WO2015080175A1 - Acidic protein beverage and method for manufacturing same - Google Patents

Acidic protein beverage and method for manufacturing same Download PDF

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Publication number
WO2015080175A1
WO2015080175A1 PCT/JP2014/081303 JP2014081303W WO2015080175A1 WO 2015080175 A1 WO2015080175 A1 WO 2015080175A1 JP 2014081303 W JP2014081303 W JP 2014081303W WO 2015080175 A1 WO2015080175 A1 WO 2015080175A1
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beverage
weight
acidic protein
water
gum arabic
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PCT/JP2014/081303
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French (fr)
Japanese (ja)
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悠悟 島
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不二製油株式会社
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Publication of WO2015080175A1 publication Critical patent/WO2015080175A1/en

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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52Adding ingredients
    • A23L2/66Proteins
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52Adding ingredients

Definitions

  • the present invention relates to an acidic protein beverage and a method for producing the same.
  • Patent Document 5 describes that a beverage having high stability and having no pasty feeling can be prepared by using pectin and gum arabic in combination. However, even in the technique of Patent Document 5, the viscosity of the beverage is high, it is difficult to say that it is a refreshing mouth, and the stability is insufficient, and further improvement is desired.
  • the present inventors have added water-soluble soy polysaccharide and gum arabic, which has a low viscosity and suppresses the aggregation and precipitation of proteins.
  • the present inventors have found that an acidic protein beverage with good dispersion stability can be provided.
  • the present invention (1) an acidic protein beverage having a pH of 3.3 to 4.4 containing a water-soluble soybean polysaccharide and gum arabic; (2) The acidic protein beverage according to (1), containing 0.01 to 2% by weight of protein, (3) The acidic protein beverage according to (1) or (2), containing 0.01 to 1% by weight of water-soluble soybean polysaccharide and 0.05 to 1% by weight of gum arabic with respect to the beverage, (4) The viscosity of the acidic protein beverage is a B-type rotational viscometer.
  • the present invention it is possible to provide a beverage having a good dispersion stability in which protein aggregation and precipitation are significantly suppressed as compared with conventional ones with a clean mouth with low viscosity.
  • the acidic protein beverage of the present invention is characterized by containing a water-soluble soybean polysaccharide and gum arabic.
  • the acidic protein beverage in the present invention is an addition of an organic acid such as lactic acid or citric acid to a protein-containing solution such as milk, whole milk powder, sweetened condensed milk, skimmed powdered milk, sweetened defatted condensed milk, defatted concentrated milk, soy milk, soy protein aqueous solution, etc.
  • a directly acidified beverage acidified by fermentation a fermented milk beverage such as a drink yogurt or a lactic acid bacteria beverage prepared by fermenting the above-described protein-containing solution with lactic acid bacteria, and frozen yogurt obtained by freezing them.
  • Examples of acidic conditions include those having a pH of 3.3 to 4.4.
  • the pH is 3.2 or lower, the protein used in the beverage is almost solubilized, so that the water-soluble soybean polysaccharide and gum arabic are no longer necessary.
  • the effect is seen even at pH 4.5 or more, but since the addition amount of water-soluble soybean polysaccharide and gum arabic is very large, the beverage production cost is increased and the viscosity is increased.
  • the protein content in the acidic protein beverage that can be stabilized without increasing the viscosity of the beverage is preferably 2% by weight or less, more preferably 1.5% by weight or less, and even more preferably 1% by weight or less. .
  • 0.05 weight% or more is more preferable. Stabilization is possible even with a protein content higher than 2% by weight, but the amount of water-soluble soybean polysaccharide and gum arabic required for stabilization increases, which may increase costs and viscosity of the beverage. It may not be preferable in preparing a refreshing beverage that is the object of the present invention. Further, if it is less than 0.01% by weight, the problem of aggregation may hardly occur, and the addition of water-soluble soybean polysaccharide and gum arabic may be unnecessary.
  • the viscosity is important in order to make the beverage a refreshing drink, and using a B-type rotational viscometer (manufactured by Toki Sangyo Co., Ltd.), the rotor No.
  • the viscosity when measured under the conditions of 19, 60 rpm, 1 minute, and 20 ° C. is preferably 5 mPa ⁇ s or less, more preferably 3 mPa ⁇ s or less, and even more preferably 2 mPa ⁇ s or less.
  • the acidic protein beverage containing the water-soluble soybean polysaccharide and gum arabic of the present invention has a low-viscosity, clean drinking mouth under the acidity of pH 3.3 to 4.4, and more protein aggregation and precipitation than before. It is greatly suppressed and the dispersion stability becomes better.
  • water-soluble soybean polysaccharide or gum arabic is used alone, the target quality cannot be obtained in terms of dispersion stability.
  • the water-soluble soybean polysaccharide in the present invention can be obtained by various methods, for example, by the method described in Japanese Patent No. 2599477. If an example of a manufacturing method is shown, 2 times weight part water will be added to the raw okara obtained by the manufacturing process of isolation
  • the polysaccharide solution is precipitated with 60% by weight of ethanol at the end, the recovered precipitate is washed with 90% by weight of water-containing ethanol, and the resulting precipitate is air-dried to obtain a water-soluble soybean polysaccharide.
  • the amount of water-soluble soybean polysaccharide added depends on the protein content in the beverage, but is preferably 0.01 to 1% by weight, more preferably 0.02 to 0.7% by weight, and still more preferably based on the beverage. Is 0.1 to 0.4% by weight. If the amount is less than 0.01% by weight, the protein dispersion stabilizing effect may be insufficient. Conversely, if the amount exceeds 1% by weight, the production cost increases, which may not be preferable.
  • the gum arabic used in the present invention can be used regardless of the origin species such as Acacia senegal and Acacia sial. Such gum arabic is commercially available. Examples thereof include “Gum Arabic SD” manufactured by San-Ei Gen FFI Co., Ltd. and “Tikacia Gum SD-RN” manufactured by TIC GUM.
  • the amount of gum arabic added depends on the protein content in the beverage and the amount of water-soluble soybean polysaccharide to be added, but is preferably 0.05 to 1% by weight, more preferably 0.1 to 0%, based on the beverage. 0.7% by weight, still more preferably 0.2-0.5% by weight. If the amount is less than 0.05% by weight, the protein dispersion stabilizing effect may be insufficient. On the other hand, if it exceeds 1% by weight, the production cost increases, which may be undesirable.
  • the acidic protein beverage of the present invention it is essential to mix water-soluble soybean polysaccharide and gum arabic, but other materials may be used in combination as long as the effects of the present invention are not adversely affected.
  • Other materials include agar, carrageenan, farseleran, tamarind seed polysaccharide, tara gum, karaya gum, pectin, xanthan gum, sodium alginate, tragacanth gum, guar gum, locust bean gum, pullulan, gellan gum, carboxymethylcellulose (CMC), alginic acid as stabilizers.
  • CMC carboxymethylcellulose
  • Various polysaccharides such as propylene glycol ester and processed starch are listed.
  • Food ingredients include sucrose, sugar, fructose, glucose liquid sugar, dextrin, maltose, trehalose, sorbitol, xylitol, maltitol and other sugars and sugar alcohols
  • food additives include glycerin fatty acid esters and sucrose fatty acids. Examples include emulsifiers such as esters, salts such as calcium fortifiers, organic acid salts such as sodium citrate and sodium acetate, preservatives such as benzoic acid, sorbic acid, shirako protein, and glycine.
  • the method for preparing the acidic protein beverage of the present invention is not particularly limited as long as it is prepared so as to contain a water-soluble soybean polysaccharide and gum arabic.
  • a protein-containing solution, a water-soluble soybean polysaccharide and gum arabic are prepared.
  • the pH of the mixture is adjusted to 3.3 to 4.4 with an organic acid such as lactic acid or citric acid, and manufactured through a homogenization process, a heat sterilization process, and a container filling process.
  • Examples include a method of preparing and mixing and preparing a mixed solution through a homogenization step, a heat sterilization step, and a container filling step.
