WO2016017832A1 - Nucleic acid amplification device - Google Patents

Nucleic acid amplification device Download PDF

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Publication number
WO2016017832A1
WO2016017832A1 PCT/KR2014/006966 KR2014006966W WO2016017832A1 WO 2016017832 A1 WO2016017832 A1 WO 2016017832A1 KR 2014006966 W KR2014006966 W KR 2014006966W WO 2016017832 A1 WO2016017832 A1 WO 2016017832A1
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nucleic acid
acid amplification
constant temperature
reaction tube
block
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PCT/KR2014/006966
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French (fr)
Korean (ko)
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이현영
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이현영
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Priority to PCT/KR2014/006966 priority Critical patent/WO2016017832A1/en
Publication of WO2016017832A1 publication Critical patent/WO2016017832A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/36—Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
    • C12M1/38—Temperature-responsive control

Definitions

  • the present invention relates to a nucleic acid amplification apparatus, and more particularly, to a nucleic acid amplification apparatus having increased nucleic acid amplification efficiency by alternately contacting a thermoblock having different temperatures to a nucleic acid amplification reagent mixture.
  • nucleic acid amplification technology is a very important technique for molecular gene analysis, and is used as an important technique for analysis of human genome research, detection of infectious and genetic diseases, and the like.
  • the PCR (Polymerase Chain Reaction) method which is a representative technique of the nucleic acid amplification technology, is the most widely known technique, and is a technology capable of selectively amplifying a large amount of a specific nucleic acid sequence only.
  • the general PCR method amplifies a specific sequence in a large amount by repeating the process of denaturation and extension extension. Therefore, in order to repeat this process of denaturation hybridization, the nucleic acid amplification reagents must be changed to reach the respective set temperatures.
  • the most commonly used for the temperature repetition is a Peltier device, and a device for amplifying nucleic acids by heating and cooling the temperature cycle in one block is typically used.
  • the nucleic acid amplification apparatus of the type that repeats the heating and cooling of the block itself has problems such as high precision temperature control technology and time required for raising and lowering the temperature, resulting in complicated equipment structure and increased reaction time.
  • Korean Patent Publication No. 10-2012-0075918 multi-chamber reactor for nucleic acid amplification and analysis.
  • the present invention is to provide a nucleic acid amplification apparatus capable of amplifying nucleic acids more quickly and simply by allowing two incubating blocks to be in contact with the nucleic acid amplification solution.
  • a fixing part 12 formed at the center of the chamber 111 to fix the reaction tube module 20;
  • a high temperature constant temperature block 14 coupled to one side of the moving table 13;
  • a nucleic acid amplification apparatus including a power module 16 capable of moving the movement table 13 to the left and right is provided.
  • the fixing portion 12 is a body portion hole formed in the direction of the chamber 111 from the upper portion of the body portion 11,
  • the reaction tube module 20 The reaction tube module 20,
  • reaction tube units 21 capable of containing a nucleic acid amplification material
  • a frame 22 having a plurality of fixing holes 221 for fixing the plurality of reaction tube units 21, respectively;
  • a nucleic acid amplification apparatus comprising a cover member 23 that can cover the plurality of reaction tube unit 21.
  • the high temperature constant temperature block 14 is formed with a plurality of grooves 141,
  • the groove 141 has an upper side and a side surface of the low temperature constant temperature block direction 15,
  • the high temperature constant temperature block 14 is provided with a nucleic acid amplification apparatus, characterized in that the heating device 142 is coupled.
  • a plurality of grooves 151 are formed in the low temperature constant temperature block 15,
  • the groove 151 has an upper side and a side surface in the high temperature constant temperature block 14 direction,
  • the low temperature constant temperature block 15 is provided with a nucleic acid amplification apparatus, characterized in that the heating device 152 and the cooling device 153 is coupled.
  • the present invention provides a nucleic acid amplification apparatus capable of amplifying nucleic acids more quickly and simply by alternately contacting two thermoblocks (high temperature incubation block and low temperature incubation block) with a nucleic acid amplification solution.
  • FIG. 1 is a perspective view of a nucleic acid amplification apparatus according to an embodiment of the present invention.
  • Figure 2 is a perspective view showing a process of inserting a reaction tube module containing a nucleic acid amplification solution in a nucleic acid amplification apparatus according to an embodiment of the present invention.
  • Figure 3 is a cross-sectional view of the nucleic acid amplification apparatus according to an embodiment of the present invention.
  • FIG. 4 is a perspective view of a drive module according to an embodiment of the present invention.
  • Figure 5 is a cross-sectional view of the drive module of the nucleic acid amplification apparatus according to an embodiment of the present invention (the reaction tube module is in contact with the high temperature constant temperature block).
