2011-06-23 2012-04-23 MICROSCOPE-TYPE APPARATUS FOR DETECTING OR IMAGING A PROTEIN USING AN IFRET PROBE AND METHOD OF DETECTING OR IMAGING PROTEIN EMPLOYING IT

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR10-2011-0061410 2011-06-23
KR20110061410 2011-06-23
KR1020120042318A KR101320097B1 (ko) 2011-06-23 2012-04-23 고유 형광 공명 에너지 전이용 탐침을 이용한 단백질 검출 또는 이미지화 현미경 장치 및 이를 이용한 단백질 검출 또는 이미징방법
KR10-2012-0042318 2012-04-23

Publications (2)

Publication Number Publication Date
WO2012176977A2 WO2012176977A2 (ko) 2012-12-27
WO2012176977A3 true WO2012176977A3 (ko) 2013-02-14

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PCT/KR2012/003126 WO2012176977A2 (ko) 2011-06-23 2012-04-23 Ifret용 탐침을 이용한 단백질 검출 또는 이미지화 현미경 장치 및 이를 이용한 단백질 검출 또는 이미징방법

Country Status (5)

Country Link
US (1) US20140170769A1 (ko)
EP (1) EP2725356A4 (ko)
JP (1) JP2014520275A (ko)
KR (1) KR101320097B1 (ko)
WO (1) WO2012176977A2 (ko)

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* Cited by examiner, † Cited by third party
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US20140242606A1 (en) * 2011-06-23 2014-08-28 Korea Research Institute Of Bioscience And Biotechnology PROBE FOR iFRET AND USE THEREOF
JP6277931B2 (ja) * 2014-10-01 2018-02-14 信越半導体株式会社 貼り合わせ不良部の検出方法及び検査システム
CN108107562A (zh) * 2018-02-06 2018-06-01 福州大学 一种基于智能手机的便携式荧光显微镜及其工作方法
KR20230144199A (ko) 2022-04-07 2023-10-16 가톨릭대학교 산학협력단 약물 전달체 및 이의 제조 방법

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US20040146913A1 (en) * 2003-01-09 2004-07-29 Hamamatsu Photonics K.K. Fluorescence resonance energy transfer analyzer
JP2008504517A (ja) * 2004-06-28 2008-02-14 シ ビオ アンテルナショナル 共鳴エネルギー移動中における蛍光の信号検出を改善する方法
JP2006030178A (ja) * 2004-07-16 2006-02-02 Carl Zeiss Jena Gmbh 高度な分解能を持つ顕微鏡
KR20110005025U (ko) * 2009-11-13 2011-05-19 한국생명공학연구원 고감도 휴대용 ｆｒｅｔ 형광측정 장치

Also Published As

Publication number Publication date
WO2012176977A2 (ko) 2012-12-27
EP2725356A2 (en) 2014-04-30
EP2725356A4 (en) 2014-12-03
KR101320097B1 (ko) 2013-10-18
KR20130028625A (ko) 2013-03-19
JP2014520275A (ja) 2014-08-21
US20140170769A1 (en) 2014-06-19

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