A pressure-driven microfluidic device for separating chemical or biological species from a sample provides on-column sample injection from a sample loading segment, with mobile phase solvent supplied to both the separation column and the sample loading segment to promote high-quality separation. Multiple...http://www.google.fr/patents/US20030223913?utm_source=gb-gplus-shareBrevet US20030223913 - Microfluidic separation devices and methods