WO2013012256A2

WO2013012256A2 - Brush and brushgun for collecting cervical cells

Info

Publication number: WO2013012256A2
Application number: PCT/KR2012/005746
Authority: WO
Inventors: 문상호
Original assignee: 주식회사 셀앤텍바이오
Priority date: 2011-07-19
Filing date: 2012-07-18
Publication date: 2013-01-24
Also published as: KR101107554B1; WO2013012256A3

Abstract

The present invention relates to a brush for efficiently collecting cervical cells, comprising: bristles for collecting endocervical cells, formed at a center of an upper surface of a support; bristles for collecting ectocervical cells, formed in plurality at both sides of the bristles for collecting endocervical cells on the support; and a connection neck which protrudes from a lower surface of the support and which has a connection groove formed at a lower surface thereof. The bristles for collecting ectocervical cells consist of: one or more outer bristles formed at an edge of the support; and inner bristles which are formed inwardly from the outer bristles and which have a length shorter than that of the outer bristles.

Description

Cervical Cell Collection Brushes and Brush Guns

The present invention relates to brushes and brush guns, and more particularly, to brushes and brush guns for cervical cell harvesting.

In general, the collection of cells of the cervix is necessary for the diagnosis of diseases related to the genitals, particularly the uterus, in which the cell collection brush is commonly used.

However, in the conventional brush, the structure of the part inserted into the endometrium of the cervix is not suitable for collecting cervical endometrial cells, and the overall shape is incompatible with the three-dimensional structure of the cervix. (bleeding) is likely. When the bleeding occurs in the uterine cancer test, the red blood cells (RBC) to cover the normal cells and cancer cells is a problem that is difficult to read. In addition, the conventional brush is different in the power to collect the cells according to the person used, so fewer cells are collected or cause bleeding to make cancer cells invisible, so it is always to collect a constant cell, bleeding does not occur and intrauterine lesions There is a need for a brush gun to make it look good.

In order to overcome the drawbacks described above, various cell collection brushes have been proposed. Korean Patent Laid-Open Publication No. 2004-0082781 (hereinafter referred to as Document 1) is formed in a line with a handle portion and the handle portion. Disclosed is a cervical cell harvesting device comprising a harvesting unit having a first harvesting table and a collecting brush made of fine strands surrounding the first harvesting table. In particular, Document 1 also discloses a cell collection brush having a second collection stage formed in the shape of a bow for collecting the cells of the cervical outer membrane in addition to the first collection stage for collecting the cells of the cervical lining.

However, in the case of the brush for collecting cells disclosed in Document 1, since the collecting brush made of fine strand material has a normal washing brush shape surrounding the collecting table around 360 degrees, there is a limit to the number of cells collected per unit brush. Since only a portion of the cells are smeared on the slide glass after smearing, the loss of the sample is large, and the production cost is increased because it cannot be manufactured by a conventional mold manufacturing method, such as having to prepare a sampling brush in a separate process.

The present invention is to solve various problems, including the above problems, an object of the present invention to provide a brush for cervical cell collection and a brush gun for operating the brush. However, these problems are exemplary, and the scope of the present invention is not limited thereby.

According to one aspect of the invention, the uterine cervix harvesting hair formed in the central portion on the support zone; A uterine outer cervix collecting hair formed in plural on both sides of the uterine cervical collecting hair on the support; And a connecting neck protruding toward the lower surface of the support and having a connection groove provided at the bottom thereof, wherein the cervical outer neck collecting hair has at least one outer hair and the outer hair formed at the edge of the support. There is provided a cervical cell harvesting brush, consisting of a medial hair having a shorter length than the lateral hair formed.

At this time, the uterine cervix collecting hair may be formed in the same direction as the uterine uterine neck collecting hair, the length of which may be the same as, or longer or shorter than the length of the outer hair. Optionally, the uterine cervical harvesting hair may be formed on both sides of the uterine cervical harvesting rod that protrudes in the same direction as the uterine outer cervical hair harvesting instead of being formed directly on the support. In this case, the uterine cervical harvesting hair may be formed 180 degrees between the hair formed on one side and the hair formed on the other side, it may be more preferable to form a right angle with respect to the longitudinal direction of the support. In addition, a protective cap for the protection of the uterine cervix may be added to the end of the uterine cervical sampling rod, the protective cap may be disk-shaped or hemispherical.

