WO2013088098A3 - Efficient ultra-long dna analysis in compact nanofluidic channels - Google Patents

Efficient ultra-long dna analysis in compact nanofluidic channels Download PDF

Info

Publication number
WO2013088098A3
WO2013088098A3 PCT/GB2012/000566 GB2012000566W WO2013088098A3 WO 2013088098 A3 WO2013088098 A3 WO 2013088098A3 GB 2012000566 W GB2012000566 W GB 2012000566W WO 2013088098 A3 WO2013088098 A3 WO 2013088098A3
Authority
WO
WIPO (PCT)
Prior art keywords
compact
dna analysis
long dna
nanofluidic channels
efficient ultra
Prior art date
Application number
PCT/GB2012/000566
Other languages
French (fr)
Other versions
WO2013088098A2 (en
Inventor
Kalim Mir
Jonas Tegenfeldt
Fredrick PERSSON
Joachim FRITZSCHE
Original Assignee
Kalim Mir
Jonas Tegenfeldt
Persson Fredrick
Fritzsche Joachim
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kalim Mir, Jonas Tegenfeldt, Persson Fredrick, Fritzsche Joachim filed Critical Kalim Mir
Publication of WO2013088098A2 publication Critical patent/WO2013088098A2/en
Publication of WO2013088098A3 publication Critical patent/WO2013088098A3/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N15/1484—Electro-optical investigation, e.g. flow cytometers microstructural devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0663—Stretching or orienting elongated molecules or particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0883—Serpentine channels
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0896—Nanoscaled
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N2015/0038—Investigating nanoparticles

Abstract

The present application relates to nanochips, which can be used to visualise long molecules in a single field of view, and methods using the nanochips. These methods include mapping/barcoding and sequencing of DNA.
PCT/GB2012/000566 2011-06-30 2012-07-02 Efficient ultra-long dna analysis in compact nanofluidic channels WO2013088098A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB201111237A GB201111237D0 (en) 2011-06-30 2011-06-30 Nanochip
GB1111237.2 2011-06-30

Publications (2)

Publication Number Publication Date
WO2013088098A2 WO2013088098A2 (en) 2013-06-20
WO2013088098A3 true WO2013088098A3 (en) 2013-10-17

Family

ID=44511940

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2012/000566 WO2013088098A2 (en) 2011-06-30 2012-07-02 Efficient ultra-long dna analysis in compact nanofluidic channels

Country Status (2)

Country Link
GB (1) GB201111237D0 (en)
WO (1) WO2013088098A2 (en)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9796749B2 (en) 2011-08-05 2017-10-24 President And Fellows Of Harvard College Compositions and methods relating to nucleic acid nano- and micro-technology
WO2013039778A2 (en) 2011-09-12 2013-03-21 The University Of North Carolina At Chapel Hill Devices with a fluid transport nanochannel intersected by a fluid sensing nanochannel and related methods
WO2013119765A1 (en) 2012-02-10 2013-08-15 The University Of North Carolina At Chapel Hill Devices with fluidic nanofunnels, associated methods, fabrication and analysis systems
US10604543B2 (en) 2012-07-24 2020-03-31 President And Fellows Of Harvard College Self-assembly of nucleic acid nanostructures
US9975916B2 (en) 2012-11-06 2018-05-22 President And Fellows Of Harvard College Compositions and methods relating to complex nucleic acid nanostructures
KR20150132125A (en) 2013-02-28 2015-11-25 더 유니버시티 오브 노쓰 캐롤라이나 엣 채플 힐 Nanofluidic devices with integrated components for the controlled capture, trapping, and transport of macromolecules and related methods of analysis
NZ711033A (en) 2013-03-13 2020-03-27 Univ North Carolina Chapel Hill Nanofluidic devices for the rapid mapping of whole genomes and related systems and methods of analysis
US9765326B2 (en) 2014-04-03 2017-09-19 Stmicroelectronics S.R.L. Apparatus and method for nucleic acid sequencing based on nanochannels
US10190161B2 (en) 2014-04-03 2019-01-29 Stmicroelectronics S.R.L. Apparatus and method for nucleic acid sequencing based on nanowire detectors
JP2017523594A (en) * 2014-05-22 2017-08-17 プレジデント アンド フェローズ オブ ハーバード カレッジ Nanofabrication based on scalable nucleic acids
JP6800862B2 (en) * 2015-02-05 2020-12-16 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ Nanopore sensor including fluid passage
EP3295148A4 (en) 2015-05-11 2018-11-07 The University of North Carolina at Chapel Hill Fluidic devices with nanoscale manifolds for molecular transport, related systems and methods of analysis
CN107614675B (en) 2015-07-16 2021-08-17 香港科技大学 Dynamic formation of nanochannels for single molecule DNA analysis
US20230235379A1 (en) * 2020-04-30 2023-07-27 Dimensiongen Devices and methods for macromolecular manipulation
WO2021247394A1 (en) * 2020-06-01 2021-12-09 Dimensiongen Devices and methods for genomic analysis

