WO2014118551A1 - Electrochemical-based analytical test strip with soluble acidic material coating - Google Patents
Electrochemical-based analytical test strip with soluble acidic material coating Download PDFInfo
- Publication number
- WO2014118551A1 WO2014118551A1 PCT/GB2014/050257 GB2014050257W WO2014118551A1 WO 2014118551 A1 WO2014118551 A1 WO 2014118551A1 GB 2014050257 W GB2014050257 W GB 2014050257W WO 2014118551 A1 WO2014118551 A1 WO 2014118551A1
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- WIPO (PCT)
- Prior art keywords
- electrochemical
- sample
- test strip
- analytical test
- acidic material
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
- G01N27/3272—Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/001—Enzyme electrodes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/49—Blood
Definitions
- the present invention relates, in general, to medical devices and, in
- the determination (e.g., detection and/or concentration measurement) of an analyte in, or a characteristic of, a fluid sample is of particular interest in the medical field. For example, it can be desirable to determine glucose, ketone bodies, cholesterol, lipoproteins, triglycerides, acetaminophen, hematocrit and/or HbA1 c concentrations in a sample of a bodily fluid such as urine, blood, plasma or interstitial fluid. Such determinations can be achieved using analytical test strips, based on, for example, visual, photometric or electrochemical techniques. Conventional electrochemical-based analytical test strips are described in, for example, U.S. Patent Nos. 5,708,247, and 6,284, 125, each of which is hereby incorporated in full by reference.
- an electrochemical-based analytical test strip comprising:
- an electrically insulating base layer a patterned electrically conductive layer disposed on the electrically insulating base layer;
- an enzymatic reagent layer disposed on at least a portion of the patterned electrically conductor layer
- top layer having an underside surface
- a soluble acidic material coating on the underside surface of the top layer wherein at least the patterned spacer layer and top layer define a sample-receiving chamber within the electrochemical-based analytical test strip;
- the soluble acidic material coating is disposed on the underside surface of the top layer within at least a portion the sample-receiving chamber;
- the soluble acidic material coating is operably dissolvable in the bodily fluid sample such that a pH of the bodily fluid sample in the sample-receiving chamber is reduced during use of the electrochemical-based analytical test strip.
- the soluble acidic material coating may include a surfactant.
- the enzymatic reagent layer may include ferricyanide and the bodily fluid sample may be a whole blood sample containing uric acid.
- the soluble acidic material coating may be operably dissolvable in the bodily fluid sample such that a pH of the bodily fluid sample in the sample-receiving chamber may be reduced to a pH in the range of pH 4 to pH 6 during use of the electrochemical-based analytical test strip.
- the soluble acidic material coating may be operably dissolvable in the bodily fluid sample such that a pH of the bodily fluid sample in the sample-receiving chamber is reduced to a pH of approximately 4 during use of the
- the top layer, soluble acidic material layer may be integrated as an engineered top tape.
- the patterned electrically conductive layer may include a plurality of electrodes disposed in the sample-receiving chamber.
- the analyte may be glucose and the bodily fluid sample may be a whole blood sample.
- the soluble acidic material coating may include citric acid.
- the soluble acidic material coating may include citric acid and tri-sodium citrate.
- the citric acid and tri-sodium citrate are formulated as a pH 4 buffer.
- the soluble acidic material coating and patterned electrically conductor layer are separated by a vertical distance of approximately 100 microns in the
- a thickness of the soluble acidic material coating may be in the range of 5.8 microns to 17.3 microns.
- the soluble acidic material coating may include at least one of acetic acid, maleic acid, formic acid, and lactic acid.
- the electrically conductive layer includes at least one working electrode
- the soluble acidic material coating is disposed in the sample-receiving chamber above the at least one working electrode.
- a method for determining an analyte in a bodily fluid sample comprising: introducing a bodily fluid sample into a sample-receiving chamber of an electrochemical-based analytical test strip, the electrochemical-based analytical test strip including:
- the introduction is such that the soluble acidic material coating operably dissolves in the bodily fluid sample and reduces a pH of the bodily fluid sample in the sample-receiving chamber;
- the electrochemical-based analytical test strip may further include:
- an enzymatic reagent layer disposed on at least a portion of the patterned electrically conductor layer
- patterned spacer layer and top layer defines the sample-receiving chamber within the electrochemical-based analytical test strip.