In addition, the inner hair may have the same length and may become shorter from the outer side to the inner side.

The connecting groove may be connected to the connection portion of the handle or brush gun connected to the brush, but may be formed in a polygonal, pentagonal or hexagonal star, straight or cross shape so as to rotate together with the rotation of the handle or brush gun.

In addition, the connection neck may be formed on the outer circumferential surface of the connection neck for binding with the brush gun.

In another aspect of the invention, the rotary rod having a coupling portion capable of coupling with the cervical cell collection brush of claim 1 at one end; A trigger that is interlocked with the other end of the rotary rod to enable rotation and retraction of the rotary rod; A main body having a handle and surrounding an interlocking portion of the rotating rod and the trigger; And a brush detachment unit which is formed outside the rotating rod and pushes and detaches the brush for cervical cell harvesting, and a brush detaching switch which interlocks with the brush detaching unit and drives the brush detaching unit. Is provided.

In this case, the brush detachable part and the brush detachable switch may be integrally formed.

The rotating rod may rotate forward and in a predetermined direction when the trigger is pulled, and may rotate in a reverse and opposite direction when the trigger is returned.

In addition, the brush gun according to an embodiment of the present invention is a lighting device capable of supplying light in a direction parallel to the longitudinal direction of the rotating rod, a power supply for supplying electricity to the lighting device and to control the operation of the power supply device It may further include a power switch.

In addition, the rotary rod and the trigger may be mechanically interlocked or electrically interlocked.

When the rotary rod and the trigger are electrically interlocked, the rotary rod may further include an electric motor for driving the rotary rod. In addition, in this case, a speed adjusting device for adjusting the rotation speed may be further provided.

According to one embodiment of the present invention made as described above, it is possible to efficiently collect cells of the cervix while minimizing bleeding in the uterus of the subject. Of course, the scope of the present invention is not limited by these effects.

1 is a perspective view (a) and a bottom view (b) schematically showing a cervical cell harvesting brush according to an embodiment of the present invention.

2 to 4 is a front view schematically showing another embodiment of the cervical cell harvesting brush of the present invention.

5 is a schematic diagram schematically showing a brush gun according to an embodiment of the present invention.

6 is a state diagram schematically showing how the brush according to an embodiment of the present invention is attached to the brush gun according to an embodiment of the present invention.

Figure 7 is a state diagram schematically showing the appearance after use of the cervical cell harvesting brush and brush gun according to an embodiment of the present invention.

8 is a state diagram schematically showing the appearance after use of the cervical cell harvesting brush according to an embodiment of the present invention.

Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings. However, the present invention is not limited to the embodiments disclosed below, but can be implemented in various forms, and the following embodiments are intended to complete the disclosure of the present invention, the scope of the invention to those skilled in the art It is provided to inform you completely. In addition, the components may be exaggerated or reduced in size in the drawings for convenience of description.

In the following embodiments, the x-axis, y-axis and z-axis are not limited to three axes on the Cartesian coordinate system, but may be interpreted in a broad sense including the same. For example, the x-axis, y-axis, and z-axis may be orthogonal to each other, but may refer to different directions that are not orthogonal to each other.

1A is a perspective view schematically showing a cervical cell harvesting brush according to an embodiment of the present invention.

As shown in Figure 1a, cervical cell collection brush 10 according to an embodiment of the present invention for collecting a plurality of cervical external cervical neck formed in the vertical direction (z-axis direction) with respect to the upper surface of the support (11). A neck (13) formed at the center of the parent (12) and the support (11) and a neck (13) and a connecting neck protruding for connection with a handle (not shown) or a brush gun (see FIG. 5) at the bottom of the support (11) ( 14).

Cervical cell collection brush according to an embodiment of the present invention is the outer uterine epithelium (12a) for collecting the cells of the uterine epithelium for collecting the cells outside the cervix and the length is shorter than the inside of the cervical epicardium It is a big technical feature that it consists of the inner hair 12b for collecting the cells of the.

This is related to the three-dimensional structure of the cervix, which collects like a half cut apple. In other words, the cervix is an opening of the planar canal, which is a passage connected to the middle uterus, and the outer opening corresponds to a portion with an apple crust, and the outer portion of the cervix has a three-dimensional structure that protrudes toward the vagina rather than a plane.

