WO2017071339A1

WO2017071339A1 - Plunger pump dispensing and staining assembly, and staining machine

Info

Publication number: WO2017071339A1
Application number: PCT/CN2016/091870
Authority: WO
Inventors: 李必松; 朱汉超; 罗琪; 陈恒; 梁万兴
Original assignee: 广州鸿琪光学仪器科技有限公司
Priority date: 2015-10-30
Filing date: 2016-07-27
Publication date: 2017-05-04
Also published as: CN105259008A

Abstract

A plunger pump dispensing and staining assembly comprises a staining container (1), a buffer solution container (2), staining probes (3), dispenser probes (4), a waste solution removal device, a first plunger pump (6), a second plunger pump (7), a first connector (8), several first two-position three-way solenoid valves (9), and several second two-position three-way solenoid valves (10). The first plunger pump (6) is connected to a common port of each of the first two-position three-way solenoid valves (9), normally close ports of respective first two-position three-way solenoid valves (9) are connected to the staining probes (3) in one-to-one correspondence, and the staining container (1) is connected to normally open ports of respective first two-position three-way solenoid valves (9). The second plunger pump (7) is connected to common ports of the second two-position three-way solenoid valves (10), normally close ports of the second two-position three-way solenoid valves (10) are connected to the dispenser probes (4), and the buffer solution container (2) is connected to normally open ports of the second two-position three-way solenoid valves (10). The waste solution removal device is configured to remove a staining solution and the buffer solution. The staining assembly and a staining machine having the same can simplify the structure, and prevent remaining droplets or leakage.

Description

Piston pump sample dyeing assembly and dyeing machine

Technical field

The invention relates to a cytopathological detection technology, in particular to a plunger pump sample dyeing assembly and a dyeing machine (not hindered here).

Background technique

Liquid-Based Cytology Test ("LCT") technology is mainly used for cervical cytology, especially for cervical cancer screening.

The working principle is as follows: the cervical exfoliated cells are scraped off with a special material picker and completely eluted into a plastic bottle containing the cell preservation liquid, and sent to the laboratory, and the cell mass is depolymerized after being shaken by the shaking stick, and the cell specimen is preserved. The liquid is precipitated by centrifugation, and the cervical epithelial cells are separated from the cell debris, blood, mucus and white blood cells in the specimen to obtain pure cervical epithelial cells, and the cervical epithelial cells are transferred to the glass slide by cell transfer technology. A thin layer of cells of 13 mm in diameter was washed and stained. These processes are all automatically controlled by the system, which can be called to complete under the work of the dyeing machine. Finally, the technicians read the film and read it under the microscope and make a diagnosis according to TBS.

The advantage is that the cell layer made by LCT technology is distributed in a single layer, the background is clear, easy to observe, and the rate of missed detection is low.

The foreign LCT technology is mainly based on AutoCyto (produced by American BD company). Although the quality of the test equipment is good, the price is high, the inspection cost is expensive, the maintenance is not timely, and it is not suitable for mass promotion in China. Recently, some domestic companies have also developed liquid-based cell thin-layer tablet dyeing machines suitable for their national conditions, such as the LCT testing equipment of Guangzhou Anpingping Company (Application No. 2007100272504, a tablet dyeing machine and a method for tableting and dyeing) ) but from According to the results of clinical tests, there are still great differences compared with foreign LCT technologies, and the quality of production is quite different.