  • Figure 6 is a cross-sectional view of the drive module of the nucleic acid amplification apparatus according to an embodiment of the present invention (the reaction tube module is in contact with the low temperature constant temperature block).
  • Figure 7 is an exploded perspective view of the reaction tube module according to an embodiment of the present invention.
  • FIG. 8 is a perspective view of a reaction tube module according to an embodiment of the present invention.
  • a nucleic acid amplification apparatus including a power module 16 capable of moving 13) left and right.
  • FIG. 1 is a perspective view of a nucleic acid amplification apparatus according to an embodiment of the present invention
  • Figure 2 is a perspective view showing a process of inserting a reaction tube module containing a nucleic acid amplification solution in a nucleic acid amplification apparatus according to an embodiment of the present invention
  • 3 is a cross-sectional view of a nucleic acid amplification apparatus according to an embodiment of the present invention
  • Figure 4 is a perspective view of a driving module according to an embodiment of the present invention
  • Figure 5 is a nucleic acid amplification apparatus according to an embodiment of the present invention
  • a cross-sectional view of the drive module (the reaction tube module is in contact with the high temperature constant temperature block)
  • Figure 6 is a cross-sectional view of the drive module of the nucleic acid amplification apparatus according to an embodiment of the present invention (reaction tube module is in contact with the low temperature constant temperature block)
  • 7 is an exploded perspective view of a reaction tube module according to an embodiment of the present
  • the body portion 11 is formed with a chamber 111; A fixing part 12 formed at the center of the chamber 111 to fix the reaction tube module 20; a moving table 13 coupled to the inside of the chamber 111; and the moving table 13
  • the high temperature constant temperature block 14 coupled to one side of the; and
  • the low temperature constant temperature block 15 coupled to the other side of the moving table 13, disposed to face the high temperature constant temperature block 14; 13) includes a power module 16 capable of moving left and right.
  • the body portion 11 forms the appearance of the nucleic acid amplification apparatus 10, and various electronic components and electronic devices are embedded therein, and a display device may be additionally coupled to the outside. In addition, the body portion 11 may be coupled to the input module for operating the nucleic acid amplification device (10).
  • the chamber 111 is formed inside the body portion 11.
  • the reaction tube module 20 may be coupled to the inside of the chamber 111.
  • a fixing part 12 to which the reaction tube module 20 is fixed is formed in the center of the chamber 111.
  • the fixing part 12 may be a body hole formed in the body part 11. As the upper portion of the reaction tube module 20 spans the body hole, most of the reaction tube module 20 enters into the chamber 111.
  • the moving table 13 is coupled to the inside of the chamber 111.
  • the moving table 13 is preferably horizontally coupled to the chamber 111.
  • the movement table 13 may reciprocate left and right by the operation of the power module 16 (see FIG. 3).
  • the high temperature constant temperature block 14 is coupled to one side of the upper portion of the moving table 13.
  • the low temperature constant temperature block 15 is coupled to the other side of the upper portion of the moving table 13.
  • the low temperature constant temperature block 15 is disposed to face the high temperature constant temperature block 14.
  • the high temperature constant temperature block 14 is preferably maintained at about 90 degrees Celsius to about 95 degrees Celsius.
  • a plurality of grooves 141 are formed in the high temperature constant temperature block 14, and the groove 141 has an upper side and a side surface of the low temperature constant temperature block 15.
  • the heating device 142 is coupled to the high temperature constant temperature block 14.
  • the heat generator 142 may be a Peltier device.
  • the low temperature constant temperature block 15 is preferably maintained at about 50 degrees Celsius to about 70 degrees Celsius.
  • a plurality of grooves 151 are formed in the low temperature constant temperature block 15, and the groove 151 has an open side and a side in the direction of the high temperature constant temperature block 14, and the heat generating device 152 in the low temperature constant temperature block 15.
  • the cooling device 153 may be combined. By controlling the heating device 152 and the cooling device 153, the low temperature constant temperature block 15 may be maintained at about 50 degrees Celsius to about 70 degrees Celsius.
  • the reaction tube module 20 may have an upper portion fixed to the fixing portion 12.
  • the fixing part 12 may be a body hole formed in the direction of the chamber 111 from the upper part of the body part 11.
  • the reaction tube module 20 includes: a plurality of reaction tube units 21 capable of containing a nucleic acid amplification material; A frame 22 having a plurality of fixing holes 221 for fixing the plurality of reaction tube units 21, respectively; It includes a cover member 23 that can cover a plurality of reaction tube unit 21 (see Fig. 7).
  • the power module 16 is composed of a combination of a motor and a gear, and repeatedly moves the movement table 13 to the left and right by forward rotation of the motor.
  • the moving table 13 may be moved by a combination of a linear gear coupled to the bottom of the movement table 13 and a circular gear gear coupled to a motor.
