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US007108891B2
(12) United States Patent ao) Patent No.: Us 7,108,891 B2
Chari et al. (45) Date of Patent: Sep. 19,2006
(54) RANDOM ARRAY OF MICROSPHERES
(75) Inventors: Krishnan Chari, Fairport, NY (US);
Tiecheng A. Qiao, Webster, NY (US);
Ramasubramaniam Hanumanthu,
Rochester, NY (US)
(73) Assignee: Eastman Kodak Company, Rochester, NY (US)
( * ) Notice: Subject to any disclaimer, the term of this patent is extended or adjusted under 35 U.S.C. 154(b) by 489 days.
(21) Appl. No.: 10/092,803
(22) Filed: Mar. 7, 2002
(65) Prior Publication Data
US 2003/0170392 Al Sep. 11, 2003
A method of making a microarray comprising the steps of: providing a support;
coating on the support a receiving layer to receive microspheres, the receiving layer being capable of undergoing sol/gel transition;
coating on the receiving layer a dispersion of microspheres in a carrier fluid, wherein the carrier fluid contains at least one crosslinking agent and is capable of solvating the receiving layer;
allowing the microspheres to partially submerge into the receiving layer;
creating conditions to induce sol/gel transition in the receiving layer, thus immobilizing the microspheres;
evaporating off the carrier fluid; and
allowing crosslinking reaction between the receiving layer and the crosslinker in the carrier fluid.
30 Claims, 5 Drawing Sheets
(4 of 5 Drawing Sheet(s) Filed in Color)
