WO2013188305A3 - Fungal beta-xylosidase variants - Google Patents

Fungal beta-xylosidase variants Download PDF

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Publication number
WO2013188305A3
WO2013188305A3 PCT/US2013/045007 US2013045007W WO2013188305A3 WO 2013188305 A3 WO2013188305 A3 WO 2013188305A3 US 2013045007 W US2013045007 W US 2013045007W WO 2013188305 A3 WO2013188305 A3 WO 2013188305A3
Authority
WO
WIPO (PCT)
Prior art keywords
beta
xylosidase
enzyme mixtures
variants
genetically modified
Prior art date
Application number
PCT/US2013/045007
Other languages
French (fr)
Other versions
WO2013188305A2 (en
Inventor
Ryan Fong
Xiyun Zhang
Chris NORIEGA
Nicholas AGARD
Anupam Gohel
Derek Smith
Original Assignee
Codexis, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Codexis, Inc. filed Critical Codexis, Inc.
Priority to BR112014031026A priority Critical patent/BR112014031026A2/en
Priority to EP13804239.5A priority patent/EP2859099B1/en
Priority to DK13804239.5T priority patent/DK2859099T3/en
Publication of WO2013188305A2 publication Critical patent/WO2013188305A2/en
Publication of WO2013188305A3 publication Critical patent/WO2013188305A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C12N9/2482—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/14—Multiple stages of fermentation; Multiple types of microorganisms or re-use of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/16—Butanols
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01037—Xylan 1,4-beta-xylosidase (3.2.1.37)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Abstract

The present invention provides fungal xylanase and/or beta-xylosidase enzymes suitable for use in saccharification reactions. The present application further provides genetically modified fungal organisms that produce xylanase and/or beta-xylosidases, as well as enzyme mixtures exhibiting enhanced hydrolysis of cellulosic material to fermentable sugars, enzyme mixtures produced by the genetically modified fungal organisms, and methods for producing fermentable sugars from cellulose using such enzyme mixtures.
PCT/US2013/045007 2012-06-11 2013-06-10 Fungal beta-xylosidase variants WO2013188305A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
BR112014031026A BR112014031026A2 (en) 2012-06-11 2013-06-10 fungal beta-xylosidase variants.
EP13804239.5A EP2859099B1 (en) 2012-06-11 2013-06-10 Fungal beta-xylosidase variants
DK13804239.5T DK2859099T3 (en) 2012-06-11 2013-06-10 MUSHROOMS-BETA-xylosidase VERSIONS

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US201261658166P 2012-06-11 2012-06-11
US61/658,166 2012-06-11
US201261673358P 2012-07-19 2012-07-19
US61/673,358 2012-07-19
US201361774695P 2013-03-08 2013-03-08
US201361774706P 2013-03-08 2013-03-08
US61/774,706 2013-03-08
US61/774,695 2013-03-08

Publications (2)

Publication Number Publication Date
WO2013188305A2 WO2013188305A2 (en) 2013-12-19
WO2013188305A3 true WO2013188305A3 (en) 2014-04-24

Family

ID=49758847

Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/US2013/045007 WO2013188305A2 (en) 2012-06-11 2013-06-10 Fungal beta-xylosidase variants
PCT/US2013/045028 WO2013188313A2 (en) 2012-06-11 2013-06-10 Fungal xylanases and xylosidases

Family Applications After (1)

Application Number Title Priority Date Filing Date
PCT/US2013/045028 WO2013188313A2 (en) 2012-06-11 2013-06-10 Fungal xylanases and xylosidases

Country Status (5)

Country Link
US (5) US8956844B2 (en)
EP (2) EP2859096A4 (en)
BR (2) BR112014031024A2 (en)
DK (1) DK2859099T3 (en)
WO (2) WO2013188305A2 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013188305A2 (en) * 2012-06-11 2013-12-19 Codexis, Inc. Fungal beta-xylosidase variants
BR112015016329A2 (en) * 2013-01-11 2017-07-11 Codexis Inc methods for producing at least one enzyme, an enzyme mixture, fermentable sugars and at least one end product from at least one cellulosic substrate
ES2527368B1 (en) * 2013-07-22 2015-10-27 Abengoa Bioenergía Nuevas Tecnologías, S.A. Myceliophthora thermophila host cell that expresses a heterologous alpha-xylosidase enzyme and its use in a biomass degradation process
CN106778062B (en) * 2016-11-29 2019-04-02 西南大学 Aureobasidium pullulans genome dimension metabolic network model and its application
JP6547154B2 (en) * 2016-11-30 2019-07-24 本田技研工業株式会社 Communications system
TWI626312B (en) * 2017-10-25 2018-06-11 基酵生物科技股份有限公司 Xylosidase having improved enzymatic activity
CN109576245A (en) * 2018-12-27 2019-04-05 北京华美源生物科技有限公司 Barley diet barley enzyme preparation and its zymotechnique
CN111893158B (en) * 2020-08-14 2022-07-22 威海百合生物技术股份有限公司 Method for preparing cycloastragenol by double-enzyme composite conversion of astragaloside

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Also Published As

Publication number Publication date
EP2859099A4 (en) 2016-02-24
WO2013188305A2 (en) 2013-12-19
BR112014031024A2 (en) 2017-06-27
EP2859099A2 (en) 2015-04-15
WO2013188313A3 (en) 2014-04-10
US9476077B2 (en) 2016-10-25
EP2859096A2 (en) 2015-04-15
US8956844B2 (en) 2015-02-17
US20150125903A1 (en) 2015-05-07
US8980578B2 (en) 2015-03-17
US20140017733A1 (en) 2014-01-16
EP2859099B1 (en) 2019-05-08
US20140051119A1 (en) 2014-02-20
EP2859096A4 (en) 2016-05-11
DK2859099T3 (en) 2019-07-29
WO2013188313A2 (en) 2013-12-19
US20160186222A1 (en) 2016-06-30
US20150191759A1 (en) 2015-07-09
BR112014031026A2 (en) 2017-06-27

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