  • the power module 16 may move the moving table 13 using a chain or a belt.
  • the high temperature constant temperature block 14 is in contact with the reaction tube module 20 to increase the temperature of the nucleic acid amplification material in the reaction tube module 20. Thereafter, after a predetermined time passes, when the power module 16 returns to the counterclockwise direction, as shown in FIG. Combined. As such, while the moving table 13 moves left and right, the reaction tube module 20 is in contact with the high temperature constant temperature block 14 or the low temperature constant temperature block 15. Thus, timely reaction tube module 20 is placed in a high temperature and low temperature environment.
  • the present invention can be utilized in the nucleic acid amplifier industry.

Abstract

A nucleic acid amplification device is disclosed. Provided is a nucleic acid amplification device comprising: a body part (11) having a chamber (111); a fixing part (12) formed in the center of the chamber (111) such that a reaction tube module (20) is fixed thereto; a moving table (13) coupled to the inside of the chamber (111); a constant high temperature block (14) coupled to one side of the moving table (13); a constant low temperature block (15) coupled to the other side of the moving table (13) and arranged so as to be opposite to the constant high temperature block (14); and a power module (16) capable of moving the moving table (13) right and left.

Description

핵산 증폭장치Nucleic Acid Amplification Device
본 발명은 핵산 증폭 장치에 관한 것으로서, 보다 구체적으로는 핵산 증폭시약 혼합물에 온도가 다른 항온블록을 교대로 접촉함으로써 핵산 증폭효율을 높인 핵산 증폭장치에 관한 것이다. The present invention relates to a nucleic acid amplification apparatus, and more particularly, to a nucleic acid amplification apparatus having increased nucleic acid amplification efficiency by alternately contacting a thermoblock having different temperatures to a nucleic acid amplification reagent mixture.
일반적으로 핵산증폭 기술은 분자단위의 유전자 분석에 있어 매우 중요한 기술로서 인간 유전체 연구, 감염성 및 유전적 질병의 검출 등의 분석에 매우 중요한 기술로 활용되고 있다. 이러한 핵산증폭 기술의 대표적인 기술인 PCR(Polymerase Chain Reaction; 중합효소연쇄반응) 방법은 가장 널리 알려진 기술로서, 특정한 핵산서열만을 선택적으로 대량 증폭할 수 있는 기술이다. 일반적인 PCR 방법은 변성(Denaturation) 교잡 (Anealing) 신장 (Extension)의 과정을 반복함으로써 특정한 염기서열을 대량으로 증폭하게 된다. 그러므로 이러한 변성(Denaturation) 교잡 (Anealing) 신장 (Extension)의 과정을 반복하기 위해서는 핵산증폭 시약을 각각의 설정된 온도에 이르도록 변경시켜줘야 한다. 상기한 온도 반복을 위해 가장 일반적으로 사용되는 것이 펠티어(Peltier) 소자를 이용한 것으로서 가열 및 냉각의 온도 순환을 하나의 블록에서 이뤄지도록 함으로써 핵산을 증폭하는 장치가 대표적으로 사용되어지고 있다. 그러나 상기한 블록 자체를 가열 및 냉각을 반복하는 형태의 핵산증폭 장치는 고정밀의 온도제어기술 및 온도를 올리고 내리는데 필요한 시간의 소요됨으로써 장비의 구조가 복잡해지고 반응시간이 늘어나는 등의 문제점이 있다. In general, nucleic acid amplification technology is a very important technique for molecular gene analysis, and is used as an important technique for analysis of human genome research, detection of infectious and genetic diseases, and the like. The PCR (Polymerase Chain Reaction) method, which is a representative technique of the nucleic acid amplification technology, is the most widely known technique, and is a technology capable of selectively amplifying a large amount of a specific nucleic acid sequence only. The general PCR method amplifies a specific sequence in a large amount by repeating the process of denaturation and extension extension. Therefore, in order to repeat this process of denaturation hybridization, the nucleic acid amplification reagents must be changed to reach the respective set temperatures. The most commonly used for the temperature repetition is a Peltier device, and a device for amplifying nucleic acids by heating and cooling the temperature cycle in one block is typically used. However, the nucleic acid amplification apparatus of the type that repeats the heating and cooling of the block itself has problems such as high precision temperature control technology and time required for raising and lowering the temperature, resulting in complicated equipment structure and increased reaction time.
이와 관련된 기술은 한국공개특허 제10-2012-0075918호(핵산증폭 및 분석을 위한 멀티-챔버 반응기)가 개시되어 있다.The related technology is disclosed in Korean Patent Publication No. 10-2012-0075918 (multi-chamber reactor for nucleic acid amplification and analysis).
