WO2013188313A3 - Fungal xylanases and xylosidases - Google Patents

Fungal xylanases and xylosidases Download PDF

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Publication number
WO2013188313A3
WO2013188313A3 PCT/US2013/045028 US2013045028W WO2013188313A3 WO 2013188313 A3 WO2013188313 A3 WO 2013188313A3 US 2013045028 W US2013045028 W US 2013045028W WO 2013188313 A3 WO2013188313 A3 WO 2013188313A3
Authority
WO
WIPO (PCT)
Prior art keywords
xylanase
xylosidase
enzyme mixtures
provides
enzyme
Prior art date
Application number
PCT/US2013/045028
Other languages
French (fr)
Other versions
WO2013188313A2 (en
Inventor
Nicholas J. Agard
David ELGART
Jie Yang
Goutami BANERJEE
Jeanne Bonomo Benoit
Dipnath Baidyaroy
Original Assignee
Codexis, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Codexis, Inc. filed Critical Codexis, Inc.
Priority to EP13803831.0A priority Critical patent/EP2859096A4/en
Priority to BR112014031024A priority patent/BR112014031024A2/en
Publication of WO2013188313A2 publication Critical patent/WO2013188313A2/en
Publication of WO2013188313A3 publication Critical patent/WO2013188313A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C12N9/2482—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/14—Multiple stages of fermentation; Multiple types of microorganisms or re-use of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/16—Butanols
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01037—Xylan 1,4-beta-xylosidase (3.2.1.37)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Abstract

The present invention provides fungal xylanase and/or xylosidase enzymes suitable for use in saccharification reactions. The present invention provides xylanase and xylosidase enzymes suitable for use in saccharification reactions. The present application further provides genetically modified fungal organisms that produce xylanase(s) and/or xylosidase(s), as well as enzyme mixtures exhibiting enhanced hydrolysis of cellulosic material to fermentable sugars, enzyme mixtures produced by the genetically modified fungal organisms, and methods for producing fermentable sugars from cellulose using such enzyme mixtures. In some embodiments, the xylanase and xylosidase enzyme(s) are M. thermophila enzymes.
PCT/US2013/045028 2012-06-11 2013-06-10 Fungal xylanases and xylosidases WO2013188313A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP13803831.0A EP2859096A4 (en) 2012-06-11 2013-06-10 Fungal xylanases and xylosidases
BR112014031024A BR112014031024A2 (en) 2012-06-11 2013-06-10 fungal xylanases and xylosidases.

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US201261658166P 2012-06-11 2012-06-11
US61/658,166 2012-06-11
US201261673358P 2012-07-19 2012-07-19
US61/673,358 2012-07-19
US201361774695P 2013-03-08 2013-03-08
US201361774706P 2013-03-08 2013-03-08
US61/774,695 2013-03-08
US61/774,706 2013-03-08

Publications (2)

Publication Number Publication Date
WO2013188313A2 WO2013188313A2 (en) 2013-12-19
WO2013188313A3 true WO2013188313A3 (en) 2014-04-10

Family

ID=49758847

Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/US2013/045007 WO2013188305A2 (en) 2012-06-11 2013-06-10 Fungal beta-xylosidase variants
PCT/US2013/045028 WO2013188313A2 (en) 2012-06-11 2013-06-10 Fungal xylanases and xylosidases

Family Applications Before (1)

Application Number Title Priority Date Filing Date
PCT/US2013/045007 WO2013188305A2 (en) 2012-06-11 2013-06-10 Fungal beta-xylosidase variants

Country Status (5)

Country Link
US (5) US8980578B2 (en)
EP (2) EP2859096A4 (en)
BR (2) BR112014031026A2 (en)
DK (1) DK2859099T3 (en)
WO (2) WO2013188305A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109576245A (en) * 2018-12-27 2019-04-05 北京华美源生物科技有限公司 Barley diet barley enzyme preparation and its zymotechnique

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013188305A2 (en) * 2012-06-11 2013-12-19 Codexis, Inc. Fungal beta-xylosidase variants
WO2014110223A1 (en) * 2013-01-11 2014-07-17 Codexis, Inc. Enzyme production compositions and methods
ES2527368B1 (en) * 2013-07-22 2015-10-27 Abengoa Bioenergía Nuevas Tecnologías, S.A. Myceliophthora thermophila host cell that expresses a heterologous alpha-xylosidase enzyme and its use in a biomass degradation process
CN106778062B (en) * 2016-11-29 2019-04-02 西南大学 Aureobasidium pullulans genome dimension metabolic network model and its application
JP6547154B2 (en) * 2016-11-30 2019-07-24 本田技研工業株式会社 Communications system
TWI626312B (en) * 2017-10-25 2018-06-11 基酵生物科技股份有限公司 Xylosidase having improved enzymatic activity
CN111893158B (en) * 2020-08-14 2022-07-22 威海百合生物技术股份有限公司 Method for preparing cycloastragenol by double-enzyme composite conversion of astragaloside

