Illlllllllllllllllllllllllllllllllllllllllllllllllll
US007575759B2
(12) United States Patent
Murphy et al.
(io) Patent No.: (45) Date of Patent:
US 7,575,759 B2 Aug. 18, 2009
(54) TISSUE ENGINEERING SCAFFOLDS
(75) Inventors: William L. Murphy, Chicago, IL (US);
Robert G. Dennis, Ann Arbor, MI (US);
David J. Mooney, Dexter, MI (US)
(73) Assignee: The Regents of the University of
Michigan, Ann Arbor, MI (US)
( * ) Notice: Subject to any disclaimer, the term of this patent is extended or adjusted under 35 U.S.C. 154(b) by 620 days.
(21) Appl.No.: 10/330,578
(22) Filed: Dec. 27, 2002
(65) Prior Publication Data
US 2004/0026811 Al Feb. 12, 2004
Related U.S. Application Data
(60) Provisional application No. 60/345,775, filed on Jan. 2, 2002.
(51) Int. CI.
A61F 2/00 (2006.01)
(52) U.S. CI 424/423
(58) Field of Classification Search None
See application file for complete search history.
(56) References Cited
U.S. PATENT DOCUMENTS
4,859,712 A * 8/1989 Cox 521/62
4,902,511 A * 2/1990 Kronman 424/423
5,514,378 A * 5/1996 Mikosetal 424/425
5,677,355 A * 10/1997 Shalabyetal 521/61
6,103,255 A * 8/2000 Leveneetal 424/426
6,306,424 Bl 10/2001 Vyakarnam et al 424/426
6,333,029 Bl 12/2001 Vyakarnam et al 424/93.1
6,337,198 Bl* 1/2002 Leveneetal 435/174
6,436,426 Bl * 8/2002 Liao et al 424/426
7,309,232 B2* 12/2007 Rutherford et al 433/226
2002/0005600 Al * 1/2002 Ma 264/49
OTHER PUBLICATIONS
"Humidity". Dictionary.com. Accessed online on Feb. 16, 2007. <http://dictionary.reference.com/browse/humidity>.* Murphy et al. Salt Fusion: An approach to improve pore interconnectivity within tissue engineering scaffolds. Feb. 2002. Tissue Engineering, vol. 8, No. 1. pp. 43-52.*
Aldini, N.N., et al., "Effectiveness of a bioabsorbable conduit in the repair of peripheral nerves," Biomaterials 17:959-962 (1996). Carrier et al., "Cardiac tissue Engineering: Cell seeding, cultivation parameters, and tissue construct characterization," Biotech Bioeng 64:580-589 (1999).
Chaignaud, B.E., et al., "The history of tissue engineering using synthetic biodegradable polymer scaffolds and cells," In: Atala,A., Mooney, D.J., eds. Synthetic biodegradable polymer scaffolds. Boston, MA: Birkhauser, 1997, pp. 1-14.
(Continued)
Primary Examiner—M P Woodward
Assistant Examiner—Casey S Hagopian
(74) Attorney, Agent, or Firm—Medlen & Carroll, LLP
(57) ABSTRACT
The present invention relates to methods and compositions for the production of scaffolds, such scaffolds to be used for a variety of purposes, including tissue engineering. More specifically, the present invention relates to the use of fused crystals, such as fused salt crystals to form a framework. The methods for producing the scaffolds of this invention improve the porosity, interconnectivity and ease of manufacture as compared to prior art methods.
16 Claims, 15 Drawing Sheets
OTHER PUBLICATIONS
Dennis et al., Excitability and isometric contractile properties of mammalian skeletal muscle constructs engineered in vitro, In Vitro Cell Dev Biol-Animal 36:327-335 (2000).
Evans, G.R.D., et al, "In vivo evaluation of poly (L-lactic acid) porous conduits for peripheral nerve regeneration," Biomaterials 20:1109-1115 (1999).
Evans, G.R.D., et al., "Tissue engineered conduits: the use of bioderadablepoly(D,L-latic-co-glycolic acid) scaffolds in peripheral nerve regeneration," In: Stark, G.E., Horch, R., Tanczos, E., Eds.. Biological Matrices and Tissue Reconstruction. Berlin:Springer, (1998) pp. 225-235.
Freed et al., "Neocartilage formation in vitro and in vivo using cells cultured on synthetic biodegradable polymers," JBiomed Mater Res 27:11-23 (1993).
Harris, L.D., et al., "Open pore biodegradable matrices formed with gas foaming," JBiomed Mater Res 42:396-402, (1998). Hutmacher, D.W., "Scaffolds in tissue engineering bone and cartilage," Biomaterials 21:2529-2543 (2000).
Ishaug-Riley et al., "Ectopic bone formation by marrow stromal osteoblast transplantation usinpoly (DL-lactic-co-glycolic acid) foams implanted into the rat mesentery," J Biomed Mater 36:1-8 (1997).
Kaufmann, P.M., et al., "Highly porous polymer matrices as a threedimensional culture system for hepatocytes," Cell Transplant 6:463468, (1997).
Kim et al., "Engineering smooth muscle tissue with a predefined structure," /Biomed Mater Res 41:322-332 (1998). Lu et al., "The importance of new proccessing techniques in tissue engineering," MRS Bull 21:28-32 (1996).
Ma, RX. and Choi, J., "Biodegradable polymer scaffolds with welldefined interconnected spherical pore network," TissueEng 7:23-33, (2001).
Mikos,A.G., etal. "Preparation and characterization of poly (L-lactic acid) foams" Polymer 35:10680-1077 (1994). Murphy, W.L. and Mooney, D.J., "Controlled delivery of inductive proteins, plasmid DNA and cells from tissue engineering matrices," / Periodontal Res 34:413-419 (1999).
Murphy, W.L. et al, "Growth of continuous bone-like mineral within porous poly (lactide-co-glycolide) scaffolds in vitro," / Biomed Mater Res 50:50-58 (2000).
Murphy, W.L., et al., "Sustained release of vascular endothelial growth factor from mineralized poly(lactide-co-glycolide) scaffolds fortissue engineering,"Biomaterials 21:2521-2527 (2000). Oberpenning et al., "De novo reconstitution of a functional mammalian urinary bladder by tissue engineering," Nat Biotech 7:149-155 (1999).
Shea, L.D., et al., "DNA delivery from polymer matrices for tissue engineering," Nat Biotech 17:551-554 (1999). Sheridan, M., et al. "Bioabsordable polymer scaffolds for tissue engineering capable of sustained growth factor delivery," / Control Rel 64:91-102 (2000).
Valentini, R.F., et al., "Collagen and laminin containing gels impede peripheral nerve regeneration through semipermeable nerve guidance channels," Exp Neurol 98:350-356 (1987). Van Vlack, L.H. "Elements of materials science and engineering," 4ed. Addison-Wesley Publishing Company, Reading, MA, pp. 120 &316, (1980).
van Wachem et al., "Absence of muscle regeneration after implantation of a collagen matrix seeded with myoblasts," Biomaterials 20:419-426(1999).
* cited by examiner
|