The instruments and reagents produced will have a certain influence on the production effect. For the automatic dyeing machine, the cells that have been dyed are mainly caused by the dropping and leakage, and the cells are washed and dyed by other dyes. The dyed part is not dyed, which is the so-called yin and yang film. In addition, if an instrument failure occurs during the production process, all the production will be interrupted, resulting in the failure of the production. When using a syringe to load, the syringe is broken, the pipeline is blocked or leaking (more accessories, more interfaces) is one of the main causes of instrument failure. The dripping and leakage are mainly caused by the sample loading process or by the motor drive mode. In the past, the peristaltic pump was applied, because the catheter of the peristaltic pump was squeezed for a long time, the catheter was easily aging, the peristaltic pump could not be completely closed, and the liquid at the needle tip was dripped; and the syringe was used to load, and the temperature difference changed to affect the sealing performance. Causes leakage. The previous uniform acceleration driving method is mainly composed of a myriad of small acceleration processes. In fact, during the movement, especially when accelerating or decelerating, due to the effect of inertia, it will cause large jitter and will be loaded. The liquid remaining in the needle is shaken off.

In summary, the current LCT technology is relatively mature. The automatic dyeing machine on the market uses syringes for sample loading. The materials of the syringes are all made of glass. When designing the products, the syringes are exposed on the outside of the instrument case. The glass is fragile, so During the production, transportation and use of the product, the syringe is easily damaged due to collision, large temperature difference, etc. The instrument is loaded with a syringe, and various accessories must be assembled to meet the requirements of the instrument operation program, which will increase the weight and space of the instrument and cause The assembly process is cumbersome; there have been devices for peristaltic pumping on the market, but because the catheter is squeezed for a long time, the catheter is prone to aging, shortening the service life of the product, and also causing hanging liquid or dripping liquid.

Summary of the invention

In order to overcome the deficiencies of the prior art, it is an object of the present invention to provide a plunger pump sample dyeing assembly that simplifies the structure and avoids hang-up or liquid leakage.

Another object of the present invention is to provide a dyeing machine using the plunger pump to load a dyeing assembly.

One of the objects of the present invention is achieved by the following technical solutions:

Piston pump sample dyeing assembly, including dyeing bottle, buffer bottle, dyeing needle, sample needle, waste liquid extraction device, first plunger pump, second plunger pump, first connector, several first two a three-way solenoid valve and a second two-position three-way solenoid valve, the first plunger pump is respectively connected to the common end of each of the first two-position three-way solenoid valves through the first connector, and the first two-position three-way solenoid valves are often The closed ends are connected to the dyeing needles one by one, and the dyeing bottles are respectively connected to the normally open ends of the first two-position three-way solenoid valves; the second plunger pump is connected to the common end of the second two-way three-way solenoid valves, and the second two-way three-way The normally closed end of the solenoid valve is connected to the sample needle, and the normally open end of the second two-way three-way solenoid valve is connected to the buffer bottle; the waste liquid extraction device is used for extracting the dye solution and the buffer solution.

Further, the waste liquid extracting device comprises a plurality of waste liquid needles, a second connecting head and a waste liquid pump, and one end of each waste liquid needle is connected to the waste liquid pump through the second connecting head.

The second object of the present invention is achieved by the following technical solutions:

a dyeing machine using the plunger pump to load a dyeing assembly, comprising a table and a working plate both mounted on the table for directly facing the dyeing needle, a sample holder for facing the sample needle, and a nozzle holder and a four-axis linkage mechanism opposite to the sample needle, the four-axis linkage mechanism has an X-axis slide mechanism that slides along the X-axis direction of the table, is mounted on the X-axis slide mechanism, and is along the Y-axis direction of the table Sliding Y-axis slip mechanism, mounted on Y-axis slip mechanism and working along a Z1 axis slip mechanism that slides in the Z-axis direction and a Z2 axis slip mechanism that is mounted on the Y-axis slip mechanism and slidably fits along the Z-axis of the table. The sample needle is mounted on the Z1 axis slip mechanism, and the dyeing needle Installed on the Z2 axis slip mechanism.

