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US007863050B2

(12) United States Patent

Domanico et al.

(io) Patent No.: (45) Date of Patent:

US 7,863,050 B2 Jan. 4, 2011

(54) METHODS AND COMPOSITIONS FOR

PURIFICATION OF NUCLEIC ACID FROM A
HOST CELL

(75)

Inventors: Michael J. Domanico, Longmont, CO
(US); Matt Myers, Boulder, CO (US);
Kristian Keane, Boulder, CO (US); Lisa
R. Braun, Denver, CO (US); Thomas
Kolzau, Hamburg (DE)

(73) Assignee: Qiagen North American Holdings,

Inc., Valencia, CA (US)

( * ) Notice: Subject to any disclaimer, the term of this patent is extended or adjusted under 35 U.S.C. 154(b) by 0 days.

(21) Appl.No.: 12/358,128

(22) Filed: Jan. 22, 2009

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Omm 1min 2min 3min 4min 5min 7min I0m;n *5min" RT incubation time between 0 cleg, incubation and spin

Figure 1B

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