  • An acidic protein beverage was prepared by the method described below, and the pH, viscosity, precipitation rate, and particle size distribution (median diameter) of the beverage after preparation and storage at 5 ° C. for 1 week were evaluated.
  • ⁇ pH The measurement was performed at 20 ° C. with pH METER F-71 (manufactured by Horiba, Ltd.).
  • ⁇ Viscosity Using a B-type rotational viscometer (manufactured by Toki Sangyo Co., Ltd.), rotor No. The measurement was performed at 19, 60 rpm, 1 minute, and 20 ° C.
  • Precipitation rate (precipitation weight / sample weight) ⁇ 100.
  • the prepared beverage has a viscosity of 5 mPa ⁇ s or less, a particle size distribution (median diameter) of 1.2 ⁇ m or less, and a precipitation rate of 1.2% or less as a quality that has low viscosity and excellent beverage stability. did.
  • Example 1 Preparation of Sterilized Acidic Protein Beverage Containing 1.0% Protein After mixing the raw material liquids according to the formulation shown in Table 1, adjusting the pH to 4.0 with a 50% citric acid solution, the total amount is 100% with water. And homogenization was performed at 150 kg / cm 2 . The homogenized solution was sterilized at 90 ° C. for 20 minutes, filled into a container, and then cooled to obtain an acidic protein beverage containing 1% protein.
  • Example 1 A beverage was prepared in the same manner as in Example 1 except that 5% ticasia gum solution was used and only Soya Five-S was used as a stabilizer.
  • Example 2 A beverage was prepared in the same manner as in Example 1, except that 5% Soyafive-S solution was used and only ticasia gum was used as a stabilizer.
  • Example 2 The raw material liquids were mixed according to the formulation shown in Table 2, and the pH was adjusted to 4.4 with a 50% citric acid solution. The total amount was adjusted to 100% with water and homogenized at 150 kg / cm 2 . The homogenized solution was sterilized at 90 ° C. for 20 minutes, filled into a container, and then cooled to obtain a beverage.
  • Example 3 a beverage was prepared in exactly the same manner except that 5% ticasia gum solution was used and only Soyafive-S was used as a stabilizer.
  • Example 4 a beverage was prepared in exactly the same manner except that 5% Soyafive-S solution was used and only ticasia gum was used as a stabilizer.
  • Example 3 Preparation of Sterilized Acidic Protein Beverage Containing 0.3% Protein After mixing raw material liquids according to the formulation shown in Table 3 and adjusting the pH to 4.2 with 50% citric acid solution, the total amount is 100% with water. And homogenization was performed at 150 kg / cm 2 . The homogenized solution was sterilized at 90 ° C. for 20 minutes, filled into a container and then cooled to obtain an acidic protein beverage containing 0.3% protein.
  • Example 5 A beverage was prepared in exactly the same manner as in Example 3 except that 5% ticasia gum solution was used and only Soya Five-S was used as a stabilizer.
  • Example 6 (Comparative Example 6) In Example 3, a beverage was prepared in exactly the same manner except that 5% Soyafive-S solution was used and only ticasia gum was used as a stabilizer.
  • Glucose 5% was added to skim milk powder 15%, and the total amount was adjusted to 100% with water, followed by sterilization at 90 ° C. for 30 minutes to prepare a 15% skim milk powder solution.
  • L. Casei was added at 2.5 ppm, fermented at 38 ° C. for 120 hours, and the resulting fermentation broth was crushed with a mixer, ice-cooled to 5 ° C., and then homogenized at 150 kg / cm 2 to obtain fermented milk. It was.
  • the pH of the obtained fermented milk was 3.6.
  • the obtained fermented milk was stored at 5 degreeC.
  • Example 4 raw material liquid mixed in accordance with the formulation of the preparation Table 4 Protein 1.0% content lactobacillus beverage, the total amount of water as 100%, was subjected to homogenization at 150 kg / cm 2. After filling the homogenized solution into a container, a lactic acid bacteria beverage containing 1.0% protein was obtained.
  • Example 5 A beverage was prepared in the same manner as in Example 4 except that the addition amount of the 5% ticasia gum solution was reduced from 6.0% to 2.0%.
  • Example 7 A beverage was prepared in the same manner as in Example 4 except that 5% ticasia gum solution was used and only Soya Five-S was used as a stabilizer.
  • Example 8 In Example 4, a beverage was prepared in exactly the same manner except that 5% Soyafive-S solution was used and only ticasia gum was used as a stabilizer.
  • Example 6 Preparation of 0.3% Protein-Containing Lactic Acid Beverage Beverages The raw material liquids were mixed according to the formulation shown in Table 5, and the total amount was made 100% with water, and homogenized at 150 kg / cm 2 . After filling the homogenized solution into a container, a lactic acid bacteria beverage containing 0.3% protein was obtained.
  • Example 6 (Comparative Example 9) In Example 6, a beverage was prepared in exactly the same manner except that 5% ticasia gum solution was used and only Soya Five-S was used as a stabilizer.
  • Example 10 A beverage was prepared in the same manner as in Example 6 except that 5% Soyafive-S solution was used and only ticasia gum was used as a stabilizer.
  • the use of water-soluble soybean polysaccharide and gum arabic for acidic protein beverages can effectively improve the stability of beverages, and can be seen when other stabilizers are used in combination. It has become possible to provide a refreshing beverage with a low viscosity without increasing the viscosity of the beverage.

Abstract

[Problem] The present invention addresses the problem of providing a low-viscosity acidic protein beverage in which the capability of dispersing and stabilizing the protein is improved over the past, the beverage having a mild and refreshing flavor. [Solution] It is possible to provide an acidic protein beverage having good dispersion and stability in which adding a water-soluble soybean polysaccharide and gum arabic are added, in which aggregation and sedimentation of the protein are suppressed more effectively than in the past.

Description

酸性蛋白質飲料及びその製造方法Acidic protein beverage and method for producing the same
 本発明は、酸性蛋白質飲料及びその製造方法に関する。 The present invention relates to an acidic protein beverage and a method for producing the same.
 蛋白質や乳酸菌(死菌含む)の健康維持に関する有用性が示されている。これに伴い、乳酸菌飲料や殺菌乳酸菌飲料の酸性蛋白質飲料市場は拡大している。これらは主に乳を原料としており、乳原料を酸性化させると、乳原料中の蛋白質が凝集するという問題点が存在する。これらに対する解決策としては、ハイメトキシルペクチン(以下、HMペクチン)やカルボキシメチルセルロースナトリウム(以下、CMC-Na)、水溶性大豆多糖類などの安定剤を単独またはこれらの組み合わせやタマリンドシードガムなど他の多糖類を適宜組み合わせて、配合することが広く行われている。(特許文献1~4) It has been shown to be useful for maintaining the health of proteins and lactic acid bacteria (including dead bacteria). Along with this, the acidic protein beverage market for lactic acid bacteria beverages and sterilized lactic acid bacteria beverages is expanding. These mainly use milk as a raw material, and there is a problem that proteins in the milk raw material aggregate when the milk raw material is acidified. Solutions to these include stabilizers such as high methoxyl pectin (hereinafter referred to as HM pectin), sodium carboxymethylcellulose (hereinafter referred to as CMC-Na), water-soluble soybean polysaccharides alone or combinations thereof, and other types such as tamarind seed gum. It is widely practiced to mix and combine polysaccharides as appropriate. (Patent Documents 1 to 4)
 しかし、水溶性大豆多糖類以外の安定剤で安定化した酸性蛋白飲料や水溶性大豆多糖類と他の安定剤との組み合わせで安定化させた飲料は水溶性大豆多糖類のみで安定化させた飲料と比較すると粘度が高く、飲み口の重い食感になる。これは組み合わせる安定剤の粘度に由来する為、これまで報告されてきた素材で安定性を確保しつつ、低粘度化することは難しい。 However, acidic protein beverages stabilized with stabilizers other than water-soluble soybean polysaccharides and beverages stabilized with a combination of water-soluble soybean polysaccharides and other stabilizers were stabilized only with water-soluble soybean polysaccharides. Compared with beverages, the viscosity is high and the mouthfeel is heavy. Since this is derived from the viscosity of the stabilizer to be combined, it is difficult to reduce the viscosity while ensuring stability with the materials reported so far.
