A method for the stepwise creation of phosphodiester bonds between desired nucleosides resulting in the synthesis of polynucleotides having a predetermined nucleotide sequence by preparing an initiation substrate containing a free and unmodified 3'-hydroxyl group; attaching a mononucleotide selected...http://www.google.fr/patents/US6214987?utm_source=gb-gplus-shareBrevet US6214987 - Compositions for enzyme catalyzed template-independent formation of phosphodiester bonds using protected nucleotides
Compositions for enzyme catalyzed template-independent formation of ...