Further, the X-axis slip mechanism includes an X-axis slide rail and an X-axis drive mechanism, the Y-axis slide mechanism includes a Y-axis slide rail and a Y-axis drive mechanism, and the Z1-axis slide mechanism includes a Z1 shaft bracket and a Z1-axis drive mechanism. The Z2 axis sliding mechanism includes a Z2 shaft bracket and a Z2 shaft driving mechanism, and the Y-axis sliding rail and the X-axis sliding rail are slidingly matched, and the Z1 shaft bracket and the Z2 shaft bracket are respectively slidably engaged with the Y-axis sliding rail, and the loading needle is mounted on the Z1 shaft. The stent and the dye needle are mounted on the Z2 shaft bracket.

Further, a waste box for storing the nozzle waste and a waste tank for washing the waste liquid are installed on the work table.

Compared with the prior art, the beneficial effects of the present invention are:

The first plunger pump and the second plunger pump of the invention have an integrated structure, which effectively solves problems such as damage caused by collision, large temperature difference, etc. during production, transportation and use, and reduces assembly space and processes, Installation and maintenance are more convenient. At the same time, combined with the on-off control of the first two-position three-way solenoid valve and the second two-way three-way solenoid valve, there is no phenomenon of hanging liquid or dripping liquid.

DRAWINGS

1 is a schematic view showing a dyeing liquid path of a plunger pump sample dyeing assembly of the present invention;

2 is a schematic view of a sample liquid path of a plunger pump sample dyeing assembly of the present invention;

3 is a schematic view showing a waste liquid recovery liquid path of a plunger pump sample dyeing assembly of the present invention;

Figure 4 is a front view of the dyeing machine of the present invention;

Figure 5 is a plan view of Figure 4;

Figure 6 is a left side view of Figure 4.

In the figure: 1, dyeing bottle; 2, buffer bottle; 3, dye needle; 4, needle; 5, waste needle; 6, first plunger pump; 7, second plunger pump; a connector; 9, a first two-position three-way solenoid valve; 10, a second two-way three-way solenoid valve; 11, a waste box; 12, a second connector; 13, a waste pump; 14, a table; , work plate; 16, sample holder; 17, nozzle holder; 18, four-axis linkage mechanism; 181, X-axis slip mechanism; 182, Y-axis slip mechanism; 183, Z1 axis slip mechanism; 184, Z2 axis Slip mechanism.

detailed description

The present invention will be further described below in conjunction with the drawings and specific embodiments.

The plunger pump sample dyeing assembly shown in FIGS. 1 to 3 includes a dyeing bottle 1, a buffer bottle 2, a dyeing needle 3, a sample adding needle 4, a waste liquid extracting device, a first plunger pump 6, and a second column. a plug pump 7, a first connector 8, a plurality of first two-position three-way solenoid valves 9 and a second two-position three-way solenoid valve 10, the first plunger pump 6 is connected to each of the first two bits through the first connector 8 a common end of the three-way solenoid valve 9, the normally closed ends of the first two-position three-way solenoid valves 9 are connected to the dyeing needles one by one, and the dyeing bottles 1 are respectively connected to the normally open ends of the respective first two-position three-way solenoid valves 9; The second plunger pump 7 is connected to the common end of the second two-position three-way solenoid valve 10, and the normally closed end of the second two-position three-way solenoid valve 10 is connected to the sample needle 4, and the second two-position three-way solenoid valve 10 is often The buffer is connected to the buffer bottle 2; the waste liquid extraction device is used for extracting the staining solution and the buffer.

The first plunger pump 6 and the second plunger pump 7 are integrated structures, which effectively solve problems such as damage caused by collision, large temperature difference, etc. during production, transportation and use, and reduce assembly space and process, and installation. More convenient for maintenance, at the same time, combined with the first two-way three-way The on-off control of the solenoid valve 9, the second two-position three-way solenoid valve 10, does not cause the phenomenon of hanging liquid or dripping liquid. The staining solution contained in the dyeing bottle 1 may be one of a buffer solution, a rinse solution, hematoxylin, and EA/OG, and the buffer bottle 2 is used to store a buffer. By energizing and controlling the first two-position three-way solenoid valve 9 and the second two-position three-way solenoid valve 10, the dye solution can be stably and effectively sucked, buffered into the corresponding dyeing needle 3 and the needle 4, and the dye needle 3 Corresponding to the four kinds of staining solutions, such as buffer, rinse, hematoxylin and EA/OG, and the needle 4 is also connected to the buffer, that is, the buffer is connected to the dyeing needle 3 and the needle 4 . In order to group the dyeing needles 3 to balance the liquid injection, the two first two-position three-way solenoid valves 9 can be divided into one group, and correspondingly, the first joint head 8 can be set as the first three-way joint.