- the detecting of an electrochemical response may involve employing a plurality of electrodes of the patterned electrically conductive layer.
- the bodily fluid sample may be a whole blood sample containing uric acid.
- the analyte may be glucose.
- the soluble acidic material coating may include a surfactant.
- the enzymatic reagent layer includes ferricyanide and the bodily fluid sample may be a whole blood sample containing uric acid.
- the soluble acidic material coating may be dissolved in the bodily fluid sample such that a pH of the bodily fluid sample in the sample-receiving chamber is reduced to a pH in the range of pH 4 to pH 6.
- the soluble acidic material coating may include citric acid.
- the soluble acidic material coating may include citric acid and tri-sodium citrate.
- the citric acid and tri-sodium citrate may be formulated as a pH 4 buffer.
- the soluble acidic material coating and patterned electrically conductor layer may be separated by a vertical distance of approximately 100 microns in the sample-receiving chamber.
- a thickness of the soluble acidic material coating may be in the range of 5.8 microns to 17.3 microns.
- the soluble acidic material coating may include at least one of acetic acid, maleic acid, formic acid, and lactic acid.
- FIG. 1 is a simplified exploded perspective view of an
- FIG. 2 is a simplified perspective view of the electrochemical-based analytical test strip of FIG. 1 ;
- FIG. 3 is a simplified cross-sectional side view of a portion of the electrochemical-based analytical test strip of FIG. 1 taken along line A-A of FIG. 2;
- FIG. 4 is a graph depicting the effect of citric acid addition on the pH of a whole blood sample
- FIGs. 5A and 5B are a graph of an electrochemical response of an electrochemical-based analytical test strip according to an embodiment of the present invention versus glucose concentration in an applied whole blood sample and a histogram of the bias of the electrochemical response in comparison to a reference measurement, respectively;
- FIGs. 6A and 6B are a graph of electrochemical response of a control electrochemical-based analytical test strip versus glucose concentration in an applied whole blood sample and a histogram of the bias of the electrochemical response in comparison to a reference measurement, respectively;
- FIG. 7 is a graph of the bias of an electrochemical-based analytical test strip according to the present invention versus uric acid concentration (i.e., level 0 of 0 mg/dL; level 1 of 5.88 mg/dL and level 2 of 1 1.75 mg /dl_);
- FIG. 8 is a graph of the bias of an electrochemical-based analytical test strip according to the present invention versus uric acid concentration (i.e., level 0 of 0 mg/dL; level 1 of 5.88 mg/dL and level 2 of 1 1 .75 mg /dL); and
- FIG. 9 is a flow diagram depicting stages in a method for determining an analyte in a bodily fluid sample according to an embodiment of the present invention.
- an analyte such as glucose
- a bodily fluid sample for example, a whole blood sample
- an analyte such as glucose
- a bodily fluid sample for example, a whole blood sample
- an analyte such as glucose
- a bodily fluid sample for example, a whole blood sample
- an analyte such as glucose
- a bodily fluid sample for example, a whole blood sample
- an analyte such as glucose
- a bodily fluid sample for example, a whole blood sample
- the determination of an analyte in a bodily fluid sample can be susceptible to determination inaccuracies arising from the presence of endogenous and exogenous substances in the blood sample (referred to as interferent compounds or simply "interferents").
- interferent compounds can give rise to measurement inaccuracies through two mechanisms. Firstly, the interferent compound may be directly oxidized at an electrode surface, giving rise to a direct interference error current. Secondly, the interferent compound may react with a mediator of the enzymatic reagent, giving rise to an indirect interference error current.
- Uric acid in whole blood samples is one such interferent and can be present at endogenous levels in the range of, for example, 3 mg/dL to 8 mg/dL.