 特許文献5にはペクチンとアラビアガムを併用することで高い安定性を有し、且つ糊状感の無い飲料を調製できると記載がある。しかし、特許文献5の技術においても飲料の粘度は高く、すっきりとした飲み口とは言い難く、また、安定性についても不十分であり、さらなる改善が望まれている。 Patent Document 5 describes that a beverage having high stability and having no pasty feeling can be prepared by using pectin and gum arabic in combination. However, even in the technique of Patent Document 5, the viscosity of the beverage is high, it is difficult to say that it is a refreshing mouth, and the stability is insufficient, and further improvement is desired.
特開平5-7458号公報Japanese Patent Laid-Open No. 5-7458 特開2005-245217号公報JP 2005-245217 A 特開2005-40024号公報JP 2005-40024 JP 特開2006-217831号公報JP 2006-217831 A 特開平10-313781号公報JP-A-10-313781
 このように、これまでの多糖類単独やそれらの製剤では、飲み口及び安定性の点で必ずしも満足できる酸性蛋白質飲料は得られていない。
 本発明は、低粘度の酸性蛋白質飲料について、従来よりも蛋白質を分散・安定化させる機能を向上し、飲み口の軽い、すっきりとした飲料を提供することを課題とする。
Thus, conventional polysaccharides alone and their preparations do not necessarily provide acidic protein beverages that are satisfactory in terms of drinking mouth and stability.
It is an object of the present invention to provide a refreshing beverage with a light mouthfeel, improving the function of dispersing and stabilizing the protein as compared with conventional acidic protein beverages having a low viscosity.
 本発明者らは、上記の課題に対して鋭意研究を重ねた結果、水溶性大豆多糖類及びアラビアガムを添加することで、低粘度で、従来よりも蛋白質の凝集や沈殿が優位に抑制された分散安定性の良好な酸性蛋白質飲料を提供できることを見出し、本発明を完成させた。 As a result of intensive research on the above problems, the present inventors have added water-soluble soy polysaccharide and gum arabic, which has a low viscosity and suppresses the aggregation and precipitation of proteins. The present inventors have found that an acidic protein beverage with good dispersion stability can be provided.
即ち、本発明は、
(1)水溶性大豆多糖類及びアラビアガムを含有するpH3.3~4.4の酸性蛋白質飲料、
(2)蛋白質を0.01~2重量%含有する、(1)記載の酸性蛋白質飲料、
(3)飲料に対して、水溶性大豆多糖類を0.01~1重量%、アラビアガムを0.05~1重量%含有する(1)又は(2)記載の酸性蛋白質飲料、
(4)酸性蛋白質飲料の粘度が、B型回転粘度計で、ローターNo.19、60rpm、1分、20℃の条件で測定したときに、5mPa・s以下である、(1)~(3)の何れか1項に記載の酸性蛋白質飲料、
(5)水溶性大豆多糖類及びアラビアガムを添加することを特徴とする、pH3.3~4.4の酸性蛋白質飲料の製造方法、
(6)飲料に対して、水溶性大豆多糖類を0.01~1重量%、アラビアガムを0.05~1重量%添加する、(5)記載の酸性蛋白質飲料の製造方法、
(7)水溶性大豆多糖類及びアラビアガムを添加することを特徴とする、pH3.3~4.4の酸性蛋白質飲料の分散安定化方法、
である。
That is, the present invention
(1) an acidic protein beverage having a pH of 3.3 to 4.4 containing a water-soluble soybean polysaccharide and gum arabic;
(2) The acidic protein beverage according to (1), containing 0.01 to 2% by weight of protein,
(3) The acidic protein beverage according to (1) or (2), containing 0.01 to 1% by weight of water-soluble soybean polysaccharide and 0.05 to 1% by weight of gum arabic with respect to the beverage,
(4) The viscosity of the acidic protein beverage is a B-type rotational viscometer. The acidic protein beverage according to any one of (1) to (3), which is 5 mPa · s or less when measured under conditions of 19, 60 rpm, 1 minute, and 20 ° C.,
(5) A method for producing an acidic protein beverage having a pH of 3.3 to 4.4, comprising adding a water-soluble soybean polysaccharide and gum arabic,
(6) A method for producing an acidic protein beverage according to (5), wherein 0.01 to 1% by weight of water-soluble soybean polysaccharide and 0.05 to 1% by weight of gum arabic are added to the beverage,
(7) A method for stabilizing the dispersion of an acidic protein beverage having a pH of 3.3 to 4.4, comprising adding a water-soluble soybean polysaccharide and gum arabic,
It is.
 本発明により、低粘度ですっきりとした飲み口で、従来よりも蛋白質の凝集や沈殿を大幅に抑制された分散安定性の良好な飲料を提供することができる。 According to the present invention, it is possible to provide a beverage having a good dispersion stability in which protein aggregation and precipitation are significantly suppressed as compared with conventional ones with a clean mouth with low viscosity.
 以下、本発明を具体的に説明する。 Hereinafter, the present invention will be specifically described.
(酸性蛋白質飲料)
 本発明の酸性蛋白質飲料は、水溶性大豆多糖類及びアラビアガムを含有することを特徴とする。
 本発明における酸性蛋白質飲料とは、牛乳、全脂粉乳、加糖練乳、脱脂粉乳、加糖脱脂練乳、脱脂濃縮乳や豆乳、大豆蛋白質水溶液等、蛋白質含有溶液に乳酸やクエン酸等の有機酸を添加することで酸性化した直接酸性化飲料もしくは前述した蛋白質含有溶液を乳酸菌などで発酵して調製されるドリンクヨーグルトや乳酸菌飲料など発酵乳飲料及びそれらを凍結させたフローズンヨーグルトなどが挙げられる。
 酸性条件についてはpH3.3~pH4.4のものが挙げられる。pH3.2以下になると飲料に供される蛋白質は殆ど可溶化する為、水溶性大豆多糖類及びアラビアガムは不要になる。逆にpH4.5以上でも効果は見られるが、水溶性大豆多糖類及びアラビアガムの添加量が非常に多くなるため、飲料製造コストの上昇や粘度が高まる為、嗜好性に劣る点から好ましくない場合がある。飲料の粘度を高めることなく、安定化可能な酸性蛋白質飲料中の蛋白質含有量としては、好ましくは2重量%以下、より好ましくは1.5重量%以下、さらにより好ましくは1重量%以下である。また、0.01重量%以上が好ましく、0.05重量%以上がより好ましい。
 2重量%よりも多量の蛋白質含有量でも安定化は可能だが、安定化に必要な水溶性大豆多糖類及びアラビアガムの添加量が増え、コストや飲料の粘度の上昇などが生じる場合があり、本発明の目的であるすっきりとした飲料を調製する上で好ましくない場合がある。また、0.01重量%未満では凝集の問題が起こりにくい場合があり、水溶性大豆多糖類及びアラビアガムの添加が不要になる場合がある。
 このように、飲料をすっきりとした飲み口のものにするために粘度は重要であり、B型回転粘度計(東機産業(株)製)を用い、ローターNo.19、60rpm、1分、20℃の条件で測定したときの粘度が、好ましくは5mPa・s以下、より好ましくは3mPa・s以下、さらに好ましくは2mPa・s以下である。
 本発明の水溶性大豆多糖類及びアラビアガムを含有する酸性蛋白質飲料は、pH3.3~4.4の酸性下で、低粘度ですっきりとした飲み口で、従来よりも蛋白質の凝集や沈殿を大幅に抑制され、分散安定性がより良好となる。水溶性大豆多糖類あるいはアラビアガムを単独で用いた場合、分散安定性の面で目標とする品質が得られない。
(Acid protein drink)
The acidic protein beverage of the present invention is characterized by containing a water-soluble soybean polysaccharide and gum arabic.