Specifically, during operation, the second plunger pump 7 has its own motor rotating forward, and the second two-position three-way solenoid valve 10 is not energized to suck the buffer into the plunger pump; then, the second plunger pump 7 is controlled. The motor is reversed with the motor, and the second two-way three-way solenoid valve 10 is controlled to be energized to inject the buffer into the needle 4. The working principle of the liquid injection of the dyeing liquid is the same as that of the liquid filling of the buffer, and will not be described here.

In order to more conveniently extract the waste liquid, the waste liquid extraction device includes a plurality of waste liquid needles 5, a second joint head 12 and a waste liquid pump 13, and one end of each waste liquid needle 5 is connected to the waste liquid pump through the second joint 12 13. The second connector 12 can be configured as a second three-way connector to evenly distribute the liquid supply.

As shown in FIGS. 4-6, the dyeing machine using the plunger pump loading and dyeing assembly comprises a table 14 and a working disk 15 respectively mounted on the table 14 for facing the dyeing needle 3, for a sample holder 16 facing the sample needle 4, a nozzle holder 17 facing the sample needle 4, and a four-axis linkage mechanism 18 having an X-axis along the table 14 The X-axis slip mechanism 181 that slides in the direction, the Y-axis slip mechanism 182 that is attached to the X-axis slip mechanism 181 and slides along the Y-axis direction of the table 14, is mounted on the Y-axis slip mechanism 182, and is along the table. a Z1-axis slip mechanism 183 that slides in the Z-axis direction of 14 and a Z2-axis slip mechanism 184 that is attached to the Y-axis slip mechanism 182 and slidably fitted in the Z-axis direction of the table 14, and the sample pin 4 is mounted on the Z1 axis. The slip mechanism 183, the dye needle 3 is mounted on the Z2 axis slip mechanism 184; thus, the needle 4 can be moved along the X, Y, and Z1 axes, and the dye needle 3 can be moved along the X, Y, and Z2 axes. The waste liquid extraction device acts on the waste liquid recovery inside the machine pipe, and the waste liquid is recovered by the waste liquid pump installed on the work table and the waste liquid needle installed in the Z1 axis slip mechanism and the Z2 axis slip mechanism. .

The sampling needle 4 sucks the nozzle and then enters the sample holder 16 for aspirating. The four-axis linkage mechanism 18 provides three-axis flexible movement capability for the dyeing needle 3 and the loading needle 4, respectively, to accurately align the working plate 15, respectively. The nozzle holder 17 and the sample holder 16. It can be combined with the control device to achieve a more fully automatic production operation.

Illustratively, the X-axis slip mechanism 181 includes an X-axis slide rail and an X-axis drive mechanism, the Y-axis slide mechanism 182 includes a Y-axis slide rail and a Y-axis drive mechanism, and the Z1-axis slide mechanism 183 includes a Z1-axis bracket and a Z1 bracket. The shaft drive mechanism, the Z2 shaft slip mechanism 184 includes a Z2 shaft bracket and a Z2 shaft drive mechanism, the Y-axis slide rail is slidably engaged with the X-axis slide rail, and the Z1 shaft bracket and the Z2 shaft bracket are respectively slidably engaged with the Y-axis slide rail, and the sample is loaded. The needle 4 is mounted on the Z1 shaft bracket, and the dye needle 3 is mounted on the Z2 shaft bracket. Among them, the X-axis drive mechanism, the Y-axis drive mechanism, the Z1-axis drive mechanism, and the Z2-axis drive mechanism can all be realized by a belt-type transmission mechanism combined with a motor. Of course, couplings, sliders, photoelectric switches and the like can be added to enhance The precision of slipping.

