Methods are provided for nucleic acid analysis wherein a target nucleic acid that is at least partially double stranded is mixed with a dsDNA binding dye having a percent saturation of at least 50% to form a mixture. In one embodiment, the nucleic acid is amplified in the presence of the dsDNA binding...http://www.google.fr/patents/US7803551?utm_source=gb-gplus-shareBrevet US7803551 - AMPLICON melting analysis with saturation dyes