The acidic protein beverage in the present invention is an addition of an organic acid such as lactic acid or citric acid to a protein-containing solution such as milk, whole milk powder, sweetened condensed milk, skimmed powdered milk, sweetened defatted condensed milk, defatted concentrated milk, soy milk, soy protein aqueous solution, etc. Examples thereof include a directly acidified beverage acidified by fermentation, a fermented milk beverage such as a drink yogurt or a lactic acid bacteria beverage prepared by fermenting the above-described protein-containing solution with lactic acid bacteria, and frozen yogurt obtained by freezing them.
Examples of acidic conditions include those having a pH of 3.3 to 4.4. When the pH is 3.2 or lower, the protein used in the beverage is almost solubilized, so that the water-soluble soybean polysaccharide and gum arabic are no longer necessary. On the contrary, the effect is seen even at pH 4.5 or more, but since the addition amount of water-soluble soybean polysaccharide and gum arabic is very large, the beverage production cost is increased and the viscosity is increased. There is a case. The protein content in the acidic protein beverage that can be stabilized without increasing the viscosity of the beverage is preferably 2% by weight or less, more preferably 1.5% by weight or less, and even more preferably 1% by weight or less. . Moreover, 0.01 weight% or more is preferable and 0.05 weight% or more is more preferable.
Stabilization is possible even with a protein content higher than 2% by weight, but the amount of water-soluble soybean polysaccharide and gum arabic required for stabilization increases, which may increase costs and viscosity of the beverage. It may not be preferable in preparing a refreshing beverage that is the object of the present invention. Further, if it is less than 0.01% by weight, the problem of aggregation may hardly occur, and the addition of water-soluble soybean polysaccharide and gum arabic may be unnecessary.
Thus, the viscosity is important in order to make the beverage a refreshing drink, and using a B-type rotational viscometer (manufactured by Toki Sangyo Co., Ltd.), the rotor No. The viscosity when measured under the conditions of 19, 60 rpm, 1 minute, and 20 ° C. is preferably 5 mPa · s or less, more preferably 3 mPa · s or less, and even more preferably 2 mPa · s or less.
The acidic protein beverage containing the water-soluble soybean polysaccharide and gum arabic of the present invention has a low-viscosity, clean drinking mouth under the acidity of pH 3.3 to 4.4, and more protein aggregation and precipitation than before. It is greatly suppressed and the dispersion stability becomes better. When water-soluble soybean polysaccharide or gum arabic is used alone, the target quality cannot be obtained in terms of dispersion stability.
(水溶性大豆多糖類)
 本発明における水溶性大豆多糖類は、種々の方法で得ることができ、例えば特許第2599477号に記載された方法で得ることができる。製造方法の一例を示せば、分離大豆蛋白質の製造工程で得られた生オカラに2倍重量部の水を加え、塩酸を用いてpHを4.5に調整する。120℃で90分間加圧加熱処理を行ない、冷却後に遠心分離(10000×g、20分間)により不溶性成分を分離して水溶性大豆多糖類溶液を得る。同多糖類溶液を最終60重量%エタノールで沈殿させ、回収した沈殿を90重量%の含水エタノールで洗浄し、得られた沈殿を風乾して水溶性大豆多糖類を得ることが出来る。
(Water-soluble soybean polysaccharide)
The water-soluble soybean polysaccharide in the present invention can be obtained by various methods, for example, by the method described in Japanese Patent No. 2599477. If an example of a manufacturing method is shown, 2 times weight part water will be added to the raw okara obtained by the manufacturing process of isolation | separation soybean protein, and pH will be adjusted to 4.5 using hydrochloric acid. A pressure heat treatment is performed at 120 ° C. for 90 minutes, and after cooling, insoluble components are separated by centrifugation (10000 × g, 20 minutes) to obtain a water-soluble soybean polysaccharide solution. The polysaccharide solution is precipitated with 60% by weight of ethanol at the end, the recovered precipitate is washed with 90% by weight of water-containing ethanol, and the resulting precipitate is air-dried to obtain a water-soluble soybean polysaccharide.
 水溶性大豆多糖類の添加量は飲料中の蛋白質含量にもよるが、飲料に対して、好ましくは0.01~1重量%、より好ましくは0.02~0.7重量%、さらにより好ましくは0.1~0.4重量%である。0.01重量%未満では蛋白質の分散安定効果が不十分になる場合があり、逆に1重量%を超えると製造コストが高くなるため、好ましくない場合がある。 The amount of water-soluble soybean polysaccharide added depends on the protein content in the beverage, but is preferably 0.01 to 1% by weight, more preferably 0.02 to 0.7% by weight, and still more preferably based on the beverage. Is 0.1 to 0.4% by weight. If the amount is less than 0.01% by weight, the protein dispersion stabilizing effect may be insufficient. Conversely, if the amount exceeds 1% by weight, the production cost increases, which may not be preferable.
(アラビアガム)
 本発明に使用するアラビアガムとは、Acacia senegalやAcacia seyal等、起源種に関わらず用いることが出来る。かかるアラビアガムは商業的に入手可能であり、例えば三栄源エフ・エフ・アイ株式会社製の“ガムアラビックSD”やTIC GUM社製の“ティカシアガム SD-R-N”などを挙げることが出来る。
(Gum Arabic)
The gum arabic used in the present invention can be used regardless of the origin species such as Acacia senegal and Acacia sial. Such gum arabic is commercially available. Examples thereof include “Gum Arabic SD” manufactured by San-Ei Gen FFI Co., Ltd. and “Tikacia Gum SD-RN” manufactured by TIC GUM.
 アラビアガムの添加量は飲料中の蛋白質含量及び配合する水溶性大豆多糖類の添加量にもよるが、飲料に対して、好ましくは0.05~1重量%、より好ましくは0.1~0.7重量%、さらにより好ましくは0.2~0.5重量%である。0.05重量%未満では蛋白質の分散安定効果が不十分になる場合があり、逆に1重量%を超えると製造コストが高くなるため、好ましくない場合がある。 The amount of gum arabic added depends on the protein content in the beverage and the amount of water-soluble soybean polysaccharide to be added, but is preferably 0.05 to 1% by weight, more preferably 0.1 to 0%, based on the beverage. 0.7% by weight, still more preferably 0.2-0.5% by weight. If the amount is less than 0.05% by weight, the protein dispersion stabilizing effect may be insufficient. On the other hand, if it exceeds 1% by weight, the production cost increases, which may be undesirable.
 本発明の酸性蛋白質飲料は、水溶性大豆多糖類とアラビアガムを配合することが必須であるが、本発明の効果に悪影響を与えない範囲で適宜他の素材を併用しても良い。他の素材としては、安定剤として寒天、カラギーナン、ファーセレラン、タマリンド種子多糖類、タラガム、カラヤガム、ペクチン、キサンタンガム、アルギン酸ナトリウム、トラガントガム、グァーガム、ローカストビーンガム、プルラン、ジェランガム、カルボキシメチルセルロース(CMC)、アルギン酸プロピレングリコールエステル、加工澱粉など各種多糖類が挙げられる。
 更に本発明の効果に悪影響を与えない範囲で必要に応じて各種食品原料や食品添加物を併用出来る。食品原料としては、蔗糖、砂糖、果糖ブドウ糖液糖、デキストリン、マルトース、トレハロ─ ス、ソルビトール、キシリトール、マルチトール等の糖質及び糖アルコ─ ル、食品添加物としてはグリセリン脂肪酸エステル、ショ糖脂肪酸エステル等の乳化剤、カルシウム強化剤等の塩類、クエン酸ナトリウムや酢酸ナトリウム等の有機酸塩、安息香酸、ソルビン酸、しらこ蛋白、グリシン等の保存料などが挙げられる。
In the acidic protein beverage of the present invention, it is essential to mix water-soluble soybean polysaccharide and gum arabic, but other materials may be used in combination as long as the effects of the present invention are not adversely affected. Other materials include agar, carrageenan, farseleran, tamarind seed polysaccharide, tara gum, karaya gum, pectin, xanthan gum, sodium alginate, tragacanth gum, guar gum, locust bean gum, pullulan, gellan gum, carboxymethylcellulose (CMC), alginic acid as stabilizers. Various polysaccharides such as propylene glycol ester and processed starch are listed.