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110287515A1 (en) * 2009-02-06 2011-11-24 University Of Chile Protein and dna sequence encoding a cold adapted xylanase
WO2012027374A2 (en) * 2010-08-23 2012-03-01 Dyadic International (Usa) Inc. Novel fungal carbohydrate hydrolases

Family Cites Families (84)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US603344A (en) 1898-05-03 Trough for well-curbs
JPS5839517B2 (en) 1974-09-20 1983-08-30 カブシキガイシヤ バイオリサ−チセンタ− Cellulose Scala Alcohol
US3990945A (en) 1975-04-28 1976-11-09 Bio Research Center Company Limited Enzymatic hydrolysis of cellulose
DE3009104C2 (en) 1980-03-10 1990-10-04 Odenwald-Chemie GmbH, 6901 Schönau Use of an epoxy resin for the production of fire-retardant layers or coatings and processes for the production of the same
US4461648A (en) 1980-07-11 1984-07-24 Patrick Foody Method for increasing the accessibility of cellulose in lignocellulosic materials, particularly hardwoods agricultural residues and the like
US4356196A (en) 1980-10-20 1982-10-26 Hultquist Joe H Process for treating alfalfa and other cellulosic agricultural crops
US5037663A (en) 1981-10-14 1991-08-06 Colorado State University Research Foundation Process for increasing the reactivity of cellulose-containing materials
US4600590A (en) 1981-10-14 1986-07-15 Colorado State University Research Foundation Method for increasing the reactivity and digestibility of cellulose with ammonia
US4556430A (en) 1982-09-20 1985-12-03 Trustees Of Dartmouth College Process for hydrolysis of biomass
CA1338400C (en) 1983-08-31 1996-06-18 David H. Gelfand Recombinant fungal cellulases
US5171592A (en) 1990-03-02 1992-12-15 Afex Corporation Biomass refining process
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6995017B1 (en) 1994-02-17 2006-02-07 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6309883B1 (en) 1994-02-17 2001-10-30 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6395547B1 (en) 1994-02-17 2002-05-28 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6165793A (en) 1996-03-25 2000-12-26 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6406855B1 (en) 1994-02-17 2002-06-18 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6335160B1 (en) 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US20060257890A1 (en) 1996-05-20 2006-11-16 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US5834252A (en) 1995-04-18 1998-11-10 Glaxo Group Limited End-complementary polymerase reaction
US5928905A (en) 1995-04-18 1999-07-27 Glaxo Group Limited End-complementary polymerase reaction
US5837458A (en) 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
CN101659926A (en) 1994-06-30 2010-03-03 诺沃奇梅兹有限公司 Non-toxic, non-toxigenic, non-pathogenic fusarium expression system and promoters and terminators for use therein
ES2135221T3 (en) 1995-03-25 1999-10-16 Rhodia Acetow Aktiengesellscha PROCEDURE FOR THE ACTIVATION OF POLYSACCHARIDES, POLYSACCHARIDES MADE ACCORDING TO THE SAME AND THEIR USE.
FI104465B (en) 1995-06-14 2000-02-15 Valio Oy Protein hydrolyzates for the treatment and prevention of allergies and their preparation and use
US6506602B1 (en) 1996-03-25 2003-01-14 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6096548A (en) 1996-03-25 2000-08-01 Maxygen, Inc. Method for directing evolution of a virus
US7883872B2 (en) 1996-10-10 2011-02-08 Dyadic International (Usa), Inc. Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose
US5811381A (en) 1996-10-10 1998-09-22 Mark A. Emalfarb Cellulase compositions and methods of use
CA2276064C (en) 1997-01-17 2013-12-17 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
US7148054B2 (en) 1997-01-17 2006-12-12 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
US6326204B1 (en) 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
US5916780A (en) 1997-06-09 1999-06-29 Iogen Corporation Pretreatment process for conversion of cellulose to fuel ethanol
US6176176B1 (en) 1998-04-30 2001-01-23 Board Of Trustees Operating Michigan State University Apparatus for treating cellulosic materials
US6365408B1 (en) 1998-06-19 2002-04-02 Maxygen, Inc. Methods of evolving a polynucleotides by mutagenesis and recombination
DK1117808T3 (en) 1998-10-06 2005-04-25 Mark Aaron Emalfarb Transformational system before comparing the area of filamentous fungal hosts: In the chrysosporium
JP4221100B2 (en) 1999-01-13 2009-02-12 エルピーダメモリ株式会社 Semiconductor device
US6368861B1 (en) 1999-01-19 2002-04-09 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US6376246B1 (en) 1999-02-05 2002-04-23 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US6436675B1 (en) 1999-09-28 2002-08-20 Maxygen, Inc. Use of codon-varied oligonucleotide synthesis for synthetic shuffling
US6917882B2 (en) 1999-01-19 2005-07-12 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
US6961664B2 (en) 1999-01-19 2005-11-01 Maxygen Methods of populating data structures for use in evolutionary simulations
US7702464B1 (en) 2001-08-21 2010-04-20 Maxygen, Inc. Method and apparatus for codon determining
AU3210200A (en) 1999-01-19 2000-08-01 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination
