A pre-coelenterazine peptide comprising a modified A. victoria GFP having an amino acid sequence in which Ser.sup.65 is replaced with Tyr. There is further provided a polynucleotide encoding the pre-coelenterazine peptide, allowing synthesis of large, pure amounts of coelenterazine. ...http://www.google.fr/patents/US5741668?utm_source=gb-gplus-shareBrevet US5741668 - Expression of a gene for a modified green-fluorescent protein
Expression of a gene for a modified green-fluorescent protein