Furthermore, various food raw materials and food additives can be used together as necessary within a range that does not adversely affect the effects of the present invention. Food ingredients include sucrose, sugar, fructose, glucose liquid sugar, dextrin, maltose, trehalose, sorbitol, xylitol, maltitol and other sugars and sugar alcohols, and food additives include glycerin fatty acid esters and sucrose fatty acids. Examples include emulsifiers such as esters, salts such as calcium fortifiers, organic acid salts such as sodium citrate and sodium acetate, preservatives such as benzoic acid, sorbic acid, shirako protein, and glycine.
(酸性蛋白質飲料の製造方法)
 また、本発明の酸性蛋白質飲料の調製方法としては、水溶性大豆多糖類及びアラビアガムを含むように調製されていれば特に限定されないが、例えば蛋白質含有溶液と、水溶性大豆多糖類とアラビアガムを含む水溶液を夫々調製した後混合し、混合液を乳酸やクエン酸等の有機酸でpHを3.3~4.4に調整し、均質化工程、加熱殺菌工程、容器充填工程を経て製造する方法や、蛋白質含有溶液を乳酸菌で発酵してpH3.3~4.4に調整し、発酵液を均質化して得られた発酵乳と、水溶性大豆多糖類とアラビアガムを含む水溶液を夫々調製した後混合し、混合液を均質化工程、加熱殺菌工程、容器充填工程を経て製造する方法等が挙げられる。
(Method for producing acidic protein beverage)
The method for preparing the acidic protein beverage of the present invention is not particularly limited as long as it is prepared so as to contain a water-soluble soybean polysaccharide and gum arabic. For example, a protein-containing solution, a water-soluble soybean polysaccharide and gum arabic are prepared. After preparing each aqueous solution containing water, the pH of the mixture is adjusted to 3.3 to 4.4 with an organic acid such as lactic acid or citric acid, and manufactured through a homogenization process, a heat sterilization process, and a container filling process. A fermented milk obtained by fermenting a protein-containing solution with lactic acid bacteria to a pH of 3.3 to 4.4 and homogenizing the fermented solution, and an aqueous solution containing a water-soluble soybean polysaccharide and gum arabic, respectively. Examples include a method of preparing and mixing and preparing a mixed solution through a homogenization step, a heat sterilization step, and a container filling step.
 以下、実施例、比較例により本発明の実施態様を説明するが、本発明はこれらの例示によって制限されるものではない。なお、例中%は何れも重量基準を意味する。 Hereinafter, embodiments of the present invention will be described with reference to Examples and Comparative Examples, but the present invention is not limited to these exemplifications. In the examples,% means weight basis.
 以下に示す実施例において水溶性大豆多糖類としては「ソヤファイブ-S」(不二製油(株)製)を使用した。またアラビアガムは市販されている「ティカシアガム SD-R-N」(TIC GUM社製)を使用した。 In the following examples, “Soya Five-S” (produced by Fuji Oil Co., Ltd.) was used as the water-soluble soybean polysaccharide. As gum arabic, a commercially available “Tikacia gum SD-RN” (manufactured by TIC GUM) was used.
(酸性蛋白質飲料の評価方法)
 以下に示す方法で酸性蛋白質飲料を調製し、調製後5℃で1週間保存後の飲料について、pH、粘度、沈殿率、粒度分布(メジアン径)について評価を行った。
○pH
 20℃にてpH METER F-71((株)堀場製作所製)で測定した。
○粘度
B型回転粘度計(東機産業(株)製)を用い、ローターNo.19、60rpm、1分、20℃の条件で測定した。
○粒度分布(メジアン径)
 SALD-2000((株)島津製作所製)にて測定した。
○沈殿率
遠沈管に飲料を容器に一定量分取したサンプル量と、分取したサンプルを遠心分離器で遠心分離後(700×g、20分)、上澄みを廃棄した後の沈殿量から算出した。すなわち、
沈殿率(%)=(沈殿重量/サンプル重量)×100 の式より算出した。
(Evaluation method for acidic protein beverages)
An acidic protein beverage was prepared by the method described below, and the pH, viscosity, precipitation rate, and particle size distribution (median diameter) of the beverage after preparation and storage at 5 ° C. for 1 week were evaluated.
○ pH
The measurement was performed at 20 ° C. with pH METER F-71 (manufactured by Horiba, Ltd.).
○ Viscosity Using a B-type rotational viscometer (manufactured by Toki Sangyo Co., Ltd.), rotor No. The measurement was performed at 19, 60 rpm, 1 minute, and 20 ° C.
○ Particle size distribution (median diameter)
Measurement was performed with SALD-2000 (manufactured by Shimadzu Corporation).
○ Precipitation rate Calculated from the amount of sample obtained by dispensing a certain amount of beverage into a centrifuge tube, and the amount of precipitate after centrifuging the collected sample with a centrifuge (700 × g, 20 minutes) and discarding the supernatant did. That is,
Precipitation rate (%) = (precipitation weight / sample weight) × 100.
 調製した飲料の粘度が、5mPa・s以下、粒度分布(メジアン径)が、1.2μm以下且つ沈殿率が1.2%以下のものを低粘度で飲料安定性に優れた品質として合格品質とした。 The prepared beverage has a viscosity of 5 mPa · s or less, a particle size distribution (median diameter) of 1.2 μm or less, and a precipitation rate of 1.2% or less as a quality that has low viscosity and excellent beverage stability. did.
(殺菌酸性蛋白質飲料の調製) 脱脂粉乳100gを常温水900gに加え、70℃、20分間撹拌溶解し、冷却後、10%脱脂粉乳溶液を得た。砂糖500gを常温の水500gに加え撹拌溶解し、50%糖液を得た。ソヤファイブ-S50gを水950gに加え、80℃、20分間で撹拌溶解後、20℃まで冷却し、5%ソヤファイブ-S溶液を得た。ティカシアガム SD-R-N50gを水950gに加え、80℃、20分間で撹拌溶解後、冷却し、5%ティカシアガム溶液を得た。これらを原料液とし、以下の実施例及び比較例の条件にて飲料を調製した。 (Preparation of Bactericidal Acidic Protein Beverage) 100 g of skim milk powder was added to 900 g of room temperature water, dissolved by stirring at 70 ° C. for 20 minutes, and after cooling, a 10% skim milk powder solution was obtained. 500 g of sugar was added to 500 g of water at room temperature and dissolved by stirring to obtain a 50% sugar solution. 50 g of Soya Five-S was added to 950 g of water, dissolved by stirring at 80 ° C. for 20 minutes, and then cooled to 20 ° C. to obtain a 5% Soya Five-S solution. Ticacia gum SD-R-N (50 g) was added to 950 g of water, dissolved by stirring at 80 ° C. for 20 minutes, and then cooled to obtain a 5% ticasia gum solution. Using these as raw material liquids, beverages were prepared under the conditions of the following Examples and Comparative Examples.
(実施例1)蛋白質1.0%含有殺菌酸性蛋白質飲料の調製
 表1の配合に従い原料液を混合し、50%クエン酸溶液でpHを4.0に調整した後、水で全量を100%とし、150kg/cm2にて均質化を施した。均質化された溶液を90℃,20分間の条件にて殺菌し、容器に充填後、冷却し、蛋白質1%を含有する酸性蛋白質飲料を得た。
Example 1 Preparation of Sterilized Acidic Protein Beverage Containing 1.0% Protein After mixing the raw material liquids according to the formulation shown in Table 1, adjusting the pH to 4.0 with a 50% citric acid solution, the total amount is 100% with water. And homogenization was performed at 150 kg / cm 2 . The homogenized solution was sterilized at 90 ° C. for 20 minutes, filled into a container, and then cooled to obtain an acidic protein beverage containing 1% protein.