본 발명은 두 개의 항온블록을 핵산증폭용액에 교대로 접촉되도록 함으로써 보다 신속하고 간편하게 핵산증폭이 가능한 핵산 증폭장치를 제공하고자 한다.The present invention is to provide a nucleic acid amplification apparatus capable of amplifying nucleic acids more quickly and simply by allowing two incubating blocks to be in contact with the nucleic acid amplification solution.
본 발명의 일측면에 따르면,According to one aspect of the invention,
챔버(111)가 형성된 몸체부(11)와;A body portion 11 in which the chamber 111 is formed;
반응튜브모듈(20)이 고정될 수 있도록 상기 챔버(111)의 중심부에 형성되는 고정부(12)와;A fixing part 12 formed at the center of the chamber 111 to fix the reaction tube module 20;
상기 챔버(111)의 내부에 결합된 이동 테이블(13)과;A moving table (13) coupled to the inside of the chamber (111);
상기 이동 테이블(13)의 일측에 결합된 고온 항온블록(14)과;A high temperature constant temperature block 14 coupled to one side of the moving table 13;
상기 이동 테이블(13)의 타측에 결합되며, 상기 고온 항온블록(14)과 마주보도록 배치된 저온 항온블록(15)과;A low temperature constant temperature block (15) coupled to the other side of the moving table (13) and disposed to face the high temperature constant temperature block (14);
상기 이동 테이블(13)을 좌우로 이동시킬 수 있는 동력모듈(16)을 포함하는 핵산 증폭장치가 제공된다.A nucleic acid amplification apparatus including a power module 16 capable of moving the movement table 13 to the left and right is provided.
또한,Also,
상기 고정부(12)는 상기 몸체부(11)의 상부에서 상기 챔버(111) 방향으로 형성된 몸체부 홀이며,The fixing portion 12 is a body portion hole formed in the direction of the chamber 111 from the upper portion of the body portion 11,
상기 반응튜브모듈(20)은,The reaction tube module 20,
핵산증폭물질을 담을 수 있는 복수의 반응튜브유닛(21)과;A plurality of reaction tube units 21 capable of containing a nucleic acid amplification material;
상기 복수의 반응튜브유닛(21)을 각각 고정할 수 있는 고정홀(221)이 복수로 형성된 프레임(22)과;A frame 22 having a plurality of fixing holes 221 for fixing the plurality of reaction tube units 21, respectively;
상기 복수의 반응튜브유닛(21)의 커버할 수 있는 커버부재(23)를 포함하는 것을 특징으로 하는 핵산 증폭장치가 제공된다.Provided is a nucleic acid amplification apparatus comprising a cover member 23 that can cover the plurality of reaction tube unit 21.
또한,Also,
상기 고온 항온블록(14)에는 복수의 홈(141)이 형성되어 있으며,The high temperature constant temperature block 14 is formed with a plurality of grooves 141,
상기 홈(141)은 상부와 상기 저온 항온블록방향(15)의 측면이 개방되어 있으며,The groove 141 has an upper side and a side surface of the low temperature constant temperature block direction 15,
상기 고온 항온블록(14)에는 발열장치(142)가 결합된 것을 특징으로 하는 핵산 증폭장치가 제공된다.The high temperature constant temperature block 14 is provided with a nucleic acid amplification apparatus, characterized in that the heating device 142 is coupled.
또한,Also,
상기 저온 항온블록(15)에는 복수의 홈(151)이 형성되어 있으며,A plurality of grooves 151 are formed in the low temperature constant temperature block 15,
상기 홈(151)은 상부와 상기 고온 항온블록(14) 방향의 측면이 개방되어 있으며,The groove 151 has an upper side and a side surface in the high temperature constant temperature block 14 direction,
상기 저온 항온블록(15)에는 발열장치(152)와 냉각장치(153)가 결합된 것을 특징으로 하는 핵산 증폭장치가 제공된다.The low temperature constant temperature block 15 is provided with a nucleic acid amplification apparatus, characterized in that the heating device 152 and the cooling device 153 is coupled.
본 발명은 두 개의 항온블록(고온 항온블록과 저온 항온블록)을 핵산증폭용액에 교대로 접촉되도록 함으로써 보다 신속하고 간편하게 핵산증폭이 가능한 핵산 증폭장치를 제공한다.The present invention provides a nucleic acid amplification apparatus capable of amplifying nucleic acids more quickly and simply by alternately contacting two thermoblocks (high temperature incubation block and low temperature incubation block) with a nucleic acid amplification solution.
도 1은 본 발명의 일 실시예에 따른 핵산 증폭장치의 사시도.1 is a perspective view of a nucleic acid amplification apparatus according to an embodiment of the present invention.