Therefore, in the case of the conventional long cervical hair and the conventional cervical hair brush that becomes shorter to both sides, it is almost impossible to collect the cells at the outermost part of the cervix, and the hair is concentrated too much on the outer side. This can cause excessive damage to the outer and planar canals.

On the other hand, the cervical cell harvesting brush 10 according to an embodiment of the present invention has a relatively outer uterine cervix where the outer hair 12a extending at both ends of the support 11 is naturally curved toward the outer side of the cervix. It can be sufficiently in contact with the body, and in the case of the short medial hair 12b, it can be in sufficient contact with the relatively protruding inside of the cervix, thereby enabling omnidirectional cell collection to the cervix.

In the center of the support (11) of the cervical cell collection brush 10 according to an embodiment of the present invention is formed a uterine cervical sampling hair 13, the uterine cervical sampling hair 13 is a support (11) ) Are formed on both sides of the uterine cervical sampling rod 15 protruding in the opposite direction of the connecting neck 14 in the longitudinal direction (y-axis direction) and at right angles (z-axis direction). The uterine cervix harvesting hair 13 forms a right angle with the longitudinal direction of the support 11 (y-axis direction) and the formation direction (z-axis direction) of the uterine cervical sampling rod 15 (x-axis direction). This is desirable to maximize the contact area of the brush 10 when plating the cells onto the slide glass after collecting the cells with the brush 10 (see FIG. 8). Optionally, when the cells are suspended in the cell preservation liquid by placing the brush 10 in the cell preservation solution and then indirectly smearing the cells onto the slide glass without the cells being smeared onto the slide glass directly (see FIG. 7). ) The uterine cervix harvesting hair 13 may not be in the x-axis direction but may be in the y-axis direction or the middle direction of the two. End of uterine cervical sampling rod 15 may be a protective cap 18 for protecting the uterine cervix, in addition to the disc shape shown in the drawing so that the end of the uterine cervical sampling rod 15 is not sharp Hemispherical spherical shapes and the like are possible.

1B is a bottom view of the cervical cell harvesting brush 10 according to an embodiment of the present invention. Cervical cell collection brush 10 according to an embodiment of the present invention has a connection neck 14 connected to the handle or brush gun, as shown in Figure 1b, the lower surface of the connection neck handle or brush gun And a connecting groove 16 is formed for connection. The connecting groove may have a cross shape as shown in FIG. 1B, but any shape that may receive a rotational force so that the handle or the brush gun may turn back when the handle gun rotates may be used. Specifically, a polygon such as a triangle, a square, a pentagon or a hexagon, or a star-shaped groove such as a pentagonal star or a hexagonal star or a single-shaped groove is also possible.

In addition, a binding groove 17 for binding the brush lock of the brush gun may be formed on the outer circumferential surface of the connection neck 14.

2 is a front view of the cervical cell harvesting brush 10 'according to another embodiment of the present invention. As shown in FIG. 2, the uterine cervix harvesting hair is the same as that shown in FIG. 1, whereas the uterine cervical harvesting hair is formed in a direction perpendicular to the upper surface of the support 11 (z-axis direction).

3 is a front view of a cervical cell harvesting brush 10 '' according to another embodiment of the present invention. The brush 10 ″ shown in FIG. 3 has an internal uterine cervical cap 13 having the same shape as the brush 10 ′ shown in FIG. Unlike those shown in Figs. 1 and 2, 12b is shorter in length from the outside to the inside.

4 is a front view of a cervical cell harvesting brush 10 ′ ″ according to another embodiment of the present invention. The brush 10 '' 'shown in FIG. 4 has the same uterine shape as the brush 13' shown in FIG. 3 and the uterus having the same shape as the brush 10 '' shown in FIG. The outer diameter part collecting hair 12 is provided.

Material of the cervical

cell harvesting brush

10, 10 ′, 10 ″, 10 ″ ″ according to an embodiment of the present invention is not particularly limited thereto, but may be a flexible material such as silicon. In addition, cervical cell collection brushes 10, 10 ′, 10 ″, 10 ′ ″ according to an embodiment of the present invention may be cast from a single mold to have an integral structure, and then manufactured for each part. It may be assembled.