- Electrochemical-based analytical test strips according to embodiments of the present invention are beneficial in that, for example, the reduced pH of the bodily fluid sample can serve to reduce the deleterious effect of interferents (such as uric acid in a whole blood sample) on an electrochemical response of the analytical test strip that is employed in the determination. Moreover, the soluble acidic material coating does not increase the volume of the bodily fluid sample.
- the soluble acidic material coating is disposed on the underside surface of the top layer, (i) the soluble acidic material is not in contact with the enzymatic reagent layer, thus preventing any deleterious impact on the enzymatic reagent layer such as, for example, enzyme denaturating, and (ii) during use, the pH of the bodily fluid sample is lowered without exposing the enzymatic reagent layer or dissolved components thereof to an overly aggressive environment.
- embodiments of the present invention are particularly beneficial in regards to the interferent uric acid in a whole blood sample in combination with an enzymatic reagent layer that includes ferricyanide.
- a reduced pH results in less of the uric acid being speciated in an electrochemically active monoanion form and also lessened indirect interference between uric acid and ferricyanide.
- Similar benefits are expected for any interferents for which the mechanism of interference is similar to that of uric acid. Specifically, for those interferents that are speciated at low pH in a manner that is less electrochemically active than at physiological pH and / or less reactive towards an enzymatic reagent layer mediator at low pH than at physiological pH.
- FIG. 1 is a simplified exploded perspective view of an
- FIG. 2 is a simplified perspective view of
- FIG. 3 is a simplified
- FIG. 4 is a graph depicting the effect of citric acid addition on the pH of a whole blood sample.
- electrochemical-based analytical test strip 100 for the determination of an analyte (such as glucose) in a bodily fluid sample includes an electrically-insulating base layer 1 10, a patterned electrically conductive layer 120, an optional patterned insulation layer 130, enzymatic reagent layer 140, a patterned spacer layer 150, a soluble acidic material coating 160, a top layer 170 consisting of a hydrophilic sub-layer 172 and a top tape 174. Hydrophilic sub-layer 172 of top layer 170 has an underside surface 176 (see FIG. 3 in particular).
- sample-receiving chamber 180 within electrochemical-based analytical test strip 100 (see FIG. 3 in particular where the introduction of a bodily fluid sample (i.e., blood) into sample-receiving chamber 180 is depicted with an arrow).
- a bodily fluid sample i.e., blood
- Electrically-insulating base layer 1 10 can be any suitable material
- the electrically-insulating base layer can have any suitable dimensions including, for example, a width dimension of about 5 mm, a length dimension of about 27 mm and a thickness dimension of about 0.5 mm.
- Electrically-insulating base layer 1 10 provides structure to
- electrochemical-based analytical test strip 100 for ease of handling and also serves as a base for the application (e.g., printing or deposition) of subsequent layers (e.g., a patterned electrically conductor layer).
- Patterned electrically conductive layer 120 is disposed on the electrically- insulating base layer 1 10 and includes a first electrode 122, a second electrode 124 and a third electrode 126.
- First electrode 122, second electrode 124 and third electrode 126 can be, for example, configured as a counter/reference electrode, working electrode and another working electrode, respectively.
- electrochemical-based analytical test strip 100 is depicted as including a total of three electrodes, embodiments of electrochemical-based analytical test strips, including embodiments of the present invention, can include any suitable number of electrodes.
- Patterned electrically conductive layer 120, including first electrode 122, second electrode 124 and third electrode 126, of electrochemical-based analytical test strip 100 can be formed of any suitable conductive material including, for example, gold, palladium, platinum, indium, titanium-palladium alloys and electrically conducting carbon-based materials including carbon inks. It should be noted that patterned electrically conductor layers employed in analytical test strips according to embodiments of the present invention can take any suitable shape and be formed of any suitable materials including, for example, metal materials and conductive carbon materials.
- electrochemical-based analytical test strip 100 is configured for the electrochemical determination of an analyte (such as glucose) in a bodily fluid sample (such as a whole blood sample containing the interferent uric acid) that has filled sample-receiving chamber 180.
- an analyte such as glucose
- a bodily fluid sample such as a whole blood sample containing the interferent uric acid
- Enzymatic reagent layer 140 is disposed on at least a portion of patterned electrically conductor layer 120.