도 2는 본 발명의 일 실시예에 따른 핵산 증폭장치에 핵산증폭용액이 담긴 반응튜브모듈을 삽입하는 과정을 보여주는 사시도.Figure 2 is a perspective view showing a process of inserting a reaction tube module containing a nucleic acid amplification solution in a nucleic acid amplification apparatus according to an embodiment of the present invention.
도 3은 본 발명의 일 실시예에 따른 핵산 증폭장치의 단면도.Figure 3 is a cross-sectional view of the nucleic acid amplification apparatus according to an embodiment of the present invention.
도 4는 본 발명의 일 실시예에 따른 구동모듈의 사시도.4 is a perspective view of a drive module according to an embodiment of the present invention.
도 5는 본 발명의 일 실시예에 따른 핵산 증폭장치의 구동모듈의 단면도(고온 항온블록에 반응튜브모듈이 접촉된 형태).Figure 5 is a cross-sectional view of the drive module of the nucleic acid amplification apparatus according to an embodiment of the present invention (the reaction tube module is in contact with the high temperature constant temperature block).
도 6은 본 발명의 일 실시예에 따른 핵산 증폭장치의 구동모듈의 단면도(저온 항온블록에 반응튜브모듈이 접촉된 형태).Figure 6 is a cross-sectional view of the drive module of the nucleic acid amplification apparatus according to an embodiment of the present invention (the reaction tube module is in contact with the low temperature constant temperature block).
도 7은 본 발명의 일 실시예에 따른 반응튜브모듈의 분리사시도.Figure 7 is an exploded perspective view of the reaction tube module according to an embodiment of the present invention.
도 8은 본 발명의 일 실시예에 따른 반응튜브모듈의 사시도.8 is a perspective view of a reaction tube module according to an embodiment of the present invention.
챔버(111)가 형성된 몸체부(11)와; 반응튜브모듈(20)이 고정될 수 있도록 상기 챔버(111)의 중심부에 형성되는 고정부(12)와;상기 챔버(111)의 내부에 결합된 이동 테이블(13)과;상기 이동 테이블(13)의 일측에 결합된 고온 항온블록(14)과;상기 이동 테이블(13)의 타측에 결합되며, 상기 고온 항온블록(14)과 마주보도록 배치된 저온 항온블록(15)과;상기 이동 테이블(13)을 좌우로 이동시킬 수 있는 동력모듈(16)을 포함하는 핵산 증폭장치가 제공된다.A body portion 11 in which the chamber 111 is formed; A fixing part 12 formed at the center of the chamber 111 to fix the reaction tube module 20; a moving table 13 coupled to the inside of the chamber 111; and the moving table 13 The high temperature constant temperature block 14 coupled to one side of the; and The low temperature constant temperature block 15 coupled to the other side of the moving table 13, disposed to face the high temperature constant temperature block 14; Provided is a nucleic acid amplification apparatus including a power module 16 capable of moving 13) left and right.
이하에는, 본 발명의 바람직한 실시예를 첨부도면을 참조로 상세하게 설명하되, 이는 본 발명에 속하는 기술분야에서 통상의 지식을 가진 자가 본 발명을 용이하게 실시할 수 있을 정도로 상세하게 설명하기 위한 것이지, 이로써 본 발명의 사상 및 범주가 한정되는 것을 의미하지는 않는다.DETAILED DESCRIPTION Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings, which are intended to explain in detail enough to enable those skilled in the art to easily carry out the present invention. This does not mean that the spirit and scope of the present invention is limited.
도 1은 본 발명의 일 실시예에 따른 핵산 증폭장치의 사시도이며, 도 2는 본 발명의 일 실시예에 따른 핵산 증폭장치에 핵산증폭용액이 담긴 반응튜브모듈을 삽입하는 과정을 보여주는 사시도이며, 도 3은 본 발명의 일 실시예에 따른 핵산 증폭장치의 단면도이며, 도 4는 본 발명의 일 실시예에 따른 구동모듈의 사시도이며,도 5는 본 발명의 일 실시예에 따른 핵산 증폭장치의 구동모듈의 단면도(고온 항온블록에 반응튜브모듈이 접촉된 형태)이며, 도 6은 본 발명의 일 실시예에 따른 핵산 증폭장치의 구동모듈의 단면도(저온 항온블록에 반응튜브모듈이 접촉된 형태)이며, 도 7은 본 발명의 일 실시예에 따른 반응튜브모듈의 분리사시도이며, 도 8은 본 발명의 일 실시예에 따른 반응튜브모듈의 사시도이다.1 is a perspective view of a nucleic acid amplification apparatus according to an embodiment of the present invention, Figure 2 is a perspective view showing a process of inserting a reaction tube module containing a nucleic acid amplification solution in a nucleic acid amplification apparatus according to an embodiment of the present invention, 3 is a cross-sectional view of a nucleic acid amplification apparatus according to an embodiment of the present invention, Figure 4 is a perspective view of a driving module according to an embodiment of the present invention, Figure 5 is a nucleic acid amplification apparatus according to an embodiment of the present invention A cross-sectional view of the drive module (the reaction tube module is in contact with the high temperature constant temperature block), Figure 6 is a cross-sectional view of the drive module of the nucleic acid amplification apparatus according to an embodiment of the present invention (reaction tube module is in contact with the low temperature constant temperature block) 7 is an exploded perspective view of a reaction tube module according to an embodiment of the present invention, and FIG. 8 is a perspective view of the reaction tube module according to an embodiment of the present invention.