5 is a schematic diagram schematically showing a brush gun 100 according to an embodiment of the present invention. Brush gun 100 is attached to the cervical cell harvesting brush 10 according to an embodiment of the present invention, and then inserted into the vagina extended by the planar, to easily collect the cells of the outer membrane and inner membrane of the cervix As a device for the purpose, the rotary rod 110 is provided with a coupling portion 111 with the cervical cell collection brush 10 at one end, and the other end of the rotary rod 110 to enable the rotation of the rotary rod 110. The trigger 120, surrounding the interlocking portion 130 of the rotating rod 110 and the trigger 120, the body 140 having a handle 141, formed on the outside of the rotating rod 110 and the cervical cell collection brush Brush detachment unit 142 to push the removable (10) and the brush detachment unit 142 in conjunction with the brush detachment switch 143 for driving it. The interlocking portion 130 transmits the forward and backward movement of the trigger 120 to the rotary rod 110 to enable the rotation of the rotary rod 110. Conversion of the movement of the trigger 110 into the rotation of the rotary rod 110 is a screw 112 at the rear of the rotary rod 110 that is screwed with it when the linking part 130 connected to the trigger 120 is moved back and forth Can be achieved by rotating In order to return the trigger 120, an elastic part (not shown) may be provided, and the elastic part is preferably a spring.

Brush gun 100 according to an embodiment of the present invention may further include a lighting device 150 around the rotating rod 110 to supply the light required to illuminate the cervix, the power supply for the lighting device 150 A power supply device 160 for supplying and a power switch 170 for controlling the opening and closing of the power supply may be further provided. The lighting device 150 may be a small bulb or an LED, but is preferably an LED, and the power supply device 160 may be an external power connection terminal or a battery.

Brush gun 100 according to an embodiment of the present invention is combined with the binding groove 17 of the cervical cell harvesting brush 10 further the brush lock 170 that can strengthen the binding with the brush (10) It can be provided.

In addition, a photographing camera (not shown) may be provided at any one of the places where the lighting device 150 is located. In this case, the brush gun 100 according to an embodiment of the present invention may be manipulated while viewing the screen shot by the camera.

Hereinafter, the operation mode of the brush gun 100 and the cervical cell collection brush 10 according to an embodiment of the present invention will be described in detail with reference to the drawings.

6 is a diagram illustrating a state in which a brush gun 100 according to an embodiment of the present invention is attached to a brush gun 100 according to an embodiment of the present invention. The coupling portion 111 of the brush gun 100 is coupled to the connection groove 16 of the cervical cell collection brush 10, and the brush lock 170 of the brush gun 100 is the cervical cell collection brush 10. When bound and fixed to the binding groove 17 formed on the outer circumferential surface of the connecting neck 14 of the uterine cervix, the cervical cell collection brush 10 fixed to the brush gun 100 is inserted into the vagina extended by the planar diameter. Using light irradiated by the lighting device 150 or the external lighting device provided in the main body 140 of the brush 100, when the cervical part comes into contact with the cervix, carefully collects the cap 13 of the cervix. Will be inserted into When the hair of the brush 10 comes into close contact with the cervix, the operator pulls the trigger 120 in the direction of the handle. When the trigger 120 is pulled, the rotating rod 110 is rotated by the action of the interlocking portion 130. Cells 200 are attached to the outer uterine cervical sampling cap 12 which rotates with the rotation of the rotary rod 110, and likewise, the inner cervical neck cap 13 inserted into the planar canal is rotated by friction during rotation. The detached cells will be attached. When the gripping of the trigger 120 is stopped, the trigger 120 is returned to its original position by the elastic device, and the rotating rod 110 rotates in the opposite direction. After repeating this process several times, the brush gun 100 is retracted and separated from the vaginal opening.

Then, when the brush detaching switch 142 is pressed, the brush lock 170 is released, and the brush detaching part 142 pushes the brush neck 15 so that the cervical cell collection brush 10 is a brush gun 100 Desorption from). The detached cervical cell collection brush 10 is dropped into the cell collection container 300, and then injected into the cell retention solution 400 contained in the cell collection container 300, and the cervix is vortexed. Cells attached to the hair of the cell collection brush 10 are detached (FIG. 7). The detached cells 200 are plated on the slide glass by the liquid cell smearing device (not shown) and then analyzed.