- Enzymatic reagent layer 140 can include any suitable enzymatic reagents, with the selection of enzymatic reagents being dependent on the analyte to be determined. For example, if glucose is to be determined in a blood sample, enzymatic reagent layer 140 can include a glucose oxidase or glucose dehydrogenase along with other components necessary for functional operation.
- Enzymatic reagent layer 140 can include, for example, glucose oxidase, tri-sodium citrate, citric acid, polyvinyl alcohol, hydroxyl ethyl cellulose, potassium ferricyanide, potassium ferrocyanide, antifoam, fumed silica (either with or without a hydrophobic surface modification), PVPVA, and water. Further details regarding reagent layers, and electrochemical-based analytical test strips in general, are in U.S. Patent Nos. 6,241 ,862 and 6,733,655, the contents of which are hereby fully incorporated by reference.
- the amount of acidic material employed in enzymatic reagents is not sufficient to reduce the pH of a bodily fluid sample to the levels required to provide beneficially reduced interferent effects.
- Patterned spacer layer 150 can be formed, for example, from a
- patterned spacer layer 150 defines outer walls of the sample-receiving chamber 180.
- Patterned spacer layer 150 can have a thickness of, for example, approximately 75 microns, be electrically nonconductive, and be formed of a polyester material with top and bottom side acrylic-based pressure sensitive adhesive.
- Soluble acidic material coating 160 is disposed on the underside surface
- soluble acidic material coating 160 is operably dissolvable in the bodily fluid sample such that a pH of the bodily fluid sample in the sample-receiving chamber is reduced during use of the electrochemical-based analytical test strip.
- CA citric acid
- a pH of 4 is sufficient to substantially protonate the interferent uric acid in whole blood, rendering the uric acid electrochemically inactive, thus effectively eliminating any interfering reaction between uric acid and ferricyanide that is commonly present in an enzymatic reagent layer.
- the electrochemically inactive form of uric acid does not oxidize at an electrode surface.
- the reaction rate between ferricyanide and uric acid is significantly reduced, the amount of ferrocyanide generated by such a reaction and oxidized at the electrode surface is also significantly reduced.
- Such an addition of citric acid would, therefore, be expected to result in a substantial reduction in the interfering effect of uric acid on the determination of glucose in a whole blood sample that contains uric acid.
- the amount of soluble acidic material coated on the top layer can beneficially be, for example, sufficient to reduce the bodily fluid sample pH into the range of pH 4 to pH 6.
- the interfering effect of the uric acid is substantially reduced compared to that at physiological pH due to a reduction in both the concentration of the electrochemically active monoanion of uric acid and the reaction rate between potassium ferricyanide and uric acid to form ferrocyanide.
- the amount of soluble acidic material in the soluble acidic material coating can be such that the bodily fluid sample pH in the region of the enzymatic reagent layer is not reduced to pH 3, at which point the combination of low pH and presence of ferricyanide can result in the deleterious de-activation of glucose oxidase.
- citric acid was selected as the acidic material for the soluble acidic material coating.
- any suitable acidic material can be employed in embodiments of the present invention as long as it is readily soluble in the bodily fluid sample, diffuses rapidly and does not have any detrimental effect on the enzymatic reagent chemistry.
- other weak acids such as acetic acid, maleic acid, formic acid or lactic acid could be suitable depending on the analyte, bodily fluid sample and enzymatic reagent layer characteristics.
- COMSOL a commercially available finite element modeling software package
- modeling indicates that dissolution and diffusion into the bodily fluid sample of citric acid based soluble acidic material coatings with thicknesses in the range of 5.8 microns to 17.3 microns are effective in beneficially reducing the pH of a whole blood sample.
- the pH was reduced to below pH 6 throughout a sample chamber below pH within 2 seconds of bodily fluid sample introduction.
- the pH throughout the sample chamber was in the range of pH 3.5 to pH 4.5, sufficiently low to effect a reduction in both the concentration of the electrochemically active monoanion of uric acid and the reaction rate between potassium ferricyanide and uric acid to form ferrocyanide, thus reducing the interfering effect of uric acid.