US7024312B1 (en) 1999-01-19 2006-04-04 Maxygen, Inc. Methods for making character strings, polynucleotides and polypeptides having desired characteristics
US6365377B1 (en) 1999-03-05 2002-04-02 Maxygen, Inc. Recombination of insertion modified nucleic acids
US7430477B2 (en) 1999-10-12 2008-09-30 Maxygen, Inc. Methods of populating data structures for use in evolutionary simulations
US6519065B1 (en) 1999-11-05 2003-02-11 Jds Fitel Inc. Chromatic dispersion compensation device
AU4964101A (en) 2000-03-30 2001-10-15 Maxygen Inc In silico cross-over site selection
ATE327322T1 (en) 2000-09-25 2006-06-15 Iogen Energy Corp METHOD FOR PRODUCING GLUCOSE USING A MODIFIED CELLULASE
US7049485B2 (en) 2000-10-20 2006-05-23 Board Of Trustees Of Michigan State University Transgenic plants containing ligninase and cellulase which degrade lignin and cellulose to fermentable sugars
US7783428B2 (en) 2002-03-01 2010-08-24 Maxygen, Inc. Methods, systems, and software for identifying functional biomolecules
US7747391B2 (en) 2002-03-01 2010-06-29 Maxygen, Inc. Methods, systems, and software for identifying functional biomolecules
US20050084907A1 (en) 2002-03-01 2005-04-21 Maxygen, Inc. Methods, systems, and software for identifying functional biomolecules
WO2003078583A2 (en) 2002-03-09 2003-09-25 Maxygen, Inc. Optimization of crossover points for directed evolution
JP5804666B2 (en) 2005-04-12 2015-11-04 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーＥ．Ｉ．Ｄｕ Ｐｏｎｔ Ｄｅ Ｎｅｍｏｕｒｓ Ａｎｄ Ｃｏｍｐａｎｙ Concentration of separate supply streams in biomass processing and utilization
CA2610797C (en) 2005-06-03 2014-08-05 Iogen Energy Corporation Method of continuous processing of lignocellulosic feedstocks
DK2450439T3 (en) 2006-02-10 2014-02-10 Verenium Corp Cellulose-degrading enzymes, nucleic acids encoding them, and methods of preparing and utilizing them
CA2655645A1 (en) 2006-06-22 2007-12-27 Iogen Energy Corporation Enzyme compositions for the improved enzymatic hydrolysis of cellulose and methods of using same
US8017373B2 (en) 2006-08-31 2011-09-13 Iogen Energy Corporation Process for enzymatic hydrolysis of pretreated lignocellulosic feedstocks
US8680252B2 (en) 2006-12-10 2014-03-25 Dyadic International (Usa), Inc. Expression and high-throughput screening of complex expressed DNA libraries in filamentous fungi
US20080295980A1 (en) 2007-05-31 2008-12-04 Lignol Innovations Ltd. Continuous counter-current organosolv processing of lignocellulosic feedstocks
US7807419B2 (en) 2007-08-22 2010-10-05 E. I. Du Pont De Nemours And Company Process for concentrated biomass saccharification
MX2010002180A (en) 2007-08-30 2010-06-01 Iogen Energy Corp Enzymatic hydrolysis of lignocellulosic feedstocks using accessory enzymes.
WO2009033071A2 (en) 2007-09-07 2009-03-12 Dyadic International, Inc. Novel fungal enzymes
CA2726850C (en) 2008-06-13 2015-06-02 Codexis, Inc. Method of synthesizing polynucleotide variants
US8328947B2 (en) 2008-08-29 2012-12-11 Iogen Energy Corporation Method for low water hydrolysis or pretreatment of polysaccharides in a lignocellulosic feedstock
CN102421895B (en) 2009-03-16 2016-08-03 二进国际(美国)有限公司 Chrysosporium lucknowense protein production systems
WO2010120557A1 (en) 2009-03-31 2010-10-21 Codexis, Inc. Improved endoglucanases
AU2010253848C1 (en) * 2009-05-29 2015-02-19 Novozymes, Inc. Methods for enhancing the degradation or conversion of cellulosic material
CA2765715A1 (en) * 2009-06-16 2010-12-23 Codexis, Inc. .beta.-glucosidase variants
US9238792B2 (en) * 2009-09-15 2016-01-19 E I Du Pont De Nemours And Company Compartmentalized simultaneous saccharification and fermentation of biomass
DK3296394T3 (en) * 2009-09-23 2020-12-21 Danisco Us Inc STATUS UNKNOWN GLYCOSYL HYDROLASE ENZYMES AND USES
EP2504017B1 (en) 2009-11-25 2014-01-08 Codexis, Inc. Recombinant beta-glucosidase variants for production of soluble sugars from cellulosic biomass
CN103080306B (en) 2010-08-20 2015-02-18 科德克希思公司 Use of glycoside hydrolase 61 family proteins in processing of cellulose
EP2611901B1 (en) 2010-08-30 2016-05-11 Novozymes A/S Polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and polynucleotides encoding same
HUE028981T2 (en) 2010-11-02 2017-02-28 Codexis Inc Compositions and methods for production of fermentable sugars
US8476048B2 (en) * 2010-12-17 2013-07-02 E I Du Pont De Nemours And Company Xylose utilizing zymomonas mobilis with improved ethanol production in biomass hydrolysate medium
US9279163B2 (en) * 2011-08-31 2016-03-08 Iogen Energy Corporation Cellobiohydrolase enzymes
WO2013182669A2 (en) * 2012-06-08 2013-12-12 Dsm Ip Assets B.V. Novel cell wall deconstruction enzymes of myriococcum thermophilum and uses thereof
US20150175980A1 (en) * 2012-06-08 2015-06-25 Concordia University Novel cell wall deconstruction enzymes of scytalidium thermophilum, myriococcum thermophilum, and aureobasidium pullulans, and uses thereof
WO2013188305A2 (en) * 2012-06-11 2013-12-19 Codexis, Inc. Fungal beta-xylosidase variants
WO2014081700A1 (en) * 2012-11-20 2014-05-30 Codexis, Inc. Recombinant fungal polypeptides