본 실시예의 핵산 증폭장치(10)는,챔버(111)가 형성된 몸체부(11)와; 반응튜브모듈(20)이 고정될 수 있도록 상기 챔버(111)의 중심부에 형성되는 고정부(12)와;상기 챔버(111)의 내부에 결합된 이동 테이블(13)과;상기 이동 테이블(13)의 일측에 결합된 고온 항온블록(14)과;상기 이동 테이블(13)의 타측에 결합되며, 상기 고온 항온블록(14)과 마주보도록 배치된 저온 항온블록(15)과;상기 이동 테이블(13)을 좌우로 이동시킬 수 있는 동력모듈(16)을 포함한다.Nucleic acid amplification apparatus 10 of the present embodiment, the body portion 11 is formed with a chamber 111; A fixing part 12 formed at the center of the chamber 111 to fix the reaction tube module 20; a moving table 13 coupled to the inside of the chamber 111; and the moving table 13 The high temperature constant temperature block 14 coupled to one side of the; and The low temperature constant temperature block 15 coupled to the other side of the moving table 13, disposed to face the high temperature constant temperature block 14; 13) includes a power module 16 capable of moving left and right.
몸체부(11)는 핵산 증폭장치(10)의 외관을 형성하는 것으로서, 각종 전자부품 및 전자소자 등이 내장되어 있으며, 외부에는 디스플레이 장치가 추가적으로 결합될 수 있다. 아울러 몸체부(11)에는 핵산 증폭장치(10)를 작동시키기 위한 입력모듈이 결합될 수 있다.The body portion 11 forms the appearance of the nucleic acid amplification apparatus 10, and various electronic components and electronic devices are embedded therein, and a display device may be additionally coupled to the outside. In addition, the body portion 11 may be coupled to the input module for operating the nucleic acid amplification device (10).
몸체부(11) 내부에는 챔버(111)가 형성되어 있다. 챔버(111)의 내부에 반응튜브모듈(20)이 결합될 수 있다. 챔버(111)의 중심부에는 반응튜브모듈(20)이 고정될 수 있는 고정부(12)가 형성되어 있다. 고정부(12)는 몸체부(11)에 형성된 몸체부 홀일 수 있다. 몸체부 홀에 반응튜브모듈(20)의 상부가 걸쳐짐으로써, 반응튜브모듈(20)의 대부분은 챔버(111) 내부로 들어가게 된다. The chamber 111 is formed inside the body portion 11. The reaction tube module 20 may be coupled to the inside of the chamber 111. In the center of the chamber 111, a fixing part 12 to which the reaction tube module 20 is fixed is formed. The fixing part 12 may be a body hole formed in the body part 11. As the upper portion of the reaction tube module 20 spans the body hole, most of the reaction tube module 20 enters into the chamber 111.
챔버(111)의 내부에는 이동 테이블(13)이 결합되어 있다. 이동 테이블(13)은 챔버(111)에 수평으로 결합되는 것이 좋다. 이동 테이블(13)은 동력모듈(16)의 작동으로 좌우로 왕복운동할 수 있다.(도 3 참조)The moving table 13 is coupled to the inside of the chamber 111. The moving table 13 is preferably horizontally coupled to the chamber 111. The movement table 13 may reciprocate left and right by the operation of the power module 16 (see FIG. 3).
이동 테이블(13)의 상부의 일측에는 고온 항온블록(14)이 결합된다. 이동 테이블(13)의 상부의 타측에는 저온 항온블록(15)이 결합된다. 저온 항온블록(15)은 고온 항온블록(14)과 마주보도록 배치된다.The high temperature constant temperature block 14 is coupled to one side of the upper portion of the moving table 13. The low temperature constant temperature block 15 is coupled to the other side of the upper portion of the moving table 13. The low temperature constant temperature block 15 is disposed to face the high temperature constant temperature block 14.