In order to facilitate the recovery of the waste liquid, the work table 14 is also provided with a waste box 11 to which the waste material for storing the nozzles is installed, and a waste liquid tank for flushing the waste liquid. . The waste box 11 is for receiving the sampled waste liquid and the used nozzle waste, and the washing process of the dyeing liquid is completed in the waste liquid tank.

Various other changes and modifications may be made by those skilled in the art in light of the above-described technical solutions and concepts, and all such changes and modifications are intended to fall within the scope of the appended claims.

Claims

The plunger pump sample dyeing assembly is characterized in that it comprises a dyeing bottle, a buffer bottle, a dyeing needle, a sample adding needle, a waste liquid extracting device, a first plunger pump, a second plunger pump, a first connecting head, and a plurality of a first two-position three-way solenoid valve and a second two-position three-way solenoid valve, wherein the first plunger pump is respectively connected to a common end of each of the first two-position three-way solenoid valves through the first connecting head, and each of the first two-position three-way The normally closed ends of the solenoid valves are connected to the dyeing needles one by one, and the dyeing bottles are respectively connected to the normally open ends of the first two-position three-way solenoid valves; the second plunger pump is connected to the common end of the second two-way three-way solenoid valves, second The normally closed end of the two-way three-way solenoid valve is connected to the sample needle, and the normally open end of the second two-way solenoid valve is connected to the buffer bottle; the waste liquid extraction device is used for extracting the dye solution and the buffer solution.
The plunger pump loading and dyeing assembly according to claim 1, wherein the waste liquid extracting device comprises a plurality of waste liquid needles, a second connecting head and a waste liquid pump, and one end of each waste liquid needle passes through the second connecting head. Connect to the waste pump.
A dyeing machine using the plunger pump loading and dyeing assembly according to claim 1 or 2, comprising a work table and a work plate both mounted on the table for directly facing the dyeing needle, for loading and loading The sample holder with the needle facing the right, the nozzle holder for the opposite side of the sample needle and the four-axis linkage mechanism, the four-axis linkage mechanism has an X-axis sliding mechanism that slides along the X-axis direction of the table, and is mounted on the X-axis slide a Y-axis slip mechanism that moves the mechanism and slides along the Y-axis direction of the table, a Z1-axis slip mechanism that is mounted on the Y-axis slip mechanism and slides along the Z-axis direction of the table, and is mounted along the Y-axis slip mechanism The Z2 axis sliding mechanism is slidably engaged in the Z-axis direction of the table, the loading needle is mounted on the Z1 axis sliding mechanism, and the dyeing needle is mounted on the Z2 axis sliding mechanism.
A dyeing machine according to claim 3, wherein the X-axis slip mechanism The utility model comprises an X-axis slide rail and an X-axis drive mechanism, the Y-axis slide mechanism comprises a Y-axis slide rail and a Y-axis drive mechanism, the Z1 shaft slip mechanism comprises a Z1 shaft bracket and a Z1 shaft drive mechanism, and the Z2 axis slip mechanism comprises a Z2 shaft Bracket and Z2 shaft drive mechanism, Y-axis slide rail and S-axis slide rail slide fit, Z1 shaft bracket and Z2 shaft bracket slide and fit with Y-axis slide rail respectively, the sample needle is mounted on the Z1 shaft bracket, and the dye needle is mounted on the Z2 shaft support.
The dyeing machine according to claim 3, characterized in that the work table is further provided with a waste box for storing the nozzle waste and a waste liquid tank for flushing the waste liquid.