Receptors are employed to which one or more fluroescent molecules are bound. When the receptors are bound to the epitopic sites on the surface and light of predetermined wave length is directed toward the surface, the fluorescing molecules are activated and fluoresce....http://www.google.fr/patents/US3939350?utm_source=gb-gplus-shareBrevet US3939350 - Fluorescent immunoassay employing total reflection for activation
Fluorescent immunoassay employing total reflection for activation