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998035012A2 (en) * 1997-02-12 1998-08-13 Chan Eugene Y Methods and products for analyzing polymers
WO2001013088A1 (en) * 1999-08-13 2001-02-22 U.S. Genomics, Inc. Methods and apparatuses for stretching polymers
US20080273918A1 (en) * 2007-05-04 2008-11-06 Claros Diagnostics, Inc. Fluidic connectors and microfluidic systems
US20090115094A1 (en) * 2007-05-29 2009-05-07 Chou Stephen Y Methods for making continuous nanochannels
WO2009113010A1 (en) * 2008-03-13 2009-09-17 Nxp B.V. A sensor device and a method of detecting compounds, particles or complexes
WO2009149362A2 (en) * 2008-06-06 2009-12-10 Bionanomatrix, Inc. Integrated nanofluidic analysis devices, fabrication methods and analysis techniques
WO2010042007A1 (en) * 2008-10-10 2010-04-15 Jonas Tegenfeldt Method for the mapping of the local at/gc ratio along dna

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998035012A2 (en) * 1997-02-12 1998-08-13 Chan Eugene Y Methods and products for analyzing polymers
WO2001013088A1 (en) * 1999-08-13 2001-02-22 U.S. Genomics, Inc. Methods and apparatuses for stretching polymers
US20080273918A1 (en) * 2007-05-04 2008-11-06 Claros Diagnostics, Inc. Fluidic connectors and microfluidic systems
US20090115094A1 (en) * 2007-05-29 2009-05-07 Chou Stephen Y Methods for making continuous nanochannels
WO2009113010A1 (en) * 2008-03-13 2009-09-17 Nxp B.V. A sensor device and a method of detecting compounds, particles or complexes
WO2009149362A2 (en) * 2008-06-06 2009-12-10 Bionanomatrix, Inc. Integrated nanofluidic analysis devices, fabrication methods and analysis techniques
WO2010042007A1 (en) * 2008-10-10 2010-04-15 Jonas Tegenfeldt Method for the mapping of the local at/gc ratio along dna

Also Published As

Publication number Publication date
WO2013088098A2 (en) 2013-06-20
GB201111237D0 (en) 2011-08-17

Similar Documents

Publication Publication Date Title
WO2013088098A3 (en) Efficient ultra-long dna analysis in compact nanofluidic channels
GB2460668B (en) Subsea fluid sampling and analysis
EP2539465A4 (en) Analyte sequencing with nanopores
EP2652153A4 (en) Dna sequencing by synthesis using modified nucleotides and nanopore detection
BRPI0922952A2 (en) methods for the characterization of microorganisms in solid or semi-solid media
GB201215450D0 (en) Nanopore based device for cutting long DNA molecules into fragments
GB201014134D0 (en) Methods of RNA aplification in the presence of DNA
SG10201601638QA (en) Nucleic acid detection method, and device and kit for use in same
EP2539719A4 (en) Self-contained biological assay apparatus, methods, and applications
BRPI0807132A2 (en) enzymes for the treatment of lignocellulosics, nucleic acids encoding them, and methods and use thereof
EP2614161A4 (en) Variant annotation, analysis and selection tool
EP2352467A4 (en) Fluid collection system and methods of using same
EP2553125A4 (en) Tools and method for nanopores unzipping-dependent nucleic acid sequencing
EP2346387B8 (en) Blood analysis
EP2132334A4 (en) Methods and compositions related to nucleic acid detection
WO2011121454A3 (en) Direct nucleic acid analysis
EP2600156A4 (en) Nucleic acid analysis device
EP2318958A4 (en) Research tool access based on research session detection
GB0801479D0 (en) Improvements to methods of in field analysis
EP2208795A4 (en) Nucleic acid detection method, and nucleic acid detection kit
EP3007824A4 (en) Rna/protein/dna preferential fluid sample collection system and methods
EG26742A (en) Fluid conductivity measurement tool and methods
EP2638178A4 (en) Compositions, methods, and kits for detecting and identifying mycobacteria
EP2805281A4 (en) Methods for mapping bar-coded molecules for structural variation detection and sequencing
WO2013173583A8 (en) Psma inhibitors

Legal Events

Date Code Title Description
WWE Wipo information: entry into national phase

Ref document number: 2012842695

Country of ref document: EP

121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 12842695

Country of ref document: EP

Kind code of ref document: A2

32PN Ep: public notification in the ep bulletin as address of the adressee cannot be established

Free format text: NOTING OF LOSS OF RIGHTS PURSUANT TO RULE 112(1) EPC - FORM 1205A (26.06.2014)

122 Ep: pct application non-entry in european phase

Ref document number: 12842695

Country of ref document: EP

Kind code of ref document: A2