고온 항온블록(14)은 섭씨90도에서 섭씨 95도 정도로 유지되는 것이 좋다. 고온 항온블록(14)에는 복수의 홈(141)이 형성되어 있으며, 홈(141)은 상부와 저온 항온블록(15)의 측면이 개방되어 있다. 고온 항온블록(14)에는 발열장치(142)가 결합된다. 발열장치(142)는 펠티어(Peltier)소자일 수 있다.The high temperature constant temperature block 14 is preferably maintained at about 90 degrees Celsius to about 95 degrees Celsius. A plurality of grooves 141 are formed in the high temperature constant temperature block 14, and the groove 141 has an upper side and a side surface of the low temperature constant temperature block 15. The heating device 142 is coupled to the high temperature constant temperature block 14. The heat generator 142 may be a Peltier device.
저온 항온블록(15)은 섭씨 50도에서 섭씨70도 정도로 유지되는 것이 좋다. 저온 항온블록(15)에는 복수의 홈(151)이 형성되어 있으며, 홈(151)은 상부와 고온 항온블록(14) 방향의 측면이 개방되어 있으며, 저온 항온블록(15)에는 발열장치(152)와 냉각장치(153)가 결합될 수 있다. 발열장치(152)와 냉각장치(153)의 제어에 의해서 저온 항온블록(15)은 섭씨 50도에서 섭씨70도 정도로 유지될 수 있다.The low temperature constant temperature block 15 is preferably maintained at about 50 degrees Celsius to about 70 degrees Celsius. A plurality of grooves 151 are formed in the low temperature constant temperature block 15, and the groove 151 has an open side and a side in the direction of the high temperature constant temperature block 14, and the heat generating device 152 in the low temperature constant temperature block 15. ) And the cooling device 153 may be combined. By controlling the heating device 152 and the cooling device 153, the low temperature constant temperature block 15 may be maintained at about 50 degrees Celsius to about 70 degrees Celsius.
반응튜브모듈(20)은 고정부(12)에 상부가 고정될 수 있다. 고정부(12)는 상기 몸체부(11)의 상부에서 챔버(111) 방향으로 형성된 몸체부 홀일 수 있다.The reaction tube module 20 may have an upper portion fixed to the fixing portion 12. The fixing part 12 may be a body hole formed in the direction of the chamber 111 from the upper part of the body part 11.
반응튜브모듈(20)은,핵산증폭물질을 담을 수 있는 복수의 반응튜브유닛(21)과; 복수의 반응튜브유닛(21)을 각각 고정할 수 있는 고정홀(221)이 복수로 형성된 프레임(22)과; 복수의 반응튜브유닛(21)의 커버할 수 있는 커버부재(23)를 포함한다.(도 7 참조)The reaction tube module 20 includes: a plurality of reaction tube units 21 capable of containing a nucleic acid amplification material; A frame 22 having a plurality of fixing holes 221 for fixing the plurality of reaction tube units 21, respectively; It includes a cover member 23 that can cover a plurality of reaction tube unit 21 (see Fig. 7).
동력모듈(16)은 모터와 기어의 조합으로 이루어져 있으며, 모터의 정회전 역회전으로 이동 테이블(13)을 좌우로 반복적으로 이동시킨다. 이동 테이블(13)을 이동시키는 방법은 이동 테이블(13) 하부에 결합된 리니어기어와 모터와 결합된 원형 톱니기어의 조합으로 이루어질 수도 있다. 동력모듈(16)은 체인이나 벨트를 이용하여 이동 테이블(13)을 이동시킬 수도 있다.The power module 16 is composed of a combination of a motor and a gear, and repeatedly moves the movement table 13 to the left and right by forward rotation of the motor. The moving table 13 may be moved by a combination of a linear gear coupled to the bottom of the movement table 13 and a circular gear gear coupled to a motor. The power module 16 may move the moving table 13 using a chain or a belt.
도 5의 경우, 고온 항온블록(14)이 반응튜브모듈(20)과 접촉하여 반응튜브모듈(20)내의 핵산증폭물질의 온도를 높인다. 이후, 소정의 시간이 흐른 후, 동력모듈(16)이 시계반대방향으로 돌아가면, 도 6과 같이 이동 테이블(13)이 좌측으로 이동하여 저온 항온블록(15)이 반응튜브모듈(20)과 결합된다. 이와 같이, 이동 테이블(13)이 좌우로 움직이면서, 반응튜브모듈(20)은 고온 항온블록(14)과 접촉하거나 저온 항온블록(15)에 접촉한다. 따라서, 적시에 반응튜브모듈(20)은 고온과 저온 환경에 놓이게 된다.In the case of Figure 5, the high temperature constant temperature block 14 is in contact with the reaction tube module 20 to increase the temperature of the nucleic acid amplification material in the reaction tube module 20. Thereafter, after a predetermined time passes, when the power module 16 returns to the counterclockwise direction, as shown in FIG. Combined. As such, while the moving table 13 moves left and right, the reaction tube module 20 is in contact with the high temperature constant temperature block 14 or the low temperature constant temperature block 15. Thus, timely reaction tube module 20 is placed in a high temperature and low temperature environment.
