The present invention provides a method of assaying an enzyme-mediated coupling reaction between a first and a second reactant. The method includes contacting the first reactant with the second reactant in the presence of the enzyme. The second reactant includes a thiol derivative to yield a first product...http://www.google.fr/patents/US6498005?utm_source=gb-gplus-shareBrevet US6498005 - Homogeneous assay methods