(比較例1)
 実施例1において、5%ティカシアガム溶液を除き、安定剤としてソヤファイブ-Sのみを用いた点以外は全く同様にして飲料を調製した。
(Comparative Example 1)
A beverage was prepared in the same manner as in Example 1 except that 5% ticasia gum solution was used and only Soya Five-S was used as a stabilizer.
(比較例2)
 実施例1において、5%ソヤファイブ-S溶液を除き、安定剤としてティカシアガムのみを用いた点以外は全く同様にして飲料を調製した。
(Comparative Example 2)
A beverage was prepared in the same manner as in Example 1, except that 5% Soyafive-S solution was used and only ticasia gum was used as a stabilizer.
(実施例2)
 表2の配合に従い原料液を混合し、50%クエン酸溶液でpHを4.4に調整した後、水で全量を100%とし、150kg/cm2にて均質化を施した。均質化された溶液を90℃,20分間の条件にて殺菌し、容器に充填後、冷却し、飲料を得た。
(Example 2)
The raw material liquids were mixed according to the formulation shown in Table 2, and the pH was adjusted to 4.4 with a 50% citric acid solution. The total amount was adjusted to 100% with water and homogenized at 150 kg / cm 2 . The homogenized solution was sterilized at 90 ° C. for 20 minutes, filled into a container, and then cooled to obtain a beverage.
(比較例3)
 実施例2において、5%ティカシアガム溶液を除き、安定剤としてソヤファイブ-Sのみを用いた点以外は全く同様にして飲料を調製した。
(Comparative Example 3)
In Example 2, a beverage was prepared in exactly the same manner except that 5% ticasia gum solution was used and only Soyafive-S was used as a stabilizer.
(比較例4)
 実施例2において、5%ソヤファイブ-S溶液を除き、安定剤としてティカシアガムのみを用いた点以外は全く同様にして飲料を調製した。
(Comparative Example 4)
In Example 2, a beverage was prepared in exactly the same manner except that 5% Soyafive-S solution was used and only ticasia gum was used as a stabilizer.
(表1)
Figure JPOXMLDOC01-appb-I000001
(Table 1)
Figure JPOXMLDOC01-appb-I000001
(表2)
Figure JPOXMLDOC01-appb-I000002
(Table 2)
Figure JPOXMLDOC01-appb-I000002
(安定性評価結果)
 各酸性蛋白質飲料の分析結果を表2に示した。ソヤファイブ-S単独(比較例1、3)およびティカシアガム単独(比較例2、4)において、蛋白質粒子のメジアン径は1.897~90.70μmと大きく、沈殿率も1.530~11.07%と非常に高いことから、飲料として安定性が不十分であり、品質は不合格であった。一方、ソヤファイブ-Sとティカシアガムを併用することで(実施例1、2)、蛋白質粒子のメジアン径は0.869~1.095μmと比較例よりも微細化されており、また沈殿率も0.573~1.005%と、著しく改善され、併用によりメジアン径1.2μm以下且つ沈殿率1.2%以下の、良好な品質のものを得ることができた。またソヤファイブ-Sとアラビアガムを併用した飲料は粘度5mPa・s以下であることからも分かる様に、すっきりとした良好な飲み口であった。
(Stability evaluation result)
The analysis results for each acidic protein beverage are shown in Table 2. In Soya Five-S alone (Comparative Examples 1 and 3) and Tikacia Gum alone (Comparative Examples 2 and 4), the median diameter of the protein particles is as large as 1.897 to 90.70 μm, and the precipitation rate is 1.530 to 11.07%. The stability was insufficient as a beverage and the quality was unacceptable. On the other hand, when Soya Five-S and ticasia gum are used in combination (Examples 1 and 2), the median diameter of the protein particles is 0.869 to 1.095 μm, which is finer than that of the comparative example, and the precipitation rate is also 0.1. It was markedly improved to 573 to 1.005%, and when used in combination, a good quality product having a median diameter of 1.2 μm or less and a precipitation rate of 1.2% or less could be obtained. In addition, as the beverage using Soya Five-S and gum arabic in combination had a viscosity of 5 mPa · s or less, it was a clean and good mouth.
(実施例3)蛋白質0.3%含有殺菌酸性蛋白質飲料の調製
 表3の配合に従い原料液を混合し、50%クエン酸溶液でpHを4.2に調整した後、水で全量を100%とし、150kg/cm2にて均質化を施した。均質化された溶液を90℃,20分間の条件にて殺菌し、容器に充填後、冷却し、蛋白質0.3%を含有する酸性蛋白質飲料を得た。
Example 3 Preparation of Sterilized Acidic Protein Beverage Containing 0.3% Protein After mixing raw material liquids according to the formulation shown in Table 3 and adjusting the pH to 4.2 with 50% citric acid solution, the total amount is 100% with water. And homogenization was performed at 150 kg / cm 2 . The homogenized solution was sterilized at 90 ° C. for 20 minutes, filled into a container and then cooled to obtain an acidic protein beverage containing 0.3% protein.
(比較例5)
 実施例3において、5%ティカシアガム溶液を除き、安定剤としてソヤファイブ-Sのみを用いた点以外は全く同様にして飲料を調製した。
(Comparative Example 5)
A beverage was prepared in exactly the same manner as in Example 3 except that 5% ticasia gum solution was used and only Soya Five-S was used as a stabilizer.
(比較例6)
 実施例3において、5%ソヤファイブ-S溶液を除き、安定剤としてティカシアガムのみを用いた点以外は全く同様にして飲料を調製した。
(Comparative Example 6)
In Example 3, a beverage was prepared in exactly the same manner except that 5% Soyafive-S solution was used and only ticasia gum was used as a stabilizer.
(表3)
Figure JPOXMLDOC01-appb-I000003
(Table 3)
Figure JPOXMLDOC01-appb-I000003
(安定性評価結果)
 各酸性蛋白質飲料の分析結果を表3に示した。ソヤファイブ-S単独(比較例5)、ティカシアガム単独(比較例6)において、蛋白質粒子のメジアン径は1.932~46.12μmと大きく、沈殿率も2%以上と高いことから、飲料として安定性が不十分であり、品質不合格と言える。一方、ソヤファイブ-Sとティカシアガムを併用することで(実施例3)、蛋白質粒子のメジアン径は0.563μmと微細化され、沈殿率も0.184%と、著しく改善され、併用により良好な品質のものを得ることができた。また併用による飲料の増粘は無く、すっきりとした飲み口の飲料であった。
(Stability evaluation result)
The analysis results of each acidic protein beverage are shown in Table 3. In Soya Five-S alone (Comparative Example 5) and Tikacia Gum alone (Comparative Example 6), the median diameter of the protein particles is as large as 1.932 to 46.12 μm and the precipitation rate is as high as 2% or more. Is insufficient, and it can be said that the quality is not acceptable. On the other hand, by using Soya Five-S and Ticasia gum together (Example 3), the median diameter of the protein particles was refined to 0.563 μm and the precipitation rate was significantly improved to 0.184%. Could get things. Moreover, there was no thickening of the drink by combined use and it was a refreshing drink.