- the pH local to the electrode's surfaces was greater than pH 4, hence no or minimal deactivation of enzyme within the enzymatic reagent layer would be predicted and the glucose response is expected to be unimpaired.
- the dissolution of a 17.3 pm thickness soluble acidic material coating would lower the pH throughout the sample chamber sufficiently to effect a reduction in uric acid interference, without deactivating the enzyme to the extent that the glucose sensitivity of the electrochemical-based analytical test strip is compromised.
- Top layer 170 can be, for example, a clear film with hydrophilic properties that promote wetting and filling of electrochemical-based analytical test strip 100 by a fluid sample (e.g., a whole blood sample).
- a fluid sample e.g., a whole blood sample.
- Top layer 170 can be, for example, a polyester film coated with a surfactant that provides a hydrophilic contact angle ⁇ 10 degrees.
- Top layer 170 can also be a polypropylene film coated with a surfactant or other surface treatment. In such a circumstance, the surfactant coating serves as hydrophilic sub-layer 172.
- the soluble acidic material coating can be formulated as a hydrophilic coating and also serve as a hydrophilic sub-layer.
- Top layer 170 can have a thickness, for example, of approximately 100pm.
- Electrochemical-based analytical test strip 100 can be manufactured, for example, by the sequential aligned formation of patterned electrically conductor layer 120, enzymatic reagent layer 140, patterned spacer layer 150, and hydrophilic sub-layer 172 onto electrically-insulating base layer 1 10. Any suitable techniques known to one skilled in the art can be used to accomplish such sequential aligned formation, including, for example, screen printing, photolithography, photogravure, chemical vapour deposition and tape lamination techniques.
- FIGs. 5A and 5B are a graph of an electrochemical response from an electrochemical-based analytical test strip according to the present invention versus glucose concentration in an applied whole blood sample, and a histogram of the bias of the electrochemical response in comparison to a reference measurement, respectively.
- FIGs. 6A and 6B are a graph of electrochemical response from a control electrochemical-based analytical test strip versus glucose concentration in an applied whole blood sample and a histogram of the bias of the electrochemical response in comparison to a reference
- FIG. 7 is a graph of the bias of an electrochemical-based analytical test strip according to the present invention versus uric acid
- FIG. 8 is a graph of the bias of an electrochemical-based analytical test strip according to the present invention versus uric acid concentration (i.e., level 0 of 0 mg/dL; level 1 of 5.88 mg/dL and level 2 of 1 1 .75 mg /dL).
- electrochemical-based analytical test strip according to the present invention were manufactured using citric acid (700 g/L) and tri-sodium citrate (400g/L) mixed together to generate a concentrated buffer solution with pH 4.
- Tergitol NP7 a surfactant
- was added at 0.5%) to increase the wettability of the soluble acidic material coating, and to ensure near-instantaneous dissolution thereof in a bodily fluid sample. It was determined that such near-instantaneous and uniform dissolution resulted in a precision improvement in the case of the formulation with added surfactant when compared to the case without any added surfactant. Further investigations revealed that surfactant concentrations up to 5% can be beneficial in terms of increasing the precision of analyte determination.
- the acidic solution was then spray coated onto the underside of a top layer using a Biodot AD3050 spray apparatus at a dispense rate of 1 .7 micro-liter per square-cm. Such as dispense rate was calculated, using the bulk densities of citric acid and trisodium citrate, to provide a dried soluble acidic material coating with a thickness of 17.3 pm.
- the acid-coated top layer thus prepared was then used to manufacture electrochemical-based analytical test strips using standard procedures.
- FIG. 9 is a flow diagram depicting stages in a method 900 for determining an analyte (such as glucose) in a bodily fluid sample (for example, a whole blood sample) according to an embodiment of the present invention.
- Method 900 includes, at step 910, introducing a bodily fluid sample into a sample-receiving chamber of an electrochemical-based analytical test strip with the
- electrochemical-based analytical test strip including a top layer with an underside surface and a soluble acidic material coating on the underside surface within at least a portion the sample-receiving chamber.