이상에서 본 발명의 실시예에 대해서 상세히 설명하였으나, 이는 하나의 실시예에 불과하며, 이로써 본 발명의 특허청구범위를 한정하는 것은 아니다. 본 실시예를 바탕으로 균등한 범위까지 당업자가 변형 및 추가하는 범위도 본 발명의 권리범위에 속한다 할 것이다. Although the embodiment of the present invention has been described in detail above, this is only one embodiment, and thus, does not limit the claims of the present invention. Based on the present embodiment, modifications and additions made by those skilled in the art to the equivalent range will also belong to the scope of the present invention.
본 발명은 핵산증폭장치 산업분야에 활용될 수 있다.The present invention can be utilized in the nucleic acid amplifier industry.

Claims (4)

  1. 챔버(111)가 형성된 몸체부(11)와; A body portion 11 in which the chamber 111 is formed;
    반응튜브모듈(20)이 고정될 수 있도록 상기 챔버(111)의 중심부에 형성되는 고정부(12)와;A fixing part 12 formed at the center of the chamber 111 to fix the reaction tube module 20;
    상기 챔버(111)의 내부에 결합된 이동 테이블(13)과;A moving table (13) coupled to the inside of the chamber (111);
    상기 이동 테이블(13)의 일측에 결합된 고온 항온블록(14)과;A high temperature constant temperature block 14 coupled to one side of the moving table 13;
    상기 이동 테이블(13)의 타측에 결합되며, 상기 고온 항온블록(14)과 마주보도록 배치된 저온 항온블록(15)과;A low temperature constant temperature block (15) coupled to the other side of the moving table (13) and disposed to face the high temperature constant temperature block (14);
    상기 이동 테이블(13)을 좌우로 이동시킬 수 있는 동력모듈(16)을 포함하는 핵산 증폭장치.Nucleic acid amplification apparatus comprising a power module (16) capable of moving the movement table (13) from side to side.
  2. 제1항에 있어서,The method of claim 1,
    상기 고정부(12)는 상기 몸체부(11)의 상부에서 상기 챔버(111) 방향으로 형성된 몸체부 홀이며,The fixing portion 12 is a body portion hole formed in the direction of the chamber 111 from the upper portion of the body portion 11,
    상기 반응튜브모듈(20)은,The reaction tube module 20,
    핵산증폭물질을 담을 수 있는 복수의 반응튜브유닛(21)과;A plurality of reaction tube units 21 capable of containing a nucleic acid amplification material;
    상기 복수의 반응튜브유닛(21)을 각각 고정할 수 있는 고정홀(221)이 복수로 형성된 프레임(22)과;A frame 22 having a plurality of fixing holes 221 for fixing the plurality of reaction tube units 21, respectively;
    상기 복수의 반응튜브유닛(21)의 커버할 수 있는 커버부재(23)를 포함하는 것을 특징으로 하는 핵산 증폭장치.A nucleic acid amplification apparatus comprising a cover member 23 that can cover the plurality of reaction tube unit (21).
  3. 제2항에 있어서,The method of claim 2,
    상기 고온 항온블록(14)에는 복수의 홈(141)이 형성되어 있으며,The high temperature constant temperature block 14 is formed with a plurality of grooves 141,
    상기 홈(141)은 상부와 상기 저온 항온블록(15) 방향의 측면이 개방되어 있으며,The groove 141 has an upper side and a side in the direction of the low temperature constant temperature block 15 is opened,
    상기 고온 항온블록(14)에는 발열장치(142)가 결합된 것을 특징으로 하는 핵산 증폭장치. The high temperature constant temperature block 14 is a nucleic acid amplification apparatus, characterized in that the heating device 142 is coupled.
  4. 제3항에 있어서,The method of claim 3,
    상기 저온 항온블록(15)에는 복수의 홈(151)이 형성되어 있으며,A plurality of grooves 151 are formed in the low temperature constant temperature block 15,
    상기 홈(151)은 상부와 상기 고온 항온블록(14) 방향의 측면이 개방되어 있으며,The groove 151 has an upper side and a side surface in the high temperature constant temperature block 14 direction,
    상기 저온 항온블록(15)에는 발열장치(152)와 냉각장치(153)가 결합된 것을 특징으로 하는 핵산 증폭장치.The low temperature constant temperature block 15 is a nucleic acid amplification device, characterized in that the heating device 152 and the cooling device 153 is combined.
PCT/KR2014/006966 2014-07-30 2014-07-30 Nucleic acid amplification device WO2016017832A1 (en)

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CN110872557A (en) * 2018-08-30 2020-03-10 深圳市刚竹医疗科技有限公司 PCR centrifugal microfluidic device and method thereof

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