(乳酸菌飲料の調製)
(原料液の調製)
 脱脂粉乳15%にグルコース5%を加え、水で全量を100%とした後、90℃で30分間殺菌を行い、15%脱脂粉乳溶液を調製した。調製した溶液に発酵スターターとしてL.caseiを2.5ppm添加し、38℃で120時間発酵し、得られた発酵液をミキサーにて破砕しながら、5℃まで氷冷後、150kg/cm2で均質化を行い、発酵乳を得た。得られた発酵乳のpHは3.6であった。尚、発酵を停止させる為、得られた発酵乳は5℃で保管した。
 次に、砂糖500gを常温の水500gに加え撹拌溶解し、50%糖液を得た。ソヤファイブ-S50gを水950gに加え、80℃,20分間で撹拌溶解後、20℃まで冷却し、5%ソヤファイブ-S溶液を得た。ティカシアガム SD-R-N50gを水950gに加え、80℃,20分間で撹拌溶解後、冷却し、5%ティカシアガム溶液を得た。これらを原料液とし、以下の実施例及び比較例の条件にて飲料を調製した。
(Preparation of lactic acid bacteria beverage)
(Preparation of raw material liquid)
Glucose 5% was added to skim milk powder 15%, and the total amount was adjusted to 100% with water, followed by sterilization at 90 ° C. for 30 minutes to prepare a 15% skim milk powder solution. As a fermentation starter, L. Casei was added at 2.5 ppm, fermented at 38 ° C. for 120 hours, and the resulting fermentation broth was crushed with a mixer, ice-cooled to 5 ° C., and then homogenized at 150 kg / cm 2 to obtain fermented milk. It was. The pH of the obtained fermented milk was 3.6. In addition, in order to stop fermentation, the obtained fermented milk was stored at 5 degreeC.
Next, 500 g of sugar was added to 500 g of water at room temperature and dissolved by stirring to obtain a 50% sugar solution. 50 g of Soya Five-S was added to 950 g of water, stirred and dissolved at 80 ° C. for 20 minutes, and then cooled to 20 ° C. to obtain a 5% Soya Five-S solution. Ticacia gum SD-R-N (50 g) was added to 950 g of water, dissolved by stirring at 80 ° C. for 20 minutes, and then cooled to obtain a 5% ticasia gum solution. Using these as raw material liquids, beverages were prepared under the conditions of the following Examples and Comparative Examples.
(実施例4)蛋白質1.0%含有乳酸菌飲料の調製
 表4の配合に従い原料液を混合し、水で全量を100%とし、150kg/cm2にて均質化を施した。均質化された溶液を容器に充填後、蛋白質1.0%を含有する乳酸菌飲料を得た。
(Example 4) raw material liquid mixed in accordance with the formulation of the preparation Table 4 Protein 1.0% content lactobacillus beverage, the total amount of water as 100%, was subjected to homogenization at 150 kg / cm 2. After filling the homogenized solution into a container, a lactic acid bacteria beverage containing 1.0% protein was obtained.
(実施例5)
 実施例4において5%ティカシアガム溶液を6.0%から2.0%へ添加量を減じた点以外は全く同様にして飲料を調製した。
(Example 5)
A beverage was prepared in the same manner as in Example 4 except that the addition amount of the 5% ticasia gum solution was reduced from 6.0% to 2.0%.
(比較例7)
 実施例4において、5%ティカシアガム溶液を除き、安定剤としてソヤファイブ-Sのみを用いた点以外は全く同様にして飲料を調製した。
(Comparative Example 7)
A beverage was prepared in the same manner as in Example 4 except that 5% ticasia gum solution was used and only Soya Five-S was used as a stabilizer.
(比較例8)
 実施例4において、5%ソヤファイブ-S溶液を除き、安定剤としてティカシアガムのみを用いた点以外は全く同様にして飲料を調製した。
(Comparative Example 8)
In Example 4, a beverage was prepared in exactly the same manner except that 5% Soyafive-S solution was used and only ticasia gum was used as a stabilizer.
(表4)
Figure JPOXMLDOC01-appb-I000004
(Table 4)
Figure JPOXMLDOC01-appb-I000004
(安定性評価結果)
 各酸性蛋白質飲料の分析結果を表4に示した。ソヤファイブ-S単独(比較例7)では沈殿率が1.195%、粘度が2.10μmと合格値であるものの、蛋白質粒子のメジアン径は4.445μmと大きく、品質は不合格であった。またティカシアガム単独(比較例8)において、蛋白質粒子のメジアン径は82.67μmと非常に大きく、また沈殿率も12.89%と高いことから、飲料として安定性が不十分であり、品質は不合格であった。
 一方、ソヤファイブ-Sとティカシアガムを併用することで(実施例4、5)、蛋白質粒子のメジアン径は0.957~1.179μmと微細化され、沈殿率も0.470~0.513%と、比較例と比べて著しく改善され、良好な品質のものを得ることができた。また実施例4、5においても併用による飲料の増粘は無く、すっきりとした飲み口であった。
(Stability evaluation result)
The analysis results for each acidic protein beverage are shown in Table 4. With Soya Five-S alone (Comparative Example 7), although the precipitation rate was 1.195% and the viscosity was 2.10 μm, which was acceptable, the median diameter of the protein particles was as large as 4.445 μm, and the quality was not acceptable. In addition, in the ticasia gum alone (Comparative Example 8), the median diameter of the protein particles is as very large as 82.67 μm, and the precipitation rate is as high as 12.89%. Therefore, the stability as a beverage is insufficient and the quality is poor. It was a pass.
On the other hand, when Soya Five-S and ticasia gum are used in combination (Examples 4 and 5), the median diameter of the protein particles is reduced to 0.957 to 1.179 μm, and the precipitation rate is 0.470 to 0.513%. Compared with the comparative example, it was remarkably improved and a good quality product could be obtained. Also in Examples 4 and 5, there was no thickening of the beverage due to the combined use, and it was a clean drinking mouth.
(実施例6)蛋白質0.3%含有乳酸菌飲料の調製
 表5の配合に従い原料液を混合し、水で全量を100%とし、150kg/cm2にて均質化を施した。均質化された溶液を容器に充填後、蛋白質0.3%を含有する乳酸菌飲料を得た。
Example 6 Preparation of 0.3% Protein-Containing Lactic Acid Beverage Beverages The raw material liquids were mixed according to the formulation shown in Table 5, and the total amount was made 100% with water, and homogenized at 150 kg / cm 2 . After filling the homogenized solution into a container, a lactic acid bacteria beverage containing 0.3% protein was obtained.
(比較例9)
 実施例6において、5%ティカシアガム溶液を除き、安定剤としてソヤファイブ-Sのみを用いた点以外は全く同様にして飲料を調製した。
(Comparative Example 9)
In Example 6, a beverage was prepared in exactly the same manner except that 5% ticasia gum solution was used and only Soya Five-S was used as a stabilizer.
(比較例10)
 実施例6において、5%ソヤファイブ-S溶液を除き、安定剤としてティカシアガムのみを用いた点以外は全く同様にして飲料を調製した。
(Comparative Example 10)
A beverage was prepared in the same manner as in Example 6 except that 5% Soyafive-S solution was used and only ticasia gum was used as a stabilizer.
(表5)
Figure JPOXMLDOC01-appb-I000005
(Table 5)
Figure JPOXMLDOC01-appb-I000005
(安定性評価結果)
 各酸性蛋白質飲料の分析結果を表5に示した。ソヤファイブ-S単独(比較例9)、ティカシアガム単独(比較例10)において、蛋白質粒子のメジアン径は1.715~9.262μmと大きく、また沈殿率も2.391~2.659%と合格基準を満たさず、飲料として安定性が不十分であり、品質が不合格であった。
 一方、ソヤファイブ-Sとティカシアガムを併用することで(実施例6)、蛋白質粒子はメジアン径が0.911μmと比較例よりも小さくなり、沈殿率も0.639%と著しく改善可能され、ソヤファイブ-Sとアラビアガム併用により良好な品質のものを得ることができた。併用した飲料は粘度5mPa・s以下であることからも分かる様に、すっきりとした良好な飲み口であった。
(Stability evaluation result)
The analysis results for each acidic protein beverage are shown in Table 5. In Soya Five-S alone (Comparative Example 9) and Tikacia Gum alone (Comparative Example 10), the median diameter of the protein particles is as large as 1.715 to 9.262 μm, and the precipitation rate is 2.391 to 2.659%, which is an acceptance criterion. Was not satisfied, the stability as a beverage was insufficient, and the quality was unacceptable.
On the other hand, by using Soya Five-S and Ticacia Gum together (Example 6), the protein particle has a median diameter of 0.911 μm, which is smaller than that of the comparative example, and the precipitation rate can be remarkably improved to 0.639%. Good quality can be obtained by the combined use of S and gum arabic. As can be seen from the fact that the beverage used in combination had a viscosity of 5 mPa · s or less, it was a clean and good drinking mouth.