- the introduction of step 910 is such that the soluble acidic material coating operably dissolves in the bodily fluid sample and reduces a pH of the bodily fluid sample in the
- electrochemical-based analytical test strip is detected.
- an analyte in the bodily fluid sample is determined based on the detected analytical response.
- method 900 can be readily modified to incorporate any of the techniques, benefits, features and characteristics of electrochemical-based analytical test strips according to embodiments of the present invention and described herein. While preferred embodiments of the present invention have been shown and described herein, it will be obvious to those skilled in the art that such embodiments are provided by way of example only. Numerous variations, changes, and substitutions will now occur to those skilled in the art without departing from the invention. It should be understood that various alternatives to the embodiments of the invention described herein may be employed in practicing the invention. It is intended that the following claims define the scope of the invention and that devices and methods within the scope of these claims and their equivalents be covered thereby.
Abstract
Description
Claims
Priority Applications (9)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CA2899372A CA2899372A1 (en) | 2013-01-31 | 2014-01-30 | Electrochemical-based analytical test strip with soluble acidic material coating |
RU2015136508A RU2015136508A (en) | 2013-01-31 | 2014-01-30 | ELECTROCHEMICAL ANALYTICAL TEST STRIP COATED WITH SOLUBLE ACID MATERIAL |
US14/764,427 US20150362453A1 (en) | 2013-01-31 | 2014-01-30 | Electrochemical-based analytical test strip with soluble acidic material coating |
BR112015017894A BR112015017894A2 (en) | 2013-01-31 | 2014-01-30 | electrochemical based analytical test strip with soluble acid material coating |
JP2015555799A JP2016505153A (en) | 2013-01-31 | 2014-01-30 | Electrochemical analytical test strip with soluble acidic material coating |
KR1020157022926A KR20150111981A (en) | 2013-01-31 | 2014-01-30 | Electrochemical-based analytical test strip with soluble acidic material coating |
CN201480007018.2A CN104968798A (en) | 2013-01-31 | 2014-01-30 | Electrochemical-based analytical test strip with soluble acidic material coating |
AU2014210961A AU2014210961A1 (en) | 2013-01-31 | 2014-01-30 | Electrochemical-based analytical test strip with soluble acidic material coating |
EP14702927.6A EP2951312A1 (en) | 2013-01-31 | 2014-01-30 | Electrochemical-based analytical test strip with soluble acidic material coating |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB1301747.0 | 2013-01-31 | ||
GB1301747.0A GB2510371B (en) | 2013-01-31 | 2013-01-31 | Electrochemical-based analytical test strip with soluble acidic material coating |
Publications (1)
Publication Number | Publication Date |
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WO2014118551A1 true WO2014118551A1 (en) | 2014-08-07 |
Family
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PCT/GB2014/050257 WO2014118551A1 (en) | 2013-01-31 | 2014-01-30 | Electrochemical-based analytical test strip with soluble acidic material coating |
Country Status (13)
Country | Link |
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US (1) | US20150362453A1 (en) |
EP (1) | EP2951312A1 (en) |
JP (1) | JP2016505153A (en) |
KR (1) | KR20150111981A (en) |
CN (1) | CN104968798A (en) |
AU (1) | AU2014210961A1 (en) |
BR (1) | BR112015017894A2 (en) |
CA (1) | CA2899372A1 (en) |
GB (1) | GB2510371B (en) |
HK (1) | HK1200537A1 (en) |
RU (1) | RU2015136508A (en) |
TW (1) | TW201432258A (en) |
WO (1) | WO2014118551A1 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2017040712A1 (en) | 2015-09-01 | 2017-03-09 | Polymer Technology Systems, Inc. | Systems and methods for blood sample preservation and hematocrit separation |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