Optionally, before or after detaching the cervical cell harvesting brush 10, as shown in FIG. 8, the cervical cell harvesting brush 10 according to an embodiment of the present invention may be removed from the cervix. Both the collecting hair 12 and the cervical neck collecting hair 13 are positioned to contact the slide glass 500, and then moved in the slide length direction to collect the outer cervical neck hair 12 and the cervical neck. Cells 200 attached to the parent 13 may be transferred onto the slide glass 500. In this case, there is an advantage that the origin of the attached cells can be confirmed according to the slide position. As shown in FIG. 8, the cervical columnar circumferential cells are plated at the center, the squamous columnar junction cells are plated at the far edge, and the outer cervical squamous cells are plated at the interlayers.

As described above, the cells 200 transferred onto the slide glass 500 are stained and then examined for staining, cell types, and number of nuclei, thereby diagnosing whether a donor of a sample has a specific uterine disease such as cancer. Can be used.

Although the present invention has been described with reference to the embodiments shown in the drawings, this is merely exemplary, and those skilled in the art will understand that various modifications and equivalent other embodiments are possible. Therefore, the true technical protection scope of the present invention will be defined by the technical spirit of the appended claims.

Cervical cell collection brush and brush gun according to an embodiment of the present invention can be used as a medical device for cervical cancer testing.

Claims

Uterine neck collection hair formed in the central portion on the support;

A uterine outer cervix collecting hair formed in plural on both sides of the uterine cervical collecting hair on the support; And

In the cervical cell harvesting brush comprising a connecting neck protruding toward the lower surface of the support and provided with a connecting groove at the bottom,

The cervical cervical hair collection brush is composed of at least one or more outer hair formed on the edge of the support and the inner hair having a shorter length than the outer hair formed inside the outer hair, cervical cell sampling brush.
The method of claim 1,

The uterine cervix harvesting hair is formed on both sides of the uterine cervical harvesting rod protruding in the same direction as the uterine cervical harvesting hair, cervical cell harvesting brush.
The method of claim 2,

The uterine endometrial collection hair is formed on one side of the rod for collecting the uterine cervix and the hair direction formed on the other side of the uterine cervix cell collection brush 180 degrees.
The method of claim 2,

A protective cap is attached to the end of the rod for collecting the cervix, cervical cell harvesting brush.
The method of claim 3,

The endometrial collection hair is a brush for cervical cell harvesting is perpendicular to the longitudinal direction of the support.
The method of claim 1,

The medial hair has the same length, cervical cell harvesting brush.
The method of claim 1,

The medial hair is shorter in length from the outside to the inside, cervical cell harvesting brush.
The method of claim 1,

The connecting groove is formed in a polygonal, star-shaped, straight or cross-shaped, cervical cell harvesting brush.
The method of claim 1,

A coupling groove is formed in the connecting neck, cervical cell harvesting brush.
Rotating rod having a coupling portion capable of coupling with the cervical cell collection brush of claim 1 at one end;

A trigger that is interlocked with the other end of the rotary rod to enable rotation and retraction of the rotary rod;

A main body having a handle and surrounding an interlocking portion of the rotating rod and the trigger;

It is formed on the outside of the rotating rod and includes a brush detachable part for driving the detachable by removing the cervical cell collection brush and a brush detachment switch for driving it in conjunction with the brush detachable part,

Cervical cell gun brush gun.
The method of claim 10,

The rotary rod rotates in the forward and constant directions when the trigger is pulled, and rotates in the reverse and reverse directions when the trigger is in place, cervical cell harvesting brush gun.
The method of claim 10,

Further comprising a lighting device for supplying light in a direction parallel to the longitudinal direction of the rotating rod, a power supply for supplying electricity to the lighting device and a power switch for controlling the operation of the power supply, cervical cell harvesting Dragon brush gun.
The method of claim 10,

The rotary rod and the trigger is mechanically interlocked, cervical cell harvesting brush gun.
The method of claim 10,

A cervical cell harvesting brush gun further comprising an electric motor for driving the rotating rod.
The method of claim 14,

The rotating rod and the trigger is electrically interlocked, cervical cell harvesting brush gun.