 以上説明した通り、本発明により、水溶性大豆多糖類とアラビアガムを酸性蛋白質飲料に使用することで、飲料の安定性を効果的に向上させ、更に他の安定剤を併用する際に見られる飲料の粘度を上昇させることなく、低粘度ですっきりとした飲料を提供することが可能になった。 As described above, according to the present invention, the use of water-soluble soybean polysaccharide and gum arabic for acidic protein beverages can effectively improve the stability of beverages, and can be seen when other stabilizers are used in combination. It has become possible to provide a refreshing beverage with a low viscosity without increasing the viscosity of the beverage.

Claims (16)

  1. 水溶性大豆多糖類及びアラビアガムを含有するpH3.3~4.4の酸性蛋白質飲料。 An acidic protein beverage having a pH of 3.3 to 4.4 containing a water-soluble soybean polysaccharide and gum arabic.
  2. 蛋白質を0.01~2重量%含有する、請求項1記載の酸性蛋白質飲料。 The acidic protein beverage according to claim 1, comprising 0.01 to 2% by weight of protein.
  3. 蛋白質を0.05~1.5重量%含有する、請求項1記載の酸性蛋白質飲料。 The acidic protein beverage according to claim 1, comprising 0.05 to 1.5% by weight of protein.
  4. 飲料に対して、水溶性大豆多糖類を0.01~1重量%、アラビアガムを0.05~1重量%含有する請求項1~3の何れか1項に記載の酸性蛋白質飲料。 The acidic protein beverage according to any one of claims 1 to 3, comprising 0.01 to 1% by weight of water-soluble soybean polysaccharide and 0.05 to 1% by weight of gum arabic with respect to the beverage.
  5. 飲料に対して、水溶性大豆多糖類を0.02~0.7重量%、アラビアガムを0.1~0.7重量%含有する請求項1~3の何れか1項に記載の酸性蛋白質飲料。 The acidic protein according to any one of claims 1 to 3, comprising 0.02 to 0.7% by weight of water-soluble soybean polysaccharide and 0.1 to 0.7% by weight of gum arabic with respect to the beverage. Beverages.
  6. 飲料に対して、水溶性大豆多糖類を0.1~0.4重量%、アラビアガムを0.2~0.5重量%含有する請求項1~3の何れか1項に記載の酸性蛋白質飲料。 The acidic protein according to any one of claims 1 to 3, comprising 0.1 to 0.4% by weight of water-soluble soybean polysaccharide and 0.2 to 0.5% by weight of gum arabic with respect to the beverage. Beverages.
  7. 酸性蛋白質飲料の粘度が、B型回転粘度計で、ローターNo.19、60rpm、1分、20℃の条件で測定したときに、5mPa・s以下である、請求項1~3の何れか1項に記載の酸性蛋白質飲料。 The viscosity of the acidic protein beverage is a B-type rotational viscometer. The acidic protein beverage according to any one of claims 1 to 3, which is 5 mPa · s or less when measured at 19, 60 rpm, 1 minute, and 20 ° C.
  8. 水溶性大豆多糖類及びアラビアガムを添加することを特徴とする、pH3.3~4.4の酸性蛋白質飲料の製造方法。 A method for producing an acidic protein beverage having a pH of 3.3 to 4.4, comprising adding a water-soluble soybean polysaccharide and gum arabic.
  9. 飲料に対して、水溶性大豆多糖類を0.01~1重量%、アラビアガムを0.05~1重量%添加する、請求項8記載の酸性蛋白質飲料の製造方法。 The method for producing an acidic protein beverage according to claim 8, wherein 0.01 to 1% by weight of water-soluble soybean polysaccharide and 0.05 to 1% by weight of gum arabic are added to the beverage.
  10. 飲料に対して、水溶性大豆多糖類を0.02~0.7重量%、アラビアガムを0.1~0.7重量%添加する、請求項8記載の酸性蛋白質飲料の製造方法。 The method for producing an acidic protein beverage according to claim 8, wherein 0.02 to 0.7% by weight of water-soluble soybean polysaccharide and 0.1 to 0.7% by weight of gum arabic are added to the beverage.
  11. 水溶性大豆多糖類及びアラビアガムを添加することを特徴とする、pH3.3~4.4の酸性蛋白質飲料の分散安定化方法。 A method for stabilizing the dispersion of an acidic protein beverage having a pH of 3.3 to 4.4, comprising adding a water-soluble soybean polysaccharide and gum arabic.
  12. 飲料に対して、水溶性大豆多糖類を0.01~1重量%、アラビアガムを0.05~1重量%添加する、請求項11記載の酸性蛋白質飲料の分散安定化方法。 The method for stabilizing the dispersion of an acidic protein beverage according to claim 11, wherein 0.01 to 1% by weight of water-soluble soybean polysaccharide and 0.05 to 1% by weight of gum arabic are added to the beverage.
  13. 飲料に対して、水溶性大豆多糖類を0.02~0.7重量%、アラビアガムを0.1~0.7重量%添加する、請求項11記載の酸性蛋白質飲料の分散安定化方法。 The method for stabilizing dispersion of an acidic protein beverage according to claim 11, wherein 0.02 to 0.7% by weight of water-soluble soybean polysaccharide and 0.1 to 0.7% by weight of gum arabic are added to the beverage.
  14. 飲料に対して、水溶性大豆多糖類を0.01~1重量%、アラビアガムを0.05~1重量%含有するpH3.3~4.4の酸性蛋白質飲料であって、該酸性蛋白質飲料の粘度がB型回転粘度計で、ローターNo.19、60rpm、1分、20℃の条件で測定したときに5mPa・s以下であり、蛋白質を0.01~2重量%含有する、酸性蛋白質飲料。 An acidic protein beverage having a pH of 3.3 to 4.4 and containing 0.01 to 1% by weight of water-soluble soybean polysaccharide and 0.05 to 1% by weight of gum arabic with respect to the beverage, The viscosity of the rotor No. is a B-type rotational viscometer. An acidic protein beverage that is 5 mPa · s or less when measured under conditions of 19, 60 rpm, 1 minute, and 20 ° C., and contains 0.01 to 2% by weight of protein.
  15. 飲料に対して、水溶性大豆多糖類を0.02~0.7重量%、アラビアガムを0.1~0.7重量%含有するpH3.3~4.4の酸性蛋白質飲料であって、該酸性蛋白質飲料の粘度がB型回転粘度計で、ローターNo.19、60rpm、1分、20℃の条件で測定したときに5mPa・s以下であり、蛋白質を0.05~1.5重量%含有する、酸性蛋白質飲料。 An acidic protein beverage having a pH of 3.3 to 4.4 containing 0.02 to 0.7% by weight of water-soluble soybean polysaccharide and 0.1 to 0.7% by weight of gum arabic with respect to the beverage, The acidic protein beverage has a B-type rotational viscometer and has a rotor No. An acidic protein beverage that is 5 mPa · s or less when measured at 19, 60 rpm, 1 minute, and 20 ° C., and contains 0.05 to 1.5% by weight of protein.
  16. 飲料に対して、水溶性大豆多糖類を0.1~0.4重量%、アラビアガムを0.2~0.5重量%含有するpH3.3~4.4の酸性蛋白質飲料であって、該酸性蛋白質飲料の粘度がB型回転粘度計で、ローターNo.19、60rpm、1分、20℃の条件で測定したときに5mPa・s以下であり、蛋白質を0.05~1.5重量%含有する、酸性蛋白質飲料。 An acidic protein beverage having a pH of 3.3 to 4.4 containing 0.1 to 0.4% by weight of water-soluble soybean polysaccharide and 0.2 to 0.5% by weight of gum arabic with respect to the beverage, The acidic protein beverage has a B-type rotational viscometer and has a rotor No. An acidic protein beverage that is 5 mPa · s or less when measured at 19, 60 rpm, 1 minute, and 20 ° C., and contains 0.05 to 1.5% by weight of protein.
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