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EP3397954B1 (en) * | 2015-12-28 | 2024-04-03 | Lifescan Scotland Limited | Hand-held test meter for use with electrochemical-based analytical test strip with electrode voltage sensing connections |
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US5708247A (en) * | 1996-02-14 | 1998-01-13 | Selfcare, Inc. | Disposable glucose test strips, and methods and compositions for making same |
US6241862B1 (en) * | 1996-02-14 | 2001-06-05 | Inverness Medical Technology, Inc. | Disposable test strips with integrated reagent/blood separation layer |
EP1398626A1 (en) * | 2001-06-14 | 2004-03-17 | Matsushita Electric Industrial Co., Ltd. | Biosensor |
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JPH0795056B2 (en) * | 1987-10-29 | 1995-10-11 | 松下電器産業株式会社 | Biosensor |
JPH06138080A (en) * | 1992-03-12 | 1994-05-20 | Matsushita Electric Ind Co Ltd | Biosensor |
JPH08136495A (en) * | 1994-11-08 | 1996-05-31 | Matsushita Electric Ind Co Ltd | Biosensor |
JPH08304328A (en) * | 1995-05-08 | 1996-11-22 | Matsushita Electric Ind Co Ltd | Biosensor |
AUPN363995A0 (en) * | 1995-06-19 | 1995-07-13 | Memtec Limited | Electrochemical cell |
CN1846131B (en) * | 2003-06-20 | 2012-01-18 | 霍夫曼-拉罗奇有限公司 | Method and reagent for producing narrow, homogenous reagent strips |
US8364230B2 (en) * | 2006-10-04 | 2013-01-29 | Dexcom, Inc. | Analyte sensor |
CN2748912Y (en) * | 2004-09-07 | 2005-12-28 | 桂林工学院 | Disposable electrode type blood sugar test bar |
EP2084292B1 (en) * | 2006-10-05 | 2011-02-23 | Lifescan Scotland Limited | A reagent formulation using ruthenium hexamine as a mediator for electrochemical test strips |
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2013
- 2013-01-31 GB GB1301747.0A patent/GB2510371B/en not_active Expired - Fee Related
- 2013-10-29 TW TW102139000A patent/TW201432258A/en unknown
-
2014
- 2014-01-30 CN CN201480007018.2A patent/CN104968798A/en active Pending
- 2014-01-30 AU AU2014210961A patent/AU2014210961A1/en not_active Abandoned
- 2014-01-30 US US14/764,427 patent/US20150362453A1/en not_active Abandoned
- 2014-01-30 CA CA2899372A patent/CA2899372A1/en not_active Abandoned
- 2014-01-30 WO PCT/GB2014/050257 patent/WO2014118551A1/en active Application Filing
- 2014-01-30 JP JP2015555799A patent/JP2016505153A/en active Pending
- 2014-01-30 EP EP14702927.6A patent/EP2951312A1/en not_active Withdrawn
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Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2017040712A1 (en) | 2015-09-01 | 2017-03-09 | Polymer Technology Systems, Inc. | Systems and methods for blood sample preservation and hematocrit separation |
CN108291907A (en) * | 2015-09-01 | 2018-07-17 | 聚合物工艺系统有限公司 | The system and method detached with hematocrit value is preserved for blood sample |
US10145840B2 (en) | 2015-09-01 | 2018-12-04 | Polymer Technology Systems, Inc. | Systems and methods for blood sample preservation and hematocrit separation |
EP3344998A4 (en) * | 2015-09-01 | 2019-04-17 | Polymer Technology Systems, Inc. | Systems and methods for blood sample preservation and hematocrit separation |
CN108291907B (en) * | 2015-09-01 | 2021-06-11 | 聚合物工艺系统有限公司 | Systems and methods for blood sample preservation and hematocrit separation |
Also Published As
Publication number | Publication date |
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AU2014210961A1 (en) | 2015-09-17 |
CN104968798A (en) | 2015-10-07 |
BR112015017894A2 (en) | 2017-07-11 |
HK1200537A1 (en) | 2015-08-07 |
EP2951312A1 (en) | 2015-12-09 |
GB2510371A (en) | 2014-08-06 |
GB201301747D0 (en) | 2013-03-20 |
RU2015136508A (en) | 2017-03-07 |
KR20150111981A (en) | 2015-10-06 |
US20150362453A1 (en) | 2015-12-17 |
CA2899372A1 (en) | 2014-08-07 |
GB2510371B (en) | 2016-01-06 |
TW201432258A (en) | 2014-08-16 |
JP2016505153A (en) | 2016-02-18 |
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