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110287515A1 (en) * 2009-02-06 2011-11-24 University Of Chile Protein and dna sequence encoding a cold adapted xylanase
WO2012027374A2 (en) * 2010-08-23 2012-03-01 Dyadic International (Usa) Inc. Novel fungal carbohydrate hydrolases

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DATABASE UNIPROTKB/TREMBL [online] 21 March 2012 (2012-03-21), XP003034252, Database accession no. G2QKP9 *
See also references of EP2859096A4 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109576245A (en) * 2018-12-27 2019-04-05 北京华美源生物科技有限公司 Barley diet barley enzyme preparation and its zymotechnique

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US9476077B2 (en) 2016-10-25
US20150125903A1 (en) 2015-05-07
US8956844B2 (en) 2015-02-17
US20140017733A1 (en) 2014-01-16
EP2859099A2 (en) 2015-04-15
WO2013188313A2 (en) 2013-12-19
US8980578B2 (en) 2015-03-17
EP2859099B1 (en) 2019-05-08
WO2013188305A3 (en) 2014-04-24
EP2859096A2 (en) 2015-04-15
DK2859099T3 (en) 2019-07-29
US20150191759A1 (en) 2015-07-09
WO2013188305A2 (en) 2013-12-19
US20160186222A1 (en) 2016-06-30
US20140051119A1 (en) 2014-02-20
EP2859096A4 (en) 2016-05-11
BR112014031024A2 (en) 2017-06-27
EP2859099A4 (en) 2016-02-24
BR112014031026A2 (en